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Citations for New England Biolabs :
851 - 900 of 2640 citations for 4 METHANESULPHONYLBUTAN 2 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... 2 mM ribonucleoside vanadyl complex (New England Biolabs), and 2 mg/ml bovine serum albumin (Jackson ImmunoResearch) ...
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bioRxiv - Genomics 2024Quote: ... and then 2 μl of T7 Endonuclease (NEB) was added ...
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bioRxiv - Genomics 2024Quote: ... and 2 μl of Phi29 DNA Polymerase (NEB). The reaction is incubated for 8 hours at 30°C.
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bioRxiv - Genomics 2024Quote: NEBuffer 2 (New England Biolabs, cat. no. B7002)
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bioRxiv - Molecular Biology 2024Quote: ... 2 μl of 10X RNA ligase buffer (NEB), 2 μl 10 mM ATP ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 µL of λ protein phosphatase (NEB P0753S) was added and incubated at 37°C for 30 minutes and then placed on ice ...
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bioRxiv - Genomics 2022Quote: ... and 10 uL 2% BSA (New England Biolabs) for sample resuspension and dilution prior to 10x Genomics chip loading ...
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bioRxiv - Genomics 2022Quote: ... 10 μL of 2% BSA (New England Biolabs), and 984 μL of nuclease-free water 1xST buffer was prepared by diluting 2x ST with ultrapure water (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL LongAmp Taq DNA Polymerase (NEB, # M0323L), 0.5 µM of each primer (First PCR F ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL LongAmp Taq DNA Polymerase (NEB, # M0323L), 0.5 µM of each primer (Second PCR F UMI ...
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bioRxiv - Microbiology 2023Quote: ... 2 µM PNA,1X KAPA master mix (NEB) and 50 ng of template DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µL 10X NEB Buffer #2 (NEB #B7002S), 5 µL RppH (NEB #M0356S) ...
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bioRxiv - Biochemistry 2023Quote: ... 2 μl Taq DNA ligase (80 U, NEB), and 1 μl T4 DNA Polymerase (1 U ...
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bioRxiv - Biochemistry 2023Quote: ... 2 μl 10x Taq DNA Ligase buffer (NEB), 2 uL dNTP Solution Mix (10 mM stock ...
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bioRxiv - Cell Biology 2023Quote: ... 2 U of beta-agarose I (NEB, M0392), and 5 μL of Maxima H minus reverse transcriptase ...
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bioRxiv - Cell Biology 2023Quote: ... 2 uL of T4 RNA Ligase Buffer (NEB) and 1 uL T4 RNA Ligase 2 (made in-house ...
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bioRxiv - Systems Biology 2023Quote: ... The mix contains 1x NEBuffer-2 (NEB, #B7002S), 10 µM of Randomer (custom DNA oligo ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... 10 µl 2× Gibson Assembly Master Mix (NEB)) ...
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bioRxiv - Bioengineering 2023Quote: ... and mRNA Cap 2’-O-methyltransferase (NEB, M0366S). Then ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µL of Lambda Protein Phosphatase (NEB, P0753S) was added to aliquots 3 and 4 (PPase only) ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µM RNCs or empty 70S ribosomes (NEB) were incubated (30 min ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM vanadyl-ribonucleoside (S1402S, New England Biolabs), and 15% formamide (60345 ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 units of T4 polynucleotide kinase (NEB, M0201S), 0.09 nM dNTPs and 0.045 μg/μL of BSA at 12°C for 30 min ...
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bioRxiv - Immunology 2024Quote: ... 2 uL of Rapid PNGase F Buffer (NEB) was added and incubated at 80°C for 3 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... Then 2 μL 10x RNA ligation buffer (NEB), 0.2 μL 100 mM ATP ...
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bioRxiv - Systems Biology 2024Quote: ... 2 µL of 10X Thermo PCR buffer (NEB), 2 µL of ES-E14 wildtype cDNA template or from 1 µL plasmid pLJM1-EGFP for GFP esiRNAs ...
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bioRxiv - Genomics 2024Quote: ... 2 µL of 10 mM ATP (NEB P0756S), 1 µL of E ...
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bioRxiv - Genetics 2023Quote: ... We added phasing nucleotides as well as overhangs for indexing primers using primer mixtures SL5.F[1-4] and SL5.R[1-4] (NEB Q5 for 20 cycles, Tm 62 °C). We finally added dual indexing primers using the i5 and i7 system from Illumina (NEB Q5 for 20 cycles ...
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bioRxiv - Genetics 2022Quote: ... The gRNA binding regions were checked for presence of single nucleotide polymorphism (SNP) by amplifying the region using One Taq 2X master mix (New England Biolabs, Ipswich, Massachusetts, USA) and subsequent sanger sequencing of the PCR product (primers designed by primer-BLAST ...
