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Citations for New England Biolabs :
801 - 850 of 1077 citations for Recombinant Swine CCL19 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 5 µg antibody (Table. S3) and 50 µl protein A magnetic beads (New England Biolabs, Cat. No. S1425S) were added to the supernatant and incubate overnight at 4 degree Celsius ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 40 μl of filtered double-distilled water and 20 μl of Blue Protein Loading Dye (New England Biolabs) were added to the beads ...
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bioRxiv - Molecular Biology 2020Quote: ... σA-FLAG and RbpA-FLAG proteins were prepared by PCR using Q5® High-Fidelity DNA Polymerase (NEB) with primers #3130 + #3131 (HelD) ...
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bioRxiv - Biochemistry 2021Quote: ... His tagged proteins were eluted with lysis buffer containing 400mM imidazole and bound immediately to amylose resin (NEB) for 1hr ...
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bioRxiv - Microbiology 2021Quote: ... Standard curve was prepared using SARS-CoV-2 Positive Control plasmid containing full nucleocapsid protein (N gene) (NEB) and used to quantify copies of N gene in organoid samples ...
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bioRxiv - Microbiology 2021Quote: ... A small fraction of the refolded MBP-ACE2NTD fusion protein was further purified by amylose magnetic beads (NEB) according to the protocol provided by the manufacturer.
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bioRxiv - Microbiology 2022Quote: ... Either 3-5 µL of Color Prestained Protein Standard-Broad Range (11-245 kDa) (New England Biolabs, P7712) or 1-3 µL of PageRuler Prestained Protein Ladder (10-180 kDa ...
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bioRxiv - Microbiology 2022Quote: ... 1-20 μg of the Env protein was mixed with 1 μl of Glycoprotein Denaturing Buffer (NEB, 10×) and H2O (if necessary ...
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bioRxiv - Cell Biology 2022Quote: ... residual ATP from the protein preparation was depleted using apyrase by mixing 4.5 µl of 500 nM protein with a 0.5 µl of apyrase (NEB), and incubating for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: Cell-free transcription and translation reactions were carried out using PURExpress® In Vitro Protein Synthesis Kit (NEB) following the manufactureŕs protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... the Gbait and dld gRNAs were combined at 4.8uM each with 20uM Lba Cas12a protein (New England Biolabs) and incubated for 10 minutes at 370C ...
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bioRxiv - Genetics 2023Quote: N-terminal GST-fusion TF DBD proteins were expressed using the PURExpress in vitro transcription/translation kit (NEB) following the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2023Quote: ... 0.27 nmol ErCas12a/LbCas12aU protein and 0.45 nmol crRNA were assembled in 1 X Nuclease Reaction Buffer (NEB). The protein and RNA were mixed and incubated for 10 minutes at room temperature and used for transformation of embryogenic C ...
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bioRxiv - Biochemistry 2023Quote: ... and then deglycosylated and dephosphorylated overnight at 37 °C with the protein deglycosylation mix II (New England Biolabs) and Quick CIP (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... samples containing the same amount of protein (20 µg/sample) were processed for endoglycosidase-H (Endo-H, NEB) digestion using 5 milliunits Endo-H for 16h at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... Selected candidates were expressed in vitro using the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were expressed and purified from 2 to 4 liters of Escherichia coli NiCo21(DE3) (New England Biolabs) as previously described (24) ...
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bioRxiv - Genetics 2023Quote: Total protein extracts were prepared with 2.106 cells re-suspended in 50 μL of loading buffer (B7709S, Biolabs) containing 1% SDS ...
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bioRxiv - Microbiology 2023Quote: ... E protein glycosylation status was determined by treating RVP lysates with PNGase F (Cat#P0704S, New England Biolabs) for 3 hours at 37°C ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were purified using the amylose resin affinity purification method by following the manufacturer’s recommendations (E8021L, NEB). Briefly ...
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bioRxiv - Neuroscience 2024Quote: HA-tagged BirA-Tau fusion proteins were generated by using the NEBuilder™ HiFi DNA Assembly Kit (NEB). To this end ...
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bioRxiv - Microbiology 2024Quote: ... Purified 6xHis-TEV-PK was supplemented with 10 mM MnCl2 and 4,000 U of lambda protein phosphatase (NEB), and incubated at 30°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Ribosome nascent chain complexes were generated using the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs, E6800S). A 100 µL reaction was used ...
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bioRxiv - Molecular Biology 2024Quote: ... Ribosome/nascent chain complexes (RNCs) were generated with a PURExpress In Vitro Protein Synthesis Kit (New England Biolabs, E6800S ...
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bioRxiv - Immunology 2024Quote: The puromycin-conjugated mRNA templates were translated using a PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) with the addition of PURExpress Disulfide Bond Enhancer (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... MBP tagged protein was subsequently bound in batch for 1 h to 1 ml of Amylose resin (NEB) per litre of input culture (typically 4 ml total resin) ...
