Labshake search
Citations for New England Biolabs :
801 - 850 of 10000+ citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... RNA-seq libraries were generated using the NEBNext Globin&rRNA depletion kit and the NEBNext UltraII Directional RNA Library prep kit according to the manufacturer’s instructions (New England Biolabs, MA, USA). Sequencing experiments were performed using NextSeq 500 (Illumina ...
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bioRxiv - Genomics 2022Quote: We constructed NEBNext libraries with 200- and 10-ng RNA by following the guideline of NEBNext Ultra II RNA Library Prep Kit for Illumina kit (NEB, E7770). SmartSeq2 libraries with single cells were prepared following the protocol that was reported by Picelli ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized with RevertAid First Strand cDNA Synthesis Kit (ThermoScientific) reverse transcription kit and PCR was carried out using OneTaq® DNA Polymerase reagents (NEB) using conditions previously detailed and oligonucleotides described in Suppl ...
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bioRxiv - Cell Biology 2024Quote: ... samples were run on a 1% agarose gel and areas corresponding to the expected size were excised and purified from the gel using a gel extraction kit (Monarch DNA Gel Extraction Kit, NEB, USA). This PCR product was used for a second round of amplification followed by gel purification ...
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bioRxiv - Biophysics 2023Quote: ... Vector DNA was linearized with NheI or MluI and cRNA synthesized in vitro using the SP6 or T7 AmpliCap Max High Yield Message Maker Kit (Cellscript, USA) or HiScribe® T7 ARCA mRNA Kit (New England Biolabs) and stored at −20°C (for frequent use ...
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bioRxiv - Molecular Biology 2022Quote: ... Library quality was checked by Agilent BioAnalyzer 2100 using the High Sensitivity DNA kit (Part number 5067-4626) and libraries were quantified using the Library Quant Kit for Illumina (NEB #7630). Libraries were then sequenced using a NextSeq500 platform (75-base-pair (bp ...
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bioRxiv - Molecular Biology 2022Quote: ... Library quality was checked by Agilent BioAnalyzer 2100 using the DNA 1000 kit (Part number 5067-1504) and libraries were quantified using the Library Quant Kit for Illumina (NEB #7630). Libraries were then sequenced using a NextSeq500 platform (75-base-pair (bp ...
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bioRxiv - Biochemistry 2023Quote: ... loci were amplified using the NGSgo®-MX6-1 kit (GenDx) and multiplexed sequencing libraries were prepared using the Ultra™ II FS DNA Library Prep Kit for Illumina (NEB). Libraries were sequenced on a NovaSeq 6000 system (Illumina ...
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bioRxiv - Immunology 2023Quote: ... These amplicons were used as templates for in vitro transcription using the HiScribe T7 mRNA Kit with CleanCap® Reagent AG kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription into cDNA was performed using a cDNA synthesis kit (ProtoScript First Strand cDNA Synthesis Kit, New England BioLabs, Ipswich) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were generated using the NEBNext Globin&rRNA depletion kit and the NEBNext UltraII Directional RNA Library prep kit according to the manufacturer’s protocols (New England Biolabs, MA, USA), as described previously58 ...
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bioRxiv - Biophysics 2024Quote: ... Vector DNA was linearized with NheI or MluI and mRNA synthesized in vitro using the SP6 or T7 AmpliCap Max High Yield Message Maker Kit (Cellscript, USA) or HiScribe® T7 ARCA mRNA Kit (New England Biolabs).
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bioRxiv - Evolutionary Biology 2024Quote: ... Poly-A selection for mRNA enrichment was performed using the corresponding NEB kit (#E7490) followed by library preparation using the directional RNA library prep kit from NEB (#E7765). An additional NEB kit Low Input RNA library prep kit (#E6420 ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were qualified on Agilent 2100 Bioanalyzer using High Sensitivity DNA Kit and quantified by qPCR using NEBNext Library Quant Kit for Illumina (NEB, E7630). Equal molarity of each library was pooled and subjected to sequencing on Illumina NovaSeq 6000 platform at the McGill University and Génome Québec Innovation Centre to generate 100 bp paired-end reads.
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bioRxiv - Biophysics 2024Quote: ... Vector DNA was linearized with NheI or MluI and cRNA synthesized in vitro using the SP6 or T7 AmpliCap Max High Yield Message Maker Kit (Cellscript, USA) or HiScribe® T7 ARCA mRNA Kit (New England Biolabs) and stored at -20°C ...
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bioRxiv - Developmental Biology 2020Quote: A Fragment Analyzer™ Automated CE System (Analysis Kit: cat.: DNF-474-0500) was used to assess the fragment distributions and a Library Quant Kit for Illumina (NEB, cat.: E7630L) was used to measure molar concentrations ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μg/ml glycogen) and DNA purification was carried out with the PCR cleanup kit (NEB, Monarch PCR & DNA Cleanup Kit, T1030). About 10ng of the purified CUT&RUN DNA was used for preparation of multiplexed libraries with the NEB Ultra II DNA Library Prep Kit per manufacturer’s instruction (NEB #E7103) ...
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bioRxiv - Plant Biology 2021Quote: ... RNA-seq libraries were synthesized using an NEB Next Ultra RNA Library Prep Kit for Illumina and an NEB Next Adaptor for Illumina Kit (New England Biolabs, MA, USA). Paired-end sequencing was performed using the HiSeqXten (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA sequencing libraries were prepared from 100 ng of input RNA using the non-directional kit NEBNext Ultra™ II RNA Library Prep Kit for Illumina (NEB, #E7775) with the NEBNext Poly(A ...
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bioRxiv - Microbiology 2022Quote: Site directed mutagenesis was carried out using Q5® Site-Directed Mutagenesis Kit using manufacturer’s protocol (source of kit, NEB or else) using primers carrying the altered codon were designed using the online tool OligoCalc ...
