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Citations for New England Biolabs :
7751 - 7800 of 10000+ citations for Human Complement Component 1 Q Subcomponent Binding Protein HABP1 C1QBP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and 1 μL of 10 mM dNTP mix (NEB N0447S, Ipswich, MA). This initial premix was heated at 65°C for 5 minutes ...
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bioRxiv - Genomics 2023Quote: ... 1 μl 10 mM mix of dGTP and dTTP (NEB #N0442S, #N0443S), 5 μl 10x T4 DNA Ligase Buffer ...
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bioRxiv - Bioengineering 2023Quote: ... the reactions were treated with 1 μl of DpnI (New England BioLabs) for 1 hour at 37 °C before gel purification ...
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bioRxiv - Cell Biology 2023Quote: ... and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1, NEB E7335) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The NEBNext Multiplex Oligos for Illumina (Index Primer Set 1) (NEB, USA) was used for multiplexing ...
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bioRxiv - Cell Biology 2023Quote: ... 3 μM CaCl2) and digested with 1/100 MNase (New England Biolabs). After addition of 250 µL sonication buffer (90 mM Hepes pH 7.9 ...
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bioRxiv - Biophysics 2023Quote: ... 1 μg of the biotinylated lambda DNA is treated with Nt.BspQI (NEB) for 1 hour at 50°C then heat inactivated for 20 minutes at 80°C ...
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bioRxiv - Biophysics 2023Quote: ... and dGTP were incubated and 10 units of DNA polymerase 1 (NEB) for 6 minutes at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... 0.4 µL Antarctic Thermolabile UDG (1 U/µL; New England Biolabs, M0372S), 5.4 µL Bst 2.0 DNA Polymerase (120 U/µL ...
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bioRxiv - Bioengineering 2023Quote: ... the reactions were treated with 1 μl of DpnI (New England BioLabs) for 1 hour at 37 °C before gel purification ...
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bioRxiv - Molecular Biology 2023Quote: Unlabeled purified RNA targets were dephosphorylated using 1 U antarctic phosphatase (NEB) incubated with 1X of provided buffer during 20 min at 37°C and then 5 min at 65°C to inactivate the enzyme ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM EDTA) and passed through an amylose resin column (NEB#E8021L). Columns were washed prior to recombinant protein elution with 10 mM maltose in column buffer.
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bioRxiv - Genomics 2024Quote: ... 1% SDS) and 20 μl of Proteinase K (NEB, cat. no. P8107S). Subsequently ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were grown in 1 mL SOC Outgrowth Medium (B9020, NEB) for 2 h in a standard glass tube at 30 °C with 160 rpm ...
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bioRxiv - Microbiology 2024Quote: Cell lysates were digested for 1 h with Endo H (P0702, NEB) or PNGase F (P0704 ...
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bioRxiv - Microbiology 2024Quote: ... DNA remaining in the RNA samples was removed by DNase 1 (NEB) digestion ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.05% SDS) supplemented with 5.25 µl of 20lJmglJml−1 Proteinase K (NEB) and 1.25 µL of 1lJM DTT (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... for 1 hour at 25°C with 20% PEG8000 (New England Biolabs). After ligation ...
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bioRxiv - Biochemistry 2024Quote: ... either with or without 1 µL of PNGase F (500 Units, NEB).
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bioRxiv - Biochemistry 2024Quote: ... coli exonuclease III (Xth) was performed in NEBuffer 1 (New England Biolabs) with 1 nM enzyme ...
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bioRxiv - Genomics 2024Quote: ... 10 µg of RNA was treated with 1 µL Xrn1 (NEB, M0338S) for 1 h at 37 °C to remove decapped ...
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bioRxiv - Neuroscience 2024Quote: ... with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (NEB). All libraries were sequenced 150bp paired end using an Illumina Novaseq 6000 with 15 million reads on average per sample.
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bioRxiv - Synthetic Biology 2021Quote: ... total DNA from one clone was isolated using the Monarch Kit for HMW DNA Extraction from Bacteria (NEB#T3060) and confirmed by Nanopore sequencing.
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative RT-PCR was performed using Luna Universal One-Step RT-qPCR kit (New England Biolabs, Ipswich, MA, USA) according to manufacturer’s protocol using Agilent Mx3000P instrument ...
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bioRxiv - Cancer Biology 2021Quote: ... Renilla luciferase gene and Firefly luciferase gene were in-vitro transcribed using HiScribe T7 RNA Synthesis Kit (NEB Biolabs). After purified with Monarch RNA Cleanup Kit (NEB Biolabs) ...
