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Citations for New England Biolabs :
651 - 700 of 2074 citations for 6 fluoro 5 nitroquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... and 6 cDNA libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) with 9 amplification cycles ...
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bioRxiv - Biophysics 2021Quote: Polymerised gels were removed from the coverslip chamber and placed into 6-well plates to undergo digestion in 0.2 mg/ml proteinase K (New England Biolabs) dissolved in digestion buffer (50 mM Tris pH 8.0 (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... the supernatant was passed twice at a flow rate of 0.5 mL/min through a column with 6 mL chitin beads (NEB) equilibrated with lysis buffer ...
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bioRxiv - Biophysics 2020Quote: ... The point mutants were subsequently cloned in the pNZopuAHis plasmid (C-terminal 6-HIS-tag) using EcoRV-AlnwnI (NEB) for the substrate-binding domain mutants and βamHI-AlwnI (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... 6 μM kifunensine was added to the ExpiCHO culture media at the time of transfection and Endo Hf (NEB) treatment of LC-kappa purified protein (∼10,000 U/mg protein at 4°C for 3 hours ...
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bioRxiv - Evolutionary Biology 2021Quote: ... see Supplementary Data 6) and cloned into pDHFR vector provided in the PURExpress In Vitro Protein Synthesis Kit (NEB). The translation into liposomes was done as described previously95 and the output was analyzed by Blue Native PAGE using 2% digitonin and NativePAGE Novex 4-16% Bis-Tris Protein Gel (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2020Quote: ... A 6 µL mixture containing 2 µL of 40 µM Spy Cas9 NLS protein (New England Biolabs, MA, USA), 200 ng each of five sgRNAs (in 2 µL ...
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bioRxiv - Biochemistry 2021Quote: To generate circular DNA with a single nick phagemid DNA (~2,6 μg) with or without non-denaturing bisulfite treatment was treated with 20 Units of Nt.BstNBI (New England Biolabs) for 1 hour at 37°C in 50 μl of 1X NEBuffer 3.1 followed by deactivation at 70°C for twenty minutes.
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bioRxiv - Microbiology 2021Quote: ... 4°C and the supernatant applied on a 6 ml gravity flow column with Chitin resin (New England Biolabs), equilibrated with 20 mM HEPES ...
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bioRxiv - Synthetic Biology 2022Quote: ... 8 μL of solution A and 6 μL of solution B were mixed with 16 U RNase Inhibitor (NEB), gBlock templates (2 nM each ...
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bioRxiv - Cell Biology 2022Quote: ... Cx36-SNAP transfectants were treated with 6 µM SNAP-Cell Oregon Green (New England Biolabs Inc., Boston, MA, USA) in 10% FBS DMEM for 30 min at 37°C to quench all Cx36-SNAP molecules ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was amplified in a 25 µl PCR reactions using 6 µl cDNA and 2X Master mix (NEB). The PCR cycling program was 98 °C for 30 seconds ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted from 4.3×10^6 cells using the Monarch HMW DNA Extraction Kit for Cells and Blood (New England Biolabs) with 1400 rpm lysis speed ...
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bioRxiv - Microbiology 2023Quote: ... We incubated TXTL reactions at 29 °C for 6 h and digested at 37 °C with PacI (NEB R0547S) as instructed by the provider and added water instead of PacI for the undigested control ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 µL 100 µM dNTPs, 0.3 µL 50 mg/mL BSA, 6 µL blunting enzyme mix from quick blunting kit, NEB). Prior to ligation ...
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bioRxiv - Biochemistry 2023Quote: 3 μL of the transcription and co-transcriptional reactions were added to a 6 μL mixture containing 1x DNase I reaction buffer and 0.5 U/μL DNase I (New England Biolabs). After incubation at 37°C for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... protein aliquots were subjected to PNGase F or a broad range mannosidase (α1,2/3/6) (New England Biolabs, France) digestion according to the manufacturer’s specifications ...
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bioRxiv - Genetics 2024Quote: ... in reactions containing 1X ThermoPol buffer (12.5mM tricine, 42.5mM KCl, 1.5mM MgCl2, 6% dextran; New England BioLabs, Ipswich, MA), 0.32 mM dNTP mix ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5× Q5 buffer and pUC19 plasmid as template (New England Biolabs) in 50 μl ...
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bioRxiv - Cell Biology 2020Quote: ... and further digested overnight with 5 U DpnII (New England Biolabs) at 37°C in a humidified atmosphere ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by phosphorylation of the 5’ end with T4 kinase (NEB). Phosphorylated RNA was then purified by the RNeasy kit (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... after which 5’end was repaired with T4 polynucleotide kinase (NEB). Nascent RNAs containing the adaptors were converted to cDNA ...
