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Citations for New England Biolabs :
901 - 950 of 4880 citations for Methyl 2 trifluoromethylsulfonyloxy 1 naphthoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... and 25 µL of 2× Hot Start High Fidelity Q5 Master Mix (NEB) were performed ...
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bioRxiv - Genomics 2022Quote: ... The supernatant was loaded on four 2 mL chitin columns (NEB, no. S6651S). Columns were washed with 10 mL of HEGX ...
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bioRxiv - Microbiology 2022Quote: ... We used 2× Luna® Universal qPCR Master Mix (New England Biolabs [NEB]) for quantitative PCR in a volume of 20 µL and primer concentrations of 250 nM each for the forward and reverse primers ...
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bioRxiv - Microbiology 2022Quote: ... We used 2× Luna® Universal qPCR Master Mix (New England Biolabs [NEB]) for quantitative PCR in a volume of 20 µL and primer concentrations of 250 nM each for the forward and reverse primers ...
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bioRxiv - Biochemistry 2022Quote: ... followed by addition of 50 μL of extension mix (1X buffer 2 (NEB), 0.4 mM dNTPs ...
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bioRxiv - Genetics 2022Quote: ... PCRs were performed using Q5 Hot-start 2× master mix (New England Biolabs). Restriction digests were conducted for 2 hours at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... and (MBP)2-HSPC300 proteins were purified through amylose beads (New England Biolabs). The Sra1/Nap1 dimer was obtained by co-expressing His6-Sra1 and untagged Nap1 in Tni insect cells (Expression Systems) ...
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bioRxiv - Microbiology 2023Quote: ... 6 ng of sample was treated with 2 units T4 Polynucleotide Kinase (NEB) and incubated at 37°C for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... the single stranded gRNA oligos were annealed in NEBuffer 2 (New England Biolabs) at 95 °C for 5 min and left to cool ...
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bioRxiv - Genomics 2024Quote: ... at 65 ⁰C for 2 h followed by MspI treatment (NEB Ipswich, MA) at 37⁰C overnight ...
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bioRxiv - Genomics 2024Quote: ... 2 µg of the sgRNA-barcode library was linearized with AscI (NEB, R0558S) and ...
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Emerging cooperativity between Oct4 and Sox2 governs the pluripotency network in mouse early embryosbioRxiv - Developmental Biology 2024Quote: ... 10.0 μl NEBNext High-Fidelity 2×PCR Master Mix (New England Biolabs, M0541S), 0.25 μl 100 μM PCR Index 1 ...
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bioRxiv - Genomics 2024Quote: ... and PCR amplified for 2 cycles using the NEBNext master mix (NEB M0544L) with Illumina Nextera primers and conditions ...
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bioRxiv - Genomics 2024Quote: ... and 21.3 U/µl T4 RNA Ligase 2 Truncated KQ (New England Biolabs) was added to each well and plates were incubated at 4 °C for 18 hours.
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bioRxiv - Microbiology 2024Quote: ... 5 mM MgCl2 and 2 U of recombinant Escherichia coli RNase HI (NEB) were added ...
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bioRxiv - Neuroscience 2024Quote: ... mixed with an equal volume of 2× RNA loading dye (New England Biolabs), was incubated at 75 °C for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... using the NEBNext High-Fidelity 2× PCR Master Mix (New England Biolabs #M0451S) with Nextera DNA CD Indexes (Illumina #20015882) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 mg lysates were incubated with magnetic oligo-DT beads (NEB, S1419S) for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 and 5 minutes by the addition of 0.8 units Proteinase K (NEB). Fragments were resolved on 1% agarose gel and band intensities were quantified on Image Lab (BioRad).
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bioRxiv - Synthetic Biology 2024Quote: ... 2 µL of 10mM dNTPs (complete mixtures of all dNTPs, New England Biolabs, N0447S ...
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bioRxiv - Genomics 2024Quote: ... also containing a final concentration of 2 mM of each dNTP (N0447S, NEB) and 1X Maxima buffer (EP0752 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... GDP hydrolysis was catalyzed via the addition of 2 units of apyrase (NEB) and a further incubation for 2 h ...
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bioRxiv - Biochemistry 2024Quote: ... and Q5 Hot Start High-Fidelity 2× Master Mix (NEB, catalog no. M0494S) in a 50 µL reaction ...
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bioRxiv - Biochemistry 2024Quote: ... The cleared extract was loaded onto 2 ml pre-equilibrated amylose resin (NEB). The column was washed extensively with amylose buffer (25 mM HEPES-NaOH pH 6.8 ...
