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Citations for New England Biolabs :
901 - 950 of 1653 citations for IL 10 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... resuspended in 500 μL 10 mM Tris-HCl buffer and digested with DpnII (NEB, R0543) overnight at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The cosmid-I95 plasmid was amplified in a NEB 10-beta (New England Biolabs, C3019H), and the DNA was purified using a QIAfilter Plasmid Midi Kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluent was subsequently mixed with 10 U of Bst 2.0 WarmStart DNA polymerase (NEB) and 1× Q5 polymerase reaction buffer (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μg of plasmids were then digested overnight with the restriction enzyme CspCI (NEB, #R0645S) in a total volume of 50 μL ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The 10-µl reactions contained: 5 µl Luna Universal qPCR Master Mix (New England Biolabs), 0.25 µl forward primer (10 µM) ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was further purified on a 5-10 mL amylose column (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted using the rRNA depletion Kit (Human/Mouse/Rat) (New England BioLabs [NEB, USA]). Library preparation for total RNA sequencing was performed using NEBNext® UltraTM II RNA Library Preparation Kit for Illumina (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted using the rRNA depletion Kit (Human/Mouse/Rat) (New England BioLabs [NEB, USA]). Library preparation for total RNA sequencing was performed using NEBNext® UltraTM II RNA Library Preparation Kit for Illumina (NEB ...
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bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibody (1:10.000, used with 1:10.000 secondary horseradish peroxidase-conjugated anti-mouse antibody; NEB).
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bioRxiv - Neuroscience 2022Quote: ... CREB (CCDS15005.1) and CRTC1 (CCDS40372.1) were amplified from mouse brain cDNA library by primers contain AgeI-HF (R3552L, NEB) and BamHI-HF (R3136L ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was depleted of ribosomal RNAs using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs) before they were used for library construction using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) ...
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Evaluation of the OsTIR1 and AtAFB2 AID systems for chromatin protein degradation in mammalian cellsbioRxiv - Molecular Biology 2021Quote: ... mCapH2 (Gene ID: 52683) genes were PCR amplified from human and mouse genomic DNA with Q5 polymerase (NEB). The lengths of homology arms are featured in electronic supplementary material Table S2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Removal of rRNA was carried out by use of the NEBNext rRNA Depletion Kit Human/Mouse/Rat (NEB) followed by strand-specific cDNA NGS library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina ...
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bioRxiv - Neuroscience 2024Quote: Mouse Hapln1 (NM_013500) was cloned into the pAAV vector by PCR with the following primers and ligase (NEB) or In-Fusion cloning (Takara) ...
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PD-1 checkpoint blockade disrupts CD4 T cell regulated adaptive B cell tolerance to foreign antigensbioRxiv - Immunology 2021Quote: ... 10 ng of genomic DNA was amplified using LongAmp Taq 2X Master Mix (New England BioLabs) and the provided barcoded 16S primers ...
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bioRxiv - Cell Biology 2019Quote: ... We incubated cells in complete medium containing 10 μM of SNAP-Cell Block (New England Biolabs) to block all available pre-existing SNAP-tag (Quench) ...
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bioRxiv - Genetics 2021Quote: ... we digested 10 μg of each vector with AvrII (#ER1561; Thermo Fischer) and XcmI (#R0533; NEB) and performed a gel-purification ...
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bioRxiv - Genomics 2020Quote: ... 1 μL of 10 mM dATP and 15 units of Klenow 3’ → 5’ exo- (NEB M0212L) was added to blunted ends and incubated at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... The 5’-end of dephosphorylated mRNAs were then labeled using 10 units of T4 PNK (NEB) and 0.5 µl [γ-32P] ATP (6000 Ci/mmol ...
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bioRxiv - Molecular Biology 2020Quote: 10 μg of genomic DNA was digested overnight with 20 units of AluI and MboI (NEB) and then precipitated with isopropanol ...
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bioRxiv - Genomics 2022Quote: ... 10 μl 2X Quick T4 ligase buffer and 2 μl Quick T4 DNA ligase (NEB, M2200L) were added to the reaction and incubate at 37 °C for overnight ...
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bioRxiv - Genomics 2020Quote: ... PCR amplification (8-9 cycles) was performed with 10 μL Fusion HF Buffer (New England Biolabs), 3.125 μL 10uM TruSeq Primer 1 ...
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bioRxiv - Genomics 2020Quote: The polyadenylation product was mixed with 0.5 μL 10 mM dNTPs (New England Biolabs, Cat. # N0447L), 1 μL reverse transcription primers (25 ng/μL ...
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bioRxiv - Genetics 2019Quote: ... The reannealed amplicon was then digested with 10 units of T7 Endonuclease I (New England Biolabs) at 37 °C for 90min ...
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bioRxiv - Neuroscience 2019Quote: ... and then mounted using 10 μl ProLong Gold Antifade Reagent containing DAPI (9071S, New England Biolabs). The slides were imaged when the mounting medium was fully cured >12 h.
