Labshake search
Citations for New England Biolabs :
8251 - 8300 of 10000+ citations for 2 Arachidonoylglycerol 2 AG CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... 25 uL of LNB buffer from LSK-109 kit (Oxford Nanopore Technologies) and 10 uL of ligase (New England Biolabs) and and 5 uL of adapter from LSK-109 kit was then added the reaction mixture and mixed by gentle tapping ...
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bioRxiv - Cancer Biology 2020Quote: ... 30 ng total RNA (RQI≥8) was used for library preparation following the NEBNext Ultra RNA Library Prep Kit for Illumina protocol (New England BioLabs) with the Poly(A ...
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bioRxiv - Genomics 2020Quote: ... Directional RNA-seq libraries were prepared from 1000 ng total RNA per sample using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs), with initial polyA+ isolation ...
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bioRxiv - Genomics 2019Quote: Sequencing libraries were prepared as previously described (Belsky et al. 2015) with the following modifications: NEBNext multiplex oligos for Illumina kit (New England Biolabs) were used in adapter ligation and PCR steps ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we used it for metagenomic library preparation using NEBNext Ultra II DNA Library Prep Kit for Illumina (BioLabs, New England), with the target insert length of 350 bp ...
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bioRxiv - Immunology 2020Quote: RNA was extracted from 0.25×106 cells at day 12 of cell culture using the Monarch total RNA miniprep kit (New England Biolabs, T2010S) and quantified using the Qubit RNA HS Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The remaining steps of library preparation were done using the NEBNExt Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Sequencing was performed on an Illumina HiSeq 4000 with 100 bp paired-end reads.
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bioRxiv - Immunology 2020Quote: ... Resulting mRNA was prepared for RNA-seq using a NEB Ultra II RNA Sequencing Library Prep Kit (New England Biolabs) as per the manufacturer’s guidelines.
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bioRxiv - Microbiology 2021Quote: ... reverse: 5’-GATGGCGTGGAACCATGTC-3’) were obtained from the wild type plasmids pCMV-hnCoV-S via Q5 SiteDirected Mutagenesis Kit (NEB). pCMV-hnCoV-S-H501Y-Δ69/70 was obtained from pCMV-7.1-hnCoV-S-H501Y (forward ...
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bioRxiv - Molecular Biology 2021Quote: ... a 4946 bp fragment generated by PCR with primers Upf2-425-fwd and Upf2-425-rev containing a the entire UPF2 gene was inserted in to pRS425 cut with NotI and SalI using the NEBuilder High-Fidelity DNA Assembly Cloning Kit (New England Biolabs).
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bioRxiv - Microbiology 2020Quote: ... electrophoresed and the 200-bp bands were excised from agarose gel slice using Monarch DNA Gel extraction Kit (NEB, #T1020S). Then ...
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bioRxiv - Microbiology 2020Quote: ... 1-2 μg of total RNA from each sample (or complete sample if the yield was lower) was used for mRNA isolation (Magnetic mRNA Isolation Kit, NEB). First strand cDNA synthesis was performed using the SuperScript III First-Strand Synthesis System and a 1:1 mix of Oligo(dT ...
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bioRxiv - Microbiology 2020Quote: ... separate reactions were performed for the quantification of SARS-CoV-2 N and E gene transcripts as well as cellular RNaseP for normalization using the Luna Universal Probe One-Step RT-qPCR Kit (NEB) on a Bio-Rad CFX384 Touch system ...
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bioRxiv - Microbiology 2020Quote: BsaI digested fragment were gel purified by using Gel Extraction Kit (OMEGA) and ligated with T4 ligase (New England Biolabs) at room temperature for 1h ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were assembled from fragments (linearized vector, PCR products, and/or synthetic DNA) using the NEBuilder Hifi DNA assembly kit (New England Biolabs (NEB) E2621) ...
