Labshake search
Citations for New England Biolabs :
851 - 900 of 5197 citations for 4 2 Methylimidazol 1 ylmethyl phenylamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... (2) +28 μl Dam at 8 U/μl (NEB M0222L); (3 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2 U of DNase I-XT (New England Biolabs, USA) was added into 10 μL of reaction mixture and incubation was extended for 30 min at 37°C to remove the DNA templates ...
-
Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... we performed 2 reactions per sample (20 µl 5× NEB Q5 buffer ...
-
bioRxiv - Genomics 2023Quote: ... The APOBEC reaction mix (2 μL APOBEC reaction buffer (NEB), 0.4 μL APOBEC (NEB) ...
-
Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: ... and samples were then individually diluted in NEBuffer 2 (NEB) and fragmented by incubating at 92 °C for 1.5 min ...
-
bioRxiv - Genomics 2023Quote: ... The APOBEC reaction mix (2 μL APOBEC reaction buffer (NEB), 0.2 μL APOBEC (NEB) ...
-
bioRxiv - Genomics 2022Quote: ... 2 U/μl Thermolabile Exonuclease I (NEB, cat. no. M0568), 50 mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2022Quote: ... 2-3 ×106 cells were treated with MNase (NEB M0247) ranging from 10-30 Kunitz U in 800 μl of custom buffer (Tris-HCl pH 7.5 10 mM ...
-
bioRxiv - Genetics 2022Quote: ... and 2 μL of lambda protein phosphatase (NEB, Catalog #P0753S) were added to the washed FRQ-coupled V5 resin ...
-
bioRxiv - Microbiology 2022Quote: ... (2 µg enzyme in 100 µL H2O) (New England Biolabs) at 37 °C overnight ...
-
bioRxiv - Synthetic Biology 2022Quote: ... and 2 units of Phusion High-Fidelity DNA Polymerase (NEB) in HF Phusion buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... were expressed in Rosetta 2 (DE3) pLysS competent cells (NEB) grown in modified Terrific Broth at 27°C for 7 hours ...
-
bioRxiv - Genomics 2023Quote: ... 2 μL of Monarch RNase A (New England Biolabs (NEB)) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 2 U/mL DNase I (New England Biolabs, M0303L). Cells were passed through a 40 µm mash and seeded at high density in BME.
-
bioRxiv - Evolutionary Biology 2024Quote: ... Cas12a RNP: (13 µl water; 2 µl r2.1 buffer [NEB, Cat ...
-
bioRxiv - Systems Biology 2024Quote: ... and 2 μL of T4 PNK (10 U/μL, NEB) were added ...
-
bioRxiv - Genomics 2024Quote: ... was added to the gDNA in 1x NEBuffer 2 (NEB) in a final volume of 25 µL and incubated for 30-min at 37°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2 µl T7 RNA Polymerase (New England BioLabs, Ipswich, MA), 5 µl ATP (10 μmol) ...
-
bioRxiv - Systems Biology 2024Quote: ... 2 μL of T4 DNA Polymerase (3 U/μL, NEB) 1 μL of Klenow DNA Polymerase (5 U/μL ...
-
bioRxiv - Systems Biology 2024Quote: ... and 2 μL of DNA Polymerase I (10U/μL, NEB) were added and mixed by pipetting ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and 2 units of Phusion High-Fidelity DNA Polymerase (NEB) in HF Phusion buffer ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 2 µl of RNase H Buffer (New England Biolabs) was added and incubated at 45°C for 30 min ...
-
bioRxiv - Microbiology 2024Quote: ... lysates were treated with 2 units of Proteinase K (NEB) for 30min ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... 2 µL of 20 µM SpCas9 (New England Biolabs M0646T) were gently mixed with 2 µL of 100 µM gRNA ...
-
High-resolution profiling reveals coupled transcriptional and translational regulation of transgenesbioRxiv - Synthetic Biology 2024Quote: ... treated with 2 µL DNase I (New England Biolabs, M0303), and incubated at 37°C for 30 min to degrade the IVT PCR template DNA ...
-
bioRxiv - Microbiology 2024Quote: ... burgdorferi cells using QuickLoad 2× Taq Master Mix (NEB, M0271). Positive clones were flash frozen on dry ice in BSK-II containing 10% DMSO (Sigma ...
-
bioRxiv - Biochemistry 2024Quote: ... 2 units of either DNase I or T7 Exonuclease (NEB) were then added ...
