Labshake search
Citations for New England Biolabs :
8401 - 8450 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: cDNA synthesis on all RNA samples was carried out using LunaScript RT SuperMix Kit (NEB) with 16 μl template RNA in 20 μl reactions according to the manufacturer’s instructions.
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bioRxiv - Genetics 2023Quote: ... and were quantified with the NEBNext Library Quant Kit for Illumina (New England BioLabs E7630L). Libraries were pooled to a final concentration of 4 nM and submitted to the Johns Hopkins Genomics Research Core Facility for high-throughput sequencing on the Illumina NovaSeq 6000 platform ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were generated using the NEBNext Ultra RNA library prep kit for Illumina (NEB), and sequencing was performed on an Illumina NovaSeq 6000 platform ...
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bioRxiv - Microbiology 2023Quote: ... the Monarch® HMW DNA Extraction kit for Tissue (New England Biolabs, Ipswich, MA, USA) was used to improve DNA fragment length ...
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bioRxiv - Molecular Biology 2023Quote: HLTF’s cDNA on the pcDNA3.1(+) backbone54 was mutated using Q5 Site-Directed Mutagenesis kit (NEB) to generate the C760S and R890Q mutant ...
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bioRxiv - Molecular Biology 2023Quote: ... using the High Sensitivity DNA Assay and NEBNext Library Quant Kit for Illumina (NEB, E7630). Finally ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was done with 100ng of RNA with the Luna RT-qPCR kit (NEB) using primers targeting beta actin as a control (Table S2) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was then prepared for sequencing with the NEBNext Small RNA library Prep kit (NEB). Sequencing libraries were visualized using an Agilent 2200 Tapestation and sequencing was done using the Novaseq 6000 SP300 cartridge (Illumina).
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bioRxiv - Microbiology 2023Quote: ... Nef deletion Reverse: 5’-AGATCTACAGCTGCCTTGTAAGTCATTGG-3’) using Q5® Site-Directed Mutagenesis Kit (NEB #E0554S).
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bioRxiv - Bioengineering 2023Quote: ... The mSA S27A mutation was created by site-directed mutagenesis using commercially-available kits (NEB). 4NBX.B_C103 and 2X89.A_CCAA_C57 sequences were directly ordered from IDT ...
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bioRxiv - Microbiology 2023Quote: ... RNAs were transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs). Reactions were set up as per manufacturer’s instructions with the addition of 4 units of Murine RNase Inhibitor (NEB ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using a NEBNext Ultra II DNA library prep kit (NEB, Cat# E7645S) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Cell Biology 2023Quote: The libraries were constructed using NEBNext Ultra RNA Library Prep Kit for Illumina (E7530L, NEB). Sequencing was performed on an Illumina NovaSeq 6000 platform with 2D×D150 bp pair-end reads at Novogene ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were performed with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to the published protocol50 ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were prepared using NEBNext® Ultra™ Directional RNA Library Prep Kit (NEB, E7420) and amplified by PCR with KAPA HiFi HotStart Ready Mix (Roche) ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs #T2010S). Reverse transcription was performed using iScript Reverse Transcription Supermix (BioRad #1708841 ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA was extracted using Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB, T3050), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Amplification products were gel extracted using the Monarch® DNA Gel Extraction Kit (NEB T1020).
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bioRxiv - Cell Biology 2024Quote: ... First strand cDNA was synthesized using a Lunascript® RT SuperMix Kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... following the manufacturer’s guidelines provided with the library kit (E7560S, New England BioLabs Inc., USA). Monarch PCR purification kit (Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... Fragmented DNA samples was purified using the Monarch DNA Clean up Kit (New England Biolabs). The libraries were prepared from 5-10ng purified CUT&RUN-enriched DNA ...
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bioRxiv - Biochemistry 2024Quote: ... The W75K substitution was introduced using Q5 site-directed mutagenesis kit (New England Biolabs, NEB). Substitutions L80E ...
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bioRxiv - Biochemistry 2024Quote: ... The W75K substitution was introduced using Q5 site-directed mutagenesis kit (New England Biolabs, NEB). Substitutions L80E ...
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bioRxiv - Biochemistry 2024Quote: ... Libraries were then prepared on beads using NEBNext Ultra II DNA Library Prep kit (NEB) following manufacturers recommendations with 0.6 μM of KAPA Unique Dual-Indexed adapters (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was further processed using the Monarch RNA cleanup Kit (New England Biolabs, Ipswich, MA). Protocol steps were executed according to manufacturer’s instructions with slight modifications and the RNA was finally eluted in a total volume of 40ul of RNase/DNase free water ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA was depleted with the NEBNext rRNA depletion kit for bacteria (New England Biolabs) and a set of 20 pooled sequence-specific probes for Bc ...
