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Citations for New England Biolabs :
951 - 1000 of 1424 citations for Recombinant Enhanced Green Fluorescent Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... and (MBP)2-HSPC300 proteins were purified through amylose beads (New England Biolabs). The Sra1/Nap1 dimer was obtained by co-expressing His6-Sra1 and untagged Nap1 in Tni insect cells (Expression Systems) ...
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bioRxiv - Plant Biology 2023Quote: ... The MBP-fused protein was purified with Amylose Resin (E8021V; New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... the protein-bound RNA was dephosphorylated with Quick CIP (M0525S, New England Biolabs) for 10 min in 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... protein samples were incubated with 500 U of PNGase F (New England Biolabs) or Endo H (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... The Color Protein Standard Broad Range (10–250 kDa) (New England BioLabs Inc.) was used as protein molecular weight ladder ...
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bioRxiv - Biophysics 2024Quote: ... The lysate was pre-cleared using protein G magnetic beads (New England Biolabs). The cleared supernatant was then incubated with 3 µg anti-myc antibody (SIGMA M4439 monoclonal anti c-myc or Abcam ab206486 rat mAb to myc tag (9E10) ...
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bioRxiv - Cell Biology 2021Quote: ... Carla Green as in (Rosensweig et al., 2018) and the modified using Q5 Site-Directed Mutagenesis (New England Biolabs Inc., Ipswich, MA, cat # E0554S) to create the CRY2 TCGA mutants ...
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bioRxiv - Cell Biology 2020Quote: ... dCas9-SNAP protein samples were incubated with SNAP-Surface Alexa Fluor 647 (NEB S9136) at 37°C for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... The proteins were expressed in BL21 DE3 competent cells (New England Biolabs, Ipswich, MA). Protein purification was undertaken using nickel-affinity chromatography (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... Mutant protein expression vectors were generated using the Q5 Mutagenesis Kit (New England BioLabs) and proteins overexpressed and purified the same as wild-type.
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bioRxiv - Cancer Biology 2021Quote: ... Precipitated proteins were resuspended in 150 μL of Glycoprotein Denaturing Buffer (New England Biolabs) and incubated at 100 °C for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... reduced and alkylated and proteins were digested with first PNGase F (New England BioLabs), endoproteinase Lys-C (Promega ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... against a Colour Prestained Protein Standard (Broad Range 10-250 kDa, New England BioLabs) and resolved by electrophoresis for 70 minutes at 150-180V ...
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bioRxiv - Neuroscience 2020Quote: ... AP fusion proteins are quantified using calf intestinal alkaline phosphatase (CIP, New England Biolabs) activity ...
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bioRxiv - Cell Biology 2021Quote: ... and then resuspended in phosphatase buffer with 200U lambda protein phosphatase (New England Biolabs) at 30 °C for 20 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein sample was concentrated by ultrafiltration and incubated with PNGaseF (New England Biolabs) overnight.
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bioRxiv - Biophysics 2022Quote: ... purified proteins were labelled with SNAP-Surface Alexa Fluor 647 tag (New England Biolabs) following manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... The denatured proteins were then supplemented with 500 U of EndoH-MBP (EndoHf, NEB) and incubated for 45 min at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... All other proteins were expressed in Escherichia coli BL21(DE3) cells (New England BioLabs).
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bioRxiv - Cell Biology 2019Quote: ... immunoprecipitated beads were incubated with 40 Units of lambda protein phosphate (New England Biolabs) in a 50 μl reaction ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein was degraded by adding 50 µl of 20mg/ml proteinase K (NEB, P8102), 50 µl of 10% SDS and incubated at 55°C for 30 minutes ...
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bioRxiv - Immunology 2020Quote: For embryo microinjection the annealed sgRNA was complexed with Cas9 protein (New England Biolabs) at RT for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2-bound proteins were eluted by adding 7.5 μl RNase H (NEB), 2 μl TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... one was treated with 400U Lambda protein phosphatase (Lambda PP, New England Biolabs, #P0753S) in 50µl phosphatase assay buffer ...
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bioRxiv - Immunology 2020Quote: ... For embryo microinjection the annealed sgRNA was complexed with Cas9 protein (New England Biolabs) at room temperature for 10 min ...
