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Citations for New England Biolabs :
651 - 700 of 10000+ citations for Mouse Tyrosinase Related Protein 1 TYRP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... and α-N-acetyl-galactosaminidase (New England Biolabs, 10 U/μg protein). Removal of sialic acids was performed using the α2-3,6,8 neuraminidase (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... Each protein was expressed in T7 Express cells (New England BioLabs, MA) grown in Terrific Broth at 37°C following induction with 0.5mM IPTG ...
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bioRxiv - Biochemistry 2022Quote: ... LEGDF-SNAPf protein was labelled with SNAP-Surface649 (Surf649, New England Biolabs). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... coli for protein purification were derivatives of pMal-c5X (New England Biolabs), which carries an AmpR marker and uses an IPTG-inducible tac promoter to expresses recombinant proteins with a factor Xa cleavable maltose-binding protein (MBP ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were expressed in Escherichia coli T7 Express (New England Biolabs) and grown in Luria-Bertani medium ...
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bioRxiv - Microbiology 2020Quote: ... protein extracts were treated with PNGase F according to manufactures instructions (NEB).
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bioRxiv - Immunology 2020Quote: The fusion protein was treated with glycerol free PNGase F (NEB, P0705S) in non-denaturing reaction conditions ...
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bioRxiv - Bioengineering 2021Quote: ... the eluted MBP-RFP-sspB proteins were cut with TEV protease (NEB) using a digestion site between the MBP and the RPF sections ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein digestion was achieved by sequential addition of endopeptidase Lys-C (BioLabs) and trypsin (Pierce™ ...
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bioRxiv - Developmental Biology 2022Quote: ... The bait protein vector was digested with AscI/BamHI (New England Biolabs) to remove the tag encoding region and then ligated to an eGFP insert generated by PCR using primers with AscI and BamHI tails (see Table S5 for primer sequences) ...
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bioRxiv - Microbiology 2019Quote: In vitro protein expression was carried out following manufacturer’s instructions (NEB, E6800s). Briefly ...
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bioRxiv - Immunology 2020Quote: ... 1x NEB buffer for Protein Metallophosphatase and 1mM MnCl2 (New England Biolabs) for 30 min at 30°C ...
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bioRxiv - Cell Biology 2021Quote: ... samples were diluted with 3x Blue Protein Loading Dye (New England Biolabs) and boiled for 5 minutes ...
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bioRxiv - Physiology 2021Quote: ... Proteins were then deglycosylated with PNGaseF (New England Biolabs, Ipswich, MA, USA) and digested with Lys-C (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was batch bound to 10 mL of chitin resin (NEB) for 1 hour at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GFP-fusion proteins were purified from BL21(DE3) cells (NEB, # C2527H) using an N-terminal H6-tag ...
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bioRxiv - Biochemistry 2020Quote: Proteins have been expressed in Escherichia coli strain BL21 (DE3) (NEB # C2527). In brief ...
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bioRxiv - Immunology 2021Quote: ... The lysate and the precipitates were treated with Lambda Protein Phosphatase (NEB) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... 1200 μg protein was mixed with 15 μL Glycoprotein Denaturing Buffer (NEB) in 150 μL total volume and incubated at room temperature for 10 min ...
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bioRxiv - Developmental Biology 2022Quote: ... while the other was treated overnight with Protein Deglycosylation Mix II (NEB) using non-denaturing reaction conditions according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... the MBP-fusion proteins were purified using amylose resin (New England Biolabs) and GST-fusions by glutathione-resin (GE Healthcare) ...
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bioRxiv - Plant Biology 2022Quote: ... incubated with λ protein phosphatase (40 units/μL) (P0753S, New England Biolabs) or recombinant TOPP4-GST/HIS for 1 h at 30□ ...
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bioRxiv - Plant Biology 2022Quote: ... MBP-fusion proteins were purified using Amylose Resin (New England Biolabs, E8021), following the manufacturer’s instruction with minor modification ...
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bioRxiv - Microbiology 2023Quote: ... and then associated with EnGen Spy Cas9 NLS protein (New England BioLabs) using the 4D-Nucleofector system (Lonza) ...
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bioRxiv - Cell Biology 2023Quote: ... 25 µl protein A magnetic beads S1425S (New England Biolabs; Ipswich, MA) were washed 3 times in PBS and incubated overnight with 2-3 µg of antibody directed against CISD34 at 4°C with gentle mix ...
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bioRxiv - Cell Biology 2023Quote: ... and MBP-tagged proteins were purified on Amylose resin (New England Biolabs).
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins were purified on glutathione-Sepharose beads (New England Biolabs), and MBP-tagged proteins were purified on Amylose resin (New England Biolabs).