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bioRxiv - Molecular Biology 2024Quote: CD52 - Fc fusion protein (20µg) was cleaved with 2 µL of FXa protease (New England Biolabs, Ipswich, USA) in a volume of 350 µL of water containing 2 mM of CaCl2 ...
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bioRxiv - Microbiology 2024Quote: ... cell-free transcription-translation assays were carried out using the PURExpress® In Vitro Protein Synthesis Kit (NEB) following the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2020Quote: ... 40 µl of the cleared lysates were incubated with 1 µl Lambda Protein Phosphatase (λ phosphatase) (New England Biolabs), 5 µl 10x buffer for Protein MetalloPhosphatases (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... The reactions were stopped by adding EDTA and the proteins were digested by adding Proteinase K (New England Biolabs) at 37℃ for 20min ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mg of cell lysate was cleared with protein A magnetic beads to remove non-specific interactions (S1425S, NEB) and incubated overnight with anti-ZAP antibody (Proteintech 16820-1-AP ...
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bioRxiv - Molecular Biology 2020Quote: ... 1600 v/10 ms /3 pulses for 200,000 cells in Buffer R (Neon Transfection kit) premixed with 50 pmol Cas9 protein (CAT#M0646T, New England Biolabs), 50 pmol single guide RNA (sgRNA ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 1.5 mg of protein was cleaved in a 2 ml reaction with 240 Units of TEV protease (NEB) for two hours at 30 °C ...
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bioRxiv - Physiology 2021Quote: ... The biotinylated peptides were placed on streptavidin-coated plates and three rounds of solid-phase panning were conducted using 109 random 12-mer peptides fused to a minor coat protein of M13 (pIII) following the manufacture’s protocol (Ph.D-12 Phage Display Peptide Library Kit, NEB, E8110S). Sequenced data were subsequently analyzed as follows ...
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bioRxiv - Bioengineering 2021Quote: ... and cleavage of the fusion protein with enterokinase that were performed according to manufacturer’s instructions (amylose resin purification – according to pMAL Protein Fusion & Purification System, New England Biolabs, Ltd. ...
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bioRxiv - Biochemistry 2021Quote: ... 500 μg of “heavy” or “light” THP-1 protein extract were dephosphorylated with 50 U of Antarctic phosphatase (NEB) in Antarctic phosphatase buffer (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
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bioRxiv - Cell Biology 2021Quote: ... beads were pelleted and resuspended in 100 μl of phosphatase buffer with lambda protein phosphatase (P0753; New England Biolabs) before agitation at 30°C for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... centrifuged at 58,000 × g for 50 minutes at 4°C and the protein was batch purified using chitin beads (NEB). Protein-bound chitin beads were washed with lysis buffer and high salt buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: All recombinant CPK28 clones were transformed into lambda phosphatase-expressing BL21 (DE3) E.coli cells for the production of dephosphorylated proteins or into T7 Express cells (New England Biolabs) for production of hyperphosphorylated protein (53) ...
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bioRxiv - Microbiology 2021Quote: ... benthamiana using the method described in the immunoblotting section were treated with Protein Deglycosylation Mix II (New England BioLabs) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... One sample was left untreated and the other two were supplemented with 1X Protein MetalloPhosphatase buffer (New England Biolabs) and 1 mM MnCl2 only or together with 1 μl Lambda Protein Phosphatase (New England Biolabs) ...
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bioRxiv - Biophysics 2021Quote: ... All proteins were purified and labeled with BG-surface Alexa 647 and BC-surface Dy547 dyes (New England Biolabs) as previously described (Murayama and Uhlmann ...
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bioRxiv - Cell Biology 2020Quote: The remainder of the protein was incubated with 50 μl of Ni-NTA Magnetic Beads (New England Biolabs, Inc.) on a rocker for 45 min at room temperature ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... 1:8 dilution Cas9 protein (New England Biolabs, cat. M0386M; diluted in buffer B, New England Biolabs, cat. B802S) and 1:40 dilution of phenol red (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and protein was purified on amylose resin (NEB) including a high salt wash with buffer containing 25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Protein samples were boiled in 1X Denaturing Buffer and incubated with PNGase F or Endo Hf (New England Biolabs) for 1-1.5 hr at 37°C following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were incubated with 0.9 mM MnCl2 and 7 U/μl lysate lambda protein phosphatase (λPP; New England BioLabs), or 0.9 mM MnCl2 and H2O as control ...
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bioRxiv - Neuroscience 2020Quote: ... To perform in vitro phosphorylation 5 μg of purified GST-NL2CT (WT or S714D) fusion protein was incubated with 2.5kUnits purified catalytic subunit of PKA (NEB) supplemented with 200 μM ATP at 30°C for the indicated time points.
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bioRxiv - Biochemistry 2022Quote: ... The N-linked glycans and the CBP tag were cleaved by endoglycosidase H (Endo H, ∼0.2 mg per 10–15 mg purified protein, New England Biolabs) and HRV-3C protease (∼2 mg per 10–15 mg purified proteins ...