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Citations for New England Biolabs :
651 - 700 of 1404 citations for 3 Chloro phenyl 9H fluoren 9 ylmethoxycarbonylamino acetic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... the serine residues S240 and S250 in Dsn1 were mutated to aspartic acid using the Q5 site-directed mutagenesis kit (New England Biolabs) as described previously 19,20 ...
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bioRxiv - Microbiology 2022Quote: ... and a subpart of it encoding specifically the PAS domain (amino acids 1-138) the corresponding coding sequence was amplified by PCR using the Phusion DNA polymerase (New England Biolabs) and cloned between the NdeI and BamHI sites ...
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bioRxiv - Molecular Biology 2021Quote: ... 8 μg of digested nucleic acids were treated or not with 10 μl of RNase H (New England BioLabs, M029L) overnight at 37 °C in 1x RNAse H buffer and 1/10 of the samples were used as input ...
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bioRxiv - Molecular Biology 2022Quote: ... Amino acid substitutions and domain deletions were made using either Gibson assembly or the Q5 Site-Directed Mutagenesis Kit (NEB). Isogenic single and double mutant strains were generated via haploid mating ...
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bioRxiv - Genomics 2021Quote: ... Site-directed mutagenesis for amino acid substitution was performed using the Q5® Site-directed mutagenesis kit (New England Biolabs) according to the manufacture’s instruction ...
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bioRxiv - Cell Biology 2021Quote: ... plasmid was made by inserting the TauRD sequence (Tau amino acids 244-371) in pHUE82 by Gibson assembly using the Gibson Assembly Master Mix (New England Biolabs). Plasmid pHUE-TauRD (C291A/P301L/C322A/V337M ...
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bioRxiv - Cell Biology 2020Quote: ... A fragment of human Plexin-B1 cDNA (encoding amino acids 1-535) was cloned into the pcDNA5 vector using HindIII (R0104S, NEB) and XhoI (R0146S ...
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bioRxiv - Cell Biology 2021Quote: ... Single or multiple amino acid mutants of TULP3 and ARL13B were generated by Q5 site directed mutagenesis (New England Biolabs). For biotinylation experiments ...
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bioRxiv - Microbiology 2024Quote: Amino acids substitutions in ompT were generated using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA). Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... containing 0.06 % pluronic acid (F-127, final concentration) and 1 µL of each disulfide bond enhancer 1 and 2 (NEB #E6820). We used droplet oil consisting of 3M™ Novec™ HFE7500 Engineered Fluid (3M ...
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bioRxiv - Microbiology 2023Quote: ... A stop codon was inserted after amino acid residue 370 by site directed mutagenesis using Q5 Site-Directed Mutagenesis Kit (NEB), in order to express the truncated N1-370 construct ...
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bioRxiv - Biochemistry 2023Quote: ... serine or aspartic acid were generated by engineering the corresponding constructs using Q5 site-directed mutagenesis (New England Biolabs, E0554). For this purpose ...
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bioRxiv - Biophysics 2023Quote: ... and 575-585 for ΔX-Y contact) were replaced with a 12 amino acid GSSG linker using the KLD enzyme mix (NEB). Expression and purification were carried out the same as for the wildtype protein ...
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bioRxiv - Biophysics 2024Quote: ... Plasmid region corresponds to the 71-1184 amino acids of BimC and the modified vector was amplified and assembled with KLD Enzyme Mix Reaction (NEB) to generate the recombinant BimC(Δ1-70)-GFP construct ...
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bioRxiv - Cell Biology 2024Quote: ... The concentration of RNA was assessed by comparing the known nucleic acid concentrations of the DNA ladder bands (NEB, N3232L). Microinjection protocol was followed as mentioned previously 19 using Nanoject II injector (Drummond Scientific Company ...
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bioRxiv - Cell Biology 2024Quote: ... was inserted into murine Shh between amino acids 92N and 93T (corresponding to N91 and T92 in human Shh) by using Gibson assembly (HiFi Assembly Kit, NEB). Where indicated ...
