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Citations for New England Biolabs :
751 - 800 of 1314 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... was incubated with 10 U/ul Lambda Protein Phosphatase(New England Biolabs, #P0753) at 30°C for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Molecular weights were determined using protein standards (New England Biolabs P7712, or P7719). Puromycin incorporation assays were performed essentially as described in (38) ...
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bioRxiv - Cell Biology 2021Quote: ... and biotin-pupE labelled proteins were enriched by streptavidin magnetic beads (NEB, S1420S) and identified by mass spectrometry analysis (Liu et al. ...
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bioRxiv - Biochemistry 2020Quote: All proteins were expressed in Escherichia coli NiCo21 (DE3) cells (New England BioLabs). To co-express multiple proteins ...
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bioRxiv - Biochemistry 2023Quote: ... the REC114-MEI4 protein-containing supernatants were loaded onto an Amylose resin (NEB). After extensive washing with a buffer containing 20mM Tris pH 8 ...
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bioRxiv - Developmental Biology 2023Quote: ... 2ng gRNA was injected together with 1.9nM Cas9 protein (New England Biolabs M0386T) and 10% Phenol Red (Sigma- Aldrich P0290 ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein A magnetic beads (Cat#S1425S, New England Biolabs Inc, Ipswich, MA, USA) were aliquoted (20 □l ...
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bioRxiv - Developmental Biology 2023Quote: ... The two gRNAs were injected together with Cas9-NLS protein (New England Biolabs) into one-cell stage embryos ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted by the addition of 1uL TEV Protease (New England Biolabs) and incubated for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted proteins were loaded onto a 6 mL amylose column (New England Biolabs), washed in 50 mM HEPES-KOH (pH 7.5) ...
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bioRxiv - Cell Biology 2024Quote: Proteins were expressed in Escherichia coli T7 Express lysY/Iqcells (New England Biolabs) in 2×TY medium at 37°C to an OD600 of 0.8–1.2 before inducing protein expression by addition of 0.4 mM isopropyl β-D-thiogalactopyranoside (IPTG) ...
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bioRxiv - Immunology 2023Quote: ... either 1.8 mg USP2 or 800 U Lambda protein phosphatase (New England BioLabs) was added as indicated ...
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bioRxiv - Biochemistry 2024Quote: ... the protein bound RNA was dephosphorylated with Quick CIP (M0525S, New England Biolabs) for 10 min at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... The MBP-fused protein was purified with Amylose Resin (E8021V; New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... ∼50 μg of protein were incubated with 20 units of λPP (NEB, USA) for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... following the final wash proteins were eluted in Glycoprotein Denaturing Buffer (NEB, P0702S) for 10 mins at 100°C and then resuspended in EndoH (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... 4.2 ng/μl extreme thermostable single-stranded DNA binding protein (New England Biolabs) and 0.02 U/μl SuperFi DNA polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... cross-linked virion samples were deglycosylated using Protein Deglycosylation Mix II (P6044, NEB) under denaturing conditions according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... DHFR control plasmid from PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) was used as backbone for subsequent ligations ...
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bioRxiv - Developmental Biology 2023Quote: ... Freshly laid wild-type TU-strain embryos were injected with SpCas9 protein (NEB) mixed with gRNAs (~300 ng/ul) ...
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bioRxiv - Biochemistry 2023Quote: ... and (MBP)2-HSPC300 proteins were purified through amylose beads (New England Biolabs). The Sra1/Nap1 dimer was obtained by co-expressing His6-Sra1 and untagged Nap1 in Tni insect cells (Expression Systems) ...
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bioRxiv - Cell Biology 2024Quote: ... protein samples were incubated with 500 U of PNGase F (New England Biolabs) or Endo H (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... The Color Protein Standard Broad Range (10–250 kDa) (New England BioLabs Inc.) was used as protein molecular weight ladder ...
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bioRxiv - Biophysics 2024Quote: ... The lysate was pre-cleared using protein G magnetic beads (New England Biolabs). The cleared supernatant was then incubated with 3 µg anti-myc antibody (SIGMA M4439 monoclonal anti c-myc or Abcam ab206486 rat mAb to myc tag (9E10) ...
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bioRxiv - Synthetic Biology 2024Quote: ... expression was performed using the PURExpress® In Vitro Protein Synthesis Kit (NEB). For the non-T7 constructs ...
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bioRxiv - Biochemistry 2024Quote: ... We then dephosphorylated HRI using Lambda Protein Phosphatase (Lambda PP (New England Biolabs)) ...
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bioRxiv - Genetics 2024Quote: ... Immunoprecipitation was performed using protein A magnetic beads (New England BioLabs, Ipswich, MA). The beads were washed ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was expressed in Shuffle T7 Escherichia coli strain (New England Biolabs) for expression ...
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bioRxiv - Genetics 2024Quote: ... the protein was mixed with Quick Calf Intestinal Alkaline Phosphatase (New England BioLabs) for 1 hour at room temperature to be dephosphorylated ...
