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Citations for New England Biolabs :
7401 - 7450 of 10000+ citations for Mouse Dachshund Homolog 1 DACH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs). sPOINT was performed in the same manner with the following difference ...
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bioRxiv - Biochemistry 2024Quote: ... a vector containing an N-terminal Thioredoxin-His10 tag using a Gibson Assembly® Cloning Kit (New England BioLabs). Plasmids were extracted and transformed into competent E ...
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bioRxiv - Molecular Biology 2024Quote: ... Colonies were selected and grown overnight in LB to extract plasmid DNA using a MiniPrep kit (NEB, Massachusets, USA) using manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and PCR enrichment were performed sequentially using the NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB). Finally ...
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bioRxiv - Neuroscience 2024Quote: ... and multiplexed RNA-seq libraries were generated using the NEBNext Ultra Directional RNA library Prep Kit for Illumina (NEB). Libraries were sequenced (75 bp ...
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bioRxiv - Neuroscience 2024Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 100ng input RNA was used for library preparation with the NEBNext Globin & rRNA depletion kit (New England Biolabs, E7750X) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... The cDNA for catalytically inactive E447D mutant was generated through mutation of StcEΔ35 using the Q5 Site-Directed Mutagenesis Kit (Cat # E0552S, New England Biolabs) with primers 5’-TCAGTCATGACGTTGGTCATAATTATG-3’ and 5’-ACTCATTCCCCAATGTGG-3’ ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragmentation was performed using the NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs), an enzymatic fragmentation assay with an average fragment size of 380 bp ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then treated with DNase I and cleaned with the Monarch RNA Cleanup Kit (NEB, Ipswich, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... EM-seq was performed with 100ng of purified genomic DNA using NEBNext® Enzymatic Methyl-seq Kit (NEB, E7120) and following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Digested oligos and vector were gel-purified with Qiagen gel purification kit and ligated by T4 DNA ligase (NEB). Ligate was transformed into Stbl3 competent bacteria (Thermo Fisher Scientific) ...
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Fluoroquinolone-Specific Resistance Trajectories in E. coli and their Dependence on the SOS-ResponsebioRxiv - Microbiology 2024Quote: ... Genomic DNA libraries were created according to the manufacturer’s protocol using NEBNext Ultra II FS DNA Library Prep kit for Illumina (New England BioLabs) in combination with NEBNext multiplex oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Genetics 2024Quote: ... ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) without size selection ...
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bioRxiv - Molecular Biology 2024Quote: ... libraries were prepared for all samples using the NEB Next Ultra II Directional RNA Library Prep Kit (NEB, E7760S) according to manufacturer protocol ...
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bioRxiv - Immunology 2024Quote: Site-directed mutants in this study were generated using a Q5 site directed mutagenesis kit according to the manufacturer’s instructions (NEB). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: 50 ng of DNA template was transcribed using a HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s protocol but at one-quarter volume (5 µl) ...
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bioRxiv - Microbiology 2024Quote: ... part of the ONT Kit) to the RNA sample using a mix of 3μl NEBNext Quick Ligation Reaction Buffer (New England BioLabs), 0.5μl RNA CS (ONT Kit) ...
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bioRxiv - Microbiology 2024Quote: ... RIG-I and ISG15 were determined using Luna Universal One-Step RT-qPCR Kit according to the manufacturer’s instructions (New England Biolabs). The primer sequences are available upon request ...
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bioRxiv - Neuroscience 2024Quote: The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... The purified DNA samples were then used for library preparation using NEBNext UltraII DNA Library prep kit (NEB, E7370L) and were sequenced at the iGE3 platform of the University of Geneva.
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation was carried out on the purified DNA using NEBNext Ultra II DNA Library Prep Kit (NEB #E7645) and sequenced at 100 million reads per sample on a NovaSeq6000 using 75-nucleotide read length paired-end sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation was done using NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (NEB #E7645, #E7103). DNA was sequenced on Illumina HiSeq 2500 platform.
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bioRxiv - Molecular Biology 2024Quote: ChIP-seq libraries were built using an NEBNext® Ultra™ II DNA Library Prep Kit (NEB #E7645S/L) with immunoprecipitated or input DNA ...
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bioRxiv - Developmental Biology 2024Quote: ... The DNA-Seq libraries were prepared using the NEBNext DNA library preparation kit according to the manufacturer’s instructions (E6040, New England Biolabs) and the libraries were sequenced on an Illumina HiSeq 4000 instrument ...
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bioRxiv - Synthetic Biology 2024Quote: All transcription templates were generated using PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs, catalog no. E0553) of an oligo that includes a T7 RNAP promoter ...