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bioRxiv - Cell Biology 2024Quote: ... the DNA sequence coding for amino acids 384 to 667 of Fbxo42 was cloned into pETM-30 at XhoI/NcoI (NEB) restriction sites ...
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bioRxiv - Cell Biology 2024Quote: ... a PCR product containing the sequence encoding amino acids 104-330 of MPS1 from Drosophila melanogaster was inserted into the pMal-c2 vector (New England Biolabs) by FastCloning (Li et al. ...
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bioRxiv - Plant Biology 2024Quote: ... Aspartic acid 1209 of MBD9 was mutated to Alanine through GAT→GCT DNA sequence change using the New England Biolabs (NEB) Site Directed Mutagenesis Kit and D1209A Mutagenic primers listed in Supplemental Table 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... The signal peptide was removed (amino acids 1-35) by mutagenesis using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs).
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bioRxiv - Biochemistry 2024Quote: ... A truncated version of this wild-type VgrS lacking the first 81 amino acids was created by Q5-mutagenesis (NewEngland Biolabs, NEB) to generate VgrS (residues 81-729 ...
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bioRxiv - Developmental Biology 2021Quote: ... with subsequent end repair/dA-tailing reaction using Klenow Fragment (3’-5’ exo-) (NEB M0212S) and ligation with Illumina sequencing adapters using T4 DNA ligase (NEB) ...
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bioRxiv - Developmental Biology 2021Quote: ... Top and bottom guide RNAs were annealed with T4 Polynucleotide Kinase (3’ phosphatase minus, NEB) and T4 DNA Ligase Buffer (NEB ...
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bioRxiv - Genomics 2022Quote: ... Genomic DNA was dephosphorylated by adding 3 μl of Quick CIP enzyme (NEB, Cat #M0508) to 5 μg of input DNA (in total volume of 24 μl at >210 ng/μl ...
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bioRxiv - Genomics 2020Quote: ... add 2 μl 10 mM dATP and 5 μl Klenow Fragment (3’ −> 5’ exo-) (NEB) to digest the unligated DNA fragments at 37°C for 40 min ...
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bioRxiv - Genomics 2021Quote: ... rRNA was removed from 3 replicates with NEBNext Bacteria rRNA Depletion Kit (New England Biolabs), while the other 3 were treated with NEBNext Depletion Core Reagent Set using custom probes targeted to Haloferax volcanii rRNA (Martinez-Pastor and Sakrikar ...
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bioRxiv - Genomics 2021Quote: ... The ligation mix (5 μL of LNB, 3 μL of Quick ligase (New England Biolabs), 1.2 μL of MQ ...
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bioRxiv - Pathology 2021Quote: ... The second strand was synthesized with Klenow Fragments (3′-5′ exo-; New England Biolabs Inc.) and complement chains of NSR primers ...
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bioRxiv - Neuroscience 2020Quote: ... These 3 DNA fragments were assembled into a vector backbone (NEB pMiniT vector, EcoRI linearized) with the NEBuilder HiFi DNA Assembly Cloning Kit (NEB Cat# E5520) ...
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bioRxiv - Biochemistry 2020Quote: ... was replaced with sequence for a 3×FLAG tag by Gibson Assembly (New England Biolabs) to create payload plasmid pSAB41 ...
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bioRxiv - Microbiology 2021Quote: ... coli MG1655 genomic DNA into pKK223-3 by NEBuilder HiFi DNA Assembly Cloning Kit (NEB) and the first 300 bp portion of leuA was translationally fused with the nano-Luc (nLuc ...
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bioRxiv - Genomics 2022Quote: ... dA was added to the 3’-end of DNA using Klenow fragment exo- (NEB: M0212S) and then the adapter was ligated to the end of DNA ...
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bioRxiv - Cancer Biology 2022Quote: ... 3’ ligation (on-bead) with a barcoded RNA adapter followed using T4 RNA Ligase (NEB). Samples were again stringently washed ...
