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Citations for New England Biolabs :
701 - 750 of 1175 citations for Recombinant Mouse IGHG1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... the protein coding sequences were ordered synthetically with Part 3b type BsaI overhangs (NEB R3733) and cloned into the entry vector with BsmBI (NEB R0739) ...
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TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring genebioRxiv - Genetics 2022Quote: ... Chromatin was pre-cleared with 20 μl Protein A magnetic beads (New England Biolabs, S1425S) for 1.5 h and 100 μL of the solution was saved as an input control ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed in 1x NEBuffer for Protein MetalloPhosphatases (New England BioLabs; supplied with λPP), with 0.5% NP-40 and 0.01 mg/mL leupeptin ...
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bioRxiv - Developmental Biology 2023Quote: ... The protein products were purified using the NEBExpress Ni Spin Columns (New England Biolabs, S1427S). The concentration of the purified products was quantified using the Qubit protein assay kit (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were determined via SDS–PAGE and Coomassie staining using BSA (New England Biolabs) as a standard ...
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bioRxiv - Molecular Biology 2023Quote: RNA-protein complexes were isolated with oligo[dT)]25 magnetic beads (New England Biolabs, S1419S). Briefly ...
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bioRxiv - Immunology 2023Quote: ... For embryo microinjection the annealed sgRNA were each complexed with Cas9 protein (New England Biolabs) at room temperature for 10min in injection buffer (final concentrations ...
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bioRxiv - Biophysics 2023Quote: ... MBP-HNRNPH2 proteins were purified by affinity chromatography using amylose resin (#E8021, New England BioLabs) and eluted with buffer containing 20 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... the immunity protein genes were amplified by PCR using Phusion polymerase (New England Biolabs, UK) with the following primers ...
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bioRxiv - Bioengineering 2023Quote: ... The luciferase protein expressed in NiCo21(DE3) competent Escherichia coli (New England Biolabs, MA, USA) was purified using a Ni-NTA agarose resin column (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... All aliquots were supplemented with 10X NEBuffer for Protein MetalloPhosphatases (PMP) and 10X MnCl2 (NEB). The first aliquot (Untreated control ...
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bioRxiv - Physiology 2024Quote: ... 20 mg of protein from each sample was subjected to PNGase F treatment (NEB #P0704) to remove N-linked oligosaccharides prior to SDS-PAGE ...
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bioRxiv - Neuroscience 2024Quote: ... 30 µl of protein A or G paramagnetic beads (73778S or 9006S; New England Biolabs) were washed and then complexed with primary antibodies or naïve rabbit IgG at 4°C with end-over-end rotation ...
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bioRxiv - Biochemistry 2023Quote: ... protein was combined with 1.5x molar excess of SNAP-Surface Alexa488 dye (NEB, Cat# S9129S). SNAP dye labeling was performed in buffer containing 20 mM Tris [pH 8.0] ...
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bioRxiv - Biochemistry 2024Quote: ... Soluble protein fractions were mixed with an amylose resin column (New England BioLabs, Beverly, MA) pre-equilibrated with buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... Initial protein-level deglycosylation was performed using 250 U of Endo H (New England Biolabs) for 5 µg trimer ...
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bioRxiv - Developmental Biology 2024Quote: ... adrb1-/- zebrafish was obtained by coninjection of Cas9 protein (EnGen Spy Cas9 NLS, M0646, NEB) with gRNA targeting adrb1 ...
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bioRxiv - Cell Biology 2024Quote: ... 100 µg cell lysate was mixed with 3,200 U λ protein phosphatase (New England Biolabs) and incubated at 37 °C for 1 h to achieve complete protein dephosphorylation ...
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bioRxiv - Biochemistry 2024Quote: All protein mutagenesis was performed with the Q5 site-directed mutagenesis kit from NEB (E0554). Primers were designed with the NEBaseChanger tool (https://nebasechanger.neb.com/ ...
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bioRxiv - Biochemistry 2024Quote: In Vitro Translation (IVT) was performed using the PURExpress in vitro protein synthesis kit (NEB). Each IVT reaction contained 4 μL of PURE Solution A ...
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bioRxiv - Microbiology 2024Quote: ... The in vitro translation assay was performed using PURExpress In Vitro Protein Synthesis Kit (NEB). 24 μM of antibiotics were added individually to the translation mixture and incubated at 37 °C for 4 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... depleted with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat, NEB, Figures 6 and S6), or not depleted (Figures 2B ...
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bioRxiv - Genomics 2022Quote: For each of the two ligation-based protocols used for mouse sperm (Truseq, NEB Next), two replicate libraries for mouse sperm were prepared with the additional condition of an 18 hour ligation at 16°C for the ligation of the 3’ adapter in the attempt to increase ligation efficiency ...
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bioRxiv - Developmental Biology 2022Quote: ... total RNA (1 µg) extracted from adult mouse testes was treated with alkaline phosphatase (NEB), de-phosphorylated ...
