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Citations for New England Biolabs :
651 - 700 of 2969 citations for Fumarase from porcine heart since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Plasmid DNA was purified from liquid cultures using DNA miniprep kit (NEB #T1010).
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bioRxiv - Bioengineering 2022Quote: ... coli SHuffle T7 Express lysY and the restriction enzymes were purchased from NEB Japan ...
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bioRxiv - Cancer Biology 2022Quote: ... and cell pellets from our patient-derived cell lines was extracted (NEB, T3010S). Genomic libraries were prepared (Illumina ...
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bioRxiv - Biochemistry 2022Quote: Cell lines and molecular biology materials were purchased from New England Biolabs (NEB) unless otherwise indicated ...
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bioRxiv - Synthetic Biology 2022Quote: ... Restriction enzymes used for vector digests were purchased from New England Biolabs (NEB). Sanger sequencing was performed by Quintara Biosciences (Cambridge ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from single-colony cultures (Monarch Genomic DNA Purification Kit, NEB). Short read libraries were prepared based on the NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads (NEB ...
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bioRxiv - Immunology 2022Quote: ... TCRs were then amplified by PCR (20 cycles with the Phusion from NEB) with a single primer pair binding to the constant region and the adapter linked to the TRAV/TRBV primers added during the reverse transcription ...
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bioRxiv - Molecular Biology 2022Quote: ... The LacO array was excised from the pLAU43_LacO_plus vector42 with XhoI (NEB, R0146L) and ligated into the linearized targeting vector (cut with XhoI ...
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bioRxiv - Microbiology 2022Quote: ... All DNA polymerases and restriction enzymes were purchased from New England Biolabs (NEB) unless stated otherwise ...
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bioRxiv - Bioengineering 2022Quote: ... cas12a (1 μM) and ATP are purchased from NEB (New England Biolabs Inc). SYBR Green II dye ...
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bioRxiv - Cell Biology 2022Quote: ... The standard procedure and reagents from the Q5 Site-Directed Mutagenesis Kit (NEB) were used to insert mutations ...
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bioRxiv - Plant Biology 2021Quote: ... yielding almost 1.6 M mapping-quality variants (homozygous in NEB, absent from WUS). ddRADseq libraries were de-multiplexed and barcodes removed and variants called using a GATK 4.0 best practices pipeline29 ...
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bioRxiv - Developmental Biology 2021Quote: ... Grb10a was amplified from cDNA by high specificity PCR (NEB Q5 Hot Start) with primers flanking open reading frame (Table 1) ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA from each sample was treated on-column with DNase I (NEB) for 15 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Ribonucleotides (ATP, CTP, GTP and UTP) were purchased from New England Biolabs (NEB). [α–32P] ATP (3000 Ci/mmol ...
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bioRxiv - Bioengineering 2021Quote: ... (4) lacI and tac promoter from pMAL-c5X (New England Biolabs, Ipswich, MA). The modified replacement plasmid for pMut2 ...
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bioRxiv - Systems Biology 2022Quote: ... with the exception of using the commercial φ29 reaction buffer from NEB (B0269L). Amplification products were cleaned and concentrated using the E.Z.N.A.® Cycle-Pure Kit from Omega BioTek (D6492-02 ...
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bioRxiv - Cell Biology 2022Quote: ... pGEM-N-SNAPf was first constructed by removing SNAPf from pSNAPf (NEB, N9183S) with AgeI-XhoI and inserting it into the AgeI-XhoI site of pGEM-HE (51) ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from cells using Monarch total RNA Miniprep kit (NEB). cDNA synthesis was performed using LunascriptTM RT Supermix Kit (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... The pMal-c5X vector was purchased from New England Bioscience (NEB, Ipswich, MA). Tenofovir-DP ...
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bioRxiv - Genomics 2020Quote: Sequencing libraries were prepared using DNA derived from Enterobacteria phage lambda (NEB N3011S), fragmented using a g-TUBE™(Covaris® 520079 ...
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bioRxiv - Biochemistry 2020Quote: ... Restriction enzymes were purchased in their HF format from New England Biolabs (NEB); PCR stages used the KOD HotStart Polymerase Kit (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: PCR was performed using Phusion HF polymerase purchased from New England Biolabs (NEB). T4 polynucleotide kinase (T4 PNK ...
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bioRxiv - Synthetic Biology 2020Quote: ... SNAP reagents (BG-649, BG-fluorescein and BG-biotin) were obtained from NEB. Electrocompetent cells were used in all transformations.
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bioRxiv - Synthetic Biology 2020Quote: ... Enzymes and DNA/RNA ladders were purchased from New England BioLabs® (NEB), and used as per the manufacturer’s instructions unless otherwise indicated ...
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bioRxiv - Immunology 2020Quote: ... RNA was isolated from the cell culture supernatants and converted to cDNA (NEB). The E gene of stock virus ...
