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Citations for New England Biolabs :
901 - 950 of 10000+ citations for rno mir 101a Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The cDNAs were synthesized with 10U of AMV RT (NEB) for 20 min at 42°C ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was generated from RNA by LunaScript RT SuperMix (NEB). PCR reactions targeting the 3CLpro gene were performed using Q5® High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... primed by primer SR-RT (NEB, sequence see Table S4) complementary to the 3’ DNA adaptor ...
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bioRxiv - Developmental Biology 2020Quote: ... were used for reverse transcription using ProtoSciprt II RT (NEB). Synthesized cDNA was diluted five times in water ...
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bioRxiv - Microbiology 2023Quote: ... 2ug RNA was reverse-transcribed using Lunascript RT (NEB E3010L), following the manufacturers protocol ...
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bioRxiv - Microbiology 2023Quote: ... A single RT-LAMP reaction consisted of enzymes (RTx (NEB), Bst 2.0 (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1× ProtoScript II RT Reaction Buffer (New England Biolabs) was incubated for 1 h at 30°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 μl 10× Template Switching RT Enzyme Mix (NEB) were added to the mixture and the reaction was incubated at 42 °C for 90 min followed by 85 °C for 5 min ...
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bioRxiv - Plant Biology 2020Quote: ... The amplified NbPRS4 PCR product was gel purified (Monarch DNA Gel Extraction Kit, New England Biolabs), digested with XbaI and XhoI (New England Biolabs) ...
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bioRxiv - Developmental Biology 2021Quote: The resulting PCR fragments were gel purified using the Monarch Gel Purification Kit (New England Biolabs) and TA cloned into pGEM-T Easy (Promega ...
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bioRxiv - Genetics 2022Quote: ... Sequencing libraries were prepared using the NEBNext Ultra™ II PCR-free kit (New England Biolabs). In short ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were gel purified with Monarch® DNA Gel Extraction Kit (T1020S, New England BioLabs) according to manufacturer’s instructions and sent for NGS at the Gurdon Institute NGS core using an Illumina HiSeq 1500.
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bioRxiv - Microbiology 2019Quote: ... The PCR products were then individually ligated using a KLD Enzyme reaction kit (New England Biolabs) for 3 hours and subsequently transformed into ElectroMAX DH10B E ...
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bioRxiv - Zoology 2020Quote: ... we prepared PCR-free libraries using NEBNext Ultra II DNA library prep kit (New England Biolabs), but with TruSeq DNA Single Indexes (Set B ...
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bioRxiv - Molecular Biology 2019Quote: ... Associated DNA was then purified by extraction using Monarch PCR & DNA Cleanup Kit (New England Biolabs). Immunoprecipitated DNA and total input were used as a template for real-time qPCR ...
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bioRxiv - Synthetic Biology 2019Quote: ... and subsequently cloned in to the pMini-T plasmid using the PCR Cloning Kit (NEB #E1202). Plasmids were extracted from E.coli JM109 transformed cells using the QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Cancer Biology 2019Quote: ... After determining accurate concentrations by quantitative PCR using the NEBnext library quant kit for Illumina (NEB), pooled libraries were sequenced on an Illumina HiSeq 2500 platform using a 50 bp single read on a high output standard v4 flow cell with a 15-20% PhiX spike-in ...
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bioRxiv - Genomics 2021Quote: ... smaller DNA fragments (<100 bp) were purified using the Monarch PCR Purification Kit (New England Biolabs) with a modified protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Circularized ssDNA was next purified and concentrated using a Monarch® PCR & DNA Cleanup Kit (NEB). 20 pmol of the purified product was then incubated with 25 pmol of a complementary primer in 1X T4 DNA ligase buffer (NEB ...
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bioRxiv - Biochemistry 2020Quote: RNA was transcribed from PCR-amplified inserts using the Hiscribe T7 high yield synthesis kit (NEB). 0.1µg/µl of the RNA of interest was heated to 90°Cfor 2 mins and cooled at 4°Cfor 2 mins before adding chilled 10X RNA structure buffer (500mM Tris pH 7.4 ...
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bioRxiv - Microbiology 2019Quote: ... and the linearized DNA was purified using a PCR clean-up kit (New England Biolabs®), eluted into 10 mM Tris (pH 8.0) ...
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bioRxiv - Microbiology 2022Quote: ... The amplicons were purified using the Monarch PCR clean-up kit (New England Biolabs, MA, USA). The purified fragments and the pABC3-His or pABC3-GFP vector were digested with PacI and NotI restriction enzymes (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were amplified and recombined using the NEBuilder HiFi DNA assembly kit (New England Biolabs) followed by Sanger sequencing for identification of positive clones ...
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bioRxiv - Genomics 2020Quote: ... Sheared DNA was purified using either the Monarch PCR and DNA Cleanup kit (NEB, Ipswich, MA) or QIAquick Nucleotide Removal Kit (QIAGEN) ...
