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Citations for New England Biolabs :
951 - 1000 of 4067 citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... coli BL21(DE3) (NEB#C2527) was used for protein overexpression ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli NEB5-alpha (NEB#C2987) was used for the construction of all plasmids ...
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bioRxiv - Developmental Biology 2023Quote: ... coli (High Efficiency) (NEB, #C3019I) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... coli Top10 (NEB, Ipswich, US) and plated on LB plates (25 g/L Luria-Bertani agar ...
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bioRxiv - Microbiology 2023Quote: ... coli BL21(DE3) strain (NEB) were used ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells (New England Biolabs) were transformed with either of the five plasmids encoding CFP-E3-Switch ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli strain NEB 10β (NEB). L-arabinose (A3256-25 g) ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21(DE3) cells (NEB) were co-transformed with pBK plasmid for the expression of the evolved AcKRS3 synthetase39 ...
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bioRxiv - Developmental Biology 2023Quote: ... coli (Subcloning Efficiency) (NEB, #C2988J) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... coli DNA ligase (NEB, M0205L), E ...
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bioRxiv - Molecular Biology 2023Quote: ... Competent Escherichia coli DH5α (NEB) were transformed with the ligation products ...
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bioRxiv - Cell Biology 2023Quote: ... coli cells (New England Biolabs) were then transformed with assembled plasmids and grown on LB agar plates with appropriate antibiotics ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (New England Biolabs, C2987H) cells and grown with 100 μg/mL carbenicillin in TB (terrific broth) ...
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bioRxiv - Neuroscience 2023Quote: ... coli Poly-A Polymerase (NEB) for 1 hour at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... coli (C2987I, New England Biolabs). Positive colonies were screened by PCR using GoTaq G2 Hot Start green Master Mix (#M7422 ...
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bioRxiv - Microbiology 2024Quote: ... coli NEB 5-alpha (NEB) cells stored at −80 °C were thawed on ice and transformed with the ligation mixture according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... coli Poly (A) Polymerase (NEB). sgRNAs of waca or wacb were injected with Cas9 mRNAs into 1-cell stage embryos.
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bioRxiv - Biochemistry 2024Quote: ... coli polyA polymerase (NEB, M0276L), desalted on P30 columns ...
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bioRxiv - Biophysics 2024Quote: ... coli cells (New Englad Biolabs) and purified with QIAprep spin miniprep kits (Qiagen ...
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bioRxiv - Bioengineering 2024Quote: ... coli (New England Biolabs; C3040) made using the Mix&Go transformation kit (Zymo Research ...
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bioRxiv - Biophysics 2024Quote: ... coli 10-beta cells (NEB). Instead ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli poly(A) polymerase (NEB) at 37 °C for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... coli NEB5α (New England Biolabs) prior to transformation into the appropriate electrocompetent strains ...
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bioRxiv - Microbiology 2024Quote: ... coli DH5lll (New England Biolabs) following the manufacturer instructions ...
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bioRxiv - Synthetic Biology 2024Quote: Escherichia coli strain K12 (NEB Turbo Competent E ...
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bioRxiv - Microbiology 2024Quote: ... 5α Competent Escherichia coli (NEB) cultured in lysogeny broth was used for plasmid preparation.
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bioRxiv - Immunology 2024Quote: ... coli (#C3040H, New England Biolabs) were transformed with the generated constructs ...
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bioRxiv - Cell Biology 2024Quote: ... coli RNase HII (NEB #M0288). Reactions were quenched by addition of EDTA to 50 mM and proteins were removed by treatment with proteinase K (20 units/ml ...
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bioRxiv - Microbiology 2024Quote: ... coli cells (New England Biolabs). Recombinant expression for assessment of BcsF roles in macrocomplex assembly was carried out in a T1 phage-resistant 11bcs BL21*(DE3 ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli (High Efficiency) (#C3019H, NEB) or Electroporation Protocol (#C3020 ...