-
bioRxiv - Genomics 2024Quote: ... and 50 μL of NEBNext 2× Master Mix (NEB M0541S). oGH840.1-6 are six oligonucleotides with variable lengths to stagger and phase the sequencing of a common sequence in the target site amplicon ...
-
bioRxiv - Genetics 2024Quote: ... and digested with 2 μl of β-agarase I (NEB) at 42 °C for 90 min ...
-
bioRxiv - Genetics 2024Quote: ... 2 μL of T4 Ligase buffer (New England Biolabs, USA) and 1 μL of T4 Polynucleotide Kinase (10 U/μL ...
-
bioRxiv - Biophysics 2024Quote: ... before annealing and ligated using T4 RNA ligase 2 (NEB). Constructs containing 30 nt gaps ...
-
bioRxiv - Bioengineering 2024Quote: ... 2 μL of T4 DNA ligase (NEB, 400000 U/mL), and 4 μL of 10X T4 DNA ligase buffer (NEB) ...
-
bioRxiv - Genetics 2024Quote: ... using NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541S). We inserted these three fragments into the pLS-SceI plasmid (Addgene ...
-
bioRxiv - Developmental Biology 2024Quote: ... together with 2 µM of Cas9 protein (NEB, Cat# M0646T). Tol2-plasmids were diluted in 0.05% Phenol red to a final concentration of 100 ng/µL ...
-
bioRxiv - Plant Biology 2020Quote: ... 19 μL binding mixture containing 4 μL nuclear extracts and 5U Rnase H (NEB) or bovine pancreatic RNase A (Qiagen ...
-
bioRxiv - Cancer Biology 2020Quote: RNA 5’-ends were phosphorylated using 4 µl T4 PNK (NEB, catalog no. M0201) in a solution consisting of 8 µl 10x PNK buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... and 10 pmol of each used in a 4-fragment Gibson assembly reaction (NEB) to generate GT10 5’_pSY45_pDS66_GT10cHA3 Flox_GT10 3’_pUC19 ...
-
bioRxiv - Cell Biology 2021Quote: ... the supernatant fraction was incubated with 4 ml amylose resin (New England Biolabs, E8021L) for 1 h ...
-
bioRxiv - Microbiology 2023Quote: ... gene marker cassette by overnight ligation at 4 °C with T4 DNA Ligase (NEB). Overnight ligations were transformed into chemically competent DH5ɑ E ...
-
bioRxiv - Microbiology 2023Quote: ... Ligation was performed overnight at 4 °C using T4 DNA ligase (New England Biolabs) followed by heat-inactivation for 10 min at 80 °C ...
-
bioRxiv - Genomics 2023Quote: ... and 30% for overnight at 4 °C) with RNase inhibitor [New England Biolabs (NEB), M0314L ...
-
bioRxiv - Microbiology 2023Quote: ... 4 μL template DNA and 0.4 units of Phusion High-Fidelity DNA Polymerase (NEB) (primer sequences provided in Table S3) ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 10 pmol of each used in a 4-fragment Gibson assembly reaction (NEB) to generate TbGT8 5’_BSDr_TbGT8 3’_pUC19 ...
-
bioRxiv - Genetics 2024Quote: ... 120 μL (4 mg/mL) streptavidin magnetic beads (NEB cat no. 50-812-660) were washed 3 times with 1 mL of lysis buffer then resuspended in 100 μL of complete lysis buffer and added to the hybridization mix and then incubated at 37 °C for an additional 30 min with mixing ...
-
bioRxiv - Developmental Biology 2023Quote: ... ∞ at 4 °C) using NEBNext HighFidelty 2x PCR Master Mix (New England Biolabs, M0541S) and Illumina i5 and i7 indices54 ...
-
bioRxiv - Genomics 2022Quote: ... then 4 μl of Q5 High-Fidelity 2X Master Mix (NEB, cat. no. M0541L) was added to each well ...
-
bioRxiv - Genomics 2022Quote: ... the DNA was added to 4.5 μL of 10X NEBuffer 4 (New England Biolabs), uridine diphosphate-6-azideglucose (UDP-6-N3-Glu ...
-
bioRxiv - Biochemistry 2023Quote: ... activation was performed overnight at 4 °C with 5 μg of Factor Xa (NEB) per mg of protein ...
-
bioRxiv - Genomics 2022Quote: ... 16 µl RNA were mixed with 4 µl LunaScript master mix (NEB cat# E3010L), according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... Fragmented gDNA (4 μg) was digested with 20 U of RNase H (NEB #M0297S) for 6 hours to serve as a negative control ...