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Citations for New England Biolabs :
7801 - 7850 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Up to 1ug of RNA is then converted into cDNA using the NEB LunaScript® RT Supermix Kit (NEB #E3010) followed by qPCR using the NEB Luna Universal qPCR Master Mix (NEB #M3003) ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA of 2×106 cells was extracted with the Monarch Total RNA Miniprep Kit with “on column” DNase I treatment (NEB). cDNA was generated by using LunaScript RT SuperMix Kit (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were polyA-enriched and directional libraries were prepared using the NEBNext Ultra II Directional Library Prep Kit for Illumina (NEB). Libraries were PE150 sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... the NEBNext Ultra II directional RNA library prep kit with the NEBNext Poly(A) mRNA magnetic isolation module (New England Biolabs) was utilized according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The preparation of sequencing libraries for ribosome profiling was conducted via the NEBNext Multiplex Small RNA Library Prep kit for Illumina according to the manufacturer’s protocol (NEB, #E7300S). Pair-end sequencing reads of size 150 bp were produced for Ribo-seq on the Ilumina Hiseq X-ten system ...
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bioRxiv - Neuroscience 2024Quote: ... Around 5 ng of purified CUT&RUN-enriched DNA was used to prepare NGS libraries using the NEBNext Ultra II DNA Library Prep Kit (NEB) with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... then 40 μL of each sample was used reactions as outlined in the manufacturer’s protocol for denaturing reaction conditions using the Protein Deglycosylation Mix II Kit (New England Biolabs, P6044) both with and without deglycosylase enzymes ...
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation was performed using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760S/L). The workflow was adapted to generate longer library fragments by reducing the fragmentation time (see manual ...
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bioRxiv - Molecular Biology 2024Quote: ... After washing samples were resuspended in 150 µl RNA cleanup binding buffer of the Monarch RNA Cleanup Kit (NEB, T2030S). Samples were incubated for 5 min at RT on a rotating wheel ...
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bioRxiv - Neuroscience 2024Quote: ... Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversely transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530 ...
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bioRxiv - Molecular Biology 2024Quote: ... These three DNA fragments were further assembled using the NEBuilder HiFi DNA Assembly cloning kit (New England BioLabs, Ipswich, MA).
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bioRxiv - Molecular Biology 2024Quote: ... Modifications of uORFs or other mutations were performed using the Q5 site-directed mutagenesis kit (Cat. No. E0554S, New England Biolabs) and validated by Sanger sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... ChIP-seq libraries were generated using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB # E7645S) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 cycles on a Covaris E220 focused ultrasonicator and were subsequently processed using NEBNext Enzymatic Methyl-Seq kit (NEB #7120) following the manufacturer protocol ...
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bioRxiv - Biochemistry 2024Quote: ... a codon-optimized synthetic gene product was synthesized in two overlapping halves by Twist Biosciences and integrated into a vector using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB). pNH-TrxT was a gift from Opher Gileadi (Addgene plasmid # 26106 ...
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bioRxiv - Cancer Biology 2024Quote: ... Guide RNAs were generated in house using Highscribe™ T7 Quick High Yield RNA synthesis kit (New England Biolabs Inc.), Alt-R™ S.p ...
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bioRxiv - Pathology 2024Quote: ... cDNA library was generated from total mRNA (1 µg) using NEBNext UltraTM RNA Library Prep Kits for Illumina (New England BioLabs). cDNA libraries were sequenced by NovaSeq 6000 (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... graminearum or mock inoculated was treated with DNAse and cDNA was synthesized using LunaSCript RT MasterMix kit (New England Biolabs), with random hexamer and oligo-dT primers ...
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bioRxiv - Neuroscience 2024Quote: ... The enriched mRNA was fragmented into short fragments using fragmentation buffer and reverse transcribed into cDNA using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530 ...
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bioRxiv - Physiology 2024Quote: ... DNA libraries were generated as per the manufacturer’s protocol using the NEBNext® Poly(A) mRNA Magnetic Isolation Module kit (New England Biolabs). 1μg total RNA per sample was inputted and adaptor ligation and PCR enrichment was performed using the NEBNext® Multiplex Oligos for Illumina® kit as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... IVT was performed following the manufacturer’s protocol for the HiScribe T7 High Yield RNA Synthesis Kit (NEB, Ipswich, MA, USA). Polyadenylated RNA was added to the IVT RNA control at a ratio of 1:1000.
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bioRxiv - Neuroscience 2024Quote: ... The four separate fragments were amplified and inserted into a vector containing the Tol2 sites35 using the Gibson Assembly Cloning Kit (New England Biolabs).
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bioRxiv - Molecular Biology 2024Quote: ... The 3′ adapter was conjugated with an amino CA linker instead of dCC at the 3′ end (GeneDesign) and then adenylated at the 5′ end with a 5′ DNA adenylation kit (NEB). Four random nucleotides were added in the 3′ and 5′ adapters [(5′ -rAppNNNNTGGAATTCTCGGGTGCCAAGG/amino CA linker-3′ ...
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bioRxiv - Biochemistry 2024Quote: ... 1-5 mL of cell pellet was harvested for plasmid extraction with the Monarch Plasmid DNA Miniprep Kit (New England Biolabs) (T1010) ...
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bioRxiv - Biochemistry 2024Quote: ... with C-terminal TEV, GFP, 7xHis, and StrepII (Thawani et al., 2020) using the Q5 Site Directed Mutagenesis Kit (NEB). All three constructs were expressed using the Bac-to-Bac Sf9 cell expression system (Invitrogen) ...
