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Citations for New England Biolabs :
751 - 800 of 1699 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... and 1 μL of BaeGI-HF (NEB; 100,000 units/mL) and incubated for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... and G5E4 DNA or 0.2 ml of AvaII (NEB, R0153L) for the 12×601 with various linker lengths and incubated at 37°C for 24 hours (except for the Tetra-nucleosome 601 DNA) ...
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bioRxiv - Biophysics 2023Quote: ... 1 mM TCEP and 2.5 units/mL of YIPP (NEB). At each time point reaction was quenched with 4X LDS and 50 mM DTT then run on SDS-PAGE ...
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bioRxiv - Biophysics 2023Quote: ... 1 mM TCEP and 2.5 units/mL of YIPP (NEB). RNA-nsp9 product was then purified from the protein and leftover RNA components through a 1 mL HiTrap Q Fast Flow column in a buffer of 20 mM HEPES pH 7.5 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 0.5 µL T4 DNA Ligase (NEB, 400,000 U/mL) were used with 1 µL T4 DNA Ligase Reaction Buffer (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... 20 mL ERA reaction (13 T4 DNA ligase buffer [NEB] ...
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bioRxiv - Microbiology 2023Quote: ... and was loaded onto 2 mL of chitin resin (NEB) pre-equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... Six microliters of proteinase K (20 mg/ml) (NEB, P8107S) were added ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 5.5 mL of Rnase A (NEB, Cat. T3018L) and 5.5 mL of RNase If (NEB ...
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bioRxiv - Genetics 2024Quote: ... 10 µL 10 mg/mL BSA (NEB cat no. B9000s), 840 μL DNA grade H2O (Invitrogen Waltham ...
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bioRxiv - Microbiology 2023Quote: ... Six microliters of proteinase K (20 mg/ml) (NEB, P8107S) were added ...
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bioRxiv - Cancer Biology 2024Quote: ... or 5,000 U/mL PNGase F (P0704S, New England BioLabs) in growth media for 1 hour at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA-bound beads were incubated in 100 μl end-repair mix containing 1x T4 Ligase Buffer (NEB), 0.5 mM dNTP mix ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.0001ng of pUC19 plasmid methylated at 100% of CpG sites (New England BioLabs, Ipswich, MA, USA) were used as spike-in controls to determine the efficiency of APOBEC deamination and TET2 oxidation ...
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bioRxiv - Genomics 2022Quote: ... The mixture electroporated into 100 μL of 10-beta E.coli (NEB, C3020K, 2kV, 200 ohm, 25 μF) in order to achieve a transformation efficiency equal to 300-times the number of unique oligos sequences (target CFU ...
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bioRxiv - Genomics 2020Quote: ... the beads were equilibrated once in 100 μL 1x NEB Quick Ligation Reaction Buffer (New England Biolabs). Then the beads were resuspended again in 50 μL 1x NEB Quick Ligation Reaction Buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... the resin was resuspended in 100 μL of NLS Buffer and 10 μL of Proteinase K (NEB) and the sample was incubated at 50°C for 30 minutes while shaking at 1200 rpm ...
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bioRxiv - Molecular Biology 2019Quote: Five pmol of each standard was dephosphorylated in a 100 μL reaction of 1× CutSmart Buffer (NEB) with 5 units of calf intestinal alkaline phosphatase (CIP ...
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bioRxiv - Biophysics 2021Quote: ... Reactions of 100 µL volume were prepared using the PURExpress Δ Ribosome Kit (New England Biolabs, E3313S) containing 0.8 U/µL of SuperASE-In ...
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bioRxiv - Biophysics 2021Quote: ... The λ-DNA reagents are prepared starting with 2 µg of commercially prepared DNA incubated with 25 units of Nt.BbvCI and Nb.BssSI to a final volume of 100 µL in 1X 3.1 buffer (New England Biolabs). Nicking of genomic E ...
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bioRxiv - Genomics 2019Quote: ... and deaminated with 100 U of APOBEC3A (EM-seq components E7133AA and E7134AA NEB, Ipswich, MA, USA) in 100 µl reaction volume for 3 h ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 ng of total RNA was used to prepare cDNA using ProtoScript II Reverse Transcriptase (NEB #M0368) with oligo(dT ...
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bioRxiv - Biochemistry 2020Quote: ... 100 ng of gel purified DNA was used as a template for in vitro transcription (NEB E2040S) carried out according to the manufacturer’s instructions with the addition of 0.1µl of Cy3 (Sigma PA53026 ...
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bioRxiv - Synthetic Biology 2021Quote: ... before gel extraction (0.8 % agarose gel, gel green dye, 80V, 100 minutes; T1020S, New England Biolabs Monarch). Plasmid backbone (50 fmol ...
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Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery systembioRxiv - Genetics 2019Quote: ... 1 µL of each oligo (100 µM) were mixed with 1 µL of T4 ligation buffer (NEB) and 7 µL of water ...
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bioRxiv - Genomics 2021Quote: ... The digested sample was diluted with 400 µL T4 ligation buffer (NEB, M0202, 1% Triton X-100) and incubated at 37°C for 30 min ...