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bioRxiv - Developmental Biology 2023Quote: ... sgRNA was incubated at room temperature with Cas9 protein (NEB, Cat# M0646T). AB* embryos were injected at the one-cell stage with the sgRNA and Cas9 cocktail in a 1nL volume at 25pg sgRNA/nL ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were lysed and subsequently incubated with λ-protein phosphatase (NEB) for 1 h at 30°C according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... The proteins were purified by NEBExpress Ni Spin Columns (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of 2X protein synthesis buffer (New England Biolabs, Beverly, MA), 0.25 μL of RNaseOUT RNAse inhibitor (Thermo-Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... This complex was co-injected with Cas9 protein (M0386M, NEB, Ipswich USA) into the yolk of 1-cell stage WT zebrafish embryos ...
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Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2023Quote: ... The diluted sample was treated with 2 µl Lambda protein phosphatase (NEB) and incubated in water bath at 30 °C for 4 hrs ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins with MBP-tag were loaded onto amylose resin (New England Biolabs) after elution from Ni-NTA ...
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bioRxiv - Biochemistry 2023Quote: ... 2.5 μL 10 mM MnCl2 and 2 μL Lambda Protein Phosphatase (NEB). The dephosphorylation reaction was allowed to occur at 30 °C overnight ...
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bioRxiv - Microbiology 2024Quote: ... The molecular weight standards used were unstained broad range protein standards (NEB) and then the gel was run at 100 volts until the dye front was 1 cm from the bottom of the gel and then stained one hour in Coomassie blue stain (50% methanol ...
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bioRxiv - Molecular Biology 2024Quote: ... including a Blue Pre-stained Broad Range Protein Standard (New England Biolabs). Gels were either stained (with 0.5% (w/v ...
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bioRxiv - Genetics 2021Quote: ... using the LTP library preparation kit and employing NEBNext multiplex oligos for Illumina index primer pairs set 1 (New England Biolabs, Ipswich, Massachusetts). Sequencing was performed using a NextSeq 500/550 mid-output v2.5 300 cycle cartridge (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA synthesis was performed from 1 µg total RNA using the ProtoScript® First Strand cDNA Synthesis Kit (New England Biolabs, NEB) with the provided random primer mix according to the suppliers’ instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was generated from 1 μg of total RNA using ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, #E6560).
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bioRxiv - Biophysics 2019Quote: ... HIV-1 MAG2A-ΔHBR-EGFP was generated from HIV-1 MAG2A-EGFP by deleting amino acids 18-32 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA). pEGFP-N1 and pEYFP-N1 were obtained from Clonetech (Mountainview ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Library preparations were performed using the NEBNext Ultra II DNA Library Prep Kit for Illumina with the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (NEB, Ipswich, Massachusetts). Library preparation followed the protocol outlined in Saeidi et al ...
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bioRxiv - Plant Biology 2021Quote: ... #ltp1.4ltp1.8-1 and #ltp1.4ltp1.8-2) were chosen for constructing next generation sequencing libraries following the manufacture’s protocol (NEB Next Ultra II DNA kit). Sequencing was carried out using 2 × 150 paired-end NextSeq500 (1-2 Mio reads for all samples together ...
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bioRxiv - Cancer Biology 2020Quote: ... Genes of interest were cloned into the pLenti CMVTRE3G eGFP Neo (w821-1) plasmid using a Gibson Assembly Master Mix kit (Cat# E2611S, New England Biolabs, Ipswich, MA). pLenti CMV rtTA3 Blast (w756-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA-seq libraries were prepared using 1 µg of RNA and the NEBNext Ultra™ II RNA Library Prep Kit (NEB, E7775) with rRNA depletion following the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... the truncated versions of cwr-1 and the histidine mutant plasmids were prepared using the Q5 Site-Directed Mutagenesis Kit (E0552S NEB, Ipswich, MA). Gibson assembly (E2611S NEB ...
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bioRxiv - Immunology 2021Quote: ... The linearized vector and the synthesized fragment in a 1:2 molar ratio were assembled with the NEBuilder HiFi DNA Assembly Kit (NEB, Ipswich, USA) at 50°C for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... the 181-bp and 376-bp amplicon products (Figure 1-figure supplement 1A) were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA) and sent for Sanger DNA sequencing at the OHSU Vollum Sequence Core (Portland ...
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bioRxiv - Biophysics 2023Quote: ... 1 µg of the duplex was in vitro transcribed using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB # E2050S) following the standard IVT protocol and purified using Monarch® RNA Cleanup columns (NEB # T2030).
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bioRxiv - Evolutionary Biology 2023Quote: ... we purified the products of the ace-1 PCR (see above) using the BS664-250 Preps EZ-10 Spin Column PCR Purification kit (New England BioLabs, Evry France). The purified PCR products were then cloned (TOPO TA Cloning Kit pCR 2.1-TOPO Vector and TOP10F’ invitrogen bacteria) ...