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Citations for New England Biolabs :
51 - 100 of 3214 citations for S + α 1 Naphthyl ethylamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... and 640 µM S-adenosylmethionine (SAM) (New England Biolabs). The reaction was incubated for 5 min at 37 °C and placed on ice until grids were prepared.
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bioRxiv - Genomics 2019Quote: ... 96 μM S-adenosylmethionine (SAM; New England Biolabs, NEB), and 200 U M ...
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bioRxiv - Genomics 2019Quote: ... 96 μM S-adenosylmethionine (SAM; New England Biolabs, NEB), and 200 U M ...
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bioRxiv - Microbiology 2021Quote: ... which were incubated with 1 μg/ml full-length S protein with His tag that had been pretreated with or without FXa (P8010L, NEB) for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... accordingly to manufacturer’s instructions and 1 μg of RNA was reverse-transcribed into cDNA using M-MuLV reverse transcriptase (New England Biolabs) and random hexamers ...
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bioRxiv - Genomics 2020Quote: ... Methylation reactions were performed in a 100 uL reaction with Methylation Reaction buffer (1X CutSmart Buffer,1 mM S-adenosyl-methionine (SAM, New England Biolabs)) and incubated with 2.5 uL EcoGII at 37°C for 30 minutes ...
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Promoter-adjacent DNA hypermethylation can downmodulate gene expression: TBX15 in the muscle lineagebioRxiv - Molecular Biology 2022Quote: ... by incubating the DNA construct (1 μg) with 4 units of SssI methylase and 160 μM S-adenosylmethionine (New England Biolabs) for 4 h at 37°C or mock-methylating by incubating in the absence of S-adenosylmethionine ...
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bioRxiv - Molecular Biology 2021Quote: Experiments were performed in Escherichia coli DH5-α (NEB), grown in LB-Lennox (Oxoid ...
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bioRxiv - Microbiology 2022Quote: ... 0.5μl [α-32P]rUTP and 1μl T7 polymerase (NEB). After incubation at room temperature for 2 hours the NS5B RdRp reactions were placed on ice while the T7 reactions were supplemented with 1 unit RNase-free DNase and incubated at 37°C for 20 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... two guide RNAs and the Cas9 enzyme (S. pyogenes, NEB) were diluted to a final concentration of 20 ng/µl each in injection buffer ...
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bioRxiv - Microbiology 2020Quote: ... 45 s using the NEBNext Magnesium RNA Fragmentation Module (NEB) followed by RNA purification with the Zymo RNA Clean & Concentrator kit ...
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bioRxiv - Plant Biology 2022Quote: ... enzyme (MBP-DcATX1C) and S-adenosyl-L-methionine (SAM; NEB) were incubated for 0h ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5 μl of 32 mM S-adenosylmethionine (SAM; NEB, B9003) and 50 μl of M.CviPI (NEB ...
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bioRxiv - Microbiology 2019Quote: ... Typhimurium NCTC 12023 and the vector including the Tet-on system aph tetR PtetA present on p4392 occurred using oligonucleotides as listed in Table S 1 and purified by PCR purification (NEB Monarch). The PCR product encoding for sadBA and the PCR product from vector p4392 were assembled by Gibson assembly according to manufactureŕs protocol (NEB Monarch) ...
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bioRxiv - Neuroscience 2022Quote: ... Enzymes used included α mannosidase (P0768, New England Biolabs [NEB]), α(1-3,4 ...
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bioRxiv - Neuroscience 2022Quote: ... Enzymes used included α mannosidase (P0768, New England Biolabs [NEB]), α(1-3,4 ...
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bioRxiv - Microbiology 2023Quote: ... The product was then transformed into DH5-α cells (NEB). After plating onto LB-amp plates ...
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bioRxiv - Microbiology 2024Quote: ... Competent strains of Escherichia coli DH5-α (New England Biolabs), used for DNA cloning and plasmid propagation ...
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bioRxiv - Cell Biology 2020Quote: ... or the LunaScript RT Super Mix Kit (NEB # E3010(S/L), Ipswich ...
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bioRxiv - Molecular Biology 2020Quote: ... S protein were first incubated with either 1000U/mL PNGase F (NEB) or deactivated PNGase F at 37 °C for 14 h ...
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bioRxiv - Biochemistry 2020Quote: ... 30 nM dCas9 protein (dCas9 Nuclease, S. pyogenes, M0652, New England Biolabs), 1–4× 104 Beads@oligo ...
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bioRxiv - Biophysics 2020Quote: ... Deglycosylated trastuzumab was obtained by treatment with Endo S (New England BioLabs) at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 mM Spermidine) supplemented with 0.8 mM S-adenosyl-methionine (NEB B9003S). A negative control was prepared without MTase ...
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bioRxiv - Molecular Biology 2022Quote: ... and α-N-acetyl-galactosaminidase (New England Biolabs, 10 U/μg protein). Removal of sialic acids was performed using the α2-3,6,8 neuraminidase (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... the isolated glycoproteins are treated by α-2,3/6/8 neuraminidase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the product was transformed into DH5-α cells (NEB, Cat. No. C2987H). Following culture on LB-ampicillin plates (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... The bead bound α-βγδε fragments were digested with BsaI-HF (NEB) to prepare the 3’ end of the DNA fragments for the subsequent ligation step.