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bioRxiv - Developmental Biology 2022Quote: ... and the 3’ homology arm were produced by PCR (Phusion DNA polymerase, New England Biolabs). The 5’ and 3’ homology arm DNA fragments were amplified from genomic DNA prepared from vasa-Cas9 flies ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 0.1 mM dATP and 15 units of Klenow Fragment (3’→5’ exo-) (NEB, M0212M) and incubated at 37°C for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... and 3 µg/ml leupeptin were incubate with 17.5 U alkaline phosphatase (New England Biolabs) at 37°C for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... CrPV 5’UTR-1A-GFP-3’ UTR was generated using Gibson assembly (NEB Gibson assembly). The respective mutants were generated using Site directed mutagenesis ...
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bioRxiv - Molecular Biology 2020Quote: ... 240 μM TruSeq Universal Adapter (5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’) and 0.025 U Taq DNA Polymerase (NEB) in 1X Standard Taq Reaction Buffer (NEB) ...
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bioRxiv - Genomics 2021Quote: ... 1 μl of RNAse inhibitor and 3 μl of Antarctic phosphatase (New England BioLabs Inc.). We then performed a kinase treatment adding 4 μl of T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Genomics 2021Quote: ... to 3’-P presenting in the RNA that was dephosphorylated using T4 PNK (NEB, #M0201S). Linker and RNA ligation was performed by ligating pre-adenylated linker to the 3’-OH of RNA using RNA ligase2 ...
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bioRxiv - Genomics 2021Quote: ... The 3′ adapter (sequence: AGATCG-GAAGAGCACACGTCTGAACTC) was ligated using T4 RNA Ligase 1 (NEB, M0204L) and purified nascent RNA using streptavidin beads (NEB ...
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bioRxiv - Molecular Biology 2020Quote: 3’ linker ligation (1x PNK buffer, 800 u T4 RNA ligase 2 truncated KQ (NEB), 80 u RNaseOUT ...
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bioRxiv - Molecular Biology 2020Quote: ... and 2.0 units of Klenow Fragment (3’-5’ exo−)(New England Biolabs, Ipswich, Massachusetts, USA), total 10 μl of the mixture was incubate at 37°C for 90 min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and second strand cDNA synthesis (using Klenow fragment 3’-5’ exo- [New England BioLabs, USA]) of chicken fecal samples and dust samples was performed as previously described 55 and following manufacturer’ instructions.
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bioRxiv - Cancer Biology 2022Quote: ... 3 μg purified DARPP-32 isoforms as well as 5 μl ATP (New England Biolabs) in kinase dilution buffer III (SignalChem ...
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bioRxiv - Cell Biology 2022Quote: ... A18-Y260A-SDM-Rev (5’-CGTTGTGTAACCAGGAGAATG-3’) and Q5 site directed mutagenesis kit (NEB E0554). This LT3N1-GPIR-Arhgef18 vectors were further modified to substitute EGFP with 3XHA-FLAG tag ...
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bioRxiv - Cell Biology 2022Quote: ... miRE-Rv (5’-TCTCGAATTCTAGCCCCTTGAAGTCCGAGGCAGTAGGC-3’) and Q5 Hot Start High-Fidelity DNA polymerase (NEB M049S). The final amplicon was then digested and cloned into LT3GEPIR in-between XhoI and EcoRI restriction sites as previously described2 ...
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bioRxiv - Microbiology 2024Quote: ... the pUC19-PRha-YFP-3×FLAG plasmid was inverse amplified by PCR (Q5 polymerase, NEB), omitting the yfp gene ...
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bioRxiv - Microbiology 2023Quote: ... The 3 fragments were ligated using the Gibson Assembly® Cloning Kit (New England Biolabs). The created pEXG2::ΔfahA and pEXG2::ΔpanC were transformed into E ...
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bioRxiv - Cancer Biology 2023Quote: ... 1pmol ssDNA substrate 5’ DY782 ATTATTATTATTATTATTATTTCATTTATTTATTTATTTA-3’ (Eurofins, UK) and 0.75U uracil-DNA glycosylase (NEB) were added to 10µg protein lysate at 37°C for 1 hour ...