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Citations for New England Biolabs :
51 - 100 of 4504 citations for Integrin beta 1 binding protein 1 ITGB1BP1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Strand extension was performed at 37°C for 10 minutes using 5uM of ssDNA binding protein (T4 Gene 32 Protein, NEB, Cat No. M0300S) and 0.8U/µL of Klenow Fragment (3’-5’ exo- ...
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bioRxiv - Cell Biology 2020Quote: ... Bacterial lysates were prepared and the fusion protein purified over a chitin binding column (NEB labs, UK). HA-Ub75-thioester was obtained by incubating the column material with mercaptosulfonate sodium salt (MESNa ...
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bioRxiv - Cancer Biology 2021Quote: ... Bacterial lysates were prepared and the fusion protein purified over a chitin binding column (NEB labs, UK). HA-Ub75- thioester was obtained by incubating the column material with mercaptosulfonate sodium salt (MESNa ...
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bioRxiv - Molecular Biology 2023Quote: ... Cascade complexes were purified via the N-terminal maltose binding protein (MBP) tag using amylose beads (NEB) and eluted with lysis buffer containing 10 mM maltose ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM MnCl2 only or together with 1 μl Lambda Protein Phosphatase (New England Biolabs). After incubation at 30°C for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... both proteins were deglycosylated by PNGase F (NEB, 1:50) overnight at 37°C in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... The mix consisted of 1 µL Cas9 protein (BioLabs, M0369M), 1 µL Fast Green FCF dye (Sigma ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... coli DH 10-beta (New England Biolabs) was transformed with Gibson assembly product ...
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bioRxiv - Developmental Biology 2022Quote: ... coli strain 10-beta (New England Biolabs) or TOP10 (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: ... Escherichia coli 10-beta (New England Biolabs) was used for plasmid construction ...
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bioRxiv - Bioengineering 2023Quote: ... NEB 10-beta (NEB cat. no. C3019), or NEB Stable (NEB cat ...
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bioRxiv - Microbiology 2023Quote: ... coli NEB beta cells (New England BioLabs) and verified by Sanger sequencing ...
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bioRxiv - Microbiology 2023Quote: ... coli NEB beta cells (New England BioLabs) and verified by Sanger sequencing ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli NEB10 beta was obtained from NEB. Cells are grown in LB media supplemented with Erythromycin (E6376 Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli 10-beta cells (New England Biolabs) with 5 μl of the assembly mix according to the protocol provided by the manufacturer ...
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bioRxiv - Plant Biology 2024Quote: ... coli cloning strain 10-beta (NEB # C3019H). E ...
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bioRxiv - Molecular Biology 2022Quote: ... and anti-MBP antibody (NEB E8032L; 1:10,000) in 1x TBST supplemented with 5% low-fat milk were incubated either at 4 °C overnight or 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... mouse antibodies against MBP (NEB E8032L, 1:20,000) were used to detect 2XMBP-tagged proteins and rabbit antibodies against HA (Cell Signaling C29F4 ...
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bioRxiv - Cell Biology 2023Quote: ... and 62.5 μL of Lambda Protein Phosphatase (New England Biolabs, 5 U μg-1 of protein) were added with gentle mixing after each addition ...
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bioRxiv - Genomics 2022Quote: The 2.2 kb DNA was ligated with 1 kb biotin- and dig-labeled DNA through SbfI- and XbaI-overhangs by T4 DNA ligase (NEB Biolabs). The ligated products were attached to the anti-dig treated surface of reaction chamber and streptavidin-coated magnetic beads ...
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bioRxiv - Molecular Biology 2023Quote: ... Fixed cells were resuspended in 1 mL spheroplast buffer (1.2 M sorbitol, 0.1 M potassium phosphate, 20 mM vanadyl ribonuclease complex [NEB S1402S], 20 µM beta-mercaptoethanol), and 1.2–5 µL 100T zymolyase (US Biological Z1005 ...
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bioRxiv - Cell Biology 2024Quote: ... Every 2 PCR reactions were pooled and purified in 100ul elution buffer using Monarch PCR & DNA Cleanup Kit (7:1 binding buffer, NEB#T1030L). 5ul/reaction Dynabeads M270 Streptavidin (Thermofisher#65305 ...
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bioRxiv - Microbiology 2022Quote: ... 1-20 μg of the Env protein was mixed with 1 μl of Glycoprotein Denaturing Buffer (NEB, 10×) and H2O (if necessary ...
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bioRxiv - Molecular Biology 2024Quote: ... MBP tagged protein was subsequently bound in batch for 1 h to 1 ml of Amylose resin (NEB) per litre of input culture (typically 4 ml total resin) ...