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bioRxiv - Bioengineering 2024Quote: ... IVT templates were degraded with 2 µL of DNase I (New England Biolabs) per IVT reaction for 20 min at 37 °C ...
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bioRxiv - Genomics 2022Quote: ... 1 μl of Exonuclease 1 (NEB, M0293S) was added to each PCR product and incubated at 37C for 15 min to digest any single-stranded product ...
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bioRxiv - Molecular Biology 2024Quote: ... in 1× NEB buffer 1 (NEB, B7001S) for 15 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 1% cholorophorm was added together with 10 μg mL-1 DNase 1 (NEB) and 1 μg mL-1 RNase A ...
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bioRxiv - Molecular Biology 2024Quote: ... The four PCR products were then assembled in a 1:1:1:1 ratio using HiFi assembly (NEB, E5520S) according to the manufacturer’s instructions to create the pLenti-STARR vector (pAS5018) ...
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bioRxiv - Genetics 2021Quote: ... 2 µL of Taq DNA polymerase (LongAmp Taq DNA Polymerase kit, New England Biolabs), 7.5 µL of dNTPs (dNTP set ...
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bioRxiv - Developmental Biology 2021Quote: ... and 2 hours at 55°C with 0.1 mg/ml Proteinase K (NEB P8107S).
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bioRxiv - Molecular Biology 2022Quote: pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-Puro was opened with NdeI (NEB) and AfeI (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... and then the extracted RNA was treated with 2 units of DNase I (NEB) and further cleaned up via phenol-chloroform (pH 4.3 ...
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bioRxiv - Genomics 2020Quote: ... 2 μl 10 mM dATP and 5 μl Klenow Fragment (3’ −> 5’ exo-) (NEB) for A-tailing at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and 15 μL of 2× Phusion High-Fidelity PCR Master Mix (New England Biolabs). All PCR amplification reactions were carried out on a T100 Thermal Cycle (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... 5’ end repair was done using T4 PNK with 2 mM ATP (NEB P0756S). Following RNA purification with Zymo Oligo Clean & Concentrator (D4060) ...
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bioRxiv - Molecular Biology 2020Quote: ... Barcoded pre-adenylated linkers were ligated using T4 RNA ligase 2 truncated K227Q (NEB) and rRNA was depleted using the Ribo-Zero gold kit (Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were quenched with 2% H2O2 and digested in 10µg/ml Proteinase K (BioLabs). The reaction was stopped with 2mg/ml Glycine (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... The nuclei were washed two times with a plastid-lysis buffer (NEB + 2% triton) followed with centrifugation (5 min at 1500 g ...
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bioRxiv - Bioengineering 2020Quote: ... half of the DNA was mixed with 2 μL ExoI (M0293S, New England Biolabs), 1 μL of Lambda Exonuclease (M0262S ...
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bioRxiv - Bioengineering 2020Quote: ... 2 μL of PCR mixture was used for 10 μL KLD reaction (NEB, MA), which proceeded under room temperature for 1 hour ...
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bioRxiv - Plant Biology 2021Quote: ... for level 2 reaction together with T4 DNA Ligase (New England BioLabs, Ipswich, UK). TCSn promoter DNA sequence (Zürcher et al. ...
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bioRxiv - Biochemistry 2021Quote: ... and 2 µg DNA was treated with uracil DNA glycosylase (New England Biolabs, Inc.) at 37 °C for 60 min ...
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bioRxiv - Systems Biology 2021Quote: ... 2 micrograms of each library was digested with SphI-HF and SpeI-HF (NEB) and then treated with Antarctic phosphatase (NEB).
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bioRxiv - Systems Biology 2020Quote: ... Samples were digested using 2 Units/μL MNase in 20μL MNase Digestion Buffer (NEB) for 5 minutes at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... for 10 min with 2 mM Ribonucleoside-Vanadyl Complex RVC (New England Biolabs, S1402S) at room temperature and then permeabilized for 5 min on ice in 0.5% Triton-X with 2mM RVC ...
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bioRxiv - Plant Biology 2021Quote: ... for 3 hours at 55°C and M-2 was digested with XhoI (NEB) overnight at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... and cloning was performed using Gibson Assembly 2× Master Mix (New England BioLabs, NEB) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and cloning was performed using Gibson Assembly 2× Master Mix (New England BioLabs, NEB) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... About 2-5μg of fragmented DNA was digested with 40U RNaseH (New England Biolabs) for at least 24 hours at 370C to serve as a negative control ...