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Citations for New England Biolabs :
751 - 800 of 7437 citations for rno mir 155 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... or LunascriptTM RT (New England Biolabs) (sven5105-5107 variants) ...
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bioRxiv - Cell Biology 2021Quote: ... and AMV-RT (New England Biolabs) to assess U2-Um47 ...
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A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testingbioRxiv - Molecular Biology 2021Quote: ... 0.2 U/μl AMV RT (NEB), 4 U/μl SuperScript III (Thermofisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... Using LunaScript RT Supermix kit (BioLabs), cDNA was prepared in a 20 μL reaction according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... and RT enzyme mix (NEB #M0466), followed by an incubation step at 42°C for 90 minutes and then at 85°C for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... M-MLV RT buffer (NEB; B0253S) 1X ...
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bioRxiv - Developmental Biology 2023Quote: ... and RT enzyme mix (NEB, M0466) was combined with the annealed mixture ...
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bioRxiv - Cell Biology 2020Quote: ... Single-stranded DNA primers and primer dimers were digested using 1 unit μL−1 ExoI (New England Biolabs) and 1 U μL−1 HinFI (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We used primers specific to the RNA oligos and performed RACE analysis by nested RT-PCR using Q5 High-Fidelity DNA polymerase (New England BioLabs Japan Inc., Tokyo, Japan). The primers specific to dsx and dsx-like were made from sequences of the relevant genomic regions and are listed in supplementary table 9 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We used primers specific to the RNA oligos and performed RACE analysis by nested RT-PCR using Q5 High-Fidelity DNA polymerase (New England BioLabs Japan Inc., Tokyo, Japan). The primers specific to dsx and dsx-like were made from sequences of the relevant genomic regions and are listed in Table 6 ...
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bioRxiv - Microbiology 2022Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Frankfurt am Main, Germany) and a CFX96 Real-Time System ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative RT-PCR was conducted on a Roche Lightcycler 480 instrument using Luna Universal qPCR master mix (New England Biolabs in Ipswich, MA, USA). The geometric mean of recA and gyrB transcripts was used to normalise data ...
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bioRxiv - Microbiology 2021Quote: ... RT was performed using 1 μg of RNA using LunaScript™ RT SuperMix Kit (New England BioLabs) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was performed using Luna® Universal One-Step RT-qPCR kit (New England Biolabs; #E3005L). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using the Luna universal one-step RT-qPCR kit (#E3005, New England Biolabs) according to the provided protocol ...
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bioRxiv - Molecular Biology 2023Quote: RT-qPCR experiments were performed with the Luna® Universal One-Step RT-qPCR Kit (NEB, #E3005E) with a final reaction volume of 10 μL per well ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was performed using the Luna® SARS-CoV-2 RT-qPCR Multiplex Assay Kit (NEB) with the CDC-derived primers for N1 and N2 gene targets and the reaction was performed using the QuantStudio™ 5 System (ThermoFisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... and NEBNext Index primer (New England Biolabs). PCR products were purified again with the AMPure XP system and library quality was assessed on the Agilent Bioanalyzer 2100 ...
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bioRxiv - Systems Biology 2022Quote: ... 2.5 μl of Random primer mix (NEB) was added to the mixture then separated into 2 PCR tubes (one for -RT and one for +RT ...
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bioRxiv - Cell Biology 2019Quote: ... and NEBNext primers (New England Biolabs, E7600S), and subsequently size selected to 150-500 bp using SPRIselect beads (Beckman ...
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bioRxiv - Synthetic Biology 2020Quote: ... and Random Primer Mix (New England Biolabs) for quantification by RT-qPCR with Fast SYBR Green Master Mix (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL 10uM NEBNext Index Primer (NEB E7335 ...
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bioRxiv - Systems Biology 2021Quote: ... and oligo-dT primers (New England Biolabs). Quantitative real-time PCR was performed in triplicates using iTaq™Universal SYBR®Green reagent (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... and Oligo(dT)23 VN primer (NEB). A minus RT control was also performed using the highest total RNA amount (40 ng) ...
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bioRxiv - Neuroscience 2022Quote: ... dual index primers (New England Biolabs, #E7600S) were ligated in a library amplification step using 10 PCR cycles ...
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bioRxiv - Plant Biology 2022Quote: ... Primers used for NEBuilder Hifi assembly (NEB) contained 10-15nt overhangs at the 5’ end that was homologous to the 5’ end of another PCR product ...
