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Citations for New England Biolabs :
51 - 100 of 3347 citations for Ephrin type B receptor 1 EphB1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Cell pellets were resuspended in 1 ml of iCLIP lysis buffer B (same as buffer A but with 1% v/v NP-40 and 11 μl of Murine RNase inhibitor (NEB) per 1 ml ...
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bioRxiv - Synthetic Biology 2020Quote: ... Type IIS assembly with BsmbI (New England Biolabs, MA, USA) and annealed oligo cloning ...
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bioRxiv - Developmental Biology 2024Quote: ... The genomic region around rbpms2aae30 was amplified for 35 cycles with an annealing temperature of 60°C and the wild-type allele was digested with HaeIII for 1 hour (New England Biolabs, R0108S)2 ...
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bioRxiv - Developmental Biology 2024Quote: ... The surrounding tsc2vu242 genomic region was amplified for 35 cycles with an annealing temperature of 57°C and the wild-type allele was digested with HpyCH4IV for 1 hour (New England Biolabs, R0169S). Undigested and digested products were resolved in a 3% Metaphor 1:1 (Lonza)/agarose gel (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmids encoding chimeric SCS6/MLA1 and SCS6/MLA6 receptors were assembled using the NEBuilder HiFi assembly Kit (NEB) based on the domain boundaries reported in (28) ...
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bioRxiv - Microbiology 2020Quote: The plasmid pAAV S1-Fc was digested with New England Biolabs (NEB) Restriction Enzymes Pvu I-HF (Cat ...
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bioRxiv - Biochemistry 2021Quote: ... 125 to 75 U/ 5 μg RNase B (Cat.P7817S, NEB) and fetuin (Cat ...
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bioRxiv - Molecular Biology 2020Quote: ... Cas9 protein (IDT) was suspended with a Diluent B (NEB) to make 1 μM solution ...
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bioRxiv - Synthetic Biology 2022Quote: ... and sRNA plasmids using BsaI Type IIS Enzyme (New England Biolabs) and transformed into E ...
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bioRxiv - Bioengineering 2023Quote: ... Remnant wild-type DNA was degraded via DPN1 (New England Biolabs) digestion for two hours at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... RNase B from bovine pancreas (New England Biolabs, Ipswich MA, USA), and horse radish peroxidase (HRP ...
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bioRxiv - Synthetic Biology 2023Quote: ... the PURExpress® kit containing Solution A and Solution B (NEB) was used to prepare the transcription-translation reaction ...
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bioRxiv - Microbiology 2024Quote: ... coli B strain was dephosphorylated with 0.4 U/µL QuickCIP (NEB) in 1 x rCutSmart buffer (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... Golden Gate Assembly protocol was applied with type IIS endonuclease Esp3I (NEB). The product was transformed into competent E ...
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bioRxiv - Immunology 2023Quote: ... standard restriction cloning using the Type IIS restriction enzyme BsmBI-v2 (NEB) was employed to insert the leader peptides and variable regions into pVITRO1 (mouse IgG1 κ) ...
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bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...
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bioRxiv - Systems Biology 2024Quote: ... human (E6320, New England Biolabs, USA) and sequenced on a MiSeq (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Placenta (M0307, New England Biolabs) and 25 U of M-MuLV Reverse Transcriptase with corresponding buffer (M0253 ...
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bioRxiv - Genetics 2019Quote: ... The 3′-UTR sequences of both genes were then amplified from genomic DNA obtained from HEK293 cells with Phusion® High-Fidelity DNA Polymerases (NEB, Ipswich, MA) and cloned into the multiple cloning site of the pMIR-REPORT luciferase miRNA expression vector (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: The wild-type fragments were amplified with Phusion High-Fidelity DNA Polymerase (NEB) using HEK293T cDNA ...
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bioRxiv - Cell Biology 2019Quote: Purified Wss1 wild type (3.1 μM) was mixed with purified histone H3 (NEB) (3.3 μM ...
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bioRxiv - Synthetic Biology 2022Quote: ... 10,000 units Type IIs restriction enzymes (T7 DNA ligase, Esp31/BsaI-v2, NEB), and 1 μL T4 DNA ligase (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... Freshly laid wild-type TU-strain embryos were injected with SpCas9 protein (NEB) mixed with gRNAs (~300 ng/ul) ...
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bioRxiv - Synthetic Biology 2023Quote: ... In the second Type IIS DNA assembly reaction using BbsI (New England Biolabs), these transcriptional unit constructs were assembled into the backbone plasmid pML3001 (Lebovich and Andrews 2022 ...
