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Citations for New England Biolabs :
51 - 100 of 9335 citations for Mouse Thymosin Beta 4 TMSB4X ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... The product was transformed into 10-beta Electrocompetent E.coli (NEB), by electroporation with 2.0kV ...
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bioRxiv - Microbiology 2022Quote: ... coli (NEB 10-beta electrocompetent cells, New England BioLabs C3020K) and plated at a target bottleneck of 100,000 variants per duplicate library ...
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bioRxiv - Immunology 2022Quote: ... coli (NEB 10-beta electrocompetent cells, New England BioLabs #C3020K), and bottlenecked to ∼1 × 105 cfus (an average of >25 barcodes per single-mutant) ...
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bioRxiv - Genomics 2023Quote: ... followed by transformation into 10-beta competent cells (NEB, C3020) using the Gemini X2 machine (BTX) ...
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bioRxiv - Immunology 2024Quote: ... we electroporated 100 µl of NEB 10-beta (NEB, #C3020K) in a BioRad electroporator using the preset E ...
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bioRxiv - Synthetic Biology 2024Quote: ... commercial 10-beta competent cells (New England Biolabs, Ipswitch, MA) were transformed with plasmids carrying a canvas repeat sequence-targeting sgRNA cassette driven by strong constitutive promoter apFAB36 [21] ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Developmental Biology 2020Quote: ... and transformed into NEB 10-beta Competent E.coli (NEB, Ipswich, MA). Colonies positive for the Lgals3-R200S allele by PCR were grown overnight at 37 °C with nutation at 200 rpm ...
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bioRxiv - Evolutionary Biology 2021Quote: ... coli K-12 DH10B (Neb 10-beta, NEB catalogue no. C3019H), E ...
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bioRxiv - Microbiology 2020Quote: ... and the assembly reaction used to transform E.coli (NEB 10 beta). Colonies positive by PCR screening using primers that flank the cloning site were sequenced across the entire S coding region and a single positive isolate adopted for all further manipulations.
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bioRxiv - Microbiology 2021Quote: Escherichia coli (NEB® 10-beta Electrocompetent E. coli or NEB® 5-alpha Competent E ...
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bioRxiv - Microbiology 2021Quote: ... were cloned into the pCR-Blunt-II vector using the PCR Blunt Topo kit according to the manufacturer’s instructions and transformed into 10-beta CaCl2-chemically competent bacteria (originally NEB, generated in-house). Following amplifications of colonies and purification of plasmids using a QiAprep Mini kit (Qiagen) ...
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bioRxiv - Genetics 2019Quote: ... Clones were transformed into electrocompetent 10-Beta cells (New England Biolabs # C3020) using a 96-well plate electroporator using fresh electroporation plates (BTX 45-0450-M) ...
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bioRxiv - Genetics 2019Quote: ... coli strains NEB10®-beta (New England BioLabs Inc., Ipswich, MA, USA), and XL1-blue (Agilent Technologies ...
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bioRxiv - Biophysics 2021Quote: ... the library was transformed into elec-trocompetent NEB 10-beta cells (NEB) to yield ¿1000x transformants per gene ...
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bioRxiv - Genetics 2019Quote: ... and transformed into high efficiency 10-beta competent E.coli (New England BioLabs) to generate pDestTol2pA2_ubi:f5-p2A-EGFP ...
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bioRxiv - Synthetic Biology 2021Quote: ... NEB 10-beta/Stable Outgrowth Medium (450 μL; B9035S, New England Biolabs) was added to each aliquot and the mixture was shaken vigorously (1250 rpm ...
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bioRxiv - Genomics 2019Quote: ... The library was then transformed into 10-beta electrocompetent cells (NEB C3020), grown in 100 mL of LB+Amp ...
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bioRxiv - Genomics 2020Quote: ... concentrated 4x and transformed in electrocompetent E.coli cells (10-beta Electrocompetent, NEB). Cells were recovered in SOC medium and plated on LB with Ampicillin ...
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bioRxiv - Molecular Biology 2022Quote: ... Correct constructs were re-transformed into 10-beta chemically competent cells (NEB), plated on LB agar plates containing chloramphenicol ...
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bioRxiv - Molecular Biology 2023Quote: ... and then transformed into NEB 10-beta competent cells (New England BioLabs). Plasmid DNA was extracted from ten positive clones using the GeneJET Plasmid Miniprep Kit (Thermo Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Correct constructs were re-transformed into 10-beta chemically competent cells (NEB), from which clones were used to prepare glycerol stocks (25% glycerol ...
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bioRxiv - Developmental Biology 2021Quote: ... and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli K-12 strain NEB DH-10 Beta (New England Biolabs, MA, USA) unless stated otherwise ...
