Labshake search
Citations for New England Biolabs :
851 - 900 of 10000+ citations for Mouse WD repeat containing protein WRAP73 WRAP73 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... primers containing overhangs of the 3x FLAG peptide were phosphorylated using the T4 polynucleotide kinase (New England BioLabs). Phosphorylated primers were used to PCR amplify pQLinkN-pgaD to generate the plasmid pQLinkN-pgaD-FLAG ...
-
bioRxiv - Immunology 2021Quote: ... the samples were added to 2 μL of second-strand synthesis mix containing 2.25× NEB buffer 2 (NEB), 0.625mM each dNTP Mixture (NEB) ...
-
bioRxiv - Systems Biology 2020Quote: ... and second strand synthesis was carried out by resuspending beads in a master mix containing Klenow Fragment (NEB), dNTPs ...
-
bioRxiv - Cancer Biology 2020Quote: ... DNA-bound beads were resuspended in 100µl of mix containing 82µl of 1X NEB T4 DNA ligase buffer with 10mM ATP (NEB), 10µl of 10 (2.5mM each ...
-
bioRxiv - Genetics 2021Quote: ... Different SCON variant fragments containing the respective complementary ends were then inserted into eGFP with SapI (R0569S, NEB) and T4 ligase for 20 cycles of 2 minutes at 37 °C and 5 minutes at 16 °C ...
-
bioRxiv - Biochemistry 2022Quote: AP-DNA was prepared by incubating 1 μM uracil-containing oligonucleotide with 0.6 U UDG (New England Biolabs) (55 ...
-
bioRxiv - Microbiology 2022Quote: ... The cDNA was used as template in PCR reactions containing the Taq 2X Master Mix (New England Biolabs) and 5 μM of MP2- and scorpine-specific primers (Table S7) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Non-overlapping forward and reverse primers containing the desired mutation were phosphorylated by polynucleotide kinase (New England Biolabs) as per the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were collected and incubated in fresh SM containing 10 µM SNAP-Cell Block (New England Biolabs S9106), for 30 min at 25°C ...
-
bioRxiv - Cell Biology 2019Quote: ... 50mM Tris (pH 8.5-8.8)) containing protease and phosphatase inhibitors (cOmplete Mini, PhosSTOP, Roche; 2mM Sodium Orthovanadate, NEB), sonicated to shear chromatin and centrifuged to remove cellular debris ...
-
bioRxiv - Molecular Biology 2020Quote: ... EcoRV de-staining buffer (deionized water containing 1X cut-smart buffer and 200 units of EcoRV (NEB, #R3195S) in 500ul) ...
-
bioRxiv - Molecular Biology 2020Quote: ... DNase I de-staining buffer (PBS containing 1X DNase buffer and 20 units of DNase I (NEB, #M0303) in 500ul) ...
-
bioRxiv - Microbiology 2021Quote: ... Transposon-containing fragments were enriched by PCR using ComP7 primers ComP5 using Phusion DNA polymerase (New England Biolabs) in a 20-cycle reaction ...
-
bioRxiv - Microbiology 2021Quote: ... The PCR for each sample was performed in 50µl total volume: containing 0.5µl Q5 High-Fidelity DNA polymerase (New England Biolabs, USA), 10µl 5X Q5 buffer ...
-
bioRxiv - Biochemistry 2019Quote: ... streptavidin tips were incubated overnight in 50 mM ammonium bicarbonate containing 1,000 units PNGase F (New England Biolabs) at 37 °C ...
-
bioRxiv - Genomics 2021Quote: ... 1uL of the oligo mix was added to a master mix containing 1x T4 ligase buffer (NEB M0202M), 0.5 uL T4 PNK (NEB M0201L ...
-
bioRxiv - Biochemistry 2021Quote: ... fully assembled complexes containing His-Rpn12 and MBP-Rpn6 were purified using a HisTrap and amylose resin (NEB), and cleaved with HRV-protease ...
-
bioRxiv - Biochemistry 2021Quote: ... 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1× NEB 1 buffer ...
-
bioRxiv - Cell Biology 2020Quote: 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1x NEB 1 buffer ...
-
bioRxiv - Genomics 2021Quote: ... in a 25 μL reaction containing 1X PNK buffer and 15 units of polynucleotide kinase (New England Biolabs) by incubating at 37°C for 45 minutes ...
-
bioRxiv - Immunology 2020Quote: ... before amplification with Illumina adapter-containing primers (Nextera i7 indices) and NEBNext Ultra II Q5 Master Mix (NEB) as follows ...
-
bioRxiv - Molecular Biology 2021Quote: ... Beads were resuspended in Fast AP Master Mix (1x Fast AP Buffer containing 80U RNase Inhibitor (M0314, NEB), 2U TURBO DNase (AM2238 ...
-
bioRxiv - Immunology 2021Quote: ... and second strand synthesis was carried out by resuspending beads in a master mix containing Klenow Fragment (NEB), dNTPs ...
-
bioRxiv - Molecular Biology 2023Quote: ... A mixture containing 1 μl of a 4-fold dilution of Low Range ssRNA Ladder (New England Biolabs) and 0.75 pmol each of RPIX_SC1_Bridge ...
-
bioRxiv - Microbiology 2022Quote: ... pRS1 containing the YohP-MS2-6xbs coding sequence was first linearized by EcoRI restriction digest (NEB, Frankfurt, Germany). In vitro transcription was performed using AmpliScribe T7-Flash Transcription kit (Lucigen Corp. ...
