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Citations for New England Biolabs :
751 - 800 of 3658 citations for Diethyl 2 chlorothiazol 5 yl methylphosphonate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Coli NEB® 5-alpha aliquot (NEB®, #C2987H). Plasmids were sanger sequenced by GeneWiz (Azenta Life Sciences ...
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bioRxiv - Biochemistry 2024Quote: ... Coli NEB® 5-alpha aliquot (NEB®, #C2987H). Plasmids were sanger sequenced by GeneWiz (Azenta Life Sciences ...
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bioRxiv - Synthetic Biology 2024Quote: ... 5 µl of 10x T4 ligase buffer (NEB B0202S), 1 µl of T4 PNK ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 5 units of polymerase (New England Biolabs M0273). Vent reactions were performed in 1X Thermopol buffer (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... and 5 µM Mth RNA Ligase (New England Biolabs). The 100 µl master mix was split into 4 aliquots (25 µl each ...
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bioRxiv - Genomics 2024Quote: ... 5 µl 10x NEBuffer2 and 1 µl M.SssI (NEB) in a total volume of 50 µl (topped off with ddH2O) ...
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bioRxiv - Genetics 2024Quote: ... 5 µL of Exonuclease I Reaction Buffer (NEB, B0293S) was mixed with 1 µL 1:200 diluted UltraPure BSA solution (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... 5 µL of Lambda Exonuclease Reaction Buffer (NEB, B0262S) was mixed with 1 µL of nuclease-free water ...
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bioRxiv - Cell Biology 2024Quote: 5-alpha Competent E.coli High Efficiency (NEB, Cat# C2987I)
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bioRxiv - Cell Biology 2021Quote: ... RHA PCR products were ligated into pAAV p21 vector by Gibson assembly at a ratio vector:inserts of 1:2:2 using T4 DNA ligase (NEB). All constructs were checked by sequencing before transfection into cells ...
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bioRxiv - Biochemistry 2021Quote: ... sDrl-2 for crystallization was also partially deglycosylated with PNGase F (New England BioLabs: 2,000 unit/mg sDrl-2) for 3 h at room temperature before sizing.
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bioRxiv - Biochemistry 2021Quote: ... 2 μl of this lysate was directly used for PCR reactions with 2× Taq start master mix (M0496L, NEB), and the two NASP clone screening primers (see primer table) ...
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bioRxiv - Synthetic Biology 2022Quote: ... These were phosphorylated (2 μL 100 μM oligo stock, 2 μL 10X T4 DNA ligase buffer (New England Biolabs), 1 μL T4 PNK (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 nM dT adaptor primer and 200 nM Vλ1-GSP4-2-Hind III and 2 U Taq polymerase (NEB), in a final volume of 50 µl ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μl each of two complementary oligonucleotides (Mut_sgRNA_F and Mut_sgRNA_R) (Supplementary file 1) at a concentration of 20 μM and 2 μl of NEBuffer 2 (NEB B7002S) were added into an Eppendorf tube ...
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bioRxiv - Microbiology 2024Quote: ... The gel-extracted nascent RNA in 5 µl nuclease-free water was ligated to 10.7 pmol barcode DNA linker (Supplementary Table 2) using 200 U truncated T4 RNA ligase 2 (NEB) overnight at 16°C ...
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bioRxiv - Neuroscience 2020Quote: ... with TdTomato was amplified by PCR with primers (5’- ggcgcgCCCCCCTCTCCCTCCCCCCC -3’ and 5’- ggcgcgccTTACTTGTACAGCTCGTCCATGCCGTACAG -3’) using Hot start Q5® polymerase (NEB, Frankfurt, Germany) from LeGO-iT (a gift from Boris Fehse ...
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bioRxiv - Microbiology 2021Quote: ... was utilized using SARS-CoV-2 specific primers[43] or SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (New England Biolabs), which became available in assays after September 15 ...
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bioRxiv - Biophysics 2020Quote: For the insertion of an ATTO647N-labeled oligonucleotide complementary to the position 14711 bp from the biotinylated end of the λ DNA we employed the previously described strategy14 and followed the more recently described procedure.15 2 μg of λ DNA was incubated for 2 hours with the nicking enzyme Nt.BstNBI (20 units, NEB) at 50 °C in the nickase buffer ...
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bioRxiv - Neuroscience 2021Quote: ... PCR product (2 μg) and pEYFP-C1 vector DNA (2 μg) were digested with BamHI-HF and HindIII-HF (New England Biolabs) according to the manufacturer ...
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bioRxiv - Synthetic Biology 2021Quote: ... The annealing was carried out in 96-well PCR plates by mixing 2 μL of each oligonucleotide at 100 μM with 2 μL of 10x T4 DNA Ligase Reaction Buffer (NEB) and 14 μL of water ...
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bioRxiv - Systems Biology 2021Quote: ... PCR reactions with plasmid and genomic DNA templates were performed using the Phusion High-Fidelity 2× Master Mix or Q5 High-Fidelity 2× Master Mix (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... was amplified and barcoded in parallel using the same PCR program in 50 μL PCR reaction (2 μL gap-repaired DNA, 25 μL 2× NEBNext High-Fidelity PCR Master Mix (NEB), 1.5 μL 10 μM i5 universal PCR primer ...