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bioRxiv - Molecular Biology 2021Quote: ... One microgram of this PCR product and pKLV-U6gRNA(BbsI)-PGKpuro2ABFP was digested with MluI and BamHI (New England Biolabs, Ipswich, MA, USA). The digested products were purified ...
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bioRxiv - Genomics 2023Quote: ... The strain CBS 411.78-was prepared and sequenced with the ligation kit SQK108 in a one-pot reaction using 500 ng DNA for end prep and ligation (NEB Ultra-II ligase) and sequenced on an R9.4.1 flowcell ...
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bioRxiv - Genomics 2022Quote: ... and one short read library using the NEBNext Ultra II FS DNA Library Kit for Illumina (New England BioLabs Inc., Ipswich, MA, USA). The long-read library was then sequenced on the PacBio Sequel IIe system in CLR mode using the Sequel II Binding Kit 2.2 (PacBio) ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 viral RNA detection and quantification was performed using the Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs, Whitby, ON, Canada) on the Rotor-gene Q platform (Qiagen) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Then total RNA (500 ng per sample) was purified by one round of polyA mRNA selection with oligo-dT magnetic beads (NEB, Cat. No. S1550S), converted to cDNA using KAPA mRNA HyperPrep kit (KAPA biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... falciparum in placenta samples was evaluated using One Taq® Quick-Load® 2X Master Mix with Standard Buffer (NEB, Cat No. M0486L) and the following thermocycler program ...
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bioRxiv - Genomics 2022Quote: ... 2 μl of 10X CutSmart® buffer (1X final) and 2 μl of 1U/μl USER® II enzyme (M5505S, NEB) (0.1U/μl final ...
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bioRxiv - Microbiology 2020Quote: ... stained with a mAb cocktail composed of SARS-CoV-2 spike (Creative-Bios; 2BCE5) and SARS-CoV-2 nucleoprotein (Creative-Biolabs; NP1C7C7) followed by anti-Mouse IgG-HRP (Abcam ab6823 ...
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bioRxiv - Genomics 2021Quote: ... and supernatant was transferred to a new tube and added to 2 μL 10% SDS and 2 μL 20 mg/mL proteinase K (New England Biolabs P8107). Samples were incubated overnight at 65°C to reverse crosslinks ...
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bioRxiv - Bioengineering 2022Quote: ... 1X of a restriction enzyme mix [1/2 EcoRI-HF® (NEB #R3101, 2/5 Nuclease-free water, 1/10 CutSmart Buffer 10X (NEB)] ...
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bioRxiv - Genetics 2023Quote: ... 5 µl of 100 ng/µLcDNA diluted from the previous step was combined with 2 µL of block mix and 2 µL of nuclease free water (NEB, AM9937), and then the cDNA block oligo mix was incubated on a thermocycler under the following conditions to allow block oligo mix to bind to the 5′ end and the 3′ end of the cDNA molecule ...
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bioRxiv - Microbiology 2023Quote: ... antibiotic resistance cassette and 2 flanking regions were combined in equimolar amounts and mixed with 2 x HiFi reagent (NEB, UK) and incubated at 50°C for 1 hour ...
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bioRxiv - Synthetic Biology 2021Quote: ... for 4 hours at 37°C and treated with Antarctic phosphatase (NEB) for 30 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Annealed oligos were mixed with 4 μL 6X loading dye (NEB, B7024S), loaded onto a lab-made 8% TBE gel (Acrylamide/Bis solution 37.5:1 ...
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bioRxiv - Genomics 2020Quote: ... the circularized DNA was incubated in 1x NEBuffer 4 reaction buffer (NEB) (50 mM potassium acetate ...
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bioRxiv - Genomics 2019Quote: ... 4) We used 2.75 µl 10X RNA Fragmentation Buffer (New England Biolabs) in a total 27.5 µl fragmentation reaction volume and later on added 2.75 µl 10x RNA Fragmentation Stop Solution ...
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bioRxiv - Molecular Biology 2019Quote: ... Oligonucleotides (1) and (4) were phosphorylated with T4 PNK (New England Biolabs) to allow ligation to the backbone fragment ...
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bioRxiv - Systems Biology 2020Quote: ... a 4-cycle PCR was performed with OneTaq polymerase (New England Biolabs) in 200 reactions (125 μL/reaction) ...
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bioRxiv - Microbiology 2021Quote: ... The parental plasmid was digested for 4 h using Dpn1 endonuclease (NEB) at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... a mix of 4 μl First strand Synthesis Reaction Buffer (NEB-kit), 0.5 μl Murine RNase Inhibitor (NEB-kit ...
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bioRxiv - Genomics 2020Quote: ... 4 μL of 20 mg/mL RNase A (New England Biolabs #T3018L) is added to the RNase positive aliquot of non-lysed TSB from 1-gram cannabis flower homogenate ...