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Citations for New England Biolabs :
751 - 800 of 1384 citations for 8 Bromo 3 iodo quinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: dATP was added to the 3’ ends by adding 9μL of A-tailing mix (5μL NEB buffer 2.1 ...
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bioRxiv - Genomics 2021Quote: ... followed by an overnight incubation at 16°C with 3 µL T4 DNA ligase (NEB, M0202). Samples were purified with phenol-chloroform and used as 3C templates for Taqman-qPCR ...
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bioRxiv - Biochemistry 2021Quote: ... Concentrated medium was collected from the filters and 3 μl of PNGase F (New England Biolabs) was added to each sample then incubated at 37 °C for 24 h ...
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bioRxiv - Biochemistry 2021Quote: ... media was replaced with media containing 3 µM SNAP-Cell block (New England BioLabs cat. # S9106S) and cells maintained at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Table 3) region of clpP1 was amplified by PCR (GC buffer, Phusion polymerase, New England Biolabs) using isolated L ...
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bioRxiv - Microbiology 2021Quote: ... 5’ triphosphorylated RNA was capped with 3’-desthiobiotin-TEG-guanosine 5’ triphosphate (DTBGTP) (New England Biolabs) using the vaccinia capping enzyme (VCE ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA fragments were end-repaired and A-Tailed using Klenow fragment (3’-5-exo-) (NEB, M0212L), the DNA fragments were ligated to illumina adaptors (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... 3′ dA-tailed using NEBNext® Ultra™ II End Repair/dA-Tailing Module (NEB E7546L) and were purified with paramagnetic beads ...
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bioRxiv - Genetics 2021Quote: ... Singlet and duplet pools were A-tailed using using Klenow HC 3’→5’ exo- (#M0212L; NEB).
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bioRxiv - Cancer Biology 2020Quote: ... Positive control slides were treated with 3 U/mL DNase-1 (New England Biolabs, Cat. M0303) for 10 minutes at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... and a NEBNext Multiplex Oligos for Illumina (Index Primers Set 3) (Code E7710; New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... denatured and phosphorylated with 10 U of 3’-phosphatase-minus T4 polynucleotides kinase (New England Biolabs) at 37 °C for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... and ligated to the 3′ adapter by incubating with T4 RNA ligase 2 truncated mutant (NEB) and 1 µg of pre-adenylated adapter (5′rAppCTGTAGGCACCATCAAT/3ddc ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A deoxyadenosine triphosphate was added at each 3’end with the Klenow fragment (New England Biolabs), and (3 ...
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bioRxiv - Neuroscience 2023Quote: ... 3 μg of total RNA was reverse transcribed using LunaScript cDNA Synthesis Kit (New England Biolabs). Gene-expression levels were quantified by real-time quantitative PCR (iCycler iQ BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... The tRNA 2’-3’ cyclic phosphate was removed by treatment with T4 PNK (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... then dATP was added to the 3′ ends of the DNA using Klenow exo-(NEB #M0212). The DNA was then subjected to double-sided SPRI bead size selection (AMPure XP beads ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A deoxyadenosine triphosphate was added at each 3’end with the Klenow fragment (New England Biolabs). Illumina Truseq adapters (Affymetrix ...
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bioRxiv - Molecular Biology 2023Quote: ... 3-4 μg of sperm gDNA was dephosphorylated with 10 μl of rSAP (NEB, no. M0371S) and 16 μl of 10× rCutSmart buffer (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Pre-adenylated L3-1R-App 3’ adaptors were ligated using T4 RNA ligase 1 (M0204, NEB) for 75 min at 25°C ...
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bioRxiv - Biophysics 2022Quote: ... The gene sequences were flanked by restriction enzyme sites for 5’ NdeI and 3’ EcoRI (NEB). Genes were cloned into pET21a using standard protocols from NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’ and 3’ homology arms and the insert were cloned into the pUC19 vector (NEB, #N3041S) by using Gibson Assembly Master Mix (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Buffer IIIb (3% Triton X-100, 1x T4 ligase buffer, 1x protease inhibitors; NEB, Sigma-Aldrich) was added to a final volume of 1 ml and sample was incubated for 15 min at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... About 3 µg of DNA from each sample was digested with two restriction enzymes (PstI; NEB catalog #R0140 and XhoI ...
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bioRxiv - Bioengineering 2024Quote: ... The extracted DNA (3 μg) was fully digested with NcoI restriction enzyme (New England Biolabs, USA), separated in a 0.8% agarose gel and blotted onto a Hybond+ Nylon membrane (Amersham Biosciences) ...
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bioRxiv - Cell Biology 2024Quote: ... newly deposited CENP-A was labelled with 3 µM SNAP-Cell® 647-siR (S910102S, NEB) for 30 mins before washing excess with media and allowing to grow for a further 30 mins ...
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bioRxiv - Genomics 2024Quote: ... and pooled into 7 ml ligation buffer (1X ligation buffer 3 (New England Biolabs; without ATP), 1 mM ATP ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcoded RNA-Seq libraries were prepared with 200 ng of total RNA with RIN > 8 using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). Poly A+ RNA was purified using poly-T oligo-attached magnetic beads followed by fragmentation and first and second cDNA strand synthesis ...
