Labshake search
Citations for New England Biolabs :
51 - 100 of 180 citations for Aprepitant Beta Glucuronide Sodium Salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2023Quote: ... coli NEB 10-beta from New England Biolabs (NEB) was used for sensor assays ...
-
bioRxiv - Cell Biology 2024Quote: ... Assemblies were transformed into 10-beta competent cells (NEB) and plated onto LB agar plates for overnight incubation at 37°C ...
-
bioRxiv - Synthetic Biology 2024Quote: ... transformed into NEB 10-beta electrocompetent cells (NEB C3020K), and extracted using the Qiagen Plasmid Maxi Kit (Qiagen 12162) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... or electroporation (NEB 10-beta, Cat# C3019H, 1.8 kV) with 50 ng of plasmid DNA ...
-
bioRxiv - Microbiology 2021Quote: ... coli (NEB 10-beta electrocompetent cells, New England BioLabs C3020K) and plated at a target bottleneck of 50,000 variants per duplicate library ...
-
bioRxiv - Genomics 2022Quote: ... The product was transformed into 10-beta Electrocompetent E.coli (NEB), by electroporation with 2.0kV ...
-
bioRxiv - Microbiology 2022Quote: ... coli (NEB 10-beta electrocompetent cells, New England BioLabs C3020K) and plated at a target bottleneck of 100,000 variants per duplicate library ...
-
bioRxiv - Immunology 2022Quote: ... coli (NEB 10-beta electrocompetent cells, New England BioLabs #C3020K), and bottlenecked to ∼1 × 105 cfus (an average of >25 barcodes per single-mutant) ...
-
bioRxiv - Immunology 2024Quote: ... we electroporated 100 µl of NEB 10-beta (NEB, #C3020K) in a BioRad electroporator using the preset E ...
-
bioRxiv - Synthetic Biology 2024Quote: ... commercial 10-beta competent cells (New England Biolabs, Ipswitch, MA) were transformed with plasmids carrying a canvas repeat sequence-targeting sgRNA cassette driven by strong constitutive promoter apFAB36 [21] ...
-
bioRxiv - Genomics 2023Quote: ... followed by transformation into 10-beta competent cells (NEB, C3020) using the Gemini X2 machine (BTX) ...
-
bioRxiv - Genetics 2024Quote: ... and then transformed into 10-beta competent cells (NEB, C3020) via electroporation ...
-
bioRxiv - Genetics 2020Quote: ... sodium orthovanadate (NEB, 1mM), and sodium fluoride (NEB ...
-
bioRxiv - Physiology 2022Quote: ... sodium orthovanadate (NEB, 1mM), sodium fluoride (NEB ...
-
bioRxiv - Developmental Biology 2020Quote: ... and transformed into NEB 10-beta Competent E.coli (NEB, Ipswich, MA). Colonies positive for the Lgals3-R200S allele by PCR were grown overnight at 37 °C with nutation at 200 rpm ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... coli K-12 DH10B (Neb 10-beta, NEB catalogue no. C3019H), E ...
-
bioRxiv - Microbiology 2020Quote: ... and the assembly reaction used to transform E.coli (NEB 10 beta). Colonies positive by PCR screening using primers that flank the cloning site were sequenced across the entire S coding region and a single positive isolate adopted for all further manipulations.
-
bioRxiv - Microbiology 2021Quote: Escherichia coli (NEB® 10-beta Electrocompetent E. coli or NEB® 5-alpha Competent E ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and 400 U of Salt-T4 DNA Ligase (NEB). All reactions were then incubated at 37 °C 30 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... 8 U/μl Salt-T4 DNA ligase (NEB #M0467)) ...
-
bioRxiv - Physiology 2022Quote: ... sodium fluoride (NEB, 1 mM), and sodium butyrate (100uM) ...
-
bioRxiv - Biophysics 2021Quote: ... the library was transformed into elec-trocompetent NEB 10-beta cells (NEB) to yield ¿1000x transformants per gene ...
-
bioRxiv - Synthetic Biology 2021Quote: ... NEB 10-beta/Stable Outgrowth Medium (450 μL; B9035S, New England Biolabs) was added to each aliquot and the mixture was shaken vigorously (1250 rpm ...
-
bioRxiv - Genomics 2020Quote: ... concentrated 4x and transformed in electrocompetent E.coli cells (10-beta Electrocompetent, NEB). Cells were recovered in SOC medium and plated on LB with Ampicillin ...
-
bioRxiv - Molecular Biology 2022Quote: ... Correct constructs were re-transformed into 10-beta chemically competent cells (NEB), plated on LB agar plates containing chloramphenicol ...
-
bioRxiv - Biochemistry 2024Quote: ... Correct constructs were re-transformed into 10-beta chemically competent cells (NEB), from which clones were used to prepare glycerol stocks (25% glycerol ...
