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Citations for New England Biolabs :
8201 - 8250 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: BsaI digested fragment were gel purified by using Gel Extraction Kit (OMEGA) and ligated with T4 ligase (New England Biolabs) at room temperature for 1h ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were assembled from fragments (linearized vector, PCR products, and/or synthetic DNA) using the NEBuilder Hifi DNA assembly kit (New England Biolabs (NEB) E2621) ...
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bioRxiv - Microbiology 2020Quote: ... were created by replacing the entire env-coding sequence of F-MLV WT with amphotropic env-coding sequence from p-SV-A MLV env using NEBuilder HiFi DNA Assembly Kit (New England Biolabs). The primers used for swapping the env-coding regions were ...
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bioRxiv - Microbiology 2020Quote: ... subtilis 168 CA with the pJM14 integrative vector carrying the yxiD-yxxD flanking sites inserted using Gibson assembly kit from NEB. For inter-species competition L ...
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bioRxiv - Microbiology 2020Quote: ... Both mCherry and mEGFP were cloned with no linker after the Matrix domain in Gag via Gibson assembly with the HiFi DNA Assembly cloning kit (New England Biolabs). Similar constructs have been previously published ...
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bioRxiv - Plant Biology 2021Quote: ... The miR823-resistant CMT3 construct (rCMT3) was generated by PCR site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs) using the cCMT3 construct as a template to introduce six silent mutations as shown in Fig ...
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bioRxiv - Microbiology 2019Quote: ... cassette between SalI and BglII in the plasmid pGDB34 was isolated by digestion and gel purification and inserted between the same restriction sites in the plasmid pyPM2GT-EXP2-mNeonGreen14 with a T4 Quick Ligation kit (NEB), replacing the yDHODH cassette ...
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bioRxiv - Immunology 2021Quote: ... In vitro transcribed RNA was generated using the HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, Cat E2060S) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Illumina-compatible libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (catalog no. E7645S; New England BioLabs) and sequenced using an Illumina NovaSeq 6000 system in a paired-end 2x50- base pair (bp ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The extracted RNA was prepared for short-read sequencing library construction using the NEB NextUltra RNA-seq kit (New England Biolabs). The constructed libraries were sequenced with 150bp paired-end reads on an Illumina HiSeq 2500 and 6000 sequencing platforms ...
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bioRxiv - Immunology 2020Quote: ... 10ng or more of DNA was used to generate libraries according to the NEBNext RNA Library Prep Kit (New England Biolabs). Following the amplification step ...
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bioRxiv - Molecular Biology 2021Quote: ... gel-purified and cloned into SalI/SpeI-digested pAT006 (Tsuzuki et al., 2014) vector via HiFi DNAAssembly Cloning kit (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: ... The two fragments were cloned into AscI- digested pBYL2 vector (Mine et al., 2010) via HiFi DNA Assembly Cloning kit (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: ... Table S4) was cloned into XbaI/AscI-digested pBYL2 vector using the HiFi DNA Assembly Cloning kit (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: ... and then served as the DNA template for in vitro transcription reactions using the HiScribe T7 High Yield RNA Synthesis Kit (Cat. No. E2040S, NEB). Template DNA was removed by the addition of DNase I (Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... Paired-end libraries were prepared for four hog deer samples collected from Sunday Island in 2015 using a NebNext Ultra Library Prep Kit (BioLabs), with size selection of 500-600 base pairs (bp) ...
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bioRxiv - Molecular Biology 2020Quote: ... Wild-type (wt) gRNA was in vitro transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... mRNA was used to generate RNA-seq libraries using a NEBNext Ultra II RNA library prep kit for Illumina (New England Biolabs) following the manufacturer’s recommended protocol with the following parameters ...
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bioRxiv - Molecular Biology 2021Quote: ... Two biological replicates of RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) according the kit instructions ...
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bioRxiv - Immunology 2020Quote: ... RNA-seq libraries were constructed using the purified mRNA samples with NEBNext Ultra II Directional RNA library Prep Kit for Illumina (E7760S, NEB). The quality of these RNA-seq libraries were validated using 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Molecular Biology 2021Quote: ... EndoCβ-H1 and mouse islets was determined with an Agilent Bioanalyzer and mRNA enrichmed was performed from 500-1000ng of RNA using a NEBNext Poly(A) mRNA Magnetic Isolation Kit (NEB). Library preparation from mRNA and from miRISC-isolated RNA was performed using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Immunology 2021Quote: ... The enriched cDNA was converted to Illumina-compatible libraries using the NEBNext Ultra II DNA kit (New England Biolabs, Inc.) using 1ng input ...
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bioRxiv - Microbiology 2021Quote: ... and the DNA was repaired and A-tailed using the NEBNext DNA Library preparation kit for Illumina (New England Biolabs). After ligating Illumina compatible adapters to the DNA fragments ...
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bioRxiv - Immunology 2020Quote: ... and converted into RNA-seq libraries using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Mutations inducing amino acid substitutions in the vif region of infectious molecular clones or in the APOBEC3 expression constructs were introduced using Q5 site-directed mutagenesis kit (NEB). The presence of the desired mutations was confirmed by Sanger sequencing.
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bioRxiv - Immunology 2020Quote: ... We quantified RNA using a one-step RT qPCR reaction with the NEB Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs) and previously published TaqMan primers and probes (SARS-CoV-2 E_Sarbeco and hamster RPL18 ...
