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Citations for New England Biolabs :
951 - 1000 of 2003 citations for Recombinant Human Interleukin 2 Receptor Alpha Fc chimera since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... rRNA depletion and library preparation for sequencing was completed with the NEBNext protocol (‘Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
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bioRxiv - Developmental Biology 2020Quote: A digoxin-labeled human H19X probe was transcribed in situ from a linear template plasmid by T7 RNA polymerase (NEB, UK) with Digoxin-labeled Uridine (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... per 1g of wet cell weight and 1 Unit of 30 Units/μL RNase Inhibitor (from human placenta; New England Biolabs (NEB), No ...
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bioRxiv - Cell Biology 2021Quote: ... ORF was amplified from MCF10A human mammary cells using specific primers (listed in Table S2) with Q5 High-Fidelity 2X Master Mix (New England BioLabs, # M0492), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... RNA-seq libraries were prepared according to the protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) ...
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bioRxiv - Developmental Biology 2023Quote: Human and bovine XIST E repeats were PCR amplified from cDNA generated from either total RNA of Ishikawa (human) or bovine stromal cells (Supplementary Table S4) with the Q5 High-Fidelity DNA polymerase (NEB, UK) or the non-proofreading Taq DNA Polymerase (EP040 ...
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bioRxiv - Immunology 2023Quote: ... Ribosomal RNA from 1000 ng total extracted RNA was depleted using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat; New England Biolabs Inc.). The remaining RNA was used to produce the sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... W165F and S169A) were introduced into human PSEN1 cDNA cloned into the pMSCVpuro vector using Q5 Site-Directed Mutagenesis Kit (NEB BioLabs) according to the standard protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 μg DNA spiked with methylation standards was digested with SmaI endonuclease (NEB) at 25°C for 8 h ...
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ISL2 is an epigenetically silenced tumor suppressor and regulator of metabolism in pancreatic cancerbioRxiv - Molecular Biology 2020Quote: ... Q5-high Fidelity 2× master mix was used as polymerase from NEB (# M0429L). PCR condition for the 1st PCR was ...
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bioRxiv - Developmental Biology 2021Quote: ... 200 ng of PCR products in 1X NEB buffer 2 (New England Biolabs) were hybridized under the following conditions ...
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bioRxiv - Genomics 2020Quote: ... 2 μL 10 mM dGTP and 10 mM dTTP (stock solutions: NEB, N0446), 10 μL 10x T4 DNA Ligase Reaction Buffer (NEB B0202S) ...
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bioRxiv - Developmental Biology 2022Quote: ... washed before either incubation with SNAP-Cell TMR-Star at 2 μM (NEB) and SNAP-Surface Block at 13 μM (NEB ...
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bioRxiv - Biophysics 2021Quote: ... following incubation for ∼2 hours of 10 mg/ml BSA (New England Biolabs) diluted in buffer A containing 20 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μL of 1× PNK mix (2 μL of 10× PNK buffer (NEB), 2 μL ATP (SCP801 ...
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bioRxiv - Genomics 2022Quote: ... 1 or 2 μl of 1U/μl USER® II enzyme (M5505S, NEB) and nuclease-free water to made up to 10 μl ...
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bioRxiv - Molecular Biology 2021Quote: ... 2X hybridisation buffer (4X SSC, 20% dextran sulphate, 2 mg/mL BSA (NEB), 1/10 volume nuclease free water and 1/10 volume vanadyl-ribonucleoside complex (VRC ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 mM EGTA and 2 mM Vanadyl Ribonucleoside complex (VRC, New England Biolabs), washed three times with 70% ice-cold ethanol and kept at −20°C.
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bioRxiv - Developmental Biology 2019Quote: ... 2 mM dithiothreitol) with or without 50 µM ATP (P0756S, New England Biolabs) at 30°C for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... 5 µl was used for BglII digestion (NEB, 2 hours at 37°C) and the products were loaded on a 2% agarose gel to confirm the pAS insertion ...
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bioRxiv - Cancer Biology 2021Quote: ... Sequencing libraries were prepared using NEBNext HiFi 2× PCR Master Mix (NEB, M0541L) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... Dynein-dynactin-adaptor complexes were labelled with 2 µM SNAP-TMR dye (NEB) during the isolation procedure from rat brain lysate (McKenney et al. ...
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bioRxiv - Systems Biology 2020Quote: ... 2 mL of ligation mix (500 μL of T4 Ligase Buffer 10x (NEB), 100 μL of T4 DNA Ligase (400 U/μL ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR round 1 and 2 were performed using Q5-High Fidelity polymerase (NEB) for 6 (FS231 and FS232 ...
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bioRxiv - Cell Biology 2021Quote: ... and lysate was incubated 37°C 60 min in 2 units CIP (NEB) per 50 μL reaction containing 50μg of total protein ...
