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Citations for New England Biolabs :
51 - 100 of 344 citations for Human Maspin Highly Pure Recombinant since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... MEFs were protein transfected with recombinant RNase H (NEB) using Project Reagent Transfection Kit (Thermo ...
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bioRxiv - Molecular Biology 2023Quote: ... a combination of both recombinant RBPMS and BSA (NEB). Splicing reactions were incubated for the 3 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... 20pmol of recombinant histone H3.1-H4 tetramer (NEB, M2509S) were immediately added to the tube ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 μL recombinant shrimp alkaline phosphatase (NEB cat#M0371S) was added and incubated at 37°C for 2 hrs ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μL recombinant shrimp alkaline phosphatase (NEB cat#M0371S) was added to the reaction and incubated at 37°C for 1hr ...
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bioRxiv - Genomics 2024Quote: ... (b) PURExpress T7 recombinant IVT system (NEB Cat.#E6800L), for inserts in pTH6838 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 100 μg/ml Recombinant Albumin) (#B6003V, New England Biolabs) at a final volume of 30 μL ...
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bioRxiv - Microbiology 2020Quote: ... Extracts were diluted to 1μg pure nucleotide concentration and purified by DNase treatment (New England Biolabs). cDNA was synthesized from RNA by using the iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... diluted in pure water and quantified with the Luna® Universal qPCR Master mix from NEB as previously described.
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bioRxiv - Biophysics 2024Quote: ... If the PCR product was pure then the sample was purified (NEB DNA Clean-up kit). 100 ng of purified DNA was used as a template for in vitro transcription (NEB E2040S ...
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bioRxiv - Immunology 2021Quote: Purified recombinant CV3-25 IgG was mixed with LysC (NEB) at a ratio of 1μg LysC per 10mg of IgG and incubated at 37°C for 18h with nutation ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant kinases (2500 U/mg protein for PKA (NEB-P600S), 500:1 Tau:kinase for Gsk3ß (BPS-40007) ...
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bioRxiv - Cell Biology 2022Quote: ... purified recombinant PKA proteins (2,500 units/ml, New England Biolabs) along with 100 μM cAMP ...
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bioRxiv - Developmental Biology 2020Quote: ... Kinase assays were performed using recombinant murine c-Abl (NEB). The amounts of GST-fusion protein to add to the reaction were calibrated using western blots of the purification products with mouse anti-NICD (1:1000 ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant hACE2 protein was digested using EndoH (New England Biolabs) and thrombin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... PCR-amplified recombinant DNA was treated with DpnI (NEB Biolabs) for 3 h ...
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bioRxiv - Cell Biology 2022Quote: ... PCR-amplified recombinant DNA was treated with DpnI (NEB Biolabs) for 3 h ...
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bioRxiv - Immunology 2022Quote: ... recombinant proteins were treated with PNGase F (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: Recombinant RfxCas13d proteins were expressed in E.coli NiCo21(DE3) (NEB C2529). Cells were grown in 1L of lysogeny (Luria-Bertrani ...
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bioRxiv - Immunology 2020Quote: ... and dephosphorylated using recombinant shrimp alkaline phosphatase (New England BioLabs M0371L). Annealed and phosphorylated oligonucleotides were cloned into linearized and dephosphorylated BPK1520_puroR using T4 DNA Ligase (New England BioLabs M0202L ...
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bioRxiv - Immunology 2021Quote: ... A similar dilution series of recombinant histone H3 (New England Biolabs) was prepared starting at 1 µg/ml protein ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 mM DTT with 1.5 µM recombinant H3 (New England Biolabs), vehicle or 500 nM recombinant enzyme ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were induced in SHuffle Express strains (New England Biolabs) upon addition of 1mM IPTG in cultures containing kanamycin 50 μg/mL and incubated with agitation at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Protease Inhibitors) with recombinant PKA kinase (2500 U/mg, NEB-P600S) and 1 mM ATP overnight at 30°C and 250 rpm ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant bacmids were created by transformation into DH10Bac competent cells (NEB), screening on XGAL plates ...
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bioRxiv - Molecular Biology 2020Quote: ... Constructs for translation in the PURE system were generated from the T7 PURExpress plasmid (New England Biolabs, USA). The coding sequence of full length human WNK1 was derived from a commercial expression vector (#RC218208 ...