-
bioRxiv - Molecular Biology 2023Quote: ... and then transformed into NEB 10-beta competent cells (New England BioLabs). Plasmid DNA was extracted from ten positive clones using the GeneJET Plasmid Miniprep Kit (Thermo Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... All proteins were expressed in NEB 10-beta cells (New England Biolabs). Cells transformed with the corresponding expression vector were grown in LB medium supplemented with 100 µg/ml Ampicillin at 37 °C to an OD600 of 0.8 ...
-
bioRxiv - Synthetic Biology 2021Quote: ... coli K-12 strain NEB DH-10 Beta (New England Biolabs, MA, USA) unless stated otherwise ...
-
bioRxiv - Genomics 2020Quote: ... and the resulting product was transformed into 10-Beta Electrocompetent cells (NEB C3020K). We plated 1% of the library to estimate complexity and grew the rest of the sample and then midi prepped (Zymo D4200 ...
-
bioRxiv - Biochemistry 2021Quote: ... as described by manufacturer and transformed into heat- competent 10-Beta cells (NEB). Inserts from single colonies were PCR amplified (using primers pGEM_T7_Fo ...
-
bioRxiv - Molecular Biology 2021Quote: ... and transformed into NEB 10-beta electrocompetent E.coli (New England BioLabs Cat. #3020). DNA was extracted using a Qiagen Plasmid Midi Kit (Qiagen Cat ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The resulting plasmid lentiMPRA library was electroporated into 10-beta competent cells (NEB) using a Gemini X2 electroporation system (BTX ...
-
bioRxiv - Microbiology 2022Quote: pWR1566 was purified from laboratory E.coli strain NEB 10-beta (New England Biolabs) and used to transform V.cholerae strain WR2700 using electroporation ...
-
bioRxiv - Synthetic Biology 2022Quote: ... chemically competent DH5α cells or NEB 10-beta competent cells (NEB, Cat#C3019). Constructs were selected on LB plates (1% Tryptone ...
-
bioRxiv - Molecular Biology 2024Quote: ... and resuspending cells in 950 uL 10-beta/Stable Outgrowth Medium (NEB, B9035S). Serial dilutions of 10 uL were immediately spread onto Ampicillin selection plates to assay transformed library complexity (>= 10,000x) ...
-
bioRxiv - Genetics 2023Quote: ... and transformed into NEB 10-beta electrocompetent E.coli (New England BioLabs Cat. #3020). Plasmids were purified using a Qiagen Plasmid Midi Kit (Qiagen Cat ...
-
bioRxiv - Systems Biology 2023Quote: ... The transformation into NEB 10-beta electrocompetent E.coli (New England Biolabs, Ipswitch, MA) was performed slightly differently ...
-
bioRxiv - Systems Biology 2023Quote: ... Two transformations into NEB 10 beta electrocompetent cells (New England Biolabs, Ipswitch, MA) were performed as described above ...
-
bioRxiv - Cancer Biology 2024Quote: ... ligated vectors were then transformed into NEB10-beta electroporation ultracompetent E.coli cells (NEB® 10-beta Electrocompetent E ...
-
bioRxiv - Biochemistry 2024Quote: ... The ligation mix were used to transform chemical-competent NEB10-beta (NEB #C3019) or electro-competent XL1-Blue ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2 mM Vanadate (Sodium Orthovanadate, NEB, pre-incubated for ten minutes at 95 °C to dissociate Vanadate oligomers ...
-
bioRxiv - Genetics 2020Quote: ... and sodium fluoride (NEB, 1 mM). 150uL of ice cold buffer was added to heads followed by immediate homogenization with a motorized pestle for 10 seconds on ice ...
-
bioRxiv - Microbiology 2023Quote: ... The purified ligation reaction was transformed into NEB-10-Beta competent cells (NEB C3020K) and the lawns resulting from this transformation were pooled together and inoculated into LB broth supplemented with kanamycin ...
-
bioRxiv - Genomics 2024Quote: ... Ligation products were transformed into either 5-alpha or 10-beta electrocompetent cells (NEB) and grown in liquid LB-Amp cultures ...
-
bioRxiv - Genomics 2024Quote: ... 975 μL of pre-warmed 10-beta/Stable Outgrowth Medium was added (NEB #B9035) to each cuvette ...
-
bioRxiv - Microbiology 2023Quote: ... All four libraries were independently electroporated into NEB 10-beta electrocompetent cells (NEB, C3020K) and plated on LB agar supplemented with 15 μg/ml gentamicin at 37°C overnight.
-
bioRxiv - Cell Biology 2020Quote: ... or sodium orthovanadate (Na3VO4, 1 mM; NEB) to test dephosphorylation of CatSper1 ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... 5 mM sodium orthovanadate (New England Biolabs), and 10 mM sodium fluoride (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2023Quote: ... containing 1 mM sodium orthovanadate (NEB, # P0758S). The membrane from the transwell inserts was cut using forceps and placed in 300 µL of 1X cell lysis buffer (Invitrogen ...