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bioRxiv - Pathology 2024Quote: ... Library preparation was completed on ribo-depleted RNA using the NEBNext Ultra Directional RNA Library prep kit (NEB, #E7420L) using 8 cycles of PCR amplification and indexed using single (i7 ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified with DNA Spin and Concentrate kit (Zymo Research, Irvine, CA, D4013 or NEB Monarch, T1030) following manufacturer instructions or gel-purified from kit (Zymo Research) ...
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bioRxiv - Genomics 2024Quote: ... RNA sequencing libraries were prepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB) according to the recommended protocol using 1 ng RNA as input material ...
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bioRxiv - Genomics 2024Quote: ... RNA-seq libraries were constructed using the NEBNext® Ultra™ RNA Library Prep Kit (NEB, Ipswich, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... IVT was performed on the purified PCR product using a HiScribe T7 High yield RNA Synthesis Kit (NEB, E2040S) and purified using a Monarch RNA Cleanup Kit (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Mutations M1 (CAGGTGcAac) and M2 (CAGGTGTACA) in pX_Luc were introduced using Q5 site-directed mutagenesis kit (New England Biolabs). Plasmids expressing DYRK1A or DYRK1A_KR under the control of the CMV promoter were previously described [29].
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bioRxiv - Developmental Biology 2024Quote: ... Deletions of these constructs were constructed using the Q5 site-directed mutagenesis kit according to the manufacturer protocol (NEB). For construction of pMT-ed:Flag:vhh4 ...
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bioRxiv - Molecular Biology 2024Quote: ... All qPCRs were performed using the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA) and a Step One Plus qPCR machine (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from bulk liver tissue using the Monarch total RNA miniprep kit (New England Biolabs, Ipswich, MA) after homogenization with steel beads using a TissueLyser LT bead mill (Qiagen ...
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bioRxiv - Genomics 2024Quote: ... Small RNA libraries were generated using the NEBnext small RNA library preparation kit according to instructions (New England BioLabs). small RNA high-throughput sequencing libraries were sequenced for 101 cycles using NovaSeq X Plus ...
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bioRxiv - Synthetic Biology 2024Quote: ... All connections between genes and linearized vectors were achieved by Gibson assembly (Gibson assembly kit, New England BioLabs, UK). The shell-(csoS2-NM) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Lettuce variant sequences were cloned into retron Eco2 plasmid using Q5® Site-Directed Mutagenesis Kit (New England Biolabs). Primer sequences and annealing temperatures were generated using the NEBaseChanger™ tool.
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bioRxiv - Synthetic Biology 2024Quote: ... RNA strands were transcribed in-house and column-purified using the Monarch® RNA Cleanup Kit (New England Biolabs). The concentration of oligonucleotides was quantified by UV-vis spectrophotometry using a Thermo Fisher Scientific NanoDrop 2000 Spectrophotometer ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the amplified plasmid DNA was extracted using the Monarch® DNA Gel Extraction Kit (New England Biolabs, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was extracted using the Monarch HMW DNA Extraction Kit for Tissues (New England Biolabs, Ipswitch, MA, USA). Long-read sequencing was performed on the GridION platform (Oxford Nanopore Technologies ...
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bioRxiv - Microbiology 2024Quote: ... illumina sequencing libraries were prepared with a NEBNext®Ultra™DNA Library preparation kit (New England Biolabs, USA) of 500 mg DNA samples from each sample ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of total RNA was utilized to construct the sequencing libraries using NEBNext Ultra RNA Library Prep Kit for Illumina as per the manufacturer’s protocol (NEB) and paired-end sequencing was performed with NovaSeq 6000 platform (Illumina).
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of DNA template was transcribed using a HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s protocol for 11 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... To ensure this we performed the Oligonucleotide Clean-up protocol of the Monarch PCR & DNA Clean-up kit (NEB) on 10 PCR reaction tubes ...
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bioRxiv - Microbiology 2024Quote: ... cloned into the pre-assembled vector using the NEBridge® Golden Gate Assembly Kit (BsaI-HF® v2; NEB). A synonymous point mutation (A17973G ...
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bioRxiv - Microbiology 2024Quote: ... NGS libraries were prepared by NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB, MA, USA) following manufacturer instructions and sequencing was conducted by NovaSeq 6000 (PE150 ...
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bioRxiv - Synthetic Biology 2024Quote: All plasmid E.coli plasmid DNA minipreps were performed using the NEB Monarch Plasmid Miniprep Kit and Protocol (NEB #T1010). Prior to starting the miniprep protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) (NEB: E7335L). Samples with total RNA concentration of >10 ng/µl (0.5 µg total amount ...
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bioRxiv - Genetics 2021Quote: ... then a final 1 min extension (72 °C)—using Phusion Flash polymerase (NEB) from cDNA clones using primers containing an EagI restriction site (forward primers ...