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bioRxiv - Genomics 2020Quote: ... and the resulting product was transformed into 10-Beta Electrocompetent cells (NEB C3020K). We plated 1% of the library to estimate complexity and grew the rest of the sample and then midi prepped (Zymo D4200 ...
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bioRxiv - Biochemistry 2021Quote: ... as described by manufacturer and transformed into heat- competent 10-Beta cells (NEB). Inserts from single colonies were PCR amplified (using primers pGEM_T7_Fo ...
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bioRxiv - Molecular Biology 2021Quote: ... and transformed into NEB 10-beta electrocompetent E.coli (New England BioLabs Cat. #3020). DNA was extracted using a Qiagen Plasmid Midi Kit (Qiagen Cat ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The resulting plasmid lentiMPRA library was electroporated into 10-beta competent cells (NEB) using a Gemini X2 electroporation system (BTX ...
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bioRxiv - Microbiology 2022Quote: pWR1566 was purified from laboratory E.coli strain NEB 10-beta (New England Biolabs) and used to transform V.cholerae strain WR2700 using electroporation ...
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bioRxiv - Synthetic Biology 2022Quote: ... chemically competent DH5α cells or NEB 10-beta competent cells (NEB, Cat#C3019). Constructs were selected on LB plates (1% Tryptone ...
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bioRxiv - Genetics 2023Quote: ... and transformed into NEB 10-beta electrocompetent E.coli (New England BioLabs Cat. #3020). Plasmids were purified using a Qiagen Plasmid Midi Kit (Qiagen Cat ...
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bioRxiv - Systems Biology 2023Quote: ... The transformation into NEB 10-beta electrocompetent E.coli (New England Biolabs, Ipswitch, MA) was performed slightly differently ...
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bioRxiv - Systems Biology 2023Quote: ... Two transformations into NEB 10 beta electrocompetent cells (New England Biolabs, Ipswitch, MA) were performed as described above ...
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bioRxiv - Molecular Biology 2024Quote: ... and resuspending cells in 950 uL 10-beta/Stable Outgrowth Medium (NEB, B9035S). Serial dilutions of 10 uL were immediately spread onto Ampicillin selection plates to assay transformed library complexity (>= 10,000x) ...
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bioRxiv - Microbiology 2023Quote: ... The purified ligation reaction was transformed into NEB-10-Beta competent cells (NEB C3020K) and the lawns resulting from this transformation were pooled together and inoculated into LB broth supplemented with kanamycin ...
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bioRxiv - Microbiology 2023Quote: ... All four libraries were independently electroporated into NEB 10-beta electrocompetent cells (NEB, C3020K) and plated on LB agar supplemented with 15 μg/ml gentamicin at 37°C overnight.
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bioRxiv - Genomics 2024Quote: ... Ligation products were transformed into either 5-alpha or 10-beta electrocompetent cells (NEB) and grown in liquid LB-Amp cultures ...
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bioRxiv - Genomics 2024Quote: ... 975 μL of pre-warmed 10-beta/Stable Outgrowth Medium was added (NEB #B9035) to each cuvette ...
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bioRxiv - Molecular Biology 2020Quote: ... a mix of 4 μl First strand Synthesis Reaction Buffer (NEB-kit), 0.5 μl Murine RNase Inhibitor (NEB-kit ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were recovered in 1 ml NEB® 10-beta/Stable Outgrowth Medium (NEB, B9035) at 37 °C for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... transferred to 42°C and digested by immediate addition of 2ul of beta-agaraseI (NEB) per 300ul of melted gel and incubation at 42°C for 1h ...
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bioRxiv - Evolutionary Biology 2022Quote: ... transformations that involved the CRE library were performed using electroporation (NEB 10-beta electrocompetent E.coli), in all other cloning steps chemically competent E ...
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bioRxiv - Biochemistry 2024Quote: ... Purified products were again transformed into either NEB 10-beta (New England Biolabs, Ipswich, MA) or SIG10 (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2023Quote: ... according to manufacturer’s protocols and transforming chemically competent NEB 10-beta cells (NEB CN# C3019), where colony PCR and Sanger sequencing were again used to determine if the clone was correct.
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bioRxiv - Molecular Biology 2024Quote: ... The cosmid-I95 plasmid was amplified in a NEB 10-beta (New England Biolabs, C3019H), and the DNA was purified using a QIAfilter Plasmid Midi Kit (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were analysed by an MSD Elisa assay (Pacific Biolabs) using a CEP55 antibody (Novux ...
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bioRxiv - Synthetic Biology 2022Quote: ... and the ligation mix was directly used to transform 10-beta competent Escherichia coli cells (NEB), to amplify pSG01 ...
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bioRxiv - Molecular Biology 2024Quote: Escherichia coli strains used in this study were NEB 10-beta competent cells (New England Biolabs, Ipswich ...
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bioRxiv - Neuroscience 2022Quote: ... or the NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB) (replicates 4-5 and no salt treatment ...