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bioRxiv - Immunology 2023Quote: The total RNA of macrophage groups was isolated with a Nucleo-Spin RNA kit (Macharey Nagel, Germany) or Monarch Total RNA Miniprep Kit (New England Biolabs, Massachusetts, ABD). The purity and quantity of RNA were evaluated by a Nanodrop spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated using a genomic DNA extraction kit (Monarch Genomic DNA Purification Kit, New England Biolabs, Frankfurt am Main, Germany) and the PCR was optimized to yield a single amplicon ...
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bioRxiv - Microbiology 2023Quote: ... Stranded RNA-seq libraries were constructed using NEBNext poly(A) mRNA isolation kit along with the SWIFT RNA Library Kit (NEB, MA, USA) according to the established protocols at Laboratory for Molecular Biology and Cytometry Research (LMBCR ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA was purified using the QIAquick PCR purification kit and it was prepared for Illumina sequencing using the NEBNext Ultra II A Library Prep Kit (New England Biolabs; Cat# E7645L).
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bioRxiv - Systems Biology 2024Quote: ... RNAs were extracted using Qiagen RNeasyPlus Mini kit and RNA library preparation performed using NEBNext Ultra II DirecMonal RNA Library prep Kit (NEB, Lynn, MA) following the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2020Quote: ... respectively by Phusion® High-Fidelity PCR Kit (NEB) (Harashima & Schnittger ...
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bioRxiv - Cancer Biology 2021Quote: ... After purified with Monarch RNA Cleanup Kit (NEB Biolabs), RNAs were polyadenylated with E ...
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bioRxiv - Cancer Biology 2021Quote: ... After purified with Monarch RNA Cleanup Kit (NEB Biolabs), RNAs were polyadenylated with E ...
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bioRxiv - Molecular Biology 2020Quote: ... and qPCR analysis by NEB Next Library Quant Kit for Illumina (New England Biolabs). All sequencing was performed using an Illumina MiSeq (Illumina ...
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bioRxiv - Cell Biology 2020Quote: Libraries were prepared using Next Ultra II kits (NEB) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Using the NEBuilder HiFi DNA assembly cloning kit (NEB), EBOV VP30 was inserted into a pLenti6.3 vector (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... and reverse transcribed using Lunascript kit (New England Biolabs). qPCR was performed on a Light Cycler 480 (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... purified with the Monarch PCR & DNA Cleanup Kit (NEB) and ligated with T4 DNA ligase (NEB) ...
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bioRxiv - Developmental Biology 2021Quote: ... Using Q5® Site-Directed Mutagenesis Kit (NEB, E0554), we generated mutations in each of the two most highly conserved Rbpj binding sites (m1 and m2 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... NEBNext® Microbiome DNA Enrichment Kit (New England BioLabs) was used for increasing the proportion of bacterial DNA (via the procedure of selective binding and disposing of methylated host DNA) ...
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bioRxiv - Developmental Biology 2021Quote: ... we used the Q5 Site Directed Mutagenesis kit (NEB) to generate the sqhT20A mutation in the pattB -sqhWT construct using oligos H334 and H335 (See Table 2) ...
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bioRxiv - Genomics 2020Quote: ... We used the NEBuilder HiFi DNA Assembly Kit (NEB) to assemble the purified inserts and the backbone of MPRA plasmid (previously digested with EcoRV) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and the Unique Dual Indexing Kit (New England Biolabs), with reagent volumes reduced to one-fifteenth of recommended volumes ...
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bioRxiv - Molecular Biology 2021Quote: ... with the NEBNext rRNA Depletion Kit (NEB, cat# E6310L) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... NEBuilder® HiFi DNA Assembly Cloning Kit (NEB, #E5520) was used according to user’s manual ...
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bioRxiv - Genomics 2020Quote: ... and NEB Quick DNA ligase kit (NEB cat. E6056S) by incubation at 16°C overnight without a heated lid ...
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bioRxiv - Molecular Biology 2021Quote: ... and ProtoScript II First Strand cDNA Synthesis Kit (NEB) with Oligo-dT primers ...
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bioRxiv - Genomics 2021Quote: ... NEBNext multiplex oligos for Illumina Kit (New England Biolabs) was used in adapter ligation and PCR amplification steps ...
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bioRxiv - Genomics 2020Quote: ... using the NEBNext Ultra DNA Library kit (NEB #E7370) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... ProtoScript-II first-strand cDNA synthesis kit (NEB, USA), Brilliant-III ultrafast SYBR Green qPCR master mix (Agilent Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... or a PURExpress bacterial IVT kit (New England Biolabs), following the manufacturer’s recommended protocol similar to that described previously45 ...
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bioRxiv - Microbiology 2021Quote: ... The WarmStart LAMP Kit (Cat# E1700S, New England BioLabs) was utilized using SARS-CoV-2 specific primers[43] or SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (New England Biolabs) ...
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bioRxiv - Bioengineering 2021Quote: ... using NEBuilder HiFi DNA Assembly Cloning Kit from NEB. Synthetic DNA was purchased from IDT as gBlocks (Integrated DNA Technologies ...
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bioRxiv - Biochemistry 2021Quote: A Q5 site-directed mutagenesis kit (NEB, Ipswitch, MA) was used to generate pcDNA3.1-p300-FLAG from the plasmid pcDNA3.1-p300-His6 obtained from Addgene (Catalog number 23252 ...
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bioRxiv - Biophysics 2021Quote: ... and generated using NEBNext DNA Library Prep Kit (NEB). Samples were indexed with unique barcodes using NEBNext Multiplex Oligos for Illumina (NEB) ...