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bioRxiv - Synthetic Biology 2023Quote: ... we first mixed 10.5 uL of purified RNA with 2 uL of 2 uM RT primer (AGGGACATCGTAGAGAGTCGTACTTANNNNNNNNNNAGATGAACTTCAGGGTCAGC, where Ns comprise the UMI) and 2uL of 10mM dNTP mixture (NEB), incubated the mixture at 65C for 5 minutes ...
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bioRxiv - Microbiology 2022Quote: ... human NINL isoform 2 (NCBI accession NM_001318226.2) and the NINL isoform 2 mutant (Q231R) were mutagenized using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). The plasmids encoding 3C proteases (coxsackievirus B3 (CVB3 ...
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bioRxiv - Cell Biology 2023Quote: ... A repair template for editing endogenous plk-2 gene to express PLK-2::3xFLAG::TIR1CIP was assembled into a pCR-Blunt backbone using Gibson Assembly (NEB) and confirmed using Sanger sequencing and whole-plasmid Nanopore sequencing (Primordium) ...
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bioRxiv - Molecular Biology 2021Quote: ... 41.7 µL of Phusion 2× Master Mix (NEB), 2.5 µL of 10 µM primer mix (515F forward and 806R reverse rRNA gene V4 primers with Illumina MiSeq adaptors) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... treated with 2 units of DNase I (NEB) at 37°C for 1 hour followed by DNase I heat inactivation ...
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bioRxiv - Genomics 2020Quote: ... 2 μl Antarctic Phosphatase [5k U/mL] (NEB) and 1 μl RNase inhibitor and incubating at 37 °C for 30 minutes with shaking ...
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bioRxiv - Genomics 2021Quote: ... 10 µL of 2% BSA (New England Biolabs), 930 µL of nuclease-free water and supplemented with 0.2 U/ul RNaseIN Plus (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl RNase Inhibitor (80U final, NEB M0307L) and 2 µl SuperScript III (400U final ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl RNase Inhibitor (80U final, NEB M0307L) and 1 µl AMV RT (12U final ...
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bioRxiv - Genetics 2021Quote: ... viewpoint 2: 10X NEBuffer™ DpnII (R0543M, NEB)) and 15 μl of 10% SDS buffer were added to the 450 μl sample ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 2 μl 10x CutSmart buffer (New England Biolabs), 10 μl shrimp alkaline phosphatase (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... 2 μl 10× T4 RNA ligase buffer (NEB), 20 U T4 RNA ligase enzyme ...
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bioRxiv - Microbiology 2021Quote: ... 2 μl of 10× Antarctic Phosphatase buffer (NEB), 1 U Antarctic Phosphatase ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 µl T4 ligase (NEB cat #M0202M). For Capture-C ...
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bioRxiv - Molecular Biology 2022Quote: ... and 2 μl T4 Ligase Buffer (NEB, B0202) in a total volume of 30 μl for 18 hours at 16 °C ...
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bioRxiv - Immunology 2022Quote: ... dNTP mix (2 mM each) (New England Biolabs), 50U Superscript III RTase ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 units of T4 polynucleotide kinase (NEB, M0201S), 0.09 nM dNTPs and 0.045 μg/μL of BSA at 12°C for 30 min ...
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bioRxiv - Plant Biology 2021Quote: ... M-TM2^2 was digested with BslI (NEB) for 3 hours at 55°C and M-2 was digested with XhoI (NEB ...
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bioRxiv - Biophysics 2021Quote: ... 2 nM M13mp18 circular ssDNA (New England Biolabs) (Table S3 ...
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bioRxiv - Synthetic Biology 2022Quote: ... added Cut smart (2 ul, Cat # B7204; NEB), Bsa I (2ul ...
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bioRxiv - Microbiology 2022Quote: ... 2 μl of RNaseH (10 U, NEB, M0297) was added followed by an incubation of 20 minutes at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl 10x NEBuffer 1 (New England Biolabs), 2 μl 50 mM MnCl2 ...
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bioRxiv - Microbiology 2021Quote: ... and Vaccinia 2’-O-methyltransferase (New England Biolabs). The mRNA was purified by oligo-dT affinity purification ...
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bioRxiv - Neuroscience 2021Quote: ... 2) proteinase K digestion (New England Biolabs P8107S) at 37°C for 18 hrs ...
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bioRxiv - Microbiology 2021Quote: ... 2 μl murine RNase inhibitor (New England Biolabs) in nuclease assay buffer (40 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... 2 μl murine RNase inhibitor (New England Biolabs) in nuclease assay buffer (40 mM Tris-HCl ...
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bioRxiv - Neuroscience 2020Quote: ... 2 μL PNGase F (500U/μL) (NEB # P0704) was added to the elution buffer and the samples were incubated for 3 h at 37 °C ...