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Citations for New England Biolabs :
901 - 950 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... 2 μl of the assembly reaction was transformed into 5 μl chemically competent cells (E. coli NEB 5-alpha, New England Biolabs). Circuit constructs were analyzed by PCR ...
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bioRxiv - Genetics 2023Quote: ... 10 µg of the K3L variant library was digested with 5 µL of BstEII-HF and 5 µL SacI-HF (NEB Cat#R3156S) in a 50 µL reaction to generate a linear insert fragment of approximately 1130bp ...
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bioRxiv - Microbiology 2024Quote: ... The variable region V3+V4 of the 16S rRNA gene was amplified using a broad-range primer pair (338F: 5’-ACTCCTACGGGAGGCAGCA-3′, 806R:5′-GGACTACHVGGGTWTCTAAT-3′) using the Phusionâ High-Fidelity PCR Master Mix (New England Biolabs, Beverley, MA). The PCR amplification program was as follows ...
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bioRxiv - Cell Biology 2020Quote: 3′ linker ligation (1x PNK buffer, 20 U T4 RNA ligase I (NEB), 20 U T4 RNA Ligase II truncated K227R ...
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bioRxiv - Genetics 2021Quote: ... Injection mix for Cas9 enzyme included Cas9 enzyme (20 μM, New England BioLabs), 2.5 μl of 4x Injection Buffer (0.2% phenol red ...
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bioRxiv - Developmental Biology 2021Quote: ... Restriction digest at 37°C for 20 minutes with Msp1 (New England Biolabs) was performed to cleave the wildtype ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μL of 1× PNK mix (2 μL of 10× PNK buffer (NEB), 2 μL ATP (SCP801 ...
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bioRxiv - Molecular Biology 2021Quote: ... 2X hybridisation buffer (4X SSC, 20% dextran sulphate, 2 mg/mL BSA (NEB), 1/10 volume nuclease free water and 1/10 volume vanadyl-ribonucleoside complex (VRC ...
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bioRxiv - Genetics 2020Quote: ... Injection mixes contained 1.30 μl of Cas9 enzyme (20 μM, New England BioLabs), 1.60 μl of prepared gRNAs ...
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bioRxiv - Genomics 2021Quote: ... Reactions were diluted to 100 µl and 20 U of exonuclease I (NEB) added and incubated at 37 °C before purification using a 0.8:1 ratio of AMPure XP beads ...
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bioRxiv - Molecular Biology 2019Quote: ... 1x EvaGreen (Biotum) and 20 U/ml Q5 DNA polymerase (New England Biolabs). The reaction components were prepared as a 2x-master mix and 5 µl of the mix was pipetted into opaque wells of 384-well PCR-plate (Bio-Rad) ...
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bioRxiv - Systems Biology 2021Quote: ... and cloned into the libraries using multiple 20 microliter ligations (NEB T4 ligase). The libraries were transformed into 5-alpha electrocompetent cells (NEB ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl BSA (20 mg/ml) (New England Biolabs Inc., Ipswich, MA, USA), 10 μl Brilliant III Ultra-Fast SYBR® Green Low ROX qPCR Master Mix (Agilent Technologies ...
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bioRxiv - Microbiology 2019Quote: ... was linearized with 20 units/µg EcoRI-HF® (New England Biolabs®) and the linearized DNA was purified using a PCR clean-up kit (New England Biolabs®) ...
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bioRxiv - Genetics 2022Quote: ... followed by digestion with 55 µL of proteinase K (NEB, 20 mg/mL) for 30 min at 50 °C ...
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bioRxiv - Immunology 2022Quote: ... TCRs were then amplified by PCR (20 cycles with the Phusion from NEB) with a single primer pair binding to the constant region and the adapter linked to the TRAV/TRBV primers added during the reverse transcription ...
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bioRxiv - Biochemistry 2021Quote: ... 500 µL supernatant was applied to 20 µL amylose resin (New England Biolabs) and incubated for two hours at 4°C ...
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bioRxiv - Genetics 2022Quote: ... Each reaction contained 20 μL and used Q5 polymerase (New England Biolabs, #M0491). The reagents were distributed into each well of a 96 well plate as follows ...
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bioRxiv - Microbiology 2019Quote: ... cholerae RNA at 37°C with 20 units of T4 RNA ligase (NEB) in a 20 µL reaction containing 1X T4 RNA ligase buffer ...
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bioRxiv - Microbiology 2019Quote: ... 20] and LongAmp™ Taq 2X Master Mix (New England Biolabs, Ipswich, MA). Amplification was performed using an Applied Biosystems Veriti™ Thermal Cycler (Thermo Fischer Scientific ...
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bioRxiv - Genomics 2019Quote: ... 2 µg of genomic DNA was digested with 20 units of MspI (NEB) overnight at 37C in a volume of 100 µl ...
