Labshake search
Citations for New England Biolabs :
7301 - 7350 of 9360 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... Reverse transcription and quantitative real-time PCR was performed using Luna Universal One-Step RT-qPCR Kit (New England Biolabs) in a 96-well plate and qPCR was performed in a LightCycler 96 System (Roche) ...
-
bioRxiv - Developmental Biology 2023Quote: The donor plasmid pBS-Eno-Mutpi was generated by assembly of PCR fragments into pBS-SK+ digested by EcoRI and KpnI using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB) and primers designed for amplifying ≈1 kb long homology arms from w1118 genomic sequence ...
-
bioRxiv - Genetics 2023Quote: ... Single-stranded RNA was synthesized from the PCR product using Hiscribe T7 Quick High Yield RNA Synthesis Kit (E2050S, NEB). The ssRNA was then converted into ssDNA using Maxima H Minus Reverse Transcriptase (EP0753 ...
-
bioRxiv - Biophysics 2023Quote: ... by site-directed mutagenesis of the HT-rad21 and HT-CTCF plasmids using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs) according to the manufacturer’s protocol with primers given in Supplementary Table 18 ...
-
bioRxiv - Bioengineering 2023Quote: ... The IVT reaction product was treated with DNase I to remove the DNA template and then purified using the Monarch RNA clean-up kit (NEB). dgRNA concentration was measured using a NanoDrop.
-
bioRxiv - Cell Biology 2023Quote: ... FLAG-tagged transgenes of AMOTL2 and HA-tagged MAGI-1 WW domains were obtained from Integrated DNA Technologies as gBlock HiFi Gene Fragments and cloned into the pCMV6 backbone via Gibson assembly using a HiFi DNA Assembly Cloning Kit (NEB).
-
bioRxiv - Biochemistry 2023Quote: ... mRNA was transcribed from 500 ng purified linearized template using the HiScribe T7 High-Yield RNA Synthesis Kit (New England BioLabs) with co-transcriptional capping by CleanCap AG (TriLink Biotechnologies ...
-
bioRxiv - Biochemistry 2023Quote: ... and linearized by the appropriate Type IIS enzyme (BspQI or BsmBI) and purified by Monarch RNA Cleanup kit (New England Biolabs) prior to IVT reactions.
-
bioRxiv - Systems Biology 2023Quote: ... RT-qPCR quantification was carried out on 20 ng of RNA with the Luna One-Step RT-qPCR Kit (NEB). All qPCRs were performed in technical triplicates ...
-
bioRxiv - Biochemistry 2023Quote: ... was used in sequential reverse transcription and PCR amplification steps using the Protoscript II first strand cDNA synthesis kit and Q5 Hi-fidelity DNA polymerase (NEB) according to manufacturer’s protocols ...
-
bioRxiv - Cancer Biology 2023Quote: ... Sequence ready polyA-enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina (NEB, E7760), according to manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2023Quote: ... brucei BFs CN PIP5Pase exclusively expressing V5-tagged PIP5Pase WT or mutant D360A/N362A for 24 h using the magnetic mRNA isolation kit (New England Biolabs) according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: Quantitative real-time PCR (qRT-PCR) was used to measure RNA with Luna Universal One-Step RT-qPCR Kit (New England Biolabs) via CFX Connect Real-Time PCR Detection System (Bio-Rad) ...
-
bioRxiv - Microbiology 2023Quote: Genes of interest were PCR-amplified with 25 bp homology arms from high-titer phage lysates and ligated into respective plasmid backbones using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). Recombinant plasmids were transformed into E ...
-
bioRxiv - Microbiology 2023Quote: ... PolyA+ RNA was purified from ∼100 ng of total RNA and sequencing libraries were prepared with the NEBNext Ultra II RNA library kit (New England Biolabs) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... The fragments containing the transposon-junctions were amplified by PCR and prepared for sequencing using the NEB Ultra I kit (New England Biolabs) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... and RNA-seq libraries were prepared using a NEB-Next Ultra II Directional Library Prep Kit for Illumina (E7760, NEB). Library sizes were determined on a Bioanalyzer ...