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bioRxiv - Biophysics 2024Quote: ... A 4°C AKTA Pure FPLC system (Cytiva) was prepared with a 10 mL amylose column (NEB #E8021S) and 2 mL/min flow rate ...
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bioRxiv - Neuroscience 2022Quote: ... the recombinant proteins were purified by amylose resin (#E8022S, New England Biolabs) according the manual instructions ...
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bioRxiv - Synthetic Biology 2021Quote: The recombinant CBX8-CD was expressed in BL21 (DE3) (New England Biolabs) Escherichia coli cells ...
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bioRxiv - Biophysics 2021Quote: ... Recombinant M-MuLV reverse transcriptase from the ProtoScript® II kit (NEB) was used to synthesize first strand cDNA from the annealed primer-MS2 RNA mix ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GFP-fusion proteins were purified from BL21(DE3) cells (NEB, # C2527H) using an N-terminal H6-tag ...
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bioRxiv - Biochemistry 2022Quote: The recombinant Lili-Mips were treated with PNGase F (New England Biolabs) to remove the N-linked oligosaccharides ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant vectors were generated using HiFi DNA Assembly Cloning Kit (NEB E5520). During cloning procedures ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant vectors were constructed using HiFi DNA Assembly Cloning Kit (NEB E5520). electroporation (device) ...
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bioRxiv - Biochemistry 2024Quote: 15 μg of recombinant LyLAP was deglycosylated using PNGase F (NEB, P0704) in either native or denaturing conditions ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR amplification was performed using recombinant Q5 DNA polymerase (New England Biolabs) and primers listed in Table S1 ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 nM Ca2+ and 10 μM purified Calmodulin (CaM) in the coupled transcription/translation PURE system (New England Biolabs, USA), which contains a defined set of purified translation factors and E ...
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bioRxiv - Synthetic Biology 2024Quote: ... 10 µL of the reaction mixture was used to purify the proteins contained in the PURE system using a His-tag spin column (New England Biolabs), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were affinity-purified using amylose resin using the manufacturer’s protocol (NEB) and eluted with 20 mM maltose ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM CaCl2) at room temperature in the presence of recombinant enteropeptidase (NEB) at 13 U enzyme per mg TMPRSS2 zymogen ...
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bioRxiv - Molecular Biology 2021Quote: ... The recombinant protein was sequentially purified using an amylose column (New England Biolabs) followed by a nickel-nitrilotriacetic acid column (Qiagen ...
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bioRxiv - Genomics 2024Quote: ... 200 µg/mL molecular biology grade recombinant albumin (rAlbumin) (New England Biolabs B9200S), 0.8 U/µL RiboLock RNase inhibitor (Thermo Fisher Scientific EO0384) ...
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bioRxiv - Plant Biology 2024Quote: ... 2µg of recombinant protein was mixed with 400U lambda protein phosphatase (NEB, #P0753S) at 30°C for 30 min before separated by an SDS-PAGE ...
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bioRxiv - Immunology 2024Quote: ... 1 µl of the recombinants was transformed into NEBstable competent cells (NEB, USA). Bacterial cells were spread on agarose plates ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM MgCl2 and 2 U of recombinant Escherichia coli RNase HI (NEB) were added ...
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bioRxiv - Biophysics 2020Quote: The cell-free transcription/translation mix used in this study is a customized version of the PURE system (40) without ribosomes (PUREexpress Δ ribosomes, New England Biolabs) and the amino acid histidine ...
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bioRxiv - Synthetic Biology 2022Quote: ... and eluted in 10 μL of Ultra Pure™ DNase/RNase free distilled water: the purified amplicon and the 1kb DNA ladder (NEB) were run on 1% agarose gel in 1x TBE for 1 h 30 min at 110 V ...
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bioRxiv - Synthetic Biology 2022Quote: ... and eluted in 10 μL of Ultra Pure™ DNase/RNase free distilled water: the purified amplicon and the low molecular weight DNA ladder (NEB) were run on 2% agarose gel in 1x TBE for 1 h 40 min at 100 V ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Placenta (M0307, New England Biolabs) and 25 U of M-MuLV Reverse Transcriptase with corresponding buffer (M0253 ...
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bioRxiv - Systems Biology 2024Quote: ... human (E6320, New England Biolabs, USA) and sequenced on a MiSeq (Illumina ...