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bioRxiv - Cell Biology 2020Quote: 100μg of each P13 and P40 sample was resuspended with 20μL of 1X glycoprotein denaturing buffer (New England BioLabs) and incubated at 100°C for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... the tbb-2 3’UTR sequence was amplified from genomic DNA using PCR and primers 5’-tggatgaactatacaaatagatgcaagatcctttcaagca-3’ and 3’-aggttttcaccgtcatcacccgcgaaaaacccatgtaagt-5’ and the vector containing the sequence of pie-1p::his-15::gfp amplified from plasmid containing pie-1p::his-15::gfp::egg-6 3’UTR using PCR and primers 5’-ggtgatgacggtgaaaacct-3’ and 3’-ctatttgtatagttcatccatgcc-5’ were used to generate pie-1p::his-15::gfp::tbb-2 3’UTR by using Gibson assembly (NEB E2611). To add the wild type or mutant 3xmir-51 seed sequences ...
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bioRxiv - Developmental Biology 2021Quote: 4mC and 5mC spike-in controls were generated from pUC19 (NEB) PCR product synthesized with N4-methyl-dCTP (4mdCTP ...
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bioRxiv - Cell Biology 2022Quote: ... the sequence encoding amino acids 1-490 was amplified with NdeI and EcoRI overhangs and inserted into a modified backbone based on pSNAP-tag(T7)2 (NEB #N9181S) before a SNAPf-EGFP-6His tag (Budaitis et al. ...
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bioRxiv - Molecular Biology 2021Quote: Myc-Tag (9B11) antibody (NEB 2276 S) and mouse IgG (Santa Cruz sc-2025 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-SNAP-tag (New England Biolabs, #P9310S) and anti-Cyclin D1 (BD Bioscience ...
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bioRxiv - Cell Biology 2022Quote: ... or SNAP-tag (New England BioLabs, N9181S) were inserted into the retroviral plasmids pMRX-IP (harboring a puromycin-resistant marker ...
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bioRxiv - Genetics 2020Quote: ... anti-MYC-tag (Cell Signaling Technology/NEB) at 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... SNAP-tag DNA (pSNAPf, New England Biolabs) and β-Actin DNA (Actin mRFP-PAGFP was a gift from Guillaume Charras & Tim Mitchison ...
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bioRxiv - Microbiology 2024Quote: ... the SNAP tag (New England Biolabs #N9183S) was inserted after the Ptet or Plac promoter with the appropriate primers (supplementary methods) ...
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bioRxiv - Cell Biology 2023Quote: ... and SNAP-tag (New England BioLabs, N9181S) were used for tagging ...
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bioRxiv - Cell Biology 2020Quote: ... S141A ABHD11 and H296A ABHD11 with C-terminal eGFP tags or HA tags were created using NEBuilder HiFi (NEB). ABHD11 was also cloned into a transfection vector ...
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bioRxiv - Cancer Biology 2021Quote: ... Biotinylated nucleotides from the non-ligated DNA ends were removed by incubating the Hi-C libraries (2 μg) in the presence of 6 U of T4 DNA polymerase (NEB; Cat#: M0203L) in NEBuffer 2.1 supplied with 0.025 mM dATP (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... mutations were inserted into the open reading frame of Vesicular stomatitis virus glycoprotein VSV-G using the Q5 SDM kit (NEB). Myc epitope-tagged SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 40 – 50 µg of protein lysate from each sample was denatured in 1X Glycoprotein Denaturing Buffer (Cat.#B1704S; New England Biolabs) with 20 µL total volume at 100°C for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... lysate volume equivalent to 20-40 µg total protein was first denatured at 100°C for 10 min in 1X glycoprotein denaturation buffer (NEB). Endo H digestion was performed at 37°C for 1h in the presence of 1X GlycoBuffer 3 and 1 µL of Endo H enzyme (NEB) ...
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bioRxiv - Cancer Biology 2022Quote: ... AAK1(S-676D/E/A) and UVRAG (T-518D/E/A) using Q5-site directed mutagenesis Kit (NEB). Manufacturer’s instructions for mutagenic primer design were followed ...
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bioRxiv - Biochemistry 2021Quote: ... 25 U Hi-T7 RNA polymerase (NEB #M0658), 250 nM RNaseAlert™ QC System v2 (ThermoFisher ...
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bioRxiv - Plant Biology 2020Quote: ... coli ER2566 cells (New England Biolabs). Expression of CBC-Qprot as a 15N-labeled protein and purification via Co-NTA affinity chromatography and electroelution was performed as described previously for the photosynthesis QconCAT protein (PS-Qprot ...
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bioRxiv - Cancer Biology 2021Quote: ... coli poly(A) polymerase (NEB Biolabs), and capped and 2’-O-methylated with Vaccinia Capping System (NEB Biolabs) ...