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Citations for New England Biolabs :
51 - 100 of 10000+ citations for Mouse Lymphoid Enhancer Binding Factor 1 LEF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Factor Xa protease (New England Biolabs) was added to the MT solution at a molar ratio of 1 ...
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bioRxiv - Plant Biology 2021Quote: ... Factor Xa (P8010S, New England BioLabs) or TEV protease (P8112S ...
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bioRxiv - Biochemistry 2023Quote: ... 20 µg factor Xa protease (NEB) and 5 mM CaCl2 with inversion at 10 °C overnight ...
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bioRxiv - Developmental Biology 2019Quote: ... Subsequent pENTR clones were made by excising the original insert and replacing with new candidate enhancer fragments via Gibson Assembly (NEB HiFi Assembly Kit, New England Biolabs). The candidate enhancer fragments were shuttled to the transgenic vector pBPGUw [105] upstream of a minimal synthetic promoter sequence driving Gal4 using standard Gateway cloning techniques (LR Clonase ...
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bioRxiv - Neuroscience 2022Quote: ... was used to cut out Dlx enhancer and HBB promoter with MluI and EheI (NEB: R0606S).
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bioRxiv - Synthetic Biology 2021Quote: PCR amplicons corresponding to the enhancer segments were generated using Q5 polymerase (New England Biolabs M0492S), except for segment 2 which was generated with Kapa polymerase supplemented with 1 M Betaine ...
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bioRxiv - Genomics 2023Quote: ... Twist BioScience) and then assembled into pNL3.3 enhancer reporter plasmids using Gibson Assembly (New England Biolabs). Each cloned reporter plasmid was verified by Sanger sequencing (Quintara Bio) ...
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bioRxiv - Bioengineering 2023Quote: ... the AsCas12a-crRNA complexes were combined with 1 µL of binding buffer (New England BioLabs, #B6002S), 4.5 µL of biotinylated quencher probes ...
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bioRxiv - Cell Biology 2020Quote: ... The FER1 Ca2+-binding mutants in the C2D domain were generated by Q5 site directed mutagenesis kit (NEB) using primers 4833/4834 to change positions A1622 and A1634 to C resulting in Asp codon 542 and 545 changes to Ala.
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bioRxiv - Biochemistry 2024Quote: The ATP binding mutants (APLFD11A, APLFR37A, APLFK95A) were generated using Q5® Site- Directed Mutagenesis Kit (NEB #E0554) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Genomics 2021Quote: Putative enhancers were amplified from genomic DNA with Phusion High-Fidelity DNA Polymerase (New England Biolabs, # M0530). Betaine (1 M final concentration ...
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bioRxiv - Genomics 2021Quote: ... the CMV enhancer/promoter region was amplified from the pcDNA3.0 backbone using Phusion High-Fidelity Polymerase (NEB) and subcloned into the pUC19 vector upstream of the circEfnb2 or circMini backsplicing cassettes ...
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bioRxiv - Genomics 2022Quote: ... A first binding to streptavidin beads (NEB, S1421S) and washed as described (24) ...
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bioRxiv - Microbiology 2020Quote: Mutations within the stem-loop binding site of SHOxi were constructed using the Q5 Site-directed Mutagenesis kit (NEB) standard protocol on the previously described SHOxi overexpression construct (in pTA1300) ...
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bioRxiv - Cell Biology 2021Quote: All dox-inducible factors were generated by cloning the open reading frame of each factor into the pMINI vector (NEB) and then restricted with EcoRI or MfeI and inserted into the FUW-TetO expression vector ...
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bioRxiv - Immunology 2020Quote: ... GC-enhancer buffer and 0.25 μl of Q5® Hot Start High-Fidelity DNA Polymerase (NEB, Massachusetts, USA). Amplification was done after an initial phase of denaturation (95 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Mutation of the verified 7-mer binding site of the 3’-UTR of Zfp36l1 was performed using the Q5-site directed mutagenesis kit (New England BioLabs) using the following primers ...
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bioRxiv - Genetics 2023Quote: The site directed mutagenesis of the rbsD gene to introduce the stop codon and the mutated dsrA binding sites was carried out using the Q5 mutagenesis kit (New England Biolabs) and selection on kanamycin plates ...
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bioRxiv - Microbiology 2024Quote: To test the binding affinity of the TBT and TBTG mRNA to the 30S ribosomal subunit we used PURExpress ΔRibosome Kit (NEB) supplemented with 5 μM of 30S ribosomal subunit and 10 μM tRNAfMet in the presence of 1.4 μM of radioactively labelled mRNA (prepared as described above by in vitro translation followed by [32P] labelling as described for the northern blot probe labelling) ...
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bioRxiv - Microbiology 2020Quote: ... anti-maltose binding protein (MPB) (New England BioLabs #E8032L) and anti-outer membrane protein (OmpA ...
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bioRxiv - Genomics 2021Quote: ... RNA binding with streptavidin beads (NEB, Ipswich, MA; S1421) followed by RNA extraction with Trizol ...
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bioRxiv - Genomics 2022Quote: ... RNA binding with streptavidin beads (NEB, Ipswich, MA; S1421) followed by RNA extraction with Trizol ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse α-MBP (NEB, 1:10000); mouse α-Pol II CTD clone 8WG16 (Abcam ...
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bioRxiv - Biochemistry 2022Quote: In vitro protein translation was carried out (in absence of the release factors) using the PURExpress ΔRF123 kit (New England Biolabs). This guaranteed the stabilization of the ternary complexes mRNA-affitin-Ribosome ...