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bioRxiv - Genomics 2021Quote: ... mRNA was isolated from 100 ng of total RNA per sample using oligo dT magnetic beads (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Immunoprecipitation was performed overnight under rotation at 4 using 1/100 T7RNA antibody (Biolabs CB MAB-0296MC) and antiflag (Sigma F1804 and F3165) ...
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bioRxiv - Cell Biology 2022Quote: ... On the third day cells were stained with 100 nM SNAP-Cell TMR-Star (New England BioLabs) for 30 min at 37°C and chemically fixed with a mixture of 4% paraformaldehyde and 0.1% glutaraldehyde in 0.2 M HEPES buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... we incubated single cell with 100 nL of end repair mix (1.6 nL Klenow Large Fragment (NEB), 1.6 nL T4 PNK (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... pellets dried and resuspended in 100 µL RNase-free water containing 40 U murine RNase inhibitor (NEB). Following quantification by Nanodrop ...
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bioRxiv - Biophysics 2023Quote: ... concentrated protein was supplemented with 100 U TEV-His7 protease in 1X TEV protease reaction buffer (NEB) and incubated at 30 °C with agitation for 3 hr to cleave the His6-tag ...
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bioRxiv - Zoology 2023Quote: ... The amplified products were measured using a 100 base pair (bp) DNA ladder (New England Biolabs Inc.) at specific bands corresponding to their base pairs for the two bacterial species.
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bioRxiv - Genomics 2023Quote: ... PCR master mix (64 µL of H2O, 100 µL of Q5 high-fidelity DNA polymerase (NEB #M0544S), 8 µL of i5 primer ...
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bioRxiv - Biochemistry 2023Quote: ... PCR reactions were purified using GeneAid and ∼100 ng of DNA subjected to digestion by NaeI (NEB). Clones that showed no apparent digestion product (due to the modification of the NaeI restriction site ...
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bioRxiv - Genomics 2022Quote: ... The pellet was resuspended in 1 mL of NEB and centrifuged at 11,000rpm for 20 min on a sucrose gradient (0.65 mL of 1.6 M sucrose in NEB, 0.35 mL of 0.8 M sucrose in NEB). The Nuclei pellet was then resuspended in 1 mL of NEB ...
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB, 45% glycerol 80% and 30% dH2O). and incubated for 30 min at 37°C and for 15 min at 80°C ...
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bioRxiv - Neuroscience 2020Quote: ... The nuclei-containing pellets were resuspended in 1 mL of NEB + 0,25 M sucrose and centrifuged at 20000xg for 20 min on sucrose gradient (0.65 mL of 1.6 M sucrose in NEB, 0.35 mL of 0.8 M sucrose in NEB). The pellet was resuspended in 1 mL of NEB and formaldehyde to a final concentration of 1% ...
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bioRxiv - Cell Biology 2023Quote: ... 250 μL of 2 mg mL-1 Y289L GalT,46 50 μL of 500 kU mL-1 PNGase F (New England Biolabs, 5 U μg-1 of protein), and 62.5 μL of Lambda Protein Phosphatase (New England Biolabs ...
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bioRxiv - Genomics 2020Quote: ... The supernatant was loaded on a 10-mL chitin column (NEB). The column was washed with HEGX ...
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bioRxiv - Bioengineering 2022Quote: ... 1 μL of 2-Log DNA Ladder (200 μg/mL; NEB) is mixed with 1 μL of Gel Loading Dye (6x ...
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bioRxiv - Cell Biology 2022Quote: ... 1% SDS) containing 400 μg/mL Proteinase K (New England Biolabs) and incubated at 65 °C for 1 hr with rotation at 300 RPM ...
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bioRxiv - Cell Biology 2022Quote: ... and 3 μL of 20 mg/mL Proteinase K (NEB EO0491) was added to each sample ...
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bioRxiv - Cell Biology 2022Quote: ... 4 μL of 20 mg/mL Proteinase K (New England BioLabs) was added and incubated for 1 h at 55 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... in HBSS supplemented with 10 µg/ml DNase (New England Biolabs) at 37°C and swirled at 100 rpm for 32 minutes to enzymatically separate the epithelium from the mesenchyme as we previously did [47] ...
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bioRxiv - Genetics 2020Quote: ... supplemented with 10 μl Proteinase K (800 U/ml, NEB #P8107), and incubated at 55°C for 14-18h while shaking at 800rpm ...
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bioRxiv - Genomics 2019Quote: ... AluI (New England Biolabs R0137 at 0.015 mg/ml; MW = 46,000) was added (0 ...
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bioRxiv - Developmental Biology 2020Quote: ... ATP was depleted with 4.5 U/ml apyrase (New England Biolabs) for 10 min at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... and then passed over an 5-8 mL amylose column (NEB). The bound protein was washed with ten CV of Amylose Wash Buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... and 375 U/mL of Klenow Fragment (3’-5’ exo-) (NEB). After cDNA synthesis and subsequent purification by AMPure XP (Beckman Coulter) ...