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Citations for New England Biolabs :
801 - 850 of 10000+ citations for rno mir 137 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: The PCR products were cleaned using the Monarch® DNA Gel Extraction Kit (NEB), and 3’ end A-overhangs were added by NEBNext® dA-Tailing Module (NEB) ...
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bioRxiv - Genetics 2022Quote: ... PCR products were extracted with the Monarch DNA Gel Extraction Kit (New England Biolabs) after running on an agarose gel ...
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bioRxiv - Biophysics 2019Quote: ... The samples were then purified with the Monarch® PCR & DNA Cleanup Kit (NEB) and eluted in 10 μl ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA was purified by using a Monarch® PCR&DNA cleanup kit (NEB #T1030) before analyzing the DNA by capillary electrophoresis (30) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The PCR products were later purified with the Monarch Purification Kit (NewEngland Biolabs, #T1030S) prior to deep sequencing analysis ...
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bioRxiv - Biophysics 2021Quote: ... Mutants were generated by mutagenesis PCR using the Q5 Site-Directed Mutagenesis Kit (NEB), according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... The two PCR products were ligated together by the Gibson assembly cloning kit (NEB)
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bioRxiv - Microbiology 2022Quote: ... cDNA was purified using the Monarch® PCR & DNA Cleanup Kit (NEB, cat# T1030). PCR was performed using either KAPA HIFI 2X ready mix (KAPA Biosystem ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by cleaning up with Monarch PCR & DNA Cleanup Kit (T1030, New England Biolabs). The ligated DNA was repaired with PreCR Repair Mix (M0309 ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR product obtained were purified using the Monarch DNA Gel Extraction Kit (NEB) and cloned into the pDONR207 using a BP Clonase II Kit (Thermo Fisher) ...
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bioRxiv - Plant Biology 2022Quote: ... We cloned each fragment into pMiniT using the PCR Cloning Kit (New England BioLabs), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The product was purified with a Monarch PCR and DNA cleanup kit (NEB, T1030) and restricted with enzymes Nde1 (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... The full-length PCR product was purified using Monarch Gel Extraction Kit (NEB #T1020), according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified using the Monarch PCR and DNA Clean Up Kit (#T1030L, NEB), Illumina NGS libraries were prepared using the NEBNext Ultra II DNA Kit (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified using the Monarch PCR and DNA Clean Up Kit (#T1030L, NEB) and ChIP-seq libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (E7546S ...
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bioRxiv - Microbiology 2023Quote: ... used for Polymerase Chain Reaction (PCR) and Golden gate cloning kit (New England Biolabs) were carried out according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... cleaned and concentrated 20x using the Monarch PCR & DNA Cleanup Kit (5 µg) (NEB). Further cleanup was done with the E-gel imager system (Invitrogen ...
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bioRxiv - Systems Biology 2023Quote: ... The tagmentation process was promptly neutralised using the Monarch PCR & DNA Cleanup Kit (NEB). The samples were then eluted in a final volume of 20 μL using the Elution buffer.
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bioRxiv - Plant Biology 2023Quote: ... desalted using GenepHlow PCR Cleanup Kit (GeneAid) and ligated with T4 DNA ligase (NEB), each step according to manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was used as a template for PCR (Phusion HF kit (New England Biolabs)) with primers designed to amplify the spike and HE genes (Table 1) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the cDNA libraries were purified using the Monarch PCR & DNA Cleanup Kit (NEB). High-throughput sequencing was performed on an Illumina NextSeq 550 in paired-end mode with 150 cycles per end.
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bioRxiv - Biophysics 2023Quote: ... the mixture was purified using the Monarch PCR and plasmid DNA purification kit (NEB), eluted in 10 mM Tris-HCl pH8.0 and stored at -20 °C for further experiments ...
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bioRxiv - Molecular Biology 2023Quote: ... and then purified with a Monarch PCR & DNA Cleanup kit (New England Biolabs, USA). For IVT reactions ...
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bioRxiv - Genetics 2023Quote: ... and introduced mutations using a Q5 Site-Directed PCR Mutagenesis Kit (New England Biolabs). After mutagenesis ...
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bioRxiv - Systems Biology 2024Quote: ... the enzyme’s activity was immediately neutralised using the Monarch PCR & DNA Cleanup Kit (NEB). Subsequently ...
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bioRxiv - Biochemistry 2024Quote: All PCR was performed with Q5 High-Fidelity DNA Polymerase kit (New England Biolabs) according to manufacturer’s protocols ...
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bioRxiv - Genomics 2024Quote: The ligation product was purified using a Monarch PCR & DNA Cleanup Kit (NEB #T1030) following the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2024Quote: ... The PCR fragments were joined together using Gibson assembly kit (NEB Cat. No. E2611L) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Mutants selected for cloning were also analysed via T7 endonuclease I-based (T7E1) heteroduplex assay according to the manufacturers protocol (EnGen® Mutation Detection Kit, NEB #E3321), and the length of digested and undigested fragments were visually compared by gel electrophoresis (Fig ...