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bioRxiv - Genomics 2024Quote: ... TruSeq-barcoded RNAseq libraries were generated with the NEBNext Ultra II Non-Directional RNA Library Prep Kit (New England Biolabs). Each library was quantified with a Qubit (dsDNA HS kit ...
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bioRxiv - Developmental Biology 2024Quote: ... Construction of pUAST- attB-Cic(1-15)A-SBP and pUAST-attB-Cic20A-mVenus was carried out by assembling gene fragments using the HiFi DNA assembly Kit (NEB). Construction of the other subgroups of Cic mutants was done by assembling different parts of Cic generated by overlap PCR using wild type Cic ...
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bioRxiv - Biochemistry 2024Quote: ... was introduced into linearized pEGFP-C1 ΔSV40-Stop with Xho I and BamH I use the HiFi DNA Assembly Cloning Kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 5 ng of ChIP and Input DNA was used to generate ChIP-seq libraries using the NEBNext Ultra DNA library prep kit for Illumina (New England Biolabs, NEB).
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bioRxiv - Genomics 2024Quote: ... Following end repair with NEBNext® End Prep Ultra II Kit (Supplemental Table 9, Cat No. E7645, NEB, Ipswich, MA), custom iTru adapters were ligated to amplicons with NEBNext® Ultra II Ligation Master Mix (Cat No ...
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bioRxiv - Biochemistry 2024Quote: ... Of the rRNA-depleted samples directional libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) in accordance to the recommended protocol ...
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bioRxiv - Biophysics 2024Quote: ... Plasmid DNA was extracted using the Qiagen Plasmid Plus Midi kit and the library inserts were obtained through double endonuclease digestion (HindIII-HF and NheI-HF, NEB) followed by agarose gel purification achieved by a combined use of centrifugal filter units (Millipore ...
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bioRxiv - Biochemistry 2024Quote: ... Lec5 and Lec9 CHO cells was extracted using the Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB, T3050L). Resulting gDNA was then sheared by passing each sample 25 times through a 26G needle ...
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bioRxiv - Bioengineering 2024Quote: ... PolyA selection and stranded RNA-seq library preparation were performed with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760; New England Biolabs). Then ...
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bioRxiv - Genomics 2024Quote: ... 1µg RNA was used for first strand cDNA preparation by using ProtoScript II Reverse Transcriptase kit followed by Phusion High-Fidelity DNA Polymerase (NEB). RT-qPCR was performed with LunaScript RT Supermix (NEB ...
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bioRxiv - Zoology 2024Quote: ... The transcriptome sequencing libraries were prepared with poly A selection using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) and were sequenced using the NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Immunology 2024Quote: ... 100 ng of the WTA library was subjected to fragmentation/end-repair/A-tailing using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs). Purified adapter-ligated products were subjected to index PCR ...
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bioRxiv - Genetics 2024Quote: ... after extracting total RNA from the testis (three replicates per genotypes) using the Monarch total RNA mini prep Kit (New England Biolabs), we generated RNA-seq libraries using the NEBNext Rrna Depletion Kit (New England Biolabs ...
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bioRxiv - Synthetic Biology 2024Quote: ... Amplicons were analyzed by 1% agarose gel electrophoresis and purified according to the sizes using the DNA Gel Extraction Kit (NEB).
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bioRxiv - Cancer Biology 2024Quote: ... 1μg RNA was used for sequencing libraries using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to each sample ...
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bioRxiv - Microbiology 2024Quote: High molecular weight DNA for long-read sequencing was extracted using the Monarch HMW DNA extraction kit for tissue (NEB), with the specific protocol for Gram-negative bacteria ...
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bioRxiv - Cancer Biology 2024Quote: ... The purified CASP3 gene fragment was ligated into the linearized pEGFP-N3 vector using the quick ligationTM kit according to the manufacturer’s instructions (NEB, B2200S). The resulting mixture was transformed into competent DH5α bacteria (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Library preparation with indexing was performed by the University of Kansas Genome Sequencing core facility with NEB Next RNA Library kit (NEB). RNAseq was performed using an Illumina NextSeq2000 high-output system with paired-end reads of 50 bp ...
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared from rRNA depleted samples using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) and sequenced on the Novaseq 6000 S4 (Illumina) ...
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bioRxiv - Cell Biology 2024Quote: ... and R211K were introduced into the mKO2-RIβ plasmid using a site-directed mutagenesis kit (New England BioLabs, Ipswich, MA). N-terminal His-tagged human RIβ-WT and RIβ-L50R constructs ...
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bioRxiv - Synthetic Biology 2024Quote: ... where separately introduced to the wild-type MS2 coat protein present in these mRNA constructs using the NEB-Q5 mutagenesis kit (New England Biolabs). The primer sets and PCR settings used are listed in supplementary information ...
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bioRxiv - Neuroscience 2024Quote: ... The remaining steps of library preparation were performed using and the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Adapters and PCR primers were purchased from New England BioLabs ...
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bioRxiv - Molecular Biology 2024Quote: Libraries of immunoprecipitated DNA were generated from 3 ng of starting DNA with the NEBNext Ultra DNA Library Prep kit for Illumina (New England Biolabs) according to manufacturer’s instructions at the CRG Genomics Core Facility (Barcelona ...
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bioRxiv - Synthetic Biology 2024Quote: ... These synthesized fragments were assembled into the Level 0 entry vector pYTK001 using the NEBridge Golden Gate Assembly Kit (BsmBI-v2) (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA from libraries was prepared according to the instructions of the “NEBNext Ultra Directional RNA Library Prep kit for Illumina” (New England Biolabs), following the “Poly(A ...