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Citations for New England Biolabs :
751 - 800 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... A second digestion was performed by resuspending previous 3C DNA template in 1X RE buffer and incubating with NlaIII (NEB, R0125) overnight at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... The region around the miossa22946 allele was amplified for 35 cycles using 60°C for annealing and the mutant allele was identified by restriction enzyme digestion with DdeI for 1 hour (New England BioLabs, R0175S). The genomic region around the miossa20 allele was amplified for 35 cycles using 57°C for annealing and identified by restriction enzyme digestion of the wild-type allele with BsaWI for 1 hour (New England BioLabs) ...
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bioRxiv - Genomics 2023Quote: ... Each tested enhancer sequence was cloned into the corresponding dual-enSERT-1 vector using NotI digestion followed by Gibson assembly methods (NEB, E2611) (Gibson et al. ...
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bioRxiv - Neuroscience 2023Quote: ... (see the sequence map in Figure 1) was subjected to restriction digestion using two restriction enzymes: XbaI and AccI (New England Biolabs (NEB)) in the cutsmart buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Positive clones were selected on ampicillin-containing plates and checked by the consequent release of correct-sized fragments following double digestion with NdeI and BamHI (New England Biolabs Inc.). Both clones were confirmed by sequencing.
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bioRxiv - Biochemistry 2023Quote: ... containing plates and checked by the consequent release of correct-sized fragments following double digestion with NdeI and BamHI (New England Biolabs Inc.). All the clones were confirmed by sequencing.
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... Oligonucleotides were ligated into pSp-Cas9-(BB)-2A-GFP plasmid that had been linearised by digestion with BbsI-HF (NEB R3539) and gel purified (Bioline BIO-52060 ...
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bioRxiv - Molecular Biology 2023Quote: F1 dumpy and/or non-dumpy animals were isolated from dumpy jackpot plates and genotyped by PCR and restriction digestion using HpyCH4IV (F180Δ, NEB R0619), HinfI (G199S ...
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bioRxiv - Neuroscience 2023Quote: ... The vector was prepared for oligonucleotide ligation by digestion with SanDI (KflI, Thermo ScientificTM FD2164) and dephosphorylation with CIP (NEB, M0525). The ligation was performed with a vector/sponge insert ratio of 1:300 at 16 °C for 1h ...
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bioRxiv - Biochemistry 2024Quote: ... containing plates and checked by the consequent release of correct-sized fragments following double digestion with NdeI and BamHI (New England Biolabs Inc.). All the clones were confirmed by sequencing.
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bioRxiv - Biochemistry 2023Quote: ... The DNA bound to the beads was made ss by λ exonuclease digestion of the strand with 5’ phosphate (5 U/mg DNA, NEB). The reaction was carried out at 370 C for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... Digestion products were diluted by the addition of 4 mL 1.1× T4 DNA Ligase Reaction Buffer (New England BioLabs® Inc) with 1% Triton X-100 and incubated for 1 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... The resulting double-stranded DNA fragments were individually cloned into pJMP1339 following digestion with BsaI (New England Biolabs Cat. No. R3733), purification (Monarch PCR & DNA Cleanup Kit ...
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bioRxiv - Microbiology 2023Quote: ... The S RNA MG used was cloned to include the full-length GPC and an mCherry gene replacing the NP gene by digestion with AvrII (NEB, R0174S), then insertion gene fragments with NEBuilder HIFI DNA Assembly Mix (NEB ...
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bioRxiv - Genetics 2024Quote: ... 250 ng of RNA samples were digested at 37°C for 2 hours with Nucleoside Digestion Mix (New England Biolabs, M069S). Digested RNA samples were diluted to 100 µl with double-distilled water and filtered through 0.22 µm Millex Syringe Filters ...
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bioRxiv - Microbiology 2024Quote: ... The pBAD33 plasmid harboring the stem structure of rrsH (corresponding to -131∼-102 and +1559∼+1589 relative to +1 of rrsH gene) under T7 promoter was linearized by digestion with XbaI (NEB, R0145).
