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Citations for New England Biolabs :
851 - 900 of 1339 citations for Synapsin I derived peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Lysates were then successively treated with 8 U/mL DNase I (NEB; M0303S) for 5 min at 37°C and 4 U/mL RNase I for 3 min at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... sections were treated with DNase I (10 U/mL, New England Biolabs, M0303S) for 1 h at 37 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by a DNase I treatment (New England Biolabs GmbH, Frankfurt, Germany, #M0303). Subsequent cDNA synthesis was conducted with qScriber cDNA Synthesis Kit (highQu ...
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bioRxiv - Immunology 2023Quote: ... 10 U of Exonuclease I and 20U of Exonuclease III (New England Biolabs) was added to each reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM CaCl2) combined with DNase I (10 U = 5 μl; NEB, M0303L) at 37°C for 40 minutes ...
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bioRxiv - Biophysics 2023Quote: Following transcription we digest the DNA template with DNase I (New England Biolabs), and purify the RNA transcripts using an RNeasy mini kit (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ... Pre-amplified products were treated with Exonuclease I (New England Biolabs, Ipswich, USA) for 30 min at 37°C to eliminate the carryover of unincorporated primers ...
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bioRxiv - Molecular Biology 2022Quote: ... The total RNA was further treated with RNase-free DNase I (NEB, M0303) and reverse-transcribed using the random hexamer and Superscript II (Invitrogen/Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... mRNA was radiolabelled at the 3’ end using T4 RNA ligase I (NEB) and [32P] pCp (Perkin Elmer ...
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bioRxiv - Cell Biology 2023Quote: ... 1ug of DNA was treated with 10U of Topoisomerase I (New England Biolabs) at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2023Quote: ... according to the manufacturer’s protocol and treated with DNAse I (New England Biolabs). Quantitative real time PCR was run on Applied Biosystems QuantStudio 7 Flex (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Both pDN0605 and pKB11 were digested with I-SceI (New England Biolabs, R0694S) and I-CeuI (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μg genomic DNA was digested with 20 units Sty I (NEB, R3500S) at 37 °C for 3 h ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation for 1 hr with 4 units of DNase I (NEB) at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... the intermediate library was digested with I-SceI (New England Biolabs, Ipswitch, MA) and NheI-HF (New England Biolabs ...
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bioRxiv - Genomics 2024Quote: ... and 2 U/µl Thermolabile Exonuclease I (New England Biolabs, catalog no. M0568S) and incubated for 15 min at 37 °C on a shaking thermoblock at 400 rpm ...
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bioRxiv - Immunology 2024Quote: ... isolated RNA samples were treated with RNase-free DNase I (New England Biolabs) to remove any contaminant DNAs which might yield false positives in downstream PCR tests ...
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bioRxiv - Immunology 2024Quote: One µg of SQBs was incubated with 1.0 U/µL DNase I (NEB) with 10 × DNase I buffer diluted in water (New England Biolabs #M0303S) ...
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bioRxiv - Immunology 2024Quote: ... genomic DNA was digested on-beads using 1U of DNAse I (NEB M0303) at room temperature for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... the library was digested with the restriction enzyme Dpn I (New England BioLabs), which possesses specificity for methylated 5’-GmATC-3’ sequences ...
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bioRxiv - Immunology 2023Quote: ... and excess primers were removed with an Exonuclease I digestion (New England Biolabs). Whole transcriptome amplification (WTA ...
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bioRxiv - Molecular Biology 2020Quote: ... pUHES:UmPit2+SP:mCherry with its natural signal peptide was generated via NEBuilder HiFi DNA Assembly Cloning Kit (NEB) by combining PCR-amplified UmPit2+SP:mCherry using primers 2127 + 2128 and PCR-amplified pUHESdest using primers 2125 + 2126 without UhAvr1 signal peptide ...
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bioRxiv - Cell Biology 2020Quote: ... These peptides were used as the substrate in a PKA kinase assay kit (New England Biolabs, #P6000S) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... These peptides were used as the substrate in a PKA kinase assay kit (New England Biolabs, #P6000S) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... then deglycosylated with 2000 U of Peptide-N-Glycosidase F (PNGase F) (New England BioLabs, MA, USA). All samples were incubated for 16 hours at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... 3mM MgCl2) for 5 min before digesting with 0.2U/ml of DNase I (NEB). Embryos were then fixed in 4% PFA ...