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bioRxiv - Genetics 2019Quote: ... The ligation reaction mixture B consisted of appropriate amount of T3 ligase (NEB) with ligation buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Cas9 was diluted to 7 μM with diluent buffer B (NEB, Ipswich USA) on arrival and stored at −20 °C ...
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bioRxiv - Cancer Biology 2019Quote: Purified hTERT_191-306 proteins were incubated with CDK1-cyclin B (New England Biolabs) or purified IKK2_2-664 proteins ...
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bioRxiv - Cell Biology 2022Quote: ... B and C oligonucleotides were phosphorylated with T4 polynucleotide kinase (NEB, Cat #M0201L) according to the manufacturer recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... elegans osgn-1 (F30B5.4a, WormBase WS265) and human OSGIN1 were amplified by PCR using Phusion DNA polymerase (NEB, M0530L), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Molecular Biology 2021Quote: pUC19 was digested using thirteen commercially available Type II restriction enzymes (New England Biolabs). For BamHI ...
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bioRxiv - Bioengineering 2022Quote: ... The template DNA was removed by using DpnI type IIM restriction enzyme (NEB, USA) at 37°C (1 – 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... and/or 10 U of T4 PNK (wild-type or 3’ phosphatase minus, NEB). For RtcB ligation ...
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bioRxiv - Biophysics 2022Quote: ... type IIS enzymes (BsaI, New England Biolabs #R3733, and BsmBI, New England Biolabs #R0739) were used to iteratively concatenate sequence modules ...
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bioRxiv - Developmental Biology 2022Quote: ... along with enzymatic digestion of the wild-type amplicon with enzyme BslI (NEB R0555S). Genotyping dmrt2bumb4required treatment with the enzyme BaeGI (NEB R0708S) ...
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bioRxiv - Biophysics 2023Quote: ... the use of a Type IIs restriction enzyme (BsaI-HF-v2, New England Biolabs) that produces unique 4 bp sticky ends ensures that the three fragments are oriented in a unique way.
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bioRxiv - Microbiology 2023Quote: ... Genes of interest were amplified from wild-type genomic DNA using Phusion Polymerase (NEB), according to the manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2024Quote: ... R0148S)2 or the wild-type allele was digested with HphI (New England Biolabs, R0158S) for 1 hour ...
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bioRxiv - Microbiology 2019Quote: ... falciparum and human histones (New England Biolabs) (6µg) ...
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bioRxiv - Microbiology 2021Quote: ... 40 U RNase Inhibitor Human Placenta (NEB), 50 pmol Oligo d(T)23 (NEB ...
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bioRxiv - Biochemistry 2019Quote: ... Human CK2 was obtained from NEB (P6010S). IE2-NTD was phosphorylated in buffer provided by the manufacturer which contains 50mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: Recombinant human Casein Kinase 2 (NEB, P6010S) and Protein Kinase A (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... A fragment of human Plexin-B1 cDNA (encoding amino acids 1-535) was cloned into the pcDNA5 vector using HindIII (R0104S, NEB) and XhoI (R0146S ...
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bioRxiv - Biochemistry 2021Quote: ... per 1g of wet cell weight and 1 Unit of 30 Units/μL RNase Inhibitor (from human placenta; New England Biolabs (NEB), No ...
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bioRxiv - Microbiology 2022Quote: ... Institut Jacques Boy), recombinant human IL-2 (rhIL2, 10 U/ml, Miltenyi) and DNase (1 U/ml, New England Biolabs), washed and enumerated ...
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bioRxiv - Cell Biology 2023Quote: ... The pet30-B vector was digested with Nde1 (R0111, New England BioLabs, Waltham, MA) and EcoRI-HF (R3101 New England BioLabs ...
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bioRxiv - Biochemistry 2019Quote: ... This assembly was performed using a Type IIs restriction enzyme BsaI (New England Biolabs # R3535) and T4 DNA ligase (New England Biolabs # M0202 ...
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bioRxiv - Genomics 2020Quote: KlenTaq wild type gene was amplified using a high fidelity PCR (Q5 polymerase from NEB) from a stored plasmid ...
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bioRxiv - Biochemistry 2022Quote: ... wild-type CSF3R MIG plasmid and insert were digested with SfiI (New England Biolabs R0123S), ligated overnight with a 3:1 molar ratio ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein coding sequences were ordered synthetically with Part 3b type BsaI overhangs (NEB R3733) and cloned into the entry vector with BsmBI (NEB R0739) ...