-
bioRxiv - Molecular Biology 2023Quote: ... MYH7 fragment containing mutation was amplified by PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) and 500 nM forward and reverse primers (Table 3) ...
-
bioRxiv - Biophysics 2023Quote: ... p-SFG retroviral backbone containing an IRES-ΔCD19 reporter gene was linearized by NotI-HF (NEB, ref: R3189S) and XhoI (NEB ...
-
bioRxiv - Microbiology 2023Quote: Plasmid DNA containing one copy of the SIV gene was linearized using EcoR1 (New England Biolabs, Ipswich, MA) following their restriction digest protocol (New England BioLabs ...
-
bioRxiv - Genomics 2023Quote: ... Approximately 7,500 cells were diluted with 1.25 ml of a dilution buffer containing 0.4x NEBuffer 2 (NEB, B7002S), 2 mg/ml BSA (ThermoFisher Scientific ...
-
bioRxiv - Developmental Biology 2023Quote: ... and beads resuspended in a 50-µl PCR mix containing 1x NEBNext High-Fidelity PCR Master Mix (NEB) and 0.5 µM of Nextera Ad1_noMX and Ad2.X primers ...
-
bioRxiv - Physiology 2023Quote: ... The PCR was performed in a total volume of 50 µl containing 1× HF buffer (New England BioLabs), 1 µl dNTP Mix (New England BioLabs) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Such probes were generated by linearizing pDONR221-vectors containing the DsuzIR62a-coding sequences using NheI (New England Biolabs) following protocols described above ...
-
Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... The wash solution was then replaced with 1× T4 polymerase buffer containing 30 U T4 DNA polymerase (NEB) and incubated at 12 °C for 30 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... coli in vitro transcription were synthesized through a 200 µL PCR reaction containing 10x Taq Buffer (NEB, #B9014S), 250 µM dNTP Mix (NEB ...
-
bioRxiv - Biophysics 2023Quote: A Tandem HaloTag plasmid containing the HaloTag-GTGSGSGS-HaloTag cDNA sequence was built via Gibson Assembly (NEB, (62)) of two HaloTag genes ...
-
bioRxiv - Bioengineering 2023Quote: ... cells were resuspended in Rnase H reaction buffer containing 20 U/mL of Rnase H enzyme (NEB M0297S). Cells were incubated for one hour at 37 °C with gentle agitation ...
-
bioRxiv - Cell Biology 2024Quote: ... washed and incubated with T cell medium containing 5 μM (Snap-Cell Block S9106S, New England Biolabs, NEB) for 30 min at 37°C ...
-
bioRxiv - Cell Biology 2024Quote: ... washed and incubated with T cell medium containing 5 μM (Snap-Cell Block S9106S, New England Biolabs, NEB) for 30 min at 37°C ...
-
bioRxiv - Microbiology 2024Quote: ... TAP-PCR was performed in a total volume of 25 μL containing 0.25 μL of Q5 polymerase (NEB), 5 μL of GC Enhancer (NEB) ...
-
bioRxiv - Microbiology 2023Quote: NINL (1-702) containing an amino-terminal HaloTag was expressed in BL-21[DE3] cells (New England Biolabs), which were then grown until OD 0.4-0.6 and induced with 0.1 mM IPTG for 16 hr at 16°C ...
-
bioRxiv - Biochemistry 2023Quote: ... Oligos containing the spacer sequence and Esp3I sticky ends were annealed and phosphorylated using T4 Polynucleotide Kinase (NEB) and purified using the Oligo Clean and Concentrator kit (Zymo Research) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... plasmid (Monarch® PCR mini-prep kit) and gel extraction kits (Monarch® DNA gel extraction kit) were also obtained from NEB and used according to the manufacturer’s protocols ...
-
bioRxiv - Genomics 2019Quote: ... mouse NIH/3T3 and human Jurkat DNA were from NEB (Ipswich, MA, USA). E14 genomic DNA was extracted with a DNeasy Blood and Tissue Kit (QIAGEN ...
-
bioRxiv - Microbiology 2020Quote: ... or anti-mouse IgG (H+L) (DyLight™ 800 4X PEG Conjugate, NEB) were used as the secondary antibodies.
-
bioRxiv - Developmental Biology 2023Quote: Regulatory elements were amplified from mouse genomic DNA with Q5 polymerase (NEB, M0491) using primers listed in Table S8 and cloned into pGL4.24[luc2P/minP] (Promega ...
-
bioRxiv - Cell Biology 2020Quote: ... dCas9-SNAP protein samples were incubated with SNAP-Surface Alexa Fluor 647 (NEB S9136) at 37°C for 30 minutes ...
-
bioRxiv - Immunology 2020Quote: ... The proteins were expressed in BL21 DE3 competent cells (New England Biolabs, Ipswich, MA). Protein purification was undertaken using nickel-affinity chromatography (Qiagen ...
-
bioRxiv - Plant Biology 2019Quote: ... Recombinant proteins were purified using gravity flow columns with amylose resin (New England Biolabs). MBP cleavage was performed by incubation in cleavage buffer (50 mM Trizma-HCl ...
-
bioRxiv - Cancer Biology 2021Quote: ... Precipitated proteins were resuspended in 150 μL of Glycoprotein Denaturing Buffer (New England Biolabs) and incubated at 100 °C for 10 min ...
-
bioRxiv - Cancer Biology 2019Quote: ... reduced and alkylated and proteins were digested with first PNGase F (New England BioLabs), endoproteinase Lys-C (Promega ...