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bioRxiv - Cell Biology 2020Quote: ... 3 µg of purified DNA was digested with 10 units I-SceI (NEB, R0694), 10 units I-CeuI (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was further purified by treatment with DNase I (RNase-free, New England Biolabs) and heat inactivation of the enzyme as recommended by the manufacturer.
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bioRxiv - Molecular Biology 2021Quote: ... and then the extracted RNA was treated with 2 units of DNase I (NEB) and further cleaned up via phenol-chloroform (pH 4.3 ...
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bioRxiv - Genomics 2022Quote: ... and then the reactions were treated with 200 U/mL of Exonuclease I (NEB) for 1 h at 37°C ...
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bioRxiv - Genomics 2019Quote: ... Then the in vitro transcribed mRNAs were treated with DNase I (NEB, Cat. M0303S) to remove the plasmid DNA template ...
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bioRxiv - Genomics 2020Quote: ... After RNA isolation 10μg of RNA per sample was treated with DNase I (NEB) then column purified using a RNeasy MinElute kit (Qiagen) ...
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bioRxiv - Genomics 2021Quote: ... and 8 μL of 5U/μL DNA Polymerase I Large (Klenow) Fragment (NEB M0210). The reaction was the incubated at 37°C in a Thermomixer at 900 rpm for 45 minutes.
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bioRxiv - Genomics 2020Quote: ... The PCR products were treated with exonuclease I and alkaline phosphatase (NEB, M0293; M0371) and Sanger sequenced to verify successful knockouts ...
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bioRxiv - Biochemistry 2021Quote: ... Extra RT primers were removed by Exonuclease I (100 U; M0293S, NEB, Ipswich, MA) for 2 hr at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... the free primer was removed by Exonuclease I (80 U; M0293S, NEB, Ipswich, MA), and a 3’ DNA adaptor was ligated onto the cDNA product with T4 RNA Ligase 1 ...
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bioRxiv - Microbiology 2020Quote: ... 6µg of total RNA was treated with RNase-free DNase I (New England Biolabs) for 3 h at 37 °C to remove contaminating chromosomal DNA and precipitated with 0.1 volume of 3 M sodium acetate (pH 5.2 ...
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bioRxiv - Biophysics 2020Quote: ... and 7U/mL Klenow Fragment of DNA Polymerase I (3’-5 exo-) (NEB, M0212). Reactions were incubated at 37°C and incorporation of labeled dCMP was monitored by an acid precipitation assay ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by 30 minute incubation at 37°C with Exonuclease I (New England BioLabs) to digest single stranded
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bioRxiv - Microbiology 2020Quote: ... Contaminating DNA was degraded by treatment with RNase-free DNase I (New England Biolabs) for 10 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... samples were stored on ice while adding DNase I (New England Biolabs, Ipswich, MA): 4 µl DNase I (2 U/µl) ...
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bioRxiv - Developmental Biology 2020Quote: ... Rehybridized product was digested with 1 μL T7 endonuclease I enzyme (New England Biolabs) at 37°C for 1 hour and separated on a 1.5% agarose gel ...
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bioRxiv - Systems Biology 2019Quote: ... The RNA and free primers were degraded using 20U of Exonuclease I (NEB, M0293L) and 50U RNaseIf (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... the DNA template was digested with 2 units of DNase I (New England Biolabs) for 1 h at 37 °C and the RNA was desalted twice by Illustra Microspin G-25 columns (GE Healthcare Life Sciences) ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCRs were performed using either: (i) the Q5 High-Fidelity 2x Master Mix (NEB), followed by DpnI (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... and then digested by 2 U of T7 endonuclease I (T7E1, New England BioLabs) at 37 °C for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... RNA preparations were treated with DNase I to remove genomic DNA (New England Biolabs) and quantified using NanoDrop (ThermoFisher) ...
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bioRxiv - Plant Biology 2020Quote: ... One microgram of total RNA was then treated with DNase I (New England Biolabs) prior to the first-strand cDNA synthesis using AffinityScript RT-qPCR cDNA synthesis kit (Agilent Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... Fragmented DNA was end-repaired using the NEBNext Ultra I kit (New England Biolabs). The fragments were then ligated with an adapter ...
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bioRxiv - Molecular Biology 2021Quote: ... Template DNA was removed by the addition of DNase I (Cat. No. M0303S, NEB) and in vitro synthesized RNA was subsequently purified using Monarch RNA Cleanup Kit (Cat ...