Labshake search
Citations for New England Biolabs :
8801 - 8850 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... cDNA was synthesized using the ProtoScript II Reverse Transcriptase kit (New England BioLabs, Évry-Courcouronnes, France). qRT-PCR was performed in quadruplets on a CFX384 Touch Real-Time PCR Detection System (Biorad ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2024Quote: ... The SUGCT insert and pcDNA5/FRT vector were ligated by using the Quick Ligation Kit (NEB) and transformed to TOP10 chemically competent cells (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... and then used as input for the NEBNext Ultra II RNA Library Prep Kit (NEB #E7770S). Sample preparation was performed as per manufacturer’s specifications ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A genomic library was prepared using the NEBNext Ultra DNA Library Prep Kit (New England BioLabs) and sequenced on an Illumina NovaSeq (S4 Flow Cell ...
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bioRxiv - Microbiology 2024Quote: ... and libraries for Illumina sequencing were performed using the NEBNext Ultra II RNA kit (NEB, #E7770L). Quantification of the library was performed using dsDNA HS Assay Kit and Qubit 2.0 (Molecular Probes ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA depletion was performed using the NEBNext rRNA Depletion Kit for Bacteria (New England Biolabs), spiked with a custom oligo pool ...
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bioRxiv - Molecular Biology 2024Quote: ... while library concentration was determined using the NEBNext Library Quant Kit for Illumina (NEB, Cat# E7630). All input RNA had RNA integrity number (RIN ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg of total RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs) according to the manufacturer’s instructions and 1 µl cDNA was used as a template for a 10 µl qPCR reaction ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of RNA was used for cDNA synthesis using the LunaScript RT SuperMix Kit (NEB) with and without reverse-transcriptase ...
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bioRxiv - Neuroscience 2024Quote: Libraries for sequencing were prepared using the NEBNext Ultra II library prep kit (New England Biolabs) following the protocol and thermocycling temperatures therein ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized from RNA using ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs) using random primers (included with kit) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... followed by processing with the NEBNext Ultra Directional RNA Sequencing for Illumina kit (New England Biolabs). Illumina sequencing ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription was performed using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB, #E6560S) and 18-mer oligo dT primers ...
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bioRxiv - Molecular Biology 2024Quote: ... The dsDNA was then linearly amplified using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) for 15Lh at 37L°C ...
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bioRxiv - Molecular Biology 2024Quote: ... were used to generate gRNA using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB) and purified using the RNeasy Mini Kit (Qiagen).
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bioRxiv - Microbiology 2024Quote: ... thermophilus extracts or PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, Ipswich, MA, USA) and the reactions were further incubated at either 50 °C or 37 °C for another 4 h in the case of T ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA extraction was done using a Monarch® Total RNA Miniprep Kit (New England Biolabs) prior to treatment with TURBO DNAse (Ambion) ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from cells using the Monarch® Total RNA Miniprep Kit (New England Biolabs and 200ng of RNA was converted to complementary DNA (cDNA ...
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Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: ... Illumina sequencing adaptors were ligated onto the amplicons using the NEBNext Adaptor kit for Illumina (NEB). The amplicons were then pooled ...
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bioRxiv - Biophysics 2024Quote: ... and the resulting RNAs were isolated from the reaction mixtures by Monarch RNA Cleanup Kit (NEB). The size and purity of the RNA constructs was confirmed by TBE-urea PAGE (Supplementary Fig ...
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bioRxiv - Microbiology 2024Quote: ... Barcodes (Native Barcoding Kit 96 (SQK-NBD109.96)) were ligated using Blunt/TA Ligase Master Mil (NEB). Barcoded libraries were purified using KAPA Pure Beads (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... Ribosomal RNA (rRNA) was then depleted using the NEBNext rRNA Depletion Kit for Bacteria (NEB, #E7850) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Genetics 2023Quote: ... libraries were prepared according to instructions in the NEBNext small RNA sequencing kit (New England Biolabs) or Nextflex Small RNA-Seq Kit v3 (Perkin Elmer) ...
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bioRxiv - Developmental Biology 2024Quote: ... Poly(A) mRNA selection and library preparation was performed using NEBNext Ultra II RNA Kit (NEB). Sequencing was performed at Boston University Microarray and Sequencing core ...