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bioRxiv - Plant Biology 2020Quote: ... 2 μg of total RNA was treated with DNase I (New England BioLabs) and then cleaned with RNA Clean and Concentrator-5 (Zymo Research) ...
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bioRxiv - Biochemistry 2022Quote: ... and PCR library amplification (KAPA library amplification primers; NEB Ultra 2× master mix), followed by double-sided SPRI size selection and a final 0.9× SPRI purification ...
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bioRxiv - Neuroscience 2022Quote: ... 25 μl NEBNext High-Fidelity 2× PCR Master Mix (New England Biolabs, M0541S), 9 μl of unique ...
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bioRxiv - Plant Biology 2021Quote: ... This 2 kb fragment was subsequently cloned into the Eco53kI restriction enzyme (NEB) site of pK18B-E (Jayaraman et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... 2μl 10 mM dNTPs and 1μl Bst 2 WarmStart DNA polymerase (NEB M0538S) was added and sample incubated 30 min at 65°C before precipitation with 12.5 μl 10 M NH4OAc ...
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bioRxiv - Biophysics 2021Quote: ... following passivation for ~2 hours of 10 mg/ml BSA (New England Biolabs). After removing non-adhered BSA by washing the flow cell with PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 ul RNaseOUT and 1ul T4 RNA ligase 2 truncated K227Q (NEB M0351). Samples were then washed twice with PNK buffer ...
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bioRxiv - Genomics 2019Quote: ... in a 50⍰ μl reaction with 2× PCR master mix (NEB cat. M0541). PCR cycled as ...
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bioRxiv - Biochemistry 2019Quote: ... PCR was performed using Q5 Host Start High- Fidelity 2× Master Mix (NEB), and 150 ng Genomic DNA was chosen as the template ...
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bioRxiv - Genomics 2019Quote: ... DNA was next dephosphorylated with 2 μl calf intestinal alkaline phosphatase (NEB M0290S) and incubated at 50°C for 60 min and immediately purified by 2x AmpureXP beads ...
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bioRxiv - Genetics 2019Quote: ... the vector was treated with 2 μl of calf intestinal alkaline phosphatase (NEB) at 37 °C for 30 min ...
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bioRxiv - Genomics 2019Quote: ... 2 µg of genomic DNA was digested with 20 units of MspI (NEB) overnight at 37C in a volume of 100 µl ...
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bioRxiv - Microbiology 2019Quote: ... Growth was monitored every 2 min using a Cell Growth Quantifier (Aquila Biolabs).
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bioRxiv - Microbiology 2020Quote: ... lot 0091412 and the NEBNext multiplex Oligos for Illumina (Set 2, NEB #E7500) lot 0071412 ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.6 and incubated overnight with 2 µl PNGase F (New England BioLabs) and 0.5 µl sialidase A (Prozyme) ...
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bioRxiv - Genomics 2020Quote: ... 2 μl 10 mM dGTP and 10 mM dTTP (stock solutions: NEB, #N0446), 10 μl 10x T4 DNA Ligase Reaction Buffer (NEB #B0202S) ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were treated with 2 μl of proteinase K (NEB, 800 units/ml) for 20 min at 37 °C and 5 ul of each sample was applied onto 20% denaturing gels (20% acrylamide/bis-acrylamide ...
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bioRxiv - Plant Biology 2020Quote: ... 2 µg of total RNA was treated with DNase I (New England BioLabs) and then cleaned with RNA Clean and Concentrator-5 (Zymo Research) ...
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bioRxiv - Cell Biology 2021Quote: ... containing 2□ μl of 1× NEB Next Cell Lysis Buffer (New England Biolabs). FACS sorting was performed with a BD Influx sorter (BD Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... and then digested with 2 μL Nucleoside Digestion Mix (#M0649, New England Biolabs) in a 50 μL reaction incubated at 37 °C overnight ...
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bioRxiv - Microbiology 2020Quote: ... and 0.15mM of 7-deaza-dGTP (7-deaza-2′-deoxyguanosine 5′-triphosphate, NEB).
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bioRxiv - Immunology 2020Quote: 2 μg of base expression vectors were digested using NotI-HF (NEB # R3189S) and SmaI (NEB # R0141S ...
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bioRxiv - Genomics 2020Quote: ... 10 µL of 2% bovine serum albumin (New England Biolabs, cat. no. B9000S), 0.3 µL of 1M spermine (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... 25 μl of nuclease free water and 2 μl of DnaseI (M0303S, NEB) were added to degrade the DNA template ...
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bioRxiv - Cancer Biology 2022Quote: ... and NEBNext Multiplex Oligos for Illumina (NEB, Set 1 #E7335S, Set 2 #E7500S). ChIP libraries were done following NEB’s guidelines (NEB ...