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bioRxiv - Plant Biology 2021Quote: Purified LPR1 protein was analyzed using Protein Deglycosylation Mix II (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Protein (200 µg) was precleared with protein G magnetic beads (New England Biolabs) and incubated overnight (4° C ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleaved G protein was dephosphorylated by lambda protein phosphatase (NEB, Frankfurt, Germany), calf intestinal phosphatase (NEB ...
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bioRxiv - Microbiology 2023Quote: ... The protein ladder used was Colour Protein Standard Broad Range (NEB, Ipswich, US).
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bioRxiv - Plant Biology 2024Quote: ... 2µg of recombinant protein was mixed with 400U lambda protein phosphatase (NEB, #P0753S) at 30°C for 30 min before separated by an SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: In vitro trans-translation assays were performed using the PURExpress In Vitro Protein Synthesis and Δ Ribosome kits (New England Biolabs). For trans-translation assays ...
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bioRxiv - Plant Biology 2020Quote: 20-33 kD subregions of NRPD1 and RDR2 polypeptides were expressed in vitro using a PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... The SARS-CoV-2 Spike mutant D614G was generated by site-directed mutagenesis using as an input DNA the expression vector encoding SARS-CoV-2 Spike_614D protein (kindly provided by J. Garcia-Arriaza, CNB-CSIC) by Q5 Site Directed Mutagenesis Kit (New England Biolabs, Barcelona, Spain) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... 200-500 ng of the purified PCR product was used as DNA template in 25 μl of coupled in vitro transcription and translation reaction using PURExpress In Vitro Protein Synthesis Kit (New England Biolabs, E6800L). The reaction was incubated at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... for each co-expressed DF-APOBEC1 protein using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760L) with NEBNext Poly(A ...
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bioRxiv - Synthetic Biology 2023Quote: ... we constructed the genetic sequence of the major shell protein of 50nmGVs using Q5 DNA polymerase and Gibson assembly kits (New England Biolabs Inc.)65 ...
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bioRxiv - Cell Biology 2022Quote: ... unique protospacers targeting the 50-bp window surrounding the 1st codon of each gene were annealed and cloned into the pCAB_minimal via BbsI digestion and ligation (New England BioLabs # R3539S, Takara #6023) using standard protocols 48 ...
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bioRxiv - Molecular Biology 2021Quote: ... The plasmids were mixed in equimolar ratio and 20μg of mixed plasmid was subjected to restriction digestion with the enzyme Sau3AI (NEB, Catalog number: R0169) (Isoschizomer of DpnII ...
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bioRxiv - Plant Biology 2021Quote: ... The cloning reaction consists of 25 cycles of 3 min at 37°C for digestion and 4 min at 16°C for ligation combining the restriction enzymes BsaI-HF (New England BioLabs, Ipswich, UK) for level 1 reaction or BpiI (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... 50µl of lysed cells were transferred to a tube containing 450µL of digestion mix (1X NEB 1 buffer, 1% triton X-100). This process was repeated 11 times (total 12 tubes with 500 µl) ...
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bioRxiv - Genomics 2022Quote: ... Chimeric RNA barcode molecules were eluted from the bead by incubating with 127 µL ProK digestion solution (11 µL ProK (NEB cat # P8107B),100 mM Tris pH 7.5 ...
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bioRxiv - Physiology 2022Quote: ... from epithelium or sensory neurons underwent digestion for 10 hours at 37°C with MspI restriction enzyme (New England Biolabs, Ipswich, MA), followed by purification with AMPure XP (Beckman Coulter ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was purified using phenol/chloroform precipitation and the second digestion was performed by adding 400U of NlaIII restriction enzyme (NEB Cat#R0125S), then incubation at 65°C for 4h ...
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bioRxiv - Microbiology 2021Quote: ... We transferred the plug to 5 ml of fresh dilute TE in a clean tube for storage until we performed a ß-agarase I digestion of the agarose and isopropanol DNA precipitation according to the manufacturer’s instructions (New England Biolabs, Ipswich, MA). We resuspended the precipitated DNA in 100 µl EB (10mM Tris-HCL ...
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bioRxiv - Genetics 2022Quote: ... Strains expressing exogenous Dbp1 from the LEU2 locus were created by cloning the DBP1 ORF as well as ∼1kb of upstream and downstream regulatory sequence into a single integration plasmid targeted to LEU2 This plasmid was linearized by digestion with SwaI nuclease (NEB, Ipswich, MA) and transformed into the dbp1Δ::KANMX6 strain.
