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Citations for New England Biolabs :
751 - 800 of 1204 citations for GATOR2 complex protein WDR59 WDR59 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The DST was removed by incubating the protein with 64 units of Enterokinase light chain (BioLabs) in 10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: Genetically modified zebrafish lines were created using Cas9 protein (EnGen® Spy Cas9 NLS, #M0646T, NEB) and previously established protocols combining PCR and reverse transcription to generate guide RNAs44 ...
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bioRxiv - Cell Biology 2024Quote: ... AcGFP and TagRFP-657 proteins and SNAP-Surface 488 and Alexa 647 dyes (New England Biolabs) were measured in the same excitation and emission ranges as in flow cytometry assays ...
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Novel RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteriabioRxiv - Molecular Biology 2024Quote: ... In vitro translation was performed with the PURExpress in vitro protein synthesis kit (New England Biolabs). The 10 µL PURExpress reactions with or without 2 µM YebC variants were preincubated at 37°C for 5 min ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant protein of AP2 domains fused with MBP (MBP::PbSIP2) was purified using amylose resin (NEB) and recovered in 10 mM maltose solution.
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bioRxiv - Microbiology 2024Quote: ... Twenty µg of protein was treated with PNGase F as per manufacturer instructions (New England Biolabs). Water was used as the mock treatment ...
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bioRxiv - Microbiology 2024Quote: ... we digested a lentiviral plasmid that contains mCherry in the location where the G viral entry protein is cloned (4016_V5LP_pHrU3_ForInd-Extgag_mcherry) with XbaI and MluI-HF (NEB, Cat ...
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bioRxiv - Neuroscience 2024Quote: ... and used to form ribonucleoprotein (RNP) complexes when combined with recombinant Cas9 protein (New England Biolabs). Ribonucleoprotein complexes were formed using the method of Kouranova49 ...
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bioRxiv - Biochemistry 2024Quote: ... reactions were performed with 6 μl of PURExpress in vitro protein synthesis system (New England Biolabs). The reactions were carried out on different templates (Supplementary Table 2) ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were eluted with lysis buffer + 250 mM imidazole and bound to chitin-resin (S6651L, NEB), before washing in lysis buffer and overnight incubation with lysis buffer + 200 mM DTT and his-ULP1 protease ...
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bioRxiv - Bioengineering 2024Quote: Protein was expressed for in vitro assays in T7 Express cells (New England Biolabs, Catalog # C2566H) in 96-well deep-well plates using the pET22b(+ ...
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bioRxiv - Molecular Biology 2024Quote: ... MBP-fusion proteins were affinity purified with amylose resin following the manufacturer’s instructions (New England Biolabs). The identity and purity of recombinant purified MBP-fusion proteins were confirmed by SDS-PAGE and western immunoblotting analyses.
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bioRxiv - Biochemistry 2024Quote: ... Alkaline phosphatase treatment was performed over night at 4°C using Lambda Protein Phosphatase (#P0753S, BioLabs).
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was then incubated with amylose affinity resin (NEB, 2mL bead volume/1L bacterial culture) for 1hour at 4°C while nutating ...
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bioRxiv - Biophysics 2024Quote: ... The protein was then SNAP labeled in 2x molar excess of SNAP-Surface AlexaFluor 647 (NEB) and incubated at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2024Quote: ... All reactions were performed in duplicate using PUREexpress in vitro protein synthesis reagents (New England Biolabs) supplemented with 1 uL of Murine RNase inhibitor (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... into vectors containing a fluorescent protein and a selection cassette using Gibson Assembly (New England Biolabs), as thoroughly detailed by Dickinson et al ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified proteins were pre-treated (were indicated) with PNGase F or Endo H (New England Biolabs, USA) according to the manufacturer’s protocol before analysis by Western blotting using Strep-MAb-HRP classic antibody.
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bioRxiv - Molecular Biology 2021Quote: ... 20 μg of protein lysates of each condition was treated with either Endo H (New England Biolabs) or PNGase F for 1 h at 37º C ...
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bioRxiv - Physiology 2020Quote: ... 1 nl of CRISPR mixture containing 2,4 μg/μl of gRNA and 0.5 μl Cas9 protein (NEB) was injected in one-cell stage embryos and raised for 24 hours ...
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bioRxiv - Plant Biology 2021Quote: ... protein pellets were dissolved in 20 μl of PNGase F denaturation buffer (New England Biolabs, Ipswich, MA) and denatured at 95°C for 10 min ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were separated by SDS-PAGE and detected by immunoblotting using anti-MBP (New England BioLabs, E8032S), anti-GST (MBL ...
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bioRxiv - Biochemistry 2020Quote: ... treated with lambda phosphatase protein after purification at 30°C for 1 hr (New England BioLabs, P0753L), or co-expressed in BL21 cells with RFP-lambda phosphatase ...
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bioRxiv - Biochemistry 2021Quote: 20 µg protein lysate was incubated with lambda phosphatase according to the manufacturer’s protocol (New England Biolabs). Afterwards ...
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bioRxiv - Biochemistry 2020Quote: 1 μg of N protein was dissolved in 36 μL H218O and 2 μL 10x glycobuffer (NEB). To this solution 2 μL PNGase F was added and the reaction mixture was incubated at 37 °C for 16 h ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were loaded directly onto the gel along with a pre-stained protein marker (New England Biolabs), and were run with a constant voltage of 60 V ...