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bioRxiv - Synthetic Biology 2021Quote: All restriction enzymes used for molecular biology were from New England Biolabs (NEB). Synthetic oligonucleotides for cloning and sequencing were purchased from Eurofins Genomics or GENEWIZ ...
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bioRxiv - Genomics 2020Quote: ... M.HhaI (a kind gift from Dr. R. J. Roberts, New England Biolabs, USA) and M.SPRI (a generous gift from Prof ...
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bioRxiv - Microbiology 2020Quote: ... otitidiscaviarum NEB252 DNA samples were obtained from New England Biolabs (NEB, Ipswich, MA). Those for C ...
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bioRxiv - Biochemistry 2020Quote: ... restriction enzymes and T4 DNA ligase were obtained from New England Biolabs (NEB). Sanger sequencing was performed by Genewiz Incorporated.
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bioRxiv - Genomics 2021Quote: ... Libraries were prepared from HMW DNA using NEBNext Ultra II (NEB, Beverly, MA) and sequenced on the Illumina NovaSeq ...
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bioRxiv - Cell Biology 2021Quote: His-SpCas9-GFP was expressed in and purified from BL21 (DE3, NEB, C2527H) bacteria as previously described 47 ...
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bioRxiv - Cancer Biology 2020Quote: ... Q5-high Fidelity 2X master mix was used as polymerase from NEB (# M0429L). PCR condition for the first PCR was ...
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bioRxiv - Genetics 2021Quote: ... and all molecular cloning reagents were purchased from New England Biolabs (NEB, USA). Afatinib (S1011) ...
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bioRxiv - Molecular Biology 2020Quote: ... matching cuts from the restriction enzymes KpnI and SacI (New England Biolabs, NEB). pGL3-Basic was cut with these two enzymes ...
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bioRxiv - Molecular Biology 2020Quote: ... matching cuts from the restriction enzymes KpnI and SacI (New England Biolabs, NEB). pGL3-Basic was cut with these two enzymes ...
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bioRxiv - Synthetic Biology 2023Quote: ... Phusion DNA polymerase and T5 exonuclease were purchased from New England BioLabs (NEB). Sybr Safe DNA gel stain was purchased from Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... derived from the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs). RNA was then capped with 3′-Desthiobiotin-GTP and polyA-tailed using vaccinia capping enzyme and E ...
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bioRxiv - Biophysics 2023Quote: From the H6-RapA expression plasmid and pSNAP-tag(T7) plasmid (NEB #101137), we constructed a plasmid pKI1 (Addgene #199118 ...
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bioRxiv - Molecular Biology 2023Quote: ... Nucleosomes were then generated from H2A/H2B dimers and H3.1/H4 tetramers (NEB) by incubating the DNA sequences and the histones at an octamer/DNA molar ratio of 1:1.2 (in a solution containing 10 mM dithiothreitol [DTT] and 1.8 M NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... Fragments and backbone were ligated using T4 DNA ligase (Cat# M0202) from NEB. E.coli strain BL21(DE3 ...
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bioRxiv - Cancer Biology 2023Quote: ... A second round of PCR cycle (25 cycles with the Phusion from NEB) was performed to add the Illumina adapters containing the different indexes ...
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bioRxiv - Molecular Biology 2023Quote: ... and end labeling was performed using T4 PNK from NEB (cat number: M0201S) and biotin-14-dATP from Invitrogen (cat number ...
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bioRxiv - Plant Biology 2023Quote: All genes were amplified from gDNA with a Phusion high fidelity polymerase (NEB) or a platinum SuperFi II polymerase (ThermoFisher ...
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bioRxiv - Developmental Biology 2023Quote: Regulatory elements were amplified from mouse genomic DNA with Q5 polymerase (NEB, M0491) using primers listed in Table S8 and cloned into pGL4.24[luc2P/minP] (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... the lacZ cassette was amplified from pUC19 (New England Biolabs, cat. no. N3041S) using the primers oNA311 (ttgaagacaaAATGtgagaccgcagctggcacgacaggtttc ...
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bioRxiv - Molecular Biology 2024Quote: PCR amplification of the insert was done with Q5 DNA polymerase from NEB. The standard Q5 reaction contained 5 μl of 5x Q5 reaction buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... Phusion DNA polymerase and T5 exonuclease were purchased from New England BioLabs (NEB). Sybr Safe DNA gel stain was purchased from Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Linear HDR templates were amplified from this vector using Q5 DNA polymerase (NEB) and M13Forward and M13Reverse primers and purified using Zymo Research Clean and Concentrator-25 columns ...
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bioRxiv - Synthetic Biology 2024Quote: The M13mp18 single-stranded DNA scaffold was purchased from New England Biolabs (NEB) at an initial concentration of 250 µg/mL ...