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bioRxiv - Molecular Biology 2019Quote: ... DNA substrates used for digestion reactions were either generated by PCR using Q5® High-Fidelity 2X Hot Start Master Mix (NEB #M0494), oligonucleotides produced by IDT ...
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bioRxiv - Plant Biology 2020Quote: ... and the quality was checked by digestion of 250 ng of genomic DNA from 10 random samples with the restriction enzyme EcoRI (New England Biolabs, Boston, MA) at 65°C for 2 hour and thereafter visualised on agarose gel.
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bioRxiv - Plant Biology 2021Quote: ... benthamiana IMPa-4 fragment were used for genomic PCR and followed by digestion with BamHI and XhoI (New England Biolabs, Ipswich, MA). The pTRV2 vector (ABRC ...
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ER exit sites in Drosophila display abundant ER-Golgi vesicles and pearled tubes but no megacarriersbioRxiv - Cell Biology 2021Quote: ... Gel purified PCR product soo-XbaI-Gateway-XbaI-soo was then cloned into pTGA linearized by XbaI digestion (New England Biolabs, cat#R0145S) using SoSoo mix reagent (TreliefTM SoSoo Cloning Kit ...
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ER exit sites in Drosophila display abundant ER-Golgi vesicles and pearled tubes but no megacarriersbioRxiv - Cell Biology 2021Quote: ... which added NheI and SpeI restriction sites for subsequent double enzyme digestion and ligation with T4 DNA ligase (New England Biolabs, cat # M0202L) to obtain pTSA-Tango1 ...
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bioRxiv - Molecular Biology 2021Quote: ... XIST genomic regions were PCR amplified from the genomic DNA extracted from NT2/D1cells using specific primer pairs (Supp Table 1) followed by restriction enzyme digestion of the vector and insert and ligation using T4 DNA ligase (New England Biolabs, MA, USA). The positive clones were confirmed by sequencing.
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bioRxiv - Biochemistry 2022Quote: ... A 25 μg aliquot of CP-treated CTDNA was diluted to 100 μL of 1X nucleoside digestion mix reaction buffer (NEB, Ipswich, MA) at pH 5.4 and treated with 2.5 μL NEB nucleoside digestion mix (1 μL or 1 U per 10 μg CTDNA ...
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bioRxiv - Molecular Biology 2024Quote: Genomic phage DNA was digested using either a combined treatment of DNaseI and ExoI to create single nucleotides or a commercially available nucleoside digestion mix (NEB, Ipswich, USA).
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bioRxiv - Molecular Biology 2023Quote: ... was immersed in a 10-fold volume of digestion solution with 8 U/mL Proteinase K (P8107S, New England Biolabs, Ipswich, USA) in digestion buffer containing 50 mM Tris pH 8.0 ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: ... Lentiviral related PLD3/4 plasmids were cloned into pBOBI vector that has a C-terminal FLAG tag after digestion by restriction enzymes BamHI and XhoI (NEB R3136S, R0146S). All these plasmids were prepared with endotoxin-free kit (Takara 740426 ...
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bioRxiv - Biochemistry 2024Quote: ... Digestion was set up to remove the 6X-His-tag by incubating with either homemade or store bought (NEB, Cat. no: P8070L) enterokinase in the presence of 10mM CaCl2 at 22°C for 16h ...
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bioRxiv - Biophysics 2024Quote: ... Transcription templates were prepared by PCR using primers complementary to the T7 promoter and terminator sequences (see Supplementary Table 1) or by digestion of the plasmid DNA with SphI (NEB, Cat# R3182S). mRNA fragments were prepared by in vitro transcription with T7 RNA polymerase using a ratio of 10:30 AU:GC NTPs ...
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bioRxiv - Neuroscience 2021Quote: ... Protein molecular ladder (Color Prestained Protein Standard, Broad Range 11–245 kDa, NEB, P7712S). Dry transfer was performed using an iBlot™ 2 gel transfer device (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... purified proteins were directly digested by O-glycosidase (P0733, NEB, 4,000 units/µg protein) and α2-3 ...