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bioRxiv - Genomics 2020Quote: ... with 2 μl (5 U/μl) of Klenow fragment exo- (NEB) in a final volume of 55 μl by incubation at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... The labeled oligonucleotide (∼5 pmol) was treated with uracil glycosylase (NEB) in 1x UDG buffer (20 mM Tris-HCl ...
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Direct analysis of ribosome targeting illuminates thousand-fold regulation of translation initiationbioRxiv - Molecular Biology 2020Quote: ... RNAs were 5′ capped using the Vaccinia Capping System (NEB M2080S) and 3′ biotinylated using the Pierce RNA 3′end biotinylation kit (Thermo Scientific 20160).
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bioRxiv - Molecular Biology 2020Quote: ... 5’-triphosporylated RNA was capped with 3’-desthiobiotin-TEG-GTP (NEB)) using the Vaccinia virus Capping enzyme (NEB ...
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bioRxiv - Molecular Biology 2021Quote: The size selected nascent RNA was treated with 5’ Pyrophosphohydrolase (NEB) in ThermoPol® reaction buffer following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’ ends were phosphorylated by 20 U polynucleotide kinase (PNK; NEB) by adding 1 mM ATP (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 U Taq DNA polymerase (New England Biolabs, NEB, Inc, USA) and 13 μM of each of the primers ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 U Taq DNA polymerase (New England Biolabs, NEB, Inc, USA) and 13 μM of each of the primers ...
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bioRxiv - Molecular Biology 2021Quote: ... All guides were 5′-phosphorylated using T4 PNK (New England Biolabs) except experiments with 5′-OH guides ...
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bioRxiv - Molecular Biology 2021Quote: ... 40 μl reaction mix containing 5 μl Isothermal amplification buffer (NEB), 3 μl 100 mM MgSO4 ...
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bioRxiv - Molecular Biology 2020Quote: ... then free 3 adaptors were degraded using 5 deadenylase (NEB, M0331) and RecJf (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... Escherichia coli competent cells NEB 5-alpha (C2987, New England Biolabs), NEB Stable (C3040 ...
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bioRxiv - Genomics 2020Quote: ... 5 μL of 10 U/μL T4 PNK (New England Biolabs), 4 μL of 3 U/μL NEB T4 DNA Polymerase (New England Biolabs) ...
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bioRxiv - Genetics 2020Quote: ... 5 ng RPE1 DNA was digested with HpaII (NEB, Ipswich, MA), suspended in ddPCR mixture [ddPCR Supermix (No dUTP ...
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bioRxiv - Biochemistry 2019Quote: ... and then passed over an 5-8 mL amylose column (NEB). The bound protein was washed with ten CV of Amylose Wash Buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 µM Rad51 and 10 µM (nucleotide) φX174 circular ssDNA (NEB) were mixed in reaction buffer C (30 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Genomics 2019Quote: ... the RNA products were treated with RNA 5’ Pyrophosphohydrolase (RppH, NEB) at 37°C for 30 min followed by one wash of High ...
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bioRxiv - Molecular Biology 2020Quote: ... All constructs were 5’ capped using vaccinia capping enzyme (NEB-M2080S) and polyadenylated using E ...
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bioRxiv - Genetics 2021Quote: ... 5′ Modifications of RNA were removed with RppH treatment (NEB Thermopol): 40 ul of Nuclease-free water ...
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bioRxiv - Immunology 2021Quote: ... and 375 U/mL of Klenow Fragment (3’-5’ exo-) (NEB). After cDNA synthesis and subsequent purification by AMPure XP (Beckman Coulter) ...
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bioRxiv - Genomics 2021Quote: ... 5 μL of 400 U/μL T4 DNA Ligase (NEB M0202), and incubating at room temperature for ≥4 hours with rotation.
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bioRxiv - Immunology 2021Quote: ... TAP reaction was performed using 5 μL of Q5 polymerase (NEB), 5 μL of GC Enhancer (NEB) ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was removed by adding 5 units of RNase H (NEB) and incubating for 20 min at 37 °C ...
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bioRxiv - Plant Biology 2020Quote: ... and pENTR-NPH3 5’ were digested with BtsI (New England BioLabs) and the resulting fragments were ligated using a DNA Ligation kit (Takara) ...
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bioRxiv - Evolutionary Biology 2021Quote: Replicon constructs (5 μg) were linearised with AscI (New England Biolabs) for 1 h at 37 °C and purified using the E.Z.N.A ...
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bioRxiv - Microbiology 2021Quote: ... and was then installed with 5’cap (Vaccinia Capping System, NEB, USA ...
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bioRxiv - Molecular Biology 2020Quote: ... we used 5 units of Escherichia coli RNase HII (NEB, M0288L) in place of the C ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was treated with 5 units of RNaseH (New England Biolabs) for 20 min at 37°C followed by purification with the QIAquick PCR purification kit (Qiagen) ...