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bioRxiv - Microbiology 2023Quote: ... Subtraction of both bacterial and mammalian rRNA was done using the NEBNext rRNA Depletion Kits (NEB), and sequenced on a Novaseq 6000 instrument (Novogene) ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from 200 ng DNA using the NEB Enzymatic Methyl-Seq kit (NEB #E7120) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... site-directed mutagenesis was done using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs) as directed by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... cloned into the pZE21 expression vector via Gibson cloning (NEBuilder HiFi DNA Assembly Kit from NEB), and then transformed into E ...
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bioRxiv - Genomics 2024Quote: Genomic DNA was extracted from each fraction using a Monarch Genomic DNA Purification Kit (NEB #T3010). Barcode sequences were amplified from gDNA using a two-step protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit (NEB #E7645L) with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Sets 1 and 2 ...
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bioRxiv - Immunology 2024Quote: ... cDNA synthesis was performed using ProtoScript M-MuLV First Strand cDNA synthesis kit (New England Biolabs) and random hexamer primers ...
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bioRxiv - Genomics 2024Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) for Illumina according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... libraries were prepared using the NEBNext Ultra II Directional RNA kit (New England BioLabs, Ipswich, MA) and sequenced with an NovaSeq 6000 at the Vanderbilt University Medical Center genomics core (VANTAGE).
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bioRxiv - Genomics 2024Quote: ... The library was generated using an NEB Ultra II DNA Library Prep kit (NEB, Ipswich, MA) with an Illumina-compatible y-adaptor ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using a Monarch® HMW DNA Extraction Kit for Tissue (New England Biolabs) and the supplied protocol for yeast ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Sequencing libraries were generated using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA), following the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2024Quote: All gene insertions were carried out using the NEBuilder HiFi DNA Assembly kit (New England Biolabs).
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bioRxiv - Biophysics 2024Quote: ... Plasmids created in this study were assembled using the Gibson Assembly Cloning Kit (New England Biolabs). All plasmid sequences were confirmed by whole plasmid sequences services provided my Plasmidsaurus SNPsaurus LLC.
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were prepared using the NEBNext small RNA library prep kit (New England Biolabs E7330) and sequenced by Novaseq PE150 ...
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bioRxiv - Cancer Biology 2024Quote: Libraries were prepared on beads using the NEBNext Ultra II DNA Library Prep Kit (NEB, #E7645). First ...
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bioRxiv - Cell Biology 2024Quote: ... Site directed mutagenesis was used to create an additional Lv165 BMI1 overexpression vectors with a P2A sequence instead of the IRES by designing primers using the New England Biolabs (NEB)-base changer website (https://nebasechanger.neb.com) in combination with the Q5 Site-Directed Mutagenesis kit (NEB, E0554S), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... We quantified the libraries using the NEBNext Library Quant Kit for Illumina (New England Biolabs, #E7630) based on the mean insert size provided by the Bioanalyzer ...
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bioRxiv - Neuroscience 2023Quote: ... The K262E point mutation was introduced with the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). pSecTag2A was used for control experiments ...
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bioRxiv - Immunology 2023Quote: ... Library preparation was done using NEBNext Ultra RNA Library Prep Kit (New England Biolabs, Ipswich, USA) following manufacturer recommendations ...
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bioRxiv - Molecular Biology 2023Quote: 75ng of the amplified library was used as input for an RNA Synthesis Kit (NEB, E2050S) and concentrated using a Monarch RNA clean-up kit (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Illumina libraries were built using Ultra II FS DNA Library Prep Kit (New England Biolabs, USA) for Illumina and sequenced on an Nextseq500 platform to generate 150 bp paired-end reads ...
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bioRxiv - Developmental Biology 2023Quote: ... Library preparation was performed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645S) according to the manual of the kit ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... reverse transcription from mRNA was performed using the LunaScript® RT SuperMix Kit (New England BioLabs) following standard protocols ...
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bioRxiv - Microbiology 2023Quote: ... Site-directed mutations were introduced using the Q5® site-directed mutagenesis kit (New England BioLabs) as per the manufacturer’s protocol ...