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Citations for New England Biolabs :
701 - 750 of 1480 citations for Guanine Nucleotide Binding Protein G Protein Alpha Activating Activity Polypeptide O GNAO1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The lambda protein phosphatase procedure was carried out following the manufacturer’s guidelines (New England Biolabs). The sample was incubated at 30°C for 2 hours ...
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bioRxiv - Biophysics 2022Quote: Plasmids expressing stdMCP-fusion proteins were generated using the NEBuilder HiFi DNA Assembly kit (NEB). To obtain plasmids carrying an stdGFP-tagged stdMCP gene ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein coding sequences were ordered synthetically with Part 3b type BsaI overhangs (NEB R3733) and cloned into the entry vector with BsmBI (NEB R0739) ...
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TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring genebioRxiv - Genetics 2022Quote: ... Chromatin was pre-cleared with 20 μl Protein A magnetic beads (New England Biolabs, S1425S) for 1.5 h and 100 μL of the solution was saved as an input control ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed in 1x NEBuffer for Protein MetalloPhosphatases (New England BioLabs; supplied with λPP), with 0.5% NP-40 and 0.01 mg/mL leupeptin ...
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bioRxiv - Developmental Biology 2023Quote: ... The protein products were purified using the NEBExpress Ni Spin Columns (New England Biolabs, S1427S). The concentration of the purified products was quantified using the Qubit protein assay kit (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were determined via SDS–PAGE and Coomassie staining using BSA (New England Biolabs) as a standard ...
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bioRxiv - Molecular Biology 2023Quote: RNA-protein complexes were isolated with oligo[dT)]25 magnetic beads (New England Biolabs, S1419S). Briefly ...
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bioRxiv - Immunology 2023Quote: ... For embryo microinjection the annealed sgRNA were each complexed with Cas9 protein (New England Biolabs) at room temperature for 10min in injection buffer (final concentrations ...
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bioRxiv - Biophysics 2023Quote: ... MBP-HNRNPH2 proteins were purified by affinity chromatography using amylose resin (#E8021, New England BioLabs) and eluted with buffer containing 20 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... the immunity protein genes were amplified by PCR using Phusion polymerase (New England Biolabs, UK) with the following primers ...
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bioRxiv - Bioengineering 2023Quote: ... The luciferase protein expressed in NiCo21(DE3) competent Escherichia coli (New England Biolabs, MA, USA) was purified using a Ni-NTA agarose resin column (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... All aliquots were supplemented with 10X NEBuffer for Protein MetalloPhosphatases (PMP) and 10X MnCl2 (NEB). The first aliquot (Untreated control ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg cell lysate was mixed with 3,200 U λ protein phosphatase (New England Biolabs) and incubated at 37 °C for 1 h to achieve complete protein dephosphorylation ...
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bioRxiv - Physiology 2024Quote: ... 20 mg of protein from each sample was subjected to PNGase F treatment (NEB #P0704) to remove N-linked oligosaccharides prior to SDS-PAGE ...
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bioRxiv - Biochemistry 2023Quote: ... protein was combined with 1.5x molar excess of SNAP-Surface Alexa488 dye (NEB, Cat# S9129S). SNAP dye labeling was performed in buffer containing 20 mM Tris [pH 8.0] ...
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bioRxiv - Biochemistry 2024Quote: ... Soluble protein fractions were mixed with an amylose resin column (New England BioLabs, Beverly, MA) pre-equilibrated with buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... Initial protein-level deglycosylation was performed using 250 U of Endo H (New England Biolabs) for 5 µg trimer ...
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bioRxiv - Biochemistry 2024Quote: ... PURExpress-derived protein was mixed in a reaction containing 1x NEBuffer 2.1 (New England Biolabs), three-fold diluted PURExpress-derived protein ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was further purified on a 5-10 mL amylose column (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Bioengineering 2023Quote: The heterologous protein MBPeGFP was purified using NEBExpress Ni spin columns (NEB, Ipswich, MA, USA) according to manufacturer recommendations ...
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bioRxiv - Immunology 2019Quote: ... PCR product was cloned into 5-alpha competent bacteria (New England Biolabs, cat. #C2987) using a TOPO TA cloning kit (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2022Quote: Escherichia coli DNA adenine methyltransferase (dam)+/dcm+ (NEB 5-alpha competent E. coli, #C2987) and dam−/dcm− (dam−/dcm− competent E ...
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bioRxiv - Microbiology 2021Quote: ... coli transformations were performed using NEB 5-alpha chemically competent cells (New England BioLabs). E ...
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bioRxiv - Microbiology 2023Quote: ... All the produced constructs were propagated in competent cells (5-alpha Competent E.coli; NEB) and isolated by NucleoSpin Plasmid (TaKaRa) ...