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bioRxiv - Genetics 2022Quote: Candidate enhancers were amplified from human genomic DNA using Q5 Hot-Start High-Fidelity DNA polymerase (New England Biolabs) and directionally cloned into the pGL3-Promoter plasmid (pGL3-P ...
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bioRxiv - Genomics 2020Quote: ... 0.5 μL of Ultra II Ligation Module Enhancer and 10 μL of Ultra II Ligation Module master mix (NEB) made up of a total of 20 μL with NFW ...
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bioRxiv - Genomics 2019Quote: Enhancer candidate regions were cloned into an Hsp68-promoter-LacZ reporter vector39 using Gibson cloning47 (New England Biolabs [NEB]). Transgenic mouse embryos were generated by pronuclear injection ...
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bioRxiv - Genomics 2019Quote: Enhancer candidate regions were cloned into an Hsp68-promoter-LacZ reporter vector39 using Gibson cloning47 (New England Biolabs [NEB]). Transgenic mouse embryos were generated by pronuclear injection ...
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bioRxiv - Neuroscience 2020Quote: We selected 17 sSNVs identified in brain-active enhancers and attempted cloning and site-directed mutagenesis (New England Biolabs) to recreate the mutations ...
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bioRxiv - Synthetic Biology 2023Quote: ... to generate the synthetic enhancer eGFP plasmid reporter (pTol2-synthetic enhancer-E1b-eGFP-Tol2) using HiFi DNA Assembly following manufacturer’s instructions (New England Biolabs). Reporter plasmid sequences were verified by Sanger sequencing ...
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bioRxiv - Cell Biology 2022Quote: ... Tracheal chitin was stained with 505 star conjugated chitin-binding probe (NEB, Frankfurt/M, Germany, used at 1:300). Nuclei were stained with 4’,6-Diamidino-2-Phenylindole ...
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bioRxiv - Cell Biology 2020Quote: Three point mutations in the predicted miR-145 seed binding site in DUSP6 were introduced in pGEM-T-DUSP6 3’UTR using a Phusion® site-directed mutagenesis kit (NEB) and the mutagenic primers ...
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bioRxiv - Neuroscience 2024Quote: ... C terminal and αPKC binding region) or PICK1 (BAR domain) were made with NEBuilder HiFi DNA Assembly Cloning Kit (NEB #E5520S). Mutations of PICK1 (KD-AA and 5K-E ...
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bioRxiv - Genomics 2020Quote: ... Nascent RNA was purified by binding streptavidin beads (NEB, S1421S) before and in between the following procedures ...
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bioRxiv - Biophysics 2020Quote: ... for the substrate-binding domain mutants and βamHI-AlwnI (NEB) for the transmembrane domain mutants (Appendix) ...
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bioRxiv - Genomics 2022Quote: ... Nascent RNA was purified by binding streptavidin beads (NEB S1421S) and washed as described by Mahat et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse monoclonal anti-MBP (1:2000, NEB), rabbit polyclonal anti-Spo11 (1:1000 ...
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bioRxiv - Biochemistry 2022Quote: ... MBP (mouse, 1:30,000, New England Biolabs), FLAG (mouse ...
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bioRxiv - Genetics 2021Quote: ... We then PCR amplified enhancers and promoters separately from the same array using Q5 high-fidelity DNA polymerase (NEB M0492). We amplified enhancers in four 50uL PCR reactions (98°C for 30 seconds ...
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bioRxiv - Molecular Biology 2020Quote: ... the SV40 promoter was removed from the luciferase-enhancer plasmids by restriction digestion with BglII and HindIII-HF (both NEB). The Fgf5 promoter region - encompassing the 300 bp region containing most of transcription initiation events in PRO-Cap-Seq data at the 5’ UTR of the gene plus 100 bp of flanking nucleotides on each side - was amplified by PCR and inserted in place of the SV40 promoter by Gibson Assembly ...
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bioRxiv - Plant Biology 2023Quote: ... and enhancer fragment combinations were cloned upstream of the 35S minimal promoter by Golden Gate cloning using BsaI-HFv2 (NEB). The resulting libraries were bottlenecked to yield 10–20 barcodes per enhancer.
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bioRxiv - Bioengineering 2024Quote: ... Linkers were then ligated to the DNA using the NEB Next Ultra II Ligation Master Mix and Ligation Enhancer (New England Biolabs). The CAST-seq PCR reactions were performed in forward and in reverse in respect to the cleavage site ...
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bioRxiv - Genetics 2024Quote: QF2-T2A-LexA-GAD ORF PCR-amplified with FRT-QF2-fwd + FRT-lexA-GAD-rev primer pair – used Hot-Start Q5 polymerase with GC enhancer (NEB);
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bioRxiv - Cell Biology 2020Quote: ... 3-4-points were induced in the miR-29a seed binding site using the Q5 Site Directed Mutagenesis Kit (NEB, Cat#E0554S). 20 ng of recombinant dual-luciferase plasmid and 6 pmol of either miR-29a mimic or scrambled control were mixed with 100 μL Opti-MEM containing 1 μL lipofectamine RNAiMax (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... of full-length SEC16A were generated by mutating 10 nucleotides in the silencer-select Sec16A siRNA (5’-CGGAGCUUCUGUUACGAGATT-3’, siRNA id: s19236, Thermo-Fischer Scientific) binding site using Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) following manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... for 2 hours and then crosslinked with p19 siRNA binding protein (1 μg/ml in depc-PBS; New England Biolabs) or anti-N6-methyladenosine (m6A ...
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bioRxiv - Microbiology 2020Quote: ... the resin was treated with factor Xa protease (P8010L, NEB) overnight at 4°C ...