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bioRxiv - Cancer Biology 2023Quote: ... Ribosomal RNA was removed using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat; NEB, E6310X). rRNA-depleted RNA was converted to a library using NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Cell Biology 2020Quote: Purified GST-Bbs5 protein (400 μg) was incubated with 6 μl of PKC (New England Biolabs) and 10 μl of ATP (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins on glutathione beads were pre-treated with 250 units CK2 (New England Biolabs) in protein kinase buffer (New England Biolabs ...
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bioRxiv - Developmental Biology 2021Quote: ... and the protease enzyme was further removed from hTNPO1 protein by Ni-NTA Magnetic Beads (NEB) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Precipitated proteins were wash once in acetone and resuspended in 1X blue loading buffer (NEB, B7703S).
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bioRxiv - Molecular Biology 2021Quote: ... The resulting PCR product was ligated into the pMal-c2x protein expression plasmid (New England Biolabs) adjacent to the malE gene to generate the pMAL-c2x-malE-L375-his10 plasmid encoding maltose binding protein (MBP ...
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bioRxiv - Developmental Biology 2021Quote: ... The guide RNA was injected into one-cell stage embryos together with Cas9 protein (N.E. Biolabs). Mutant animals were identified by PCR using the following primers ...
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bioRxiv - Biochemistry 2019Quote: Assays were conducted by incubating 0.1 and 1 μM SidJ in 1X Protein Kinase buffer (NEB), with 10 mM CaCl2 ...
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bioRxiv - Bioengineering 2021Quote: ... Amplified protein and pCTCON backbone were digested with BamHI and NheI restriction enzymes (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5 μM protein was incubated in the presence or absence of 20 μM SAM (NEB #B9003) in 25 mM HEPES pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... Surface biotinylated proteins immobilized on streptavidin agarose beads were denatured in Denaturing Buffer (New England Biolabs) at 100°C for 10 min in accordance with the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... All proteins were overexpressed in Escherichia coli BL21 Codon-Plus competent cells (C2527, New England Biolabs) and grown at 37 °C in 2 % Luria Broth containing 30 mg/L kanamycin until OD600 reached 0.6 ...
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bioRxiv - Microbiology 2021Quote: One microgram of full-length S protein was treated with 1 μg of FXa (P8010L, NEB), furin (P8077S ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM MnCl2 only or together with 1 μl Lambda Protein Phosphatase (New England Biolabs). After incubation at 30°C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... MBP-tagged protein was captured by gravity flow affinity chromatography using amylose resin (New England Biolabs). Captured protein was washed with wash buffer (50mM Tris/HCl pH 7.6 ...
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bioRxiv - Biochemistry 2022Quote: ... The fusion protein was purified from the supernatant using amylose resin (New England Biolabs, Ipswich, MA), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... together with the Cas9 protein (EnGen® Cas9 NLS, S. pyogenes, M0646M, NEB, Ipswich, MA, USA) was injected into zebrafish embryos at one-cell stage ...
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bioRxiv - Molecular Biology 2020Quote: ... half of the samples were treated with a broad-spectrum lambda protein phosphatase (NEB Cat#P0753L) and PNGase-F (Promega Cat#V4831 ...
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bioRxiv - Bioengineering 2021Quote: 25 µl reactions were set up following the PURExpress protocol (In Vitro Protein Synthesis Kit, NEB) in black low-volume 384-well polystyrene plates (Corning) ...
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bioRxiv - Microbiology 2019Quote: Cellular extracts were incubated with 2,000 units of λ-protein phosphatase (λ-Ppase) (New England Biolabs) in 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein-RNA complexes were radiolabeled on-bead in 40 µl reactions with T4 polynucleotide kinase (NEB) and 2 µl [γ-32P]ATP (6,000 Ci/mmol ...
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bioRxiv - Genomics 2019Quote: Cas9/RNP complexes were assembled with 25 pmol Cas9 protein (EnGen Cas9 NLS, New England Biolabs) and 50 pmol gRNA just prior to electroporation ...
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bioRxiv - Cell Biology 2019Quote: ... SNAP-FOR1(3P,FH2) protein was labeled with SNAP-549 dye (New England Biolabs, Ipswich, MA) as per manufacturer’s instructions prior to each TIRF experiment.
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bioRxiv - Cell Biology 2020Quote: Total protein extracts were obtained from cell pellets directly resuspended in the loading buffer (Biolabs B7709S) containing 1% SDS ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were prediluted to a concentration of 750 nM in 1x CutSmart buffer (New England Biolabs) and preloaded with guide DNA or guide RNA in a 1:1 ratio at 37°C for 15 min ...
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bioRxiv - Neuroscience 2020Quote: ... All proteins were overexpressed in Escherichia coli BL21 Codon-Plus competent cells (C2527, New England Biolabs) and grown at 37 °C in 2 % Luria Broth containing 30 mg/L kanamycin until OD600 reached 0.6 ...