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bioRxiv - Biochemistry 2020Quote: Three microgram of full-length S protein were treated with PNGase F (NEB) for N-glycan release according to the manufacture’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... 4% Glycerol and 0.1 mM DTT) with 75 µM S-Adenosylmethionine (SAM, NEB), varying amounts ssRNA and duplex RNA (see above) ...
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bioRxiv - Bioengineering 2022Quote: Escherichia coli (E. coli) strain NEB 5-α (New England Biolabs, Hertfordshire, UK) was used for generation of genetic constructs ...
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bioRxiv - Molecular Biology 2023Quote: ... Editing activity of α-synCas was evaluated using EnGen Mutation Detection Kit (NEB). Briefly ...
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bioRxiv - Genomics 2021Quote: ... Proteins were then mixed with Buffer A supplemented with S-adenosyl-methionine (NEB B9003S) containing 1 ug of either naked unmethylated DNA (Plasmid ASP3552 ...
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bioRxiv - Biochemistry 2022Quote: ... aliquots of S proteins were digested respectively using alpha lytic protease (New England BioLabs), chymotrypsin (Athens Research and Technology) ...
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bioRxiv - Genomics 2023Quote: ... as described before. Cas9-gRNA complex (6.6 uM S. pyogenes Cas9 Nuclease (NEB, M0386T), 20 uM gRNA ...
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bioRxiv - Microbiology 2023Quote: ... All S recombinants were constructed via gene fragment Assembly (New England Biolabs, catalog #: E2621S).
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bioRxiv - Molecular Biology 2024Quote: ... 2.13 mM S-adenosylmethionine) and 50uL of 4,000U/mL M.CviPI GpC methyltransferase (NEB, M0227L). Each methylation reaction was then incubated for at 37 °C shaking at 1000 rpm for 7.5 minutes (unless specified otherwise ...
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bioRxiv - Developmental Biology 2019Quote: ... RNAs were cap-labeled with α-32P-GTP using the Vaccinia capping system (NEB) and gel purified prior to use ...
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bioRxiv - Immunology 2021Quote: ... Enzymatic assays for PNGase F and Endo S were performed according to manufacturer’s instructions (NEB). The effect of the enzymatic assays was either analyzed by Coomassie staining or immunoblotting.
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bioRxiv - Physiology 2022Quote: ... DNA block(s) were assembled into the SapI-linearized AAV vector using the NEBuilder (NEB).
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bioRxiv - Cancer Biology 2020Quote: ... Five µl of EcoRI buffer supplemented with 80 µM S-adenosyl methionine (New England Biolabs, Germany) and 2.5 µl BpuEI (5 U/µl ...
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bioRxiv - Biophysics 2020Quote: ... The primers above were designed with overlaps for Gibson Assembly according to manufacturer’ s specifications (NEB). The three PCR generated fragments were assembled in the presence of pUC18 digested with NdeI and PstI (NEB ...
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bioRxiv - Genomics 2021Quote: ... together with the Cas9 protein (EnGen® Cas9 NLS, S. pyogenes, M0646M, NEB, Ipswich, MA, USA) was injected into zebrafish embryos at one-cell stage ...
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bioRxiv - Developmental Biology 2022Quote: ... and 4 mM DTT) supplemented with 200 μM cold SAM (S- adenosyl methionine) (NEB; for immunoblotting) or 1 µCi 3H-labelled SAM (PerkinElmer ...
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bioRxiv - Microbiology 2021Quote: ... 10 μL Luna Universal qPCR Master Mix (New England Biolabs Inc., Bionordika Denmark A/S, Denmark), 1.4 μL of each primer (10 μM) ...
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bioRxiv - Genetics 2023Quote: ... 0.5 μl of Hia5 MTase (100 U) and 1.5 μl 32 mM S-adenosylmethionine (NEB B9003S) (0.8 mM final concentration ...
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bioRxiv - Microbiology 2020Quote: ... The ligated product was then chemically transformed into Escherichia coli DH5-α competent cells (NEB, Australia) following the company’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Human α-synuclein cDNA from a pcDNA3.1 plasmid was restriction digested with EcoRI and HindIII (NEB), purified and ligated in the same sites of the pAAV2.5-THP backbone to generate the pAAV2.5-TH-α-syn plasmid ...
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bioRxiv - Biochemistry 2023Quote: Plasmids containing the α and β genes were freshly transformed in E.Coli BL21-DE3 cells (NEB). Transformed cells were plated on LB agar plates containing 100ug/ml Carbenicillin and grown overnight at 370C overnight ...
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bioRxiv - Bioengineering 2020Quote: ... SARS-CoV-2 S was amplified by PCR (Q5 High-Fidelity 2X Master Mix, New England Biolabs) from pUC57-nCoV-S (kind gift from Jonathan Abraham lab) ...
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bioRxiv - Systems Biology 2020Quote: SARS-CoV-2 S was amplified by PCR (Q5 High-Fidelity 2X Master Mix, New England Biolabs) from pUC57-nCoV-S (gift of Jonathan Abraham) ...