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bioRxiv - Microbiology 2020Quote: ... fragments were amplified by PCR with primers oJMP197 and oJMP198 from a 78 nt oligonucleotide followed by digestion with BsaI-HF-v2 and purification with the Monarch DNA purification kit (NEB) following the manufacturer’s oligonucleotide purification protocol ...
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bioRxiv - Microbiology 2020Quote: ... were created by replacing the entire env-coding sequence of F-MLV WT with amphotropic env-coding sequence from p-SV-A MLV env using NEBuilder HiFi DNA Assembly Kit (New England Biolabs). The primers used for swapping the env-coding regions were ...
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bioRxiv - Microbiology 2020Quote: ... subtilis 168 CA with the pJM14 integrative vector carrying the yxiD-yxxD flanking sites inserted using Gibson assembly kit from NEB. For inter-species competition L ...
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bioRxiv - Microbiology 2020Quote: ... Both mCherry and mEGFP were cloned with no linker after the Matrix domain in Gag via Gibson assembly with the HiFi DNA Assembly cloning kit (New England Biolabs). Similar constructs have been previously published ...
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bioRxiv - Plant Biology 2021Quote: ... The miR823-resistant CMT3 construct (rCMT3) was generated by PCR site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs) using the cCMT3 construct as a template to introduce six silent mutations as shown in Fig ...
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bioRxiv - Microbiology 2019Quote: ... cassette between SalI and BglII in the plasmid pGDB34 was isolated by digestion and gel purification and inserted between the same restriction sites in the plasmid pyPM2GT-EXP2-mNeonGreen14 with a T4 Quick Ligation kit (NEB), replacing the yDHODH cassette ...
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bioRxiv - Immunology 2021Quote: ... In vitro transcribed RNA was generated using the HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, Cat E2060S) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Illumina-compatible libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (catalog no. E7645S; New England BioLabs) and sequenced using an Illumina NovaSeq 6000 system in a paired-end 2x50- base pair (bp ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The extracted RNA was prepared for short-read sequencing library construction using the NEB NextUltra RNA-seq kit (New England Biolabs). The constructed libraries were sequenced with 150bp paired-end reads on an Illumina HiSeq 2500 and 6000 sequencing platforms ...
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bioRxiv - Immunology 2020Quote: ... 10ng or more of DNA was used to generate libraries according to the NEBNext RNA Library Prep Kit (New England Biolabs). Following the amplification step ...
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bioRxiv - Molecular Biology 2021Quote: ... gel-purified and cloned into SalI/SpeI-digested pAT006 (Tsuzuki et al., 2014) vector via HiFi DNAAssembly Cloning kit (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: ... The two fragments were cloned into AscI- digested pBYL2 vector (Mine et al., 2010) via HiFi DNA Assembly Cloning kit (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: ... Table S4) was cloned into XbaI/AscI-digested pBYL2 vector using the HiFi DNA Assembly Cloning kit (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: ... and then served as the DNA template for in vitro transcription reactions using the HiScribe T7 High Yield RNA Synthesis Kit (Cat. No. E2040S, NEB). Template DNA was removed by the addition of DNase I (Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... Paired-end libraries were prepared for four hog deer samples collected from Sunday Island in 2015 using a NebNext Ultra Library Prep Kit (BioLabs), with size selection of 500-600 base pairs (bp) ...
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bioRxiv - Molecular Biology 2020Quote: ... Wild-type (wt) gRNA was in vitro transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... mRNA was used to generate RNA-seq libraries using a NEBNext Ultra II RNA library prep kit for Illumina (New England Biolabs) following the manufacturer’s recommended protocol with the following parameters ...
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bioRxiv - Molecular Biology 2021Quote: ... Two biological replicates of RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) according the kit instructions ...