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bioRxiv - Neuroscience 2020Quote: ... as well as wildtype (WT) N2aC were assayed for mis-matched DNA pairs via T7 endonuclease assay (EnGen Mutation Detection Kit; New England Biolabs, Ipswich, MA) with PCR primers targeted towards our genomic region of interest (Integrated DNA technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... We designed spCas9 guide RNAs to target codon 12 of the human KRAS gene and selected the one with highest targeting efficiency determined by T7E1 mismatch assay (EnGen Mutation Detection Kit, New England Biolabs, Ipswich, MA). Truncated guide RNA was produced by PCR assembly of a guide RNA template (56 ...
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bioRxiv - Microbiology 2020Quote: ... bacilliformis total RNA (for each 25 µl reaction) collected from various conditions using the Luna Universal One-Step RT-qPCR kit (New England BioLabs; Ipswich, MA) according to the manufacturer ...
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bioRxiv - Microbiology 2019Quote: ... and also quantified using NEB Luna Universal qPCR Master Mix and Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA) for qPCR and RT-qPCR test ...
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bioRxiv - Plant Biology 2019Quote: ... Three different biological replicates represented by total RNA extracted from three individual plants and reverse transcribed into cDNA by LunaScript®RT SuperMix Kit (New England Biolabs Inc.) were used for statistical analysis of the quantification ...
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bioRxiv - Microbiology 2021Quote: ... nucleic acid (5 μL) was used as template for reverse transcription using LunaScript® RT SuperMix Kit (New England Biolabs, Hitchin, UK) in 20 μL reaction volume ...
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bioRxiv - Developmental Biology 2022Quote: ... <200nt isolates were immediately frozen at -80⍰°C while >200nt isolates were used as template for cDNA synthesis (LunaScript RT Mastermix Kit, New England Biolabs, MA, USA) and subsequent doublesex sexing PCR as previously described (25) ...
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bioRxiv - Cell Biology 2022Quote: ... RNA integrity was validated by gel electrophoresis and 800 ng were reverse transcribed using the LunaScript® RT SuperMix Kit according to manufacturer’s instructions (E3010, New England Biolabs, Ipswich, MA). Real-time PCR was performed using the Blue ŚGreen qPCR Kit (F416 ...
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bioRxiv - Microbiology 2020Quote: ... Five hundred nanograms total RNA was then reverse-transcribed in 20 μl reaction volume using the LunaScript RT SuperMix Kit (E3010, New England Biolabs, MA, USA). Quantitative PCR was performed by transferring 2 μl of the RT mix to the qPCR mix prepared with Luna Universal qPCR Master Mix (M3003 ...
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bioRxiv - Microbiology 2019Quote: ... Measurement of differential gene expression was conducted using a qTower 2.2 (Analytik Jena, Jena, Germany) with the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswitch, USA). Primer pairs used for the analysis are listed in Table S3 D ...
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bioRxiv - Immunology 2022Quote: ... as described by Corman et al84 were used alongside the Luna Universal One-Step RT-qPCR kit (New England Biolabs, Ipswitch, MA) and CFX384 Touch Real-Time System (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... 150 ng of total RNA per sample was used to synthesize cDNA using LunaScript RT SuperMix kit (Cat# E3010L, NewEngland BioLabs, MA, USA) in a 20 µl reaction volume ...
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bioRxiv - Genetics 2021Quote: ... The entire alh-6 genomic sequence (ATG to stop) was amplified by PCR and cloned in a linearized pMiniT 2.0 vector (NEB PCR Cloning Kit, Cat. #E1202S). Plasmid DNA was purified using the Zymo Research Zyppy Plasmid Miniprep kit (Cat ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The library was then PCR amplified in five separate aliquots (12 cycles, Phusion High Fidelity® PCR kit, New England Biolabs Inc., Frankfurt, Germany). We used Agencourt® AMPure XP beads (Beckman Coulter ...
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bioRxiv - Genetics 2022Quote: ... DNA samples were purified before and after PCR with the Monarch® PCR and DNA Clean-Up Kit (New England Biolabs, Ipswitch, MA, USA). Individual sample libraries were pooled ...
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bioRxiv - Systems Biology 2022Quote: ... Unused RT primers were digested using Exonuclease I (NEB) and wells were pooled by half plates ...
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Har-P, a short P-element variant, weaponizes P-transposase to severely impair Drosophila developmentbioRxiv - Molecular Biology 2019Quote: ... 3µg was reverse transcribed using Protoscript RT enzyme (NEB) as per manufacturer’s protocol and negative RT control was carried out similarly without RT enzyme ...
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bioRxiv - Immunology 2022Quote: ... 1 μl 20X Luna WarmStart RT Enzyme Mix (NEB) and 10 μl 2X Luna Universal One-step Reaction mix (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... The RT reaction was performed using the Luna (NEB). The cDNA was amplified in a 25 µl PCR reactions using 6 µl cDNA and 2X Master mix (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... and LunaScript® RT SuperMix (New England Biolabs, USA), referred to as Superscript™ and LunaScript® ...
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bioRxiv - Genomics 2023Quote: ... 8 μL of 5X Induro RT Buffer (NEB M0681S), 12.6 μL nuclease free water ...