-
bioRxiv - Genomics 2023Quote: ... Library preparation was performed using the NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs Inc. MA, USA) for strand-specific Illumina libraries ...
-
bioRxiv - Genomics 2023Quote: ... A transcription reaction was then carried out on the purified and quantified dsDNA using the T7 HiScribe transcription kit (NEB). The T7 RNA Polymerase recognizes the T7 promoter region that seeds transcription of the adjacent 20-mer target sequence thus generating the targeting sgRNA for Cas9-mediated nicking of genomic DNA templates ...
-
bioRxiv - Microbiology 2024Quote: ... equal volumes of cDNA were used for qPCR using the Luna® Universal qPCR Master Mix kit (New England Biolabs). For qPCR ...
-
bioRxiv - Immunology 2024Quote: ... 10ng RNA was used to prepare libraries using Single Cell/Low Input RNA Library Prep Kit for Illumina (New England BioLabs). Libraries with different indexes were pooled and sequenced by an Illumina NovaSeq 6000 as paired-end reads extending 150 bases.
-
bioRxiv - Physiology 2024Quote: ... The libraries for the Illumina sequencer (paired-end 150 bp) were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2024Quote: ... Around 5 ng of purified CUT&RUN-enriched DNA was used to prepare NGS libraries using the NEBNext Ultra II DNA Library Prep Kit (NEB) with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1 ...
-
bioRxiv - Cancer Biology 2024Quote: ... the NEBNext Ultra II directional RNA library prep kit with the NEBNext Poly(A) mRNA magnetic isolation module (New England Biolabs) was utilized according to the manufacturer’s protocol ...
-
bioRxiv - Genetics 2024Quote: ... Cas9H840A was made by site-directed mutagenesis of pLS504 using the Q5 site-directed mutagenesis kit (New England Biolabs #E0554S) using primers MK118 and MK119 to yield pLS517 ...
-
bioRxiv - Cell Biology 2024Quote: ... was inserted into murine Shh between amino acids 92N and 93T (corresponding to N91 and T92 in human Shh) by using Gibson assembly (HiFi Assembly Kit, NEB). Where indicated ...
-
bioRxiv - Cancer Biology 2024Quote: ... range 9.0-9.5] were converted into sequencing libraries with the “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” (#E7760, New England Biolabs) following the manufacturer’s instructions ...
-
bioRxiv - Physiology 2024Quote: ... Five hundred micrograms of total RNA was used to prepare strand-specific libraries by the NEBNext Ultra II Directional RNA Library Preparation kit (NEB) and sequenced (75 nucleotides ...
-
bioRxiv - Genomics 2024Quote: ... PCR amplified and then pooled into equimolar amounts and further quantified using the NEBNext Library Quant Kit (New England Biolabs). The pooled library was subjected to 50 bp paired-end sequencing on the Illumina NextSeq 2000 platform.
-
bioRxiv - Immunology 2024Quote: ... Stranded RNA sequencing libraries were prepared as described using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (Cat# E7760L; New England Biolabs). Purified libraries were qualified on an 4150 TapeStation (Agilent Technologies ...
-
bioRxiv - Microbiology 2024Quote: ... Libraries were prepared using NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (New England Biolabs; #E6177) per manufacturers protocol with 50-100 ng of DNA input and 5-6 cycles of PCR ...
-
bioRxiv - Cell Biology 2024Quote: ... To generate pcDNA5/FRT-Tg-NLuc plasmids the Tg gene was amplified from the pcDNA5/FRT-Tg-FLAG plasmid and assembled with the NLuc fragment using a HiFi DNA assembly kit (New England BioLabs). To generate the respective mutant construct plasmids for A2234D and C1264R Tg ...
-
bioRxiv - Cell Biology 2024Quote: Mutations in RAD54L were generated in pENTR1A-RAD54L-HA (31) using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) (Table S4) ...