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bioRxiv - Cancer Biology 2021Quote: ... Renilla luciferase gene and Firefly luciferase gene were in-vitro transcribed using HiScribe T7 RNA Synthesis Kit (NEB Biolabs). After purified with Monarch RNA Cleanup Kit (NEB Biolabs) ...
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bioRxiv - Cancer Biology 2021Quote: ... before they were used for library construction using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Adaptors were removed from the 3’ end of reads using cutadapt (Martin ...
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bioRxiv - Microbiology 2020Quote: ... RNA was isolated using Invitrogen PureLink RNA Mini Kit following manufacturer’s instructions and treated with DNase (New England Biolabs). Biological triplicate samples were submitted to DNA Technologies and Expression Analysis Core at UC Davis Genome Center for the generation of 3’Tag RNA-Seq libraries ...
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bioRxiv - Microbiology 2020Quote: Site-directed mutagenesis was performed on pBPVP1456 with a Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich, MA) and primers listed in Table 3 ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA-Seq libraries (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, New England BioLabs, Catalog No. E7760) were produced from 16 samples according to the manufacturer’s protocol using 200 ng total RNA ...
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bioRxiv - Cell Biology 2020Quote: RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... addition of a N-terminal Hemagglutinin (HA) epitope was done using Q5 Site-Directed Mutagenesis Kit (New England Biolabs) following manufacturer recommendations.
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bioRxiv - Immunology 2021Quote: ... Devil CIITA was then cloned into SfiI-digested pSBbi-BH using NEBuilder® HiFi DNA Assembly Cloning Kit (NEB). The assembled plasmid pCO2 was transformed into NEB® 5-alpha competent Escherichia coli (High Efficiency ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 RNA levels were quantified using the Luna Universal Probe Onestep RT-qPCR kit (New England Biolabs) and US CDC real-time RT-PCR primer/probe sets for 2019-nCoV_N1 ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB). The sequencing libraries were validated on the Agilent TapeStation (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... and PCR addition of indices (7-11 cycles) was done using NEBNext Ultra II DNA library prep kit (NEB). Biotinylated capture probes 70 nt in length were designed against every DpnII restriction fragment in a 2.5 Mb window centered on Runx1 (chr16:91,566,000-94,101,999) ...
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bioRxiv - Genetics 2021Quote: ... and ligated with the amplicons Uro promoter and GeneSwitch using NEBuilder HiFi DNA Assembly Kit (NEW ENGLAND BioLabs, E2621X). Transgenic lines were generated using standard methods for P-element-mediated germline transformation (BestGene Inc).
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bioRxiv - Genetics 2021Quote: ... The synthetic oligo was cloned into the plasmid backbone by using the Gibson assembly kit (NEB, Gibson Assembly®) and the ligation reaction was carried out for 1 hour at 50 °C ...
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bioRxiv - Genetics 2021Quote: ... High molecular weight gDNA extraction using a Monarch® HMW DNA Extraction Kit for Tissue (New England Biolabs, T3060L) is performed on tissues isolated from N1 or higher generation mice ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were prepared with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645) according to protocol ...
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bioRxiv - Genomics 2020Quote: ... DNA library was prepared using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (New England BioLabs) with 5cycles of pre-amplification and 3 to 7 cycles of PCR amplification ...
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bioRxiv - Molecular Biology 2020Quote: ... and then a cDNA library was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB E7530L). The libraries were checked for quality using the Fragment Analyzer NGS Analysis DNF-474 kit (Advanced Analytical ...
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bioRxiv - Cell Biology 2020Quote: Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
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bioRxiv - Cell Biology 2020Quote: Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
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bioRxiv - Cancer Biology 2020Quote: ... Strand-specific libraries were created using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Hitchin, Herts, UK) and 20ng of the rRNA depleted RNA.
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bioRxiv - Microbiology 2019Quote: ... RNA was extracted with Trizol and ribosomal RNA was removed using a NEBNext rRNA depletion kit (New England BioLabs), according to manufacturer instructions ...
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bioRxiv - Plant Biology 2021Quote: ... was used to generate libraries with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Cat# 7645). The resulting libraries were sequenced (single end 50 bp ...
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bioRxiv - Molecular Biology 2021Quote: ... The library preparation was done using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760S). The libraries were multiplexed (six samples per lane ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA-seq libraries were prepared using Total RNA-seq ScriptSeq Library Prep Kit for Illumina (New England Biolabs) and sequenced using the HiSeq 50 cycle single-end reads protocol on the HiSeq 2500 system ...
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bioRxiv - Evolutionary Biology 2020Quote: ... library preparation was carried out using NEBNext Multiplex Small RNA Library Prep Set for Illumina kit (New England Biolabs) with modified protocol23 for improving low-input samples and sequenced with NextSeq500 (Illumina ...