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bioRxiv - Immunology 2020Quote: ... RNA-seq libraries were constructed using the purified mRNA samples with NEBNext Ultra II Directional RNA library Prep Kit for Illumina (E7760S, NEB). The quality of these RNA-seq libraries were validated using 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Molecular Biology 2021Quote: ... EndoCβ-H1 and mouse islets was determined with an Agilent Bioanalyzer and mRNA enrichmed was performed from 500-1000ng of RNA using a NEBNext Poly(A) mRNA Magnetic Isolation Kit (NEB). Library preparation from mRNA and from miRISC-isolated RNA was performed using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Immunology 2021Quote: ... The enriched cDNA was converted to Illumina-compatible libraries using the NEBNext Ultra II DNA kit (New England Biolabs, Inc.) using 1ng input ...
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bioRxiv - Microbiology 2021Quote: ... and the DNA was repaired and A-tailed using the NEBNext DNA Library preparation kit for Illumina (New England Biolabs). After ligating Illumina compatible adapters to the DNA fragments ...
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bioRxiv - Microbiology 2021Quote: pTXB1 and pTYB21 derivatives for protein overproduction were constructed based on the guidelines provided with the IMPACT kit (New England Biolabs). The coding sequences of nepR1 ...
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bioRxiv - Immunology 2020Quote: ... and converted into RNA-seq libraries using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Mutations inducing amino acid substitutions in the vif region of infectious molecular clones or in the APOBEC3 expression constructs were introduced using Q5 site-directed mutagenesis kit (NEB). The presence of the desired mutations was confirmed by Sanger sequencing.
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bioRxiv - Immunology 2020Quote: ... We quantified RNA using a one-step RT qPCR reaction with the NEB Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs) and previously published TaqMan primers and probes (SARS-CoV-2 E_Sarbeco and hamster RPL18 ...
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bioRxiv - Microbiology 2021Quote: ... was used to make cDNA PCR templates from parasite RNA (Toxoplasma GT1; Plasmodium falciparum NF54) extracted with a Monarch Total RNA Miniprep Kit (New England Biolabs). Genomic DNA (Toxoplasma RH derivatives ...
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bioRxiv - Microbiology 2021Quote: ... A plasmid encoding an eGFP-containing EBOV antigenome was generated through assembly of fragments using the NEBuilder HiFi DNA assembly cloning kit (New England Biolabs (NEB)) ...
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bioRxiv - Molecular Biology 2021Quote: ... tropicalis pseudouridine synthase Pus7 was amplified from total RNA using the OneTaq One-step RT-PCR kit (New England Biolabs). XmaI and XhoI restriction sites were added with oligonucleotides and the resulting fragment was cloned into the p415Gal1 vector.
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bioRxiv - Immunology 2021Quote: ... and the RNA-seq libraries were generated with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Point mutations were introduced in the P and N sequences by site-directed mutagenesis using the Q5 site-directed mutagenesis Kit (New England Biolabs). Sequence analysis was carried out to check the integrity of all constructs ...
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bioRxiv - Microbiology 2020Quote: ... TEV was removed from the original plasmid and mEGFP was inserted in its place using a Gibson reaction via the HiFi DNA Assembly cloning kit (New England Biolabs).
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bioRxiv - Developmental Biology 2021Quote: ... each CBE4max-SpG and CBE4max-SpRY sequence has been inserted into pCS2+ plasmid linearized with EcoRI using the Gibson Assembly Cloning Kit (New England Biolabs). The fragment has been amplified using the primers F-5’-TGCAGGATCCCATCGATTCGGCCACCATGAAACGGACAG -3’ and R-5’-TAGAGGCTCGAGAGGCCTTGTCAGACTTTCCTCTTCTTCTTGG -3’ ...
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bioRxiv - Immunology 2021Quote: ... ii) fragmentation and adapter ligation (NEBNext® UltraTM II FS DNA Library Prep Kit for Illumina, NEB, cat. no. E7805S), and iii ...
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bioRxiv - Cell Biology 2021Quote: ... Stranded mRNA-Seq libraries were prepared from 250 ng of total RNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) implemented on the liquid handling robot Beckman i7 ...