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bioRxiv - Cell Biology 2020Quote: ... Full-length 265 nt 5’ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...
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bioRxiv - Immunology 2021Quote: ... PCR products were purified and cloned into human-IgG (Heavy, Kappa or Lambda) expression plasmids(von Boehmer et al., 2016) using the Gibson Assembly Master Mix (NEB) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Library preparation from human islets total RNA (200ng) was performed using a NEBNext Low Input RNA Library Prep kit (NEB). Sequencing was performed on a HiSeq4000 using 75 bp paired end reads according to Illumina specifications ...
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bioRxiv - Molecular Biology 2021Quote: ... Full-length 265 nt 5′ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...
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bioRxiv - Bioengineering 2021Quote: ... were synthesized (Genewiz) based on the GenBank sequences with regions of overlap to restriction digested human IgG1 vectors for assembly cloning (NEB) to produce plasmids ...
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bioRxiv - Biochemistry 2020Quote: ... the human Rab1b 3-174aa (referred to as Rab1b)-encoding DNA was cloned into a modified pMAL vector (New England Biolabs), resulting in a construct with a N-terminal hexahistidine (6xHis ...
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bioRxiv - Cell Biology 2021Quote: The entry vector pDONR223 containing the full-length GRN (1–1179 base pairs) from the human orfeome collection was used to engineer a stop codon by site-directed mutagenesis (New England Biolabs) at the end of the open-reading frame sequence (ORF) ...
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bioRxiv - Immunology 2020Quote: ... and the ratio of viral genome: human gDNA were estimated using qPCR via Luna Universal qPCR Master Mix (New England Biolabs) using designed primers Gag-Fwd ...
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bioRxiv - Biochemistry 2021Quote: ... were synthesized (Genewiz) based on the GenBank sequences with regions of overlap to restriction digested human IgG1 vectors for assembly cloning (NEB) to produce plasmids ...
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bioRxiv - Cancer Biology 2022Quote: ... The plasmid for human UBE2C overexpression was made by subcloning the PCR-amplified UBE2C (IDT) fragment into the EcoRI and Mscl (NEB) site of LeGO-iV2.
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bioRxiv - Developmental Biology 2020Quote: ... We tested all primers using 1uL of genomic DNA from H9 human ES cells in a PCR reaction containing 12.5 uL Phusion High Fidelity PCR Master Mix (NEB, M0531L), 1.25 uL 5 uM forward primer ...
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bioRxiv - Developmental Biology 2020Quote: ... We previously generated a human LRP5-V5 plasmid [32] and we used the Q5 site-directed mutagenesis kit (New England Biolabs) with forward primer (CTCAATGGCACATCCCGG ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μg of total RNA was depleted of rRNA using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) prior to cDNA synthesis primed with random hexanucleotide oligonucleotides (Random Primer 6, NEB). Sequencing library construction was carried out using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Cell Biology 2020Quote: ... A fragment of human Plexin-B1 cDNA (encoding amino acids 1-535) was cloned into the pcDNA5 vector using HindIII (R0104S, NEB) and XhoI (R0146S ...
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bioRxiv - Biochemistry 2021Quote: ... per 1g of wet cell weight and 1 Unit of 30 Units/μL RNase Inhibitor (from human placenta; New England Biolabs (NEB), No ...
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bioRxiv - Immunology 2021Quote: ... PCR products were purified and cloned into human-IgG (Heavy, Kappa or Lambda) expression plasmids(von Boehmer et al., 2016) using the Gibson Assembly Master Mix (NEB) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: The HRAS minigene reporter was constructed by PCR amplifying a ∼900bp region spanning exon 4 to exon 6 from human genomic DNA isolated from HEK293 cells using Phusion high- fidelity DNA polymerase (NEB). The PCR fragment were inserted into pcDNA3.1(+ ...
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bioRxiv - Developmental Biology 2023Quote: ... The two strands of the gRNA were annealed and cloned downstream of the human U6 promoter using the BbsI (NEB) restriction site in the plasmid pU6-(BbsI)_CBh-Cas9-T2A-BFP (Addgene) ...
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bioRxiv - Synthetic Biology 2023Quote: ... low passage HEK293T cells were co-transfected a dual Cas9 and gRNA expression vector (pX330-T2) targeting the human AAVS1 locus71 and a PmeI-linearized (NEB) repair template comprising a constitutively expressed YFP-HygR-expression cassette with a BxB1 attP recognition site flanked by 800bp homology arms for the human AAVS1 locus (pROC079) ...
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bioRxiv - Developmental Biology 2023Quote: ... full length coding sequences were amplified from human cell line cDNA with AttB overhangs using Phusion High-Fidelity DNA polymerase (M0530S, New England BioLabs) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and Illumina linker addition to B cell heavy chain transcripts were performed using the human NEBNext Immune Sequencing Kit (New England Biolabs) according to the manufacturer’s instructions ...