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bioRxiv - Genomics 2024Quote: ... Ligation products were transformed into either 5-alpha or 10-beta electrocompetent cells (NEB) and grown in liquid LB-Amp cultures ...
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bioRxiv - Biochemistry 2022Quote: ... The RNA was first hydrolyzed overnight to nucleotide monophosphates using 300 U/ μg Nuclease P1 (NEB, 100,000 U/mL) at 37°C in 100 mM ammonium acetate (pH 5.5 ...
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bioRxiv - Biochemistry 2020Quote: ... 14 nucleotides in length (TIBMOL BIOL, Berlin) were radioactively phosphorylated with [32γP] dATP at their 5’ ends using phosphokinase (NEB) following the manufacturer protocol and used as a length standards.
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bioRxiv - Pathology 2021Quote: ... Single nucleotide mutations (SNMs) in tcdB were generated using Q5 Site-Directed Mutagenesis according to manufacturer’s protocol (New England Biolabs). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Oligonucleotide probe for telomere or Alu repeats was labeled with [32P]-ATP (3,000 Ci/mmol) and T4 nucleotide kinase (New England Biolabs). The membrane was hybridized in Church hybridization buffer containing a 32P-labeled probe at 42°C overnight ...
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bioRxiv - Genetics 2023Quote: ... which was immediately used as a template for seven synonymous single-nucleotide substitutions into the terminal 21bp of the ZK938.12 gene fragment by Q5 site-directed mutagenesis kit (NEB). The resultant plasmid was named pDSP47.
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bioRxiv - Microbiology 2021Quote: ... Plasmids from O/N cultures were extracted with the Monarch Plasmid Miniprep Kit (New England Biolabs) and sent for sequencing to LGC genomics.
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bioRxiv - Biochemistry 2021Quote: Native glycoproteins (10 μg) were digested with 1 U of IMPa (O-Glycoprotease, New England Biolabs) in 50 μL 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... the synthetic O-glycopeptide was incubated with 200 U of α2-3,6,8 Neuraminidase (New England Biolabs) for 16 h at 37°C ...
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bioRxiv - Genomics 2022Quote: ... w/o any promoter for promoter-assays were first digested using EcoRV (New England Biolabs, R3195S). Next ...
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bioRxiv - Physiology 2022Quote: ... O-GlcNAc site were mutated into alanine with Q5® Site-Directed Mutagenesis Kit (NEB#E0554). Lentivirus were packed as previously described (Huang et al. ...
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bioRxiv - Cell Biology 2020Quote: Purified GST-Bbs5 protein (400 μg) was incubated with 6 μl of PKC (New England Biolabs) and 10 μl of ATP (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins on glutathione beads were pre-treated with 250 units CK2 (New England Biolabs) in protein kinase buffer (New England Biolabs ...
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bioRxiv - Developmental Biology 2021Quote: ... and the protease enzyme was further removed from hTNPO1 protein by Ni-NTA Magnetic Beads (NEB) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Precipitated proteins were wash once in acetone and resuspended in 1X blue loading buffer (NEB, B7703S).
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bioRxiv - Molecular Biology 2021Quote: ... The resulting PCR product was ligated into the pMal-c2x protein expression plasmid (New England Biolabs) adjacent to the malE gene to generate the pMAL-c2x-malE-L375-his10 plasmid encoding maltose binding protein (MBP ...
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bioRxiv - Developmental Biology 2021Quote: ... The guide RNA was injected into one-cell stage embryos together with Cas9 protein (N.E. Biolabs). Mutant animals were identified by PCR using the following primers ...
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bioRxiv - Biochemistry 2019Quote: Assays were conducted by incubating 0.1 and 1 μM SidJ in 1X Protein Kinase buffer (NEB), with 10 mM CaCl2 ...
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bioRxiv - Bioengineering 2021Quote: ... Amplified protein and pCTCON backbone were digested with BamHI and NheI restriction enzymes (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5 μM protein was incubated in the presence or absence of 20 μM SAM (NEB #B9003) in 25 mM HEPES pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... Surface biotinylated proteins immobilized on streptavidin agarose beads were denatured in Denaturing Buffer (New England Biolabs) at 100°C for 10 min in accordance with the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... All proteins were overexpressed in Escherichia coli BL21 Codon-Plus competent cells (C2527, New England Biolabs) and grown at 37 °C in 2 % Luria Broth containing 30 mg/L kanamycin until OD600 reached 0.6 ...
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bioRxiv - Microbiology 2021Quote: One microgram of full-length S protein was treated with 1 μg of FXa (P8010L, NEB), furin (P8077S ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM MnCl2 only or together with 1 μl Lambda Protein Phosphatase (New England Biolabs). After incubation at 30°C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... MBP-tagged protein was captured by gravity flow affinity chromatography using amylose resin (New England Biolabs). Captured protein was washed with wash buffer (50mM Tris/HCl pH 7.6 ...