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bioRxiv - Molecular Biology 2019Quote: ... 10 mM CaCl2 and 1x PIC) containing 2U/μl of micrococcal nuclease (New England BioLabs, M0247S) and incubating on ice for 20 min and then 37°C for 10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μl reaction mixture composed by 10 μl of Phusion High-Fidelity Master Mix 2X (NEB), 2 μl of Betaine 5M (Sigma-Aldrich) ...
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bioRxiv - Physiology 2021Quote: ... RNA 3’ end dephosphorylation reaction consisted of T4 PNK (20 U/10 μL sample, NEB M0201S), SuperaseIn in 1X T4 PNK buffer without ATP for 60 min at 37 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 µl of PCR product was then mixed with 1.5 µl 10X NEBuffer 2 (B7002S, NEB) and 1.5 µl of Nuclease-free water ...
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bioRxiv - Genomics 2021Quote: ... DNA samples were labeled with 10 μL of a cocktail of repair enzymes (PreCR mix, NEB), fragmented ...
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bioRxiv - Genomics 2020Quote: ... using 10 units each of MluCI and NlaIII restriction enzymes (New England Biolabs, Beverly MA, USA), NEB CutSmart buffer ...
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bioRxiv - Bioengineering 2020Quote: ... The post-transcription reaction mixture was incubated with 10 units of DNase I (New England Biolabs) at 37°C for 1 hour to remove the gblock template then purified using a RNA spin column (Zymo Research) ...
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bioRxiv - Biochemistry 2021Quote: ... RCA products were debranched using 10 units/ µg DNA of T7 Endonuclease I (New England Biolabs) for 15 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of crude mitochondria were mixed with 1/10 volume gel loading dye (7025, NEB) and subjected to Semi-Denaturing Detergent Agarose Gel Electrophoresis (SDD-AGE) ...
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bioRxiv - Microbiology 2020Quote: ... We incubated 10 pmol gRNA with 5 pmol SauCas9 (New England Biolabs [NEB], Ipswich, MA, USA) at 25 °C for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... We incubated 10 pmol gRNA with 5 pmol SauCas9 (New England Biolabs [NEB], Ipswich, MA, USA) at 25 °C for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... 1 μL of 10 mM ATP and 1 μL of T4 DNA ligase (New England Biolabs) were added and the ligation reaction was incubated for 5 cycles at 20°C for one hour followed by 37°C for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then treated 10 min with 1 U DNase I (New England BioLabs, Ipswich, MA) at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... 10 ml of T7 enzyme mix and 12.5 ml of 5’GpppA cap analog (S1406S, NEB) in a final reaction volume of 100 ml for 30 min at 30°C ...
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bioRxiv - Cell Biology 2022Quote: ... PV stock solution (1 mM) was prepared by adding 10 μl of 100 mM vanadate (NEB) and 50 μl of 100 mM hydrogen peroxide (diluted from a 30 % stock in 20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... 2 µl T4 DNA ligase buffer and 2 µl 10 mg/ml BSA (New England Biolabs). These reactions were placed in a thermocycler with following program ...
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bioRxiv - Microbiology 2022Quote: ... A 20 μL ligation reaction was performed with 10 μL Blunt/TA ligase master mix (NEB), 100 ng of vector backbone ...
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bioRxiv - Biochemistry 2019Quote: ... reaction mixtures (10 μL) applied the Phusion DNA polymerase (0.2 U final concentration, New England Biolabs) and buffer (5× diluted final concentration ...
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bioRxiv - Genetics 2019Quote: ... Approximately 250 ng of the purified products were digested with 10 U T7EI (New England Biolabs) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2019Quote: ... 10 μl of NEBNext Quick Ligation Buffer and 1.5 μl of T4 DNA Ligase enzyme (NEB). The mixture was incubated for 10 minutes at 65°C followed by incubation on ice for 2 minutes and then directly used as a template for the next step of the library prep which is cDNA synthesis according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... 2-10 ng of starting input genomic DNA was digested with 30 units of MspI (NEB). Fragments were ligated to pre-annealed adapters containing 5’-methyl-cytosine instead of cytosine according to Illumina’s specified guidelines ...
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bioRxiv - Synthetic Biology 2020Quote: ... isothermal DNA assembly57 was conducted at the scale of 10 μL by incubating T5 exonuclease (NEB), Phusion polymerase (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... End repair reactions were conducted by adding 10 μL of 10x T4 DNA ligase buffer (NEB), 4 μL of 10 mM dNTPs ...
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bioRxiv - Microbiology 2021Quote: ... 10 μL Luna Universal qPCR Master Mix (New England Biolabs Inc., Bionordika Denmark A/S, Denmark), 1.4 μL of each primer (10 μM) ...
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bioRxiv - Biophysics 2020Quote: ... and were 5’ end-radiolabeled with [Ɣ-32P]-ATP using 10 U T4 polynucleotide kinase (NEB). Radiolabeled transcripts were purified on polyacrylamide gel (6% acrylamide:bisacrylamide 19:1 ...