Labshake search
Citations for New England Biolabs :
801 - 850 of 2481 citations for Dengue Virus Serotype 2 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2022Quote: ... MBP-fusion proteins were purified using Amylose Resin (New England Biolabs, E8021), following the manufacturer’s instruction with minor modification ...
-
bioRxiv - Microbiology 2023Quote: ... and then associated with EnGen Spy Cas9 NLS protein (New England BioLabs) using the 4D-Nucleofector system (Lonza) ...
-
bioRxiv - Cell Biology 2023Quote: ... and MBP-tagged proteins were purified on Amylose resin (New England Biolabs).
-
bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins were purified on glutathione-Sepharose beads (New England Biolabs), and MBP-tagged proteins were purified on Amylose resin (New England Biolabs).
-
bioRxiv - Microbiology 2023Quote: ... the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, E6800L) was used as described previously (31) ...
-
bioRxiv - Cell Biology 2023Quote: ... 25 µl protein A magnetic beads S1425S (New England Biolabs; Ipswich, MA) were washed 3 times in PBS and incubated overnight with 2-3 µg of antibody directed against CISD34 at 4°C with gentle mix ...
-
bioRxiv - Developmental Biology 2023Quote: ... sgRNA was incubated at room temperature with Cas9 protein (NEB, Cat# M0646T). AB* embryos were injected at the one-cell stage with the sgRNA and Cas9 cocktail in a 1nL volume at 25pg sgRNA/nL ...
-
bioRxiv - Cell Biology 2023Quote: ... Cell pellets were lysed and subsequently incubated with λ-protein phosphatase (NEB) for 1 h at 30°C according to the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The proteins were purified by NEBExpress Ni Spin Columns (New England Biolabs) following manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteins with MBP-tag were loaded onto amylose resin (New England Biolabs) after elution from Ni-NTA ...
-
bioRxiv - Neuroscience 2023Quote: ... This complex was co-injected with Cas9 protein (M0386M, NEB, Ipswich USA) into the yolk of 1-cell stage WT zebrafish embryos ...
-
bioRxiv - Microbiology 2023Quote: ... 5 μL of 2X protein synthesis buffer (New England Biolabs, Beverly, MA), 0.25 μL of RNaseOUT RNAse inhibitor (Thermo-Fisher ...
-
bioRxiv - Molecular Biology 2024Quote: ... including a Blue Pre-stained Broad Range Protein Standard (New England Biolabs). Gels were either stained (with 0.5% (w/v ...
-
bioRxiv - Microbiology 2024Quote: ... The molecular weight standards used were unstained broad range protein standards (NEB) and then the gel was run at 100 volts until the dye front was 1 cm from the bottom of the gel and then stained one hour in Coomassie blue stain (50% methanol ...
-
bioRxiv - Microbiology 2020Quote: ... or 2 μL of Shortcut RNase III (0.02 L/μL; NEB) were added and incubated for 45 seconds ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2 µl of 10x NEBuffer 3 (cat#B7003, New England Biolabs), and 13 µl of water were incubated in a thermocycler (cat#EP950040025 ...
-
bioRxiv - Genomics 2020Quote: ... with 2 μl (5 U/μl) of Klenow fragment exo- (NEB) in a final volume of 55 μl by incubation at 37°C for 30 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were resuspended in 2% bovine serum albumin (New England BioLabs) and were transferred immediately in ice to the genomics core laboratory.
-
bioRxiv - Molecular Biology 2021Quote: ... 2 µg BSA dealt as negative control (NEB, Ipswich, MA, USA). Samples were applied in a final volume of 100 µl coating buffer (100 mM Tris-HCL pH 8 ...
-
bioRxiv - Bioengineering 2022Quote: ... 1 μL of 2-Log DNA Ladder (200 μg/mL; NEB) is mixed with 1 μL of Gel Loading Dye (6x ...
-
bioRxiv - Genomics 2019Quote: ... 10 µL T4 RNA ligase 2 truncated (200 U/µL, NEB) was added ...
-
bioRxiv - Systems Biology 2020Quote: ... and 2 μL NEBNext Second Strand Synthesis Enzyme Mix (E6111S, NEB) were added to the purified cDNA ...
-
bioRxiv - Cell Biology 2019Quote: ... 2 µL of T4 RNA ligase II truncated KQ (NEB M0373), 0.5 µL of SUPERaseIN ...
-
bioRxiv - Cell Biology 2021Quote: ... and digested with 2 U/μl of DpnII restriction nuclease (NEB) at 37 °C overnight ...
-
bioRxiv - Immunology 2021Quote: ... a mix containing 2 μl of RNAse H (NEB, Cat. #M0297S), 1 μl of E ...