-
bioRxiv - Neuroscience 2024Quote: ... RNA input was normalized and NGS libraries were prepared using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England BioLabs) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... Overhanging BsWI sites were added to the oligonucleotides for cloning into the dCasRx plasmid with Quick Ligation kit (M2200S, New England Biolabs) (FW ...
-
bioRxiv - Molecular Biology 2024Quote: ... 3µl of annealed oligonucleotides were cloned in 50ng of gRNA backbone vector (previously cut with BbsI-HF and gel extracted) using Quick Ligation kit (#M2200S, New England Biolabs) and transformed in NEB 5-alpha Competent E ...
-
bioRxiv - Cell Biology 2024Quote: ... Total RNA obtained from each sample was subjected to sequencing library construction using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) with NEBNext Poly(A ...
-
bioRxiv - Cancer Biology 2024Quote: mRNA was isolated from 6-well plates showing a cell confluency of ∼90% using the Monarch Total RNA Miniprep kit (NEB). siRNA transfection was performed 48 hours prior to RNA isolation using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... and processed into a cDNA library for Illumina with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Dual-index primers were conjugated with NEBNext Multiplex Oligos for Illumina (Set1 ...
-
bioRxiv - Microbiology 2024Quote: ... Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversely transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Samples were polyA-enriched and directional libraries were prepared using the NEBNext Ultra II Directional Library Prep Kit for Illumina (NEB). Libraries were PE150 sequenced on a NovaSeq 6000 (Illumina ...
-
bioRxiv - Cell Biology 2024Quote: ... Library preparation was carried out by the Edinburgh Clinical Research Facility from 500 ng of each RNA sample using the NEBNext Ultra II Directional RNA library kit with PolyA enrichment module (New England Biolabs). Libraries were assessed for quality and fragment size using the Agilent Bioanalyser ...
-
bioRxiv - Cell Biology 2024Quote: ... The vector backbone was also PCR-amplified and the three fragments were assembled with NEBuilder HiFi DNA assembly kit (New England Biolabs) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... Up to 1ug of RNA is then converted into cDNA using the NEB LunaScript® RT Supermix Kit (NEB #E3010) followed by qPCR using the NEB Luna Universal qPCR Master Mix (NEB #M3003) ...
-
bioRxiv - Cancer Biology 2024Quote: ... RNA of 2×106 cells was extracted with the Monarch Total RNA Miniprep Kit with “on column” DNase I treatment (NEB). cDNA was generated by using LunaScript RT SuperMix Kit (NEB ...
-
bioRxiv - Molecular Biology 2024Quote: ... The preparation of sequencing libraries for ribosome profiling was conducted via the NEBNext Multiplex Small RNA Library Prep kit for Illumina according to the manufacturer’s protocol (NEB, #E7300S). Pair-end sequencing reads of size 150 bp were produced for Ribo-seq on the Ilumina Hiseq X-ten system ...
-
bioRxiv - Molecular Biology 2024Quote: ... On-column DNase I digestion was performed followed by mRNA isolation with the NEBNext Poly (A) mRNA Magnetic Isolation Kit (NEB). Library preparation was done with the Ultra II Directional RNA Library Prep for Illumina following the manufacturer’s manual ...
-
bioRxiv - Biochemistry 2024Quote: ... Site-directed mutagenesis was performed using standard PCR-based mutagenic procedures with the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... with C-terminal TEV, GFP, 7xHis, and StrepII (Thawani et al., 2020) using the Q5 Site Directed Mutagenesis Kit (NEB). All three constructs were expressed using the Bac-to-Bac Sf9 cell expression system (Invitrogen) ...
-
bioRxiv - Biochemistry 2024Quote: ... a codon-optimized synthetic gene product was synthesized in two overlapping halves by Twist Biosciences and integrated into a vector using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB). pNH-TrxT was a gift from Opher Gileadi (Addgene plasmid # 26106 ...