Labshake search
Citations for New England Biolabs :
801 - 850 of 2294 citations for Mouse PNP shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2024Quote: ... Plasmids were digested with restriction enzymes from New England Biolabs (NEB, Ipswich, MA). Ligations used T4 DNA ligase (NEB M0202 ...
-
bioRxiv - Systems Biology 2024Quote: ... 2-10 µg plasmid DNA was digested with BsaI-HF-v2 (NEB R3733) in a 100 µl reaction for 4 h at 37°C and then spin-purified.
-
bioRxiv - Molecular Biology 2024Quote: Plasmid DNA was purified from Escherichia coli DH5α competent cells (New England Biolabs) using a Qiagen Miniprep kit ...
-
bioRxiv - Bioengineering 2023Quote: ... The plasmids were ligated based on Gibson assembly (New England BioLabs master mix) of plasmid backbone and insert sequences ...
-
bioRxiv - Cell Biology 2024Quote: ... the plasmid was also treated with 1.5 µl of quick-CIP (NEB, M0525). Following digestion ...
-
bioRxiv - Cell Biology 2024Quote: ... These plasmids were transformed into E.coli BL21 DE3 competent cell (New England Biolabs) and plated on agar plates containing Ampicillin or Kanamycin ...
-
bioRxiv - Microbiology 2024Quote: ... These plasmids (Table S6) in combination with the cell-free system PURExpress (NEB) were used to produce bacteriocins in vitro ...
-
bioRxiv - Molecular Biology 2024Quote: ... 60 μg of plasmid DNA was linearized with AhdI (New England Biolabs, UK) and extracted with phenol-chloroform for injection.
-
bioRxiv - Molecular Biology 2024Quote: ... gRNA-Cas9 plasmids were generated upon cleavage with BbsI restriction enzyme (NEB, R3539M) of the px330 plasmid following NEB instruction ...
-
bioRxiv - Microbiology 2023Quote: ... the F1 to F7 plasmids were digested with the PaqCI enzyme (R0745L, NEB) and assembled in vitro using T4 ligase (M0202M ...
-
bioRxiv - Microbiology 2024Quote: ... Plasmids and PCRs were digested with the appropriate restriction enzymes (New England Biolabs), purified ...
-
bioRxiv - Cell Biology 2019Quote: ... mouse anti-MBP (New England Biolabs, RRID:AB_ 1559738), used at 1:5000 ...
-
bioRxiv - Cancer Biology 2022Quote: ... mouse anti-PPARα (1:25, Arigo Biolabs ARG55240). Alexa Fluor conjugated secondary antibodies (ThermoFisher Scientific ...
-
bioRxiv - Biophysics 2021Quote: ... The plasmid was digested with XhoI and SacI (New England BioLabs, Ipswich, MA, USA) to remove the mutant Gsα sequence and purified via electrophoresis and gel extraction kit (Bio Basic ...
-
bioRxiv - Bioengineering 2022Quote: ... Sequence verified plasmids were transformed into BL21(DE3) Escherichia coli cells (New England Biolabs) and plated on LB medium supplemented with 100 μg mL−1 ampicillin ...
-
bioRxiv - Molecular Biology 2021Quote: ... The digested plasmid and amplified DNA library were assembled using Gibson Assembly reaction (NEB) using the insert ...
-
bioRxiv - Microbiology 2019Quote: ... Insert DNA and plasmid pBA169 were digested with EcoRI and BamHI (New England Biolabs) and ligated overnight before transformation into chemically competent E ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... The proA** expression plasmid was constructed using the Q5 Site-Directed Mutagenesis Kit (NEB).
-
bioRxiv - Biophysics 2021Quote: MukF Flag-tagged fragments were expressed from pET 21 plasmids in C3013I cells (NEB).The Flag-tagged MukE and MuF were at the C-terminus ...
-
bioRxiv - Microbiology 2021Quote: ... Correct plasmid sequence in isolated clonal bacteria was confirmed by both AscI/AatII (NEB) restriction digest and Sanger sequencing (University of Utah DNA Sequencing Core) ...
-
bioRxiv - Biochemistry 2020Quote: ... Plasmids were constructed by ligating PCR products amplified with Q5 polymerase (New England Biolabs) using Golden Gate assembly (87) ...
-
bioRxiv - Microbiology 2021Quote: ... we performed test-digestion of the generated plasmids with restriction enzymes (New England Biolabs), and analyzed the fragment pattern via gel electrophoresis ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... All plasmids were cloned into high-efficiency chemically competent DH5α (NEB, Ipswich, MA, USA). Plasmids were purified using PureYield miniprep kits (Promega ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The entire plasmid was PCR amplified using Q5 DNA Polymerase (New England BioLabs Inc.) with the following degenerate primers where N indicates any base ...
-
bioRxiv - Microbiology 2021Quote: ... These plasmids were generated using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554) instructions based on the previously described codon-optimized pD10-3xFlag according to the manufacturer’s [35] ...
-
bioRxiv - Microbiology 2021Quote: ... Virus stocks were recovered following linearization of infectious clone plasmids using NotI-HF (NEB), in vitro transcription ...