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bioRxiv - Neuroscience 2021Quote: ... 1 µl of lambda protein phosphatase (New England BioLabs, Ipswich, MA). Each mixture was incubated at 30 °C for either 1 ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 μl of Lambda protein phosphatase (P0753L, New England BioLabs) were incubated with the proteins at 30°C for 30 minutes.
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bioRxiv - Genetics 2024Quote: ... 1 µL (FC 1.67 µM) Cas9 protein (New England Biolabs, Cat.M0646) was freshly prepared on the morning of the injection procedure ...
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bioRxiv - Genomics 2022Quote: The 2.2 kb DNA was ligated with 1 kb biotin- and dig-labeled DNA through SbfI- and XbaI-overhangs by T4 DNA ligase (NEB Biolabs). The ligated products were attached to the anti-dig treated surface of reaction chamber and streptavidin-coated magnetic beads ...
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bioRxiv - Molecular Biology 2024Quote: ... and the biotin-dNTPs were removed from unligated ends by resuspending the pellet in 100 μl of 1X NEB buffer #1 (NEB B7001S) and 5 U/μl exonuclease III (NEB M0206L ...
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bioRxiv - Biophysics 2024Quote: ... We mixed the digested handles with the large 38 kb fragment in 10:1 molar ratio (biotin-handles to DNAparS) and added T4 DNA ligase (New England Biolabs, M0202L) and 1 mM ATP for ligation ...
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli NEB 10-beta (New England Biolabs) was used for plasmid preparation and grown in medium supplemented as instructed by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid was transformed into 10-beta cells (NEB) and clone sequence verified by sanger sequencing.
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bioRxiv - Biochemistry 2022Quote: ... coli NEB 10-beta cells (New England Biolabs) and plated on selective LB agar plates (50 µg/mL kanamycin ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli 10-beta was purchased from NEB (C3020). E ...
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bioRxiv - Molecular Biology 2023Quote: ... coli strain NEB 10-beta (NEB Cat# C3019) was used for construction of plasmids by ligation-less Gibson assembly (Fu et al ...
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bioRxiv - Synthetic Biology 2023Quote: ... into 100 µL 10-beta electrocompetent cells (NEB), and then plated on 10 15 cm LBSpect agar plates for 16-20 h (37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... coli strain NEB 10-beta (New England Biolabs) was used for all cloning ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli (NEB 10-beta, New England Biolabs C3020K), and plated at limiting dilutions on LB+ampicillin plates ...
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bioRxiv - Molecular Biology 2023Quote: ... coli NEB 10-beta cells (New England Biolabs) and all constructs were sequence verified using plasmidsaurus Oxford Nanopore long read sequencing ...
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bioRxiv - Microbiology 2023Quote: ... coli strain D10-beta (New England Biolabs Inc.) was used in the entire cloning process ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli (NEB 10-beta, New England Biolabs C3020K), and plated at limiting dilutions on LB+ampicillin plates ...
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bioRxiv - Microbiology 2022Quote: ... coli (NEB 10-beta, New England Biolabs C3020K), and plated at limiting dilutions on LB+ampicillin plates ...
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bioRxiv - Microbiology 2022Quote: ... coli (NEB 10-beta, New England Biolabs C3020K), and plated at limiting dilutions on LB+ampicillin plates ...
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bioRxiv - Cell Biology 2023Quote: ... 2 U of beta-agarose I (NEB, M0392), and 5 μL of Maxima H minus reverse transcriptase ...
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bioRxiv - Microbiology 2024Quote: ... coli (NEB 10-beta, New England Biolabs C3020K), and plated at limiting dilutions on LB+ampicillin plates ...
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bioRxiv - Molecular Biology 2024Quote: ... coli strain NEB10-beta (New England Biolabs #C3019H) grown in LB-medium (Sigma-Aldrich #L3022 ...
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bioRxiv - Microbiology 2024Quote: ... coli (NEB 10-beta, New England Biolabs C3020K), and plated at limiting dilutions on LB+ampicillin plates ...
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bioRxiv - Bioengineering 2024Quote: ... coli NEB 10-beta cells (NEB, cat# C3019H) were used for plasmid construction ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary mouse antibodies against MBP (NEB E8032L, 1:5000) and RAD51 (Novus Biologicals 14b4 ...
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bioRxiv - Neuroscience 2022Quote: ... and probed with p62 antibody (NEB D5L7G, 1:800) and streptavidin-594 (Biolegend 405240 ...