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bioRxiv - Cell Biology 2024Quote: ... a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645S) was used according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (NEB; E7645) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: All protein mutagenesis was performed with the Q5 site-directed mutagenesis kit from NEB (E0554). Primers were designed with the NEBaseChanger tool (https://nebasechanger.neb.com/ ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using the Monarch Genomic DNA Purification Kit (New England Biolabs, Whitby, Canada) according to the manufacturer’s recommendations for DNA purification from gram-positive bacteria and Archaea ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA was depleted using the NEBNext rRNA Depletion Kit for bacteria (New England Biolabs), cDNA libraries were constructed using the NEB Ultra Directional RNA library prep kit for Illumina and sequenced on Illumina’s NextSeq 1000 platform at 2 × 100 bp read length ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was generated from 1 µg RNA using LunaScript RT SuperMix Kit (New England Biolabs). qPCR reactions were performed on an Applied Biosystems 7500 Real-Time PCR system using the Luna Universal qPCR MasterMix (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... The in vitro translation assay was performed using PURExpress In Vitro Protein Synthesis Kit (NEB). 24 μM of antibiotics were added individually to the translation mixture and incubated at 37 °C for 4 hours ...
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bioRxiv - Biochemistry 2024Quote: In Vitro Translation (IVT) was performed using the PURExpress in vitro protein synthesis kit (NEB). Each IVT reaction contained 4 μL of PURE Solution A ...
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bioRxiv - Biochemistry 2024Quote: ... The crude RNA was preliminarily purified using Monarch RNA Clean-up kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... quantified the two pools using an NEBNext Library Quant Kit for Illumina (New England Biolabs), and determined average fragment size and adaptor dimer removal on a Tapestation 4200 (Agilent ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted using the Monarch Total RNA Miniprep Kit (New England BioLabs #T2010S) and submitted to Azenta Life Sciences for library preparation and Illumina sequencing.
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bioRxiv - Developmental Biology 2024Quote: ... in combination with NEBNext Ultra II directional RNA library prep kit for Illumina (NEB #E7765) following manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: RT-qPCR was conducted using Luna®Universal One-Step RT-qPCR Kit (NEB E3005L) following the manufacturer’s documentation ...
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bioRxiv - Plant Biology 2024Quote: ... The concentration was estimated using the NEBNext Library Quant Kit for Illumina (New England Biolabs). Each 10 nM library pool (mim-tRNAseq or QuantM-tRNAseq ...
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bioRxiv - Synthetic Biology 2024Quote: ... Golden Gate Assembly Kit (BsaI-HF v2, E1601L) and the Gibson Assembly Protocol (NEB E5510) were employed ...
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bioRxiv - Synthetic Biology 2024Quote: RNA was extracted 48h after transfection using the Monarch total RNA miniprep kit (NEB T2010S) and stored at −80C until further processing ...
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bioRxiv - Synthetic Biology 2024Quote: mRNA was isolated from total RNA extracts using the magnetic mRNA Isolation Kit (NEB S1550S) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs, MA, USA) was performed on the RNA ...
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bioRxiv - Microbiology 2024Quote: ... Additional mutations/deletions were introduced in gp140-T/F07 using site directed mutagenesis kit (NEB). The sequences of the recombinant clones were verified through sequencing (Retrogen ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and purified the rest of the digestion product with Monarch Nucleic Acid Purification Kit (NEB). We quantified the amount of purified DNA using the Qubit dsDNA HS Assay (Thermofisher ...
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bioRxiv - Developmental Biology 2024Quote: ... mRNA was synthesized from linearized plasmid using HiScribe T7 high yield RNA synthesis kit (NEB), then capped with m7G (5’ ...
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bioRxiv - Cell Biology 2024Quote: ... The DNAse-digested RNA was purified using the Monarch RNA Cleanup kit (New England Biolabs) as recommended by the manufacturer and used as template for cDNA synthesis and SYBR Green-based qPCR (see quantitative real-time PCR (RT-qPCR ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries for sequencing were constructed with the NEB Next Ultra RNA Library Prep Kit (NEB) for Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... Y511A mutation in pBAD-His-BrxX was introduced using Q5 site-directed mutagenesis kit (NEB), while introduction of Y511A to the pBREX AL was carried by joining 2 plasmid fragments ...