-
bioRxiv - Genomics 2021Quote: ... ENGRAM plasmid and PCR product from above were digested with BsmBI (NEB, buffer 3.1) at 55°C for 1h and purified for ligation ...
-
bioRxiv - Microbiology 2021Quote: ... acnes lysates were incubated with 0.5 ug plasmid DNA together with different buffers (NEB) supplemented with or without ATP or SAM ...
-
bioRxiv - Microbiology 2021Quote: ... the plasmid pMTL83152::Fncas12a was first digested using BbvCI and CIP (New England Biolabs) for 3h at 37°C ...
-
bioRxiv - Synthetic Biology 2021Quote: ... gBlocks fragment and backbone plasmid was ligated by T4 DNA ligase (NEB cat# M0202S) at 16 °C overnight ...
-
bioRxiv - Microbiology 2021Quote: ... were isolated using the Monarch® Plasmid Miniprep Kit (New England BioLabs Inc., USA), according to manufacturer’s instructions ...
-
bioRxiv - Biophysics 2020Quote: ... The TPCR-amplified plasmid DNA was treated with DpnI (New England Biolabs, Ipswich, MA) to remove any parental plasmid used as a template to construct the library ...
-
bioRxiv - Genomics 2022Quote: ... 8ug of STARR-seq plasmid was digested with 20uL AgeI-HF® (R3552, NEB), 20uL SalI-HF® (R3138 ...
-
bioRxiv - Biochemistry 2022Quote: ... All other plasmids were cloned by Gibson Assembly using NEBuilder HiFi (New England Biolabs). Mutations were introduced using primers containing the desired mutations ...
-
bioRxiv - Molecular Biology 2022Quote: ... Plasmid cloning and multiplication were performed in Escherichia coli DH5α (New England Biolabs, USA). E ...
-
bioRxiv - Microbiology 2022Quote: ... All DNA amplicons for plasmids constructs were amplified with high fidelity Phusion Polymerase (NEB) to minimize replication errors ...
-
bioRxiv - Molecular Biology 2022Quote: ... cloned into the pCRISPRia-v2 plasmid digested with BstXI and BlpI (New England Biolabs). Following the sgRNA transduction and puromycin selection ...
-
bioRxiv - Cell Biology 2022Quote: ... plasmid clones containing the correct insert were linearized with DraI (#R0129S, New England Biolabs) and sgRNA was synthesized in vitro using a Maxiscript T7 Transcription Kit (#AM1312 ...
-
bioRxiv - Molecular Biology 2020Quote: All plasmids were cloned using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) and mutations were made using the Q5 Site-directed mutagenesis kit (New England Biolabs) ...
-
bioRxiv - Microbiology 2021Quote: ... Digested plasmid was then de-phosphorylated with Shrimp Alkaline Phosphatase (rSAP) (New England Biolabs) for 30 min at 37°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... Most of the plasmids were assembled using the NEBuilder HiFi DNA assembly mastermix (NEB) following manufacturer’s recommendations ...
-
bioRxiv - Plant Biology 2021Quote: ... The inserts were cloned into pLRE::GFP plasmid linearized with BamHI (NEB, Catalog # R0136) and SalI (NEB ...
-
bioRxiv - Cancer Biology 2021Quote: ... Single gRNAs were cloned into LentiCRISPR 2.1 plasmid [57] by BsmBI (New England BioLabs) digestion and Gibson Assembly (New England BioLabs) ...
-
bioRxiv - Neuroscience 2020Quote: ... The ensuing plasmid was then mutated using the New England Biolabs (NEB, Ipswich, MA) mutagenesis Q5 kit in 29 different reactions ...
-
bioRxiv - Biochemistry 2019Quote: ... Plasmids were amplified by transformation into chemically competent Escherichia coli DH5α (New England Biolabs), which was plated onto LB agar (10 g/L tryptone ...
-
bioRxiv - Biophysics 2019Quote: ... we performed a double digest of TH1007 and the pSNAPf plasmid (New England Biolabs) with AgeI and BamHI ...
-
bioRxiv - Molecular Biology 2020Quote: ... and inserted into the opened plasmid with NEBuilder reagents (New England Biolabs; Berverley, MA) to create plasmid pBbRad51NLHAglmS (pBRNLHg ...
-
bioRxiv - Biochemistry 2019Quote: ... Nanobody and opto-nanobody plasmids were transformed into Shuffle T7 Express E.coli cells (NEB) and eGFP and mCherry plasmids were transformed into One Shot Top 10 cells (Invitrogen) ...
-
bioRxiv - Developmental Biology 2020Quote: ... and cloned into an SEC backbone plasmid (NEBuilder HiFi DNA Assembly Master Mix, NEB). The modified Cas9/sgRNA plasmid ...
-
bioRxiv - Molecular Biology 2020Quote: ... This U6-2xBsmBI-Tracr-HygroR plasmid was digested with the BsmBI restriction enzymes (NEB), after which the CRISPR inserts were ligated in using T4 DNA ligase (NEB) ...