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bioRxiv - Genetics 2024Quote: ... phosphorylating the primers using T4 PNK (NEB) and digesting and ligating the insert into the pX458 plasmid using BbsI (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... dual index primers (New England Biolabs, #E7600S) were ligated in a library amplification step using 10 PCR cycles ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL 10 µM RP1 primer (NEB), 1 µL ddH2O and PCR amplified for 16-18 cycles ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL 10 µM RPI primer (NEB), 2 µL 10 µM RP1 primer (NEB) ...
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bioRxiv - Biophysics 2024Quote: ... and oligo-dT primers (New England Biolabs). Quantitative real-time PCR was performed in triplicates using iTaqUniversal SYBRGreen reagent (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: ... A PCR fragment containing two sgRNA target sequences was generated using the pCBC-DT1T2 vector template with the Phusion polymerase (NEB; See Table S6 for primers), and the purified PCR fragment was then cloned into pBEE401E using a golden gate reaction with BsaI (Wang et al ...
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bioRxiv - Microbiology 2024Quote: ... or a region in the N-terminal part (for TGDs) were PCR amplified (see Table S4 for primer sequences) from 3D7 or IT4 gDNA (Monarch gDNA Purification Kit, NEB or QIAGEN DNA extraction kit) and cloned into pSLI (Birnbaum et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... The reaction was set up using a commercial kit (PURExpress NEB) according to user guidelines ...
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bioRxiv - Developmental Biology 2021Quote: ... NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs) was used for library preparation according to manufacturer’s protocol with some changes ...
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bioRxiv - Genomics 2019Quote: The NEBNext Small RNA Library Prep Set for Illumina (NEB, E7300) was used following manufacturers’ instructions with small modifications as follows ...
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bioRxiv - Genomics 2022Quote: ... NEBNext® Small RNA Library Prep Set for Illumina® (NEB), a standard in-house ligation-based cloning protocol [36] ...
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bioRxiv - Microbiology 2021Quote: ... qPCR reactions were set-up using Luna qPCR Master Mix (NEB) following the manufacturer’s protocol ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... one set of the replicates were treated with lambda phosphatase (NEB) overnight at 25°C before incubation with the pS1615 antibody (Figure 2 – figure supplement 1B).
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bioRxiv - Molecular Biology 2024Quote: ... and NEB MultiplexOligo sets I/II/III/IV (NewEngland Biolabs, E7500L) without size selection according to the manufacturer’s protocol with modifications ...
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bioRxiv - Genomics 2022Quote: ... The RNA samples and standards were amplified using the Luna® Universal Probe One-Step RT-PCR Kit (E3006, BioLabs®Inc, Ipswich, Massachusetts, USA), with 0.6 mM primer and 0.2 mM probe concentrations (Table S4) ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR on extracted RNA was performed using the Luna One-Step RT-qPCR Kit (New England Biolabs). The samples were analyzed using a Lightcycler 480 instrument (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... One-step RT-qPCR was performed using the Luna universal One-Step RT-qPCR kit (New England BioLabs) in a total volume of 20 µl and a template concentration of 50 ng/µl according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Beads were reinjected at 75 μl/h and co-flowed with 150 μl/h RT mix (1.65x Maxima H-RT Buffer, 0.033 U/μl USER II (NEB), 15 U/μl Maxima H-RT (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: One-step RT-qPCR was performed using the Luna universal One-Step RT-qPCR kit (New England Biolabs) in a total volume of 10 µl and a template concentration of 50 ng/µl according to manufacturer’s recommendation ...
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bioRxiv - Bioengineering 2023Quote: ... The RT-RPA was performed at 42°C for 30 min by combining M-MuLV-RT (NEB #M0253L) with TwistAmp Basic (TwistDx #TABAS03KIT) ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA synthesis and RT-qPCR was carried out using Luna® Universal One-Step RT-qPCR Kit (NEB) according to the manufacturer’s instructions with 100 ng of mRNA ...
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bioRxiv - Genomics 2020Quote: ... centered on the candidate miR-122 ORF site was cloned with NEB HiFi DNA Assembly Master Mix (New England Biolabs) into the open reading frame of firefly luciferase in pmirGLO ...
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bioRxiv - Genomics 2019Quote: ... cDNA was synthesized using MaximaH RT (NEB) enzyme following manufacturer’s protocol and oligoDT primers ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 U M-MuLV RT (NEB) in RT Buffer (25 mM KCl ...