-
bioRxiv - Genomics 2021Quote: ... the reaction was incubated with 2 μL of USER (NEB #5508) in 1X CutSmart Buffer at 37 °C for 15 min before final purification with Zymo Oligo Clean & Concentrator kit ...
-
bioRxiv - Synthetic Biology 2021Quote: ... SARS-CoV-2 RNA was replaced with nuclease free water (NEB) for the assay without the target RNA ...
-
bioRxiv - Microbiology 2020Quote: ... 12.5 μl Q5 high-fidelity 2*master mix (New England Biolabs), 8.5 μl dH2O ...
-
bioRxiv - Genomics 2021Quote: ... 2 μL of 1 mg/ml bovine serum albumin solution (NEB), 1 μL of hOGG1 (ProSpec TechnoGene Ltd. ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 10 units/µL T4 RNA Ligase 2 (truncated K227Q, NEB). The ligation reaction was incubated for 3 hours at 22 °C ...
-
bioRxiv - Microbiology 2020Quote: ... after which 2 µL calf intestinal phosphatase (10,000 U/mL, NEB) was added to the digest and incubated for 1h at 37°C ...
-
bioRxiv - Cancer Biology 2020Quote: ... and 4 μl T4 RNA ligase 2 (NEB, catalog no. M0239) for 16 hours at 16°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... 800U of truncated T4 RNA ligase 2 K227Q (New England Biolabs) in supplied PNK buffer with RNAsin (Promega ...
-
bioRxiv - Biochemistry 2022Quote: ... and RNA ligase 2 of bacteriophage T4 (New England BioLabs, Inc.). Reactions were incubated for one hour at 25 °C and ligated duplexes were purified by 1ml monoQ column (Cytiva ...
-
bioRxiv - Microbiology 2022Quote: ... An additional 2 μl RNase A (20 mg/ml, NEB #T3018) was added and followed by 5 min incubation at 56 °C to remove residual RNA in the sample ...
-
bioRxiv - Plant Biology 2021Quote: ... in 1X NEB Buffer 2 (New England BioLabs; Ipswitch, MA, USA), and 0.1 mM dATP for 30 minutes at 37°C ...
-
bioRxiv - Microbiology 2020Quote: ... 1–2 mg of RNA was treated with DNAse I (NEB), x µL and 2 µL of this reaction was used to make cDNA ...
-
bioRxiv - Molecular Biology 2020Quote: ... After the addition of 50ul of 10X NEBBuffer 2 (NEB, B7002) and 1ul of MboI restriction enzyme (NEB ...
-
bioRxiv - Genomics 2020Quote: ... 2 μL 5x Q5 reaction buffer (New England Biolabs, Ipswich, MA), 0.2 μL 10 mM dNTPs (Kapa Biosystems ...
-
bioRxiv - Developmental Biology 2022Quote: ... 2 µl NEB-Next High-Fidelity PCR Mix (NEB, cat # M0541S), 5 µl of SYBR Green (Invitrogen™ ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... 2 μl of the rare-cutter AseI (New England Biolabs, UK) was added and incubated at 30 °C overnight ...
-
bioRxiv - Biophysics 2019Quote: ... The annealed strands were ligated with T4 RNA ligase 2 (NEB), in 20 μl reactions for 1h at 37°C ...
-
bioRxiv - Biochemistry 2019Quote: ... 2 μl of 10X T4 RNA Ligase Buffer (New England Biolabs), 1 μl of 2 μM 5’-adenylated RNA linker ...
-
bioRxiv - Genomics 2021Quote: ... 50 μL NEB Buffer 2 and 375 U MboI (NEB R0147M) were added and allowed to incubate for 2 hours at 37°C rotating ...
-
bioRxiv - Biochemistry 2021Quote: ... 400 μM ATP (1x T4 RNA ligase 2 reaction buffer (NEB)) including 0.25 units/μL T4 RNA ligase 2 (Neb ...
-
bioRxiv - Microbiology 2021Quote: ... Ligation was conducted using T4 double stranded RNA ligase 2 (NEB) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Oligos (2 μM each) were annealed in 1X CutSmart buffer (NEB) at 95°C for 5 min followed by cooling to room temperature ...
-
bioRxiv - Molecular Biology 2020Quote: ... 3 mM MgCl2 and 2 μL (10 u) RNase H (NEB) in order to digest poly(A ...
-
bioRxiv - Microbiology 2022Quote: ... 2 µg BSA and 3 units of T4 DNA polymerase (NEB) and incubated at 20 °C for 30 minutes ...
-
bioRxiv - Genomics 2022Quote: ... and 2 μl of Klenow Fragment (3’→5’ exo-) (M0212L, NEB). The reactions were incubated at 37ºC for 30 minutes and then cleaned up with a Clean and Concentrate-25 column (Zymo) ...