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bioRxiv - Microbiology 2021Quote: ... was used to make cDNA PCR templates from parasite RNA (Toxoplasma GT1; Plasmodium falciparum NF54) extracted with a Monarch Total RNA Miniprep Kit (New England Biolabs). Genomic DNA (Toxoplasma RH derivatives ...
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bioRxiv - Microbiology 2021Quote: ... A plasmid encoding an eGFP-containing EBOV antigenome was generated through assembly of fragments using the NEBuilder HiFi DNA assembly cloning kit (New England Biolabs (NEB)) ...
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bioRxiv - Molecular Biology 2021Quote: ... tropicalis pseudouridine synthase Pus7 was amplified from total RNA using the OneTaq One-step RT-PCR kit (New England Biolabs). XmaI and XhoI restriction sites were added with oligonucleotides and the resulting fragment was cloned into the p415Gal1 vector.
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bioRxiv - Immunology 2021Quote: ... and the RNA-seq libraries were generated with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Point mutations were introduced in the P and N sequences by site-directed mutagenesis using the Q5 site-directed mutagenesis Kit (New England Biolabs). Sequence analysis was carried out to check the integrity of all constructs ...
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bioRxiv - Microbiology 2020Quote: ... TEV was removed from the original plasmid and mEGFP was inserted in its place using a Gibson reaction via the HiFi DNA Assembly cloning kit (New England Biolabs).
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bioRxiv - Developmental Biology 2021Quote: ... each CBE4max-SpG and CBE4max-SpRY sequence has been inserted into pCS2+ plasmid linearized with EcoRI using the Gibson Assembly Cloning Kit (New England Biolabs). The fragment has been amplified using the primers F-5’-TGCAGGATCCCATCGATTCGGCCACCATGAAACGGACAG -3’ and R-5’-TAGAGGCTCGAGAGGCCTTGTCAGACTTTCCTCTTCTTCTTGG -3’ ...
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bioRxiv - Immunology 2021Quote: ... ii) fragmentation and adapter ligation (NEBNext® UltraTM II FS DNA Library Prep Kit for Illumina, NEB, cat. no. E7805S), and iii ...
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bioRxiv - Cell Biology 2021Quote: ... Stranded mRNA-Seq libraries were prepared from 250 ng of total RNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) implemented on the liquid handling robot Beckman i7 ...
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bioRxiv - Cell Biology 2021Quote: ... 100 ng of total RNA was used for library synthesis with the NEBNext Ultra II Directional RNA Library Preparation kit (New England Biolabs) according to the protocol of the manufacturer ...
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bioRxiv - Genomics 2021Quote: ... Libraries were prepared with the NEB Next® Ultra™ DNA Library Prep Kit (New England Biolabs, Ipswich MA, USA) and sequenced on an Illumina NovoSeq 6000 PE150 platform at Novogene (Hong Kong ...
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bioRxiv - Genomics 2021Quote: ... 25ng of DNA was used to prepare sequencing libraries using NEBNext® Ultra™ DNA Library Prep Kit (NEB, E7370L) according to manufactures manual ...
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bioRxiv - Genomics 2021Quote: ... 1 µg total RNA was subjected to cDNA synthesis applying the ProtoScript® First Strand cDNA Synthesis Kit (NEB, E6300S) with the included Oligo d(T)23 VN primer according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: All samples that met the previous criteria were submitted to cDNA synthesis protocol using LunaScript™ RT SuperMix Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: The poly(A) RNA library was obtained using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) including the NEBNext Poly(A ...
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bioRxiv - Genomics 2021Quote: The pCeTyX Ty1hygro-AI carrying plasmid was constructed by assembling four overlapping DNA fragments thanks to the Gibson Assembly Cloning Kit (NEB). The 5,472 bp plasmid backbone was obtained after restriction of p41Neo 1-F GW (https://www.addgene.org/58545/ ...
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bioRxiv - Genomics 2021Quote: ... The isolated mRNA was then used to prepare sequencing libraries using NEBNext Ultra II Non-directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (Index sets 1 and 2) (NEB). Next ...
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bioRxiv - Genomics 2020Quote: ... and 150ng of each sample were fed to library preparation with NEBNext Ultra II DNA Library Preparation kit for Illumina (New England BioLabs) according to manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: ... 30 seconds at 65°C and 30 seconds at 72°C using the NEBNext® Multiplex Oligos kit (NEB, UK). The expected library size was ∼200-250bp ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries were generated using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA), following the manufacturer’s recommendations ...
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bioRxiv - Genetics 2020Quote: ... Paired-end libraries were prepared by the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB, USA) and then sequenced on the Illumina HiSeq2500 platform after quantification ...
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bioRxiv - Genetics 2020Quote: ... Paired-end libraries were prepared by the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) and sequenced on the Illumina HiSeq2500 platform after quantification ...
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bioRxiv - Genetics 2020Quote: ... RNA sequencing libraries were prepared using the NEB Next Ultra RNA Library Prep Kit for Illumina using manufacturer’s instructions (NEB, USA). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... 1 kb of upstream and downstream DNA were cloned into the suicide vector pK18mobsacB by Gibson assembly45 using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). The vector was introduced into B ...