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bioRxiv - Molecular Biology 2022Quote: ... Removal of fucose was performed using α1-2,4,5,6 fucosidase O (New England Biolabs, 2 U/μg protein), and α1-3,4 fucosidase (New England Biolabs ...
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bioRxiv - Plant Biology 2022Quote: ... His-TAD1 fusion proteins were purified according to the manufacturer’s protocol (New England Biolabs, Ipswich, MA, USA). The bait protein GST-WL1 was incubated with GST beads (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: Recombinant condensin II holo(WT) at 500 nM was mixed with or without λ protein phosphatase (NEB) at a final concentration of 400 U/µL in 1x NEBuffer for Protein MetalloPhosphatases supplemented with 1 mM MnCl2 and incubated at 30°C for 60 min ...
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bioRxiv - Genetics 2021Quote: Total protein was extracted from dissociated cultured cells by incubating in 1x RIPA buffer (New England Biolabs) with added MS-SAFE Protease and Phosphatase Inhibitor (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... proteins are recovered by digesting the DNA with the PtsI restriction enzyme (New England Biolabs, Ipswich, MA), whose cut site was incorporated into all designed oligos.
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bioRxiv - Synthetic Biology 2021Quote: ... Protein expression plasmids were cloned using Gibson Assembly (NEB Gibson Assembly Master Mix, New England Biolabs #E2611) into a pET-28c plasmid backbone and were designed to overexpress recombinant proteins as C-terminus His-tagged fusions ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 μg EBNA2 or 130 μg GST-fused EBNA2 protein were incubate in 1x PK buffer (NEB, 50 mM Tris-HCl ...
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bioRxiv - Genomics 2021Quote: ... followed by adapter and motor protein ligation onto the prepared ends using NEB ligase (New England Biolabs). Subsequently ...
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bioRxiv - Biochemistry 2021Quote: ... with and without the addition of ACE2 (~1:1.25 S-protein trimer:ACE2 molar ratio) (New England Biolabs). Vitrified samples of S-protein constructs with and without ACE2 were prepared by first glow discharging Quantifoil R1.2/1.3 300 mesh holey carbon copper grids for 1 minute using a Pelco easiGlow glow discharge unit (Ted Pella ...
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bioRxiv - Cell Biology 2020Quote: ... Bacterial lysates were prepared and the fusion protein purified over a chitin binding column (NEB labs, UK). HA-Ub75-thioester was obtained by incubating the column material with mercaptosulfonate sodium salt (MESNa ...
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bioRxiv - Cancer Biology 2021Quote: ... Bacterial lysates were prepared and the fusion protein purified over a chitin binding column (NEB labs, UK). HA-Ub75- thioester was obtained by incubating the column material with mercaptosulfonate sodium salt (MESNa ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were prediluted to a final concentration of 750 nM in 1x CutSmart buffer (New England Biolabs) and incubated with the respective guide at 37°C for 15 min in a 1:1 ratio ...
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bioRxiv - Genetics 2023Quote: ... After mixing 5μM~10μM sgRNAs of bmp6a and bmp6b and 5μM Cas9 protein (M0646, NEB, MA, USA), 1nL mixture was micro-injected into single-cell embryos ...
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bioRxiv - Genetics 2022Quote: ... except in the absence of phosphatase Inhibitor and incubated with 400u lambda protein phosphatase (New England Biolabs) at 30°C for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... Bound protein was detected by western blot using mouse anti-MBP diluted 1:10,000 (New England Biolabs), fluorescent secondary antibodies (Li-Cor Biosciences) ...
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bioRxiv - Plant Biology 2023Quote: ... and recombinant MBP-tagged proteins purification were performed according to the manufacturer’s instructions of Amylose Resin (NEB).
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bioRxiv - Cell Biology 2023Quote: ... 20μg protein was denatured following manufacturer’s protocol followed by PNGase F and O-Glycosidase & Neuraminidase Bundle (NEB) treatment at 37°C for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... Cascade complexes were purified via the N-terminal maltose binding protein (MBP) tag using amylose beads (NEB) and eluted with lysis buffer containing 10 mM maltose ...
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bioRxiv - Cell Biology 2023Quote: ... 1-cell stage embryos were injected with two guides for each gene with Cas9 protein (NEB, M0646T). Tyrosinase was an injection control to validate the efficacy of the Cas9 protein ...
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bioRxiv - Biophysics 2023Quote: ... concentrated protein was supplemented with 100 U TEV-His7 protease in 1X TEV protease reaction buffer (NEB) and incubated at 30 °C with agitation for 3 hr to cleave the His6-tag ...
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bioRxiv - Biochemistry 2024Quote: ... The strep tags were removed by incubating the proteins with 64 units of Enterokinase light chain (BioLabs) in 10 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... 1.4 μM of each purified mRNA was used in PURExpress in vitro Protein Synthesis Kit (NEB#E6800) in 10 μl reaction in 384 well Small Volume black plate (Grenier 788 096) ...
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bioRxiv - Microbiology 2024Quote: ... and plasmid pQE70-TstrepSACOL2603myc-2602-2601HA-hisTspA for protein purification were created using HiFi assembly (NEB, E0554S). SACOL2603 ...
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bioRxiv - Molecular Biology 2023Quote: MBP-Nla6 DBD was purified according to instructions in the pMALTM Protein Fusion & Purification System manual (NEB). Briefly ...