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bioRxiv - Genomics 2021Quote: ... Proteins were then mixed with Buffer A supplemented with S-adenosyl-methionine (NEB B9003S) containing 1 ug of either naked unmethylated DNA (Plasmid ASP3552 ...
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bioRxiv - Cancer Biology 2022Quote: ... and biotin-labelled proteins were isolated by immunoprecipitation using streptavidin magnetic beads (NEB, S1420S) in an immunoprecipitation buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... aliquots of S proteins were digested respectively using alpha lytic protease (New England BioLabs), chymotrypsin (Athens Research and Technology) ...
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bioRxiv - Microbiology 2022Quote: ... and protein purification was carried out according to the manual of Amylose Resin (NEB). The protein ...
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bioRxiv - Microbiology 2022Quote: ... The final injection mix contained 300 ng/μL spCas9 protein (M0386, New England Biolabs), 40 ng/μL of each sgRNA (3 targeting exon 5 and 7 of Dicer2 and 4 targeting the kmo gene ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein amounts for deadenylation assays were estimated against BSA standards (New England Biolabs, B9000S).
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bioRxiv - Biochemistry 2024Quote: ... Bound proteins were eluted by digestion with 500 units micrococcal nuclease (New England Biolabs) for 10 minutes at 37°C in 25 mM HEPES-KOH (pH 7.6) ...
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bioRxiv - Biochemistry 2024Quote: ... The proteins were purified on the amylose resin (New England Biolabs, Ipswich, MA, USA) and stored at −20 °C in 25 % glycerol ...
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bioRxiv - Cell Biology 2023Quote: ... cleaved protein product was passed through Ni-NTA resin and amylose resin (E8021, NEB) to remove the TEV protease and HIS-MBP tag ...
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was labeled with SNAP-Surface Alexa Fluor647 dye (New England Biolabs). The fractional dye labeling and DNA binding activity were assessed as previously described [12].
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bioRxiv - Developmental Biology 2023Quote: ... Injection mixtures containing 500 ng/µl of Cas 9 protein (NEB; Cat. no.: M0641) and 300 ng/µl of guide RNA were prepared and injected into eggs within 4 hours of egg laying ...
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bioRxiv - Physiology 2023Quote: ... for every 2 µg of fusion protein in 1X TEV buffer (Catalog P8112S, NEB) for 16h at 30°C.
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bioRxiv - Physiology 2023Quote: ... The proteins were then purified by affinity chromatography using amylose resin (Catalog E8021L, NEB). The amylose resin was washed and pre-equilibrated in 20 mM tris ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were determined via SDS–PAGE and Coomassie staining using BSA (NewEngland Biolabs) as a standard ...
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bioRxiv - Immunology 2023Quote: ... with a mixture of 0.33 µM EnGen Cas9 protein (New England Biolabs, Cat.No. M0646T), two Cas9 guides RNA ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then degraded by addition of 1 μL of Proteinase K (NEB #P8107) and incubation at 56°C for 30 min ...
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bioRxiv - Immunology 2023Quote: For embryo microinjection the annealed sgRNA was complexed with Cas9 protein (New England Biolabs) at room temperature for 10 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Assays were set-up using PURExpress in vitro protein synthesis kit (New England Biolabs) with a cloned full-length DHFR template ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were purified on amylose resin (New England Biolabs, Ipswich, MA, USA; Cat# E8021) and eluted with maltose using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µl of a broad range color pre-stained protein standard (New England BioLabs) was added to the outer lanes ...
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bioRxiv - Cell Biology 2023Quote: ... immunoprecipitated beads were incubated with 40 Units of lambda protein phosphate (New England Biolabs) in a 50 μl reaction ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg of lysates were deglycosylated using Protein Deglycosylation Mix II (NEB Cat# P6044S). In short ...
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bioRxiv - Plant Biology 2024Quote: ... The protein samples were treated with Endo Hf (New England BioLabs, Ipswich, MA, USA) at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: For lambda phosphatase assay the reactive from Lambda Protein Phosphatase (Lambda PP) (P0753L, NEB) were used and followed the protocol from the manufacturer.
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bioRxiv - Microbiology 2020Quote: Fetuin was deglycosylated under denaturing conditions using Protein Deglycosylation Mix II kit (New England Biolabs) according to the manufacturer’s protocol ...