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bioRxiv - Microbiology 2022Quote: ... These PCR fragments contained flanking homology for assembly using a Gibson Assembly kit (New England Biolabs) to yield the final vector pMAD2-5’HA-gfpmut2-3’HA (pNW2410 ...
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bioRxiv - Developmental Biology 2022Quote: ... Up to 1000 ng of purified PCR product was used with the HighScribe T7 kit (NEB) and incubated overnight at 37°C ...
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bioRxiv - Bioengineering 2020Quote: ... The PCR product was purified using Monarch® Nucleic Acid Purification Kit (New England Biolabs Inc.).
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bioRxiv - Developmental Biology 2020Quote: ... The amplified products were cloned using the NEB PCR cloning kit (New England Biolabs, Ipswich, MA). Clones from each founder were Sanger sequenced using the NEB analysis primers to define indels.
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bioRxiv - Genomics 2020Quote: ... followed by DNA clean-up using the Monarch PCR & DNA Cleanup Kit (New England BioLabs T1030S). Library preparations were quantified using the using KAPA library quantification kit (Kapa Biosystems KK4824 ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were purified using the Monarch PCR & DNA Cleanup Kit (New England BioLabs., Ipswich, MA, USA). Linearized plasmids ...
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bioRxiv - Microbiology 2021Quote: ... DNA isolation from reaction mixtures was performed using Monarch PCR & DNA Clean-up Kit (NEB, USA). Plasmid miniprep DNA isolation was performed using Wizard Plus SV Minipreps DNA Purification System (Promega ...
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bioRxiv - Neuroscience 2022Quote: DNA was purified using the Monarch PCR and DNA cleanup kit (New England Biolabs, 13-0041) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The assembled product was purified with the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030L) and eluted in 12 μl of H2O.
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bioRxiv - Bioengineering 2022Quote: ... the reaction was cleaned and concentrated with a Monarch PCR & DNA Cleanup Kit (NEB catalog # T1030L) and eluted in either 10 or 12 µL of nuclease free H2O ...
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bioRxiv - Genomics 2022Quote: ... The product was then purified with the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030L), and eluted with 20 μl of ddH2O ...
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bioRxiv - Molecular Biology 2022Quote: ... The synthesized cDNA was purified using Monarch® PCR and DNA Cleanup Kit (New England Biolabs). Poly-guanine was added at the cDNAs 3’ end using Terminal Transferase (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... and SDS samples were concentrated using the Monarch PCR & DNA Cleanup Kit (NEB, South Hamilton, MA). Samples were resolved on a 1% agarose TAE gel with ethidium bromide and imaged using BioRad ChemiDoc MP Imaging system (Hercules ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA purification was performed with the Monarch PCR & DNA Cleanup kit (New England Biolabs, Ipswich, MA), or the DNA Clean & Concentrator Kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2023Quote: ... va mtSSB ΔMTS by PCR amplification and ligation (Q5 Site-Directed Mutagenesis Kit, NEB, Cat #E0554). The plasmid insert was sequence-verified and then transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... The template was gel-purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs, Massachusettes, USA) and transformed into UKMCC1015 electrocompetent cells ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Successful amplicons were purified using the Exo-CIP Rapid PCR Cleanup Kit (New England BioLabs®) and sent for bi-directional Sanger sequencing with Eurofins Genomics.
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bioRxiv - Cancer Biology 2024Quote: ... DNA was amplified by PCR using the Q5 High-Fidelity DNA polymerase kit (New England BioLabs) following the manufacturer’s recommendations and with primers designed around the mutations (supplementary Table S9) ...
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bioRxiv - Biophysics 2023Quote: ... Topoisomerase I treated DNA was purified using Monarch PCR & DNA Cleanup Kit (5 μg) (NEB, T1030S) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The deaminated ΦX174 DNA was purified using Monarch PCR and DNA Cleanup kit (NEB, Ipswich, MA). DNA concentration was quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by genotyping PCR using Phusion PCR protocol (NEB) with primer pair of forward ...
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bioRxiv - Genomics 2021Quote: ... Post-PCR each PCR aliquot was exonuclease I (NEB) treated ...
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bioRxiv - Biochemistry 2023Quote: ... and amplified by PCR using Pfusion PCR polymerase (NEB) with specific primers or random hexamer primers ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR reactions were purified with Monarch DNA Cleanup Kit columns (T1030; New England Biolabs; Ipswich, MA, USA) per the manufacturer’s protocol and eluted in 20 µL nuclease-free water.
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bioRxiv - Molecular Biology 2020Quote: ... The eluted DNA fragments were purified on a Monarch PCR & DNA Cleanup Kit (#T1030S, New England BioLabs and eluted with 26 μL DNAase and RNAase free water ...
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bioRxiv - Microbiology 2021Quote: ... Products of PCR amplification were subjected to KLD reaction using Q5 Site-Directed Mutagenesis Kit (NEB, USA) and transformed into NEB 5-alpha Competent E ...