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bioRxiv - Cancer Biology 2021Quote: ... a barcoded RNA adapter (RiL 19) was ligated to the 3’ end using T4 RNA ligase (NEB). The 3’ adaptor ligated RNA was again cleaned up as mentioned above ...
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bioRxiv - Cancer Biology 2021Quote: ... ΔN1/2/3 and ΔDAD mutants were generated by Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) with HOXB13 WT or NCOR1 WT as a template ...
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bioRxiv - Microbiology 2021Quote: ... After dephosphorylation of the 3′ ends of the recovered RNA using T4 polynucleotide kinase (T4 PNK; NEB), RNA was ligated to Linker-1 (5′-App CTGTAGGCACCATCAAT ddC-d′ ...
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bioRxiv - Molecular Biology 2021Quote: ... Mutations on smaller fragments of the RBP10 3′-UTR were done using either site directed mutagenesis (NEB, Q5® Site-Directed Mutagenesis Kit Quick Protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Mutations on smaller fragments of the RBP10 3’-UTR were done using either site directed mutagenesis (NEB, Q5® Site-Directed Mutagenesis Kit Quick Protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... A preadenylated universal linker (5’-rAppCTGTAGGCACCATCAAT-NH2-3’) was prepared in house or purchased from NEB (S1315S) and ligated to the 3’ ends of the dephosphorylated footprints using T4 RNA Ligase 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were washed, treated with T4 Polynucleotide Kinase (PNK, with no 3’-phosphatase activity) (NEB, M0236S), and miRNA-RNA chimeras were produced with T4 RNA Ligase I (NEB ...
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bioRxiv - Microbiology 2019Quote: ... The fragment of ENV was excised from pNL4-3(AD8) using BamHI and EcoRI restriction enzymes (NEB) and ligated using T4 ligase (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... Co-precipitated RNA was dephosphorylated and a 3’ RNA linker was ligated using T4 RNA ligase (NEB). The AATF-RNA complexes and input controls were run on a NuPage bis-tris protein gel ...
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bioRxiv - Molecular Biology 2019Quote: ... Subsequently 5’ and 3’ termini were ligated using 10 units of T4 RNA ligase (New England Biolabs) at 37°C for 1 h ...
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bioRxiv - Cancer Biology 2020Quote: ... two oligos (see Supplementary Table 3) were annealed and 5’ phosphorylated (T4 Polynucleotide Kinase kit, M0201S, NEB) as described previously (LentiGuide-Puro and LentiCRISPRv2) ...
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bioRxiv - Biophysics 2021Quote: ... and 3 samples from each replicate combined into 7 mL with 1 × T4 DNA Ligase Buffer (NEB), with 1% Triton X-100 ...
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m7G cap-eIF4E interaction stimulates polysome formation by enhancing first-round initiation kineticsbioRxiv - Biophysics 2021Quote: ... 5’-end capping and 3’-end biotinylation were performed using the Vaccinia Capping System (New England Biolabs) and the Pierce RNA 3’ End Biotinylation Kit (Thermo Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... 3 µl Antarctic phosphatase [5 U] and 7.32 µl Antarctic phosphatase buffer (both New England BioLabs, Germany). The reaction was heat-inactivated at 85°C during 15 min ...
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bioRxiv - Genomics 2022Quote: Beads were resuspended in 80 µl T4 PNK reaction mix (3 µl T4 PNK (NEB cat# M0201B), 97.2 mM Tris pH 7 ...
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bioRxiv - Microbiology 2022Quote: ... as follows: 1µL of 2µM MBTUni-12 primer (5’-ACGCGTGATCAGCRAAAGCAGG-3’) + 1µL 10mM dNTPs Mix (NEB #N0447S) + 8µL Nuclease-free water (Ambion ...
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bioRxiv - Microbiology 2022Quote: ... 23 nt RNA (5’-GAAUCUAUACAUAAAGACCAGGC-3’) was capped with vaccinia capping enzyme and 2’-O-methyltransferase (NEB) and radiolabelled with [α 32P]-GTP ...
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bioRxiv - Microbiology 2020Quote: The 3′ ends of the ribosome footprint RNA fragments were then treated with T4 polynucleotide kinase (NEB) to allow ligation of a pre-adenylated DNA linker with T4 Rnl2(tr ...
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bioRxiv - Microbiology 2019Quote: ... 10 µg of RNA was incubated with 3 units of DNase I (New England Biolabs, MA, USA) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Cleaned up RNA was then ligated to 3’ pre-adenylated DNA adaptor (NEB, sequence see Table S4) by T4 RNA ligase 2 ...
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bioRxiv - Genomics 2019Quote: ... Ligation was performed at a 1:3 ratio of pJDrcEPP:library using T4 DNA ligase (New England Biolabs). Ligation product was cleaned-up using a Clean and Concentrator Kit (Zymo Research ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA fragments were directly 3’-end dephosphorylated using 5 U of Antarctic Phosphatase (New England Biolabs, UK) for 30 min at 37°C ...