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bioRxiv - Microbiology 2019Quote: ... 20 µl of 10x DNase buffer and 4 µl DNase I (NEB Inc.) were added and incubated at 37°C for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... Sticky ends were created by injecting 20 units of USER enzyme (NEB, M5505S) and incubating with 50 μL of 1X TA buffer (Teknova ...
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bioRxiv - Genomics 2021Quote: ... samples were incubated with 20 μl Oligo d(T) Magnetic Beads (NEB, S1419S) in PCR strips for selection of polyadenylated (poly(A) ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were digested with 20 units of XmnI and XcmI respectively (NEB) to distinguish cleavable mutant from un-cleavable wild-type amplicons ...
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bioRxiv - Genetics 2020Quote: ... and a degenerate 20-mer (primer jklab0172) was inserted by HiFi Assembly (NEB). The resulting barcoded clone pools were transformed ...
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bioRxiv - Genomics 2020Quote: ... 10 µ of 20 mg/mL BSA (New England Biolabs, catalog no. B9000S), and 930 µ of nuclease-free water ...
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bioRxiv - Biophysics 2021Quote: ... by adding 1 µl (20 units) of BamHI restriction enzyme (New England Biolabs) to 1 µg DNA ...
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bioRxiv - Genomics 2020Quote: ... followed by addition of 20 μl Ligation master mix (containing: 15 μl NEB Quick ligation buffer ...
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bioRxiv - Biochemistry 2021Quote: ... 300 nM MS2-MBP was prebound to 20 μL of amylose resin (NEB) in EMB 300 Buffer ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was passed through a 20 ml amylose resin (New England BioLabs) and washed with 10 column volumes (CVs ...
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bioRxiv - Genomics 2020Quote: ... 15 mM HEPES pH 8) and 20 μl of DNase I (M0303S, NEB). The Nuclease Flushing mix was loaded into the flow cell and incubated for 30 min ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA (20 ng) was PCR amplified using LongAmpTaq 2X master mix (NEB). The PCR amplicons of selected regions were subjected to Sanger sequencing and BLAST analysis to confirm the presence of eight variants using the primers listed in Supplemental Table 1.
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bioRxiv - Immunology 2022Quote: ... ∼50 μg of protein were incubated with 20 units of λPP (NEB, USA) for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... after which the template was removed by treatment 20 U of DpnI (NEB), a methylation-sensitive restriction enzyme ...
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bioRxiv - Synthetic Biology 2022Quote: ... for assembly into levels 2 in 20 μl 1x T4 ligase buffer (NEB). The reaction condition was ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.0125 μl Exonuclease I (20 U/ml; New England Biolabs, cat. no. M0293S) and 2.36 μl of water were added ...
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bioRxiv - Molecular Biology 2024Quote: ... with the addition of 20 U of Murine Rnase Inhibitor (New England Biolabs). qPCR reactions were run in 12 μl with PowerUpTM SYBRTM Green Master Mix (Applied Biosystems ...
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bioRxiv - Genomics 2024Quote: ... After adding 10 µL of 20 mg/µL proteinase K (New England Biolabs), proteins were degraded for 30 minutes at 55°C followed by an overnight incubation at 68°C with shaking to reverse crosslinks ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 µL supernatant was applied to 20 µL amylose resin (New England Biolabs) and incubated for two hours at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... 0.05% Tween-20) containing 10 µMnt of circular ssDNA (PhiX174 virion DNA, NEB) immobilised on magnetic Streptavidin-coated beads (Dynabeads M-280 ...
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bioRxiv - Bioengineering 2023Quote: ... and 20 units/mL of Vent® (exo-) DNA Polymerase (NEB, cat# M0257L) or alternatively with Taq (NEB ...
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bioRxiv - Immunology 2023Quote: ... 500ng of gDNA was digested with 20 units XbaI (New England Biolabs, Germany) for one h ...
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bioRxiv - Genomics 2023Quote: ... 2X hybridisation buffer (4X SSC, 20% dextran sulphate, 2 mg/mL BSA (NEB), 1/10 volume nuclease free water and 1/10 volume vanadyl-ribonucleoside complex (VRC ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by addition of 20 µl of 0.5 mg/ml RNase A (NEB) and incubation at 37°C for 1 hour ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and treated with 1 µL (20 units) of DpnI (New England Biolabs, R0176L) for 1 h at 37°C before being purified on magnetic beads ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μg genomic DNA was digested with 20 units Sty I (NEB, R3500S) at 37 °C for 3 h ...
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bioRxiv - Genomics 2021Quote: ... 5’-deadenylase (Cat. No. M0331S; NEB; use 0.5 uL), PEG 8000 (final concentration = 10% (w/v)) ...
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bioRxiv - Cell Biology 2020Quote: ... an oligonucleotide was 5’-labeled using PNK enzyme (NEB). The ITS2 probe was previously reported (Donati et al. ...
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bioRxiv - Genomics 2021Quote: ... 5 U of Klenow Fragment (New England Biolabs, M0210) were added and incubated at 25°C for 20 minutes ...