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Citations for New England Biolabs :
6851 - 6900 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) and a CFX96 Real-Time System ...
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bioRxiv - Microbiology 2021Quote: ... The RNA-Seq libraries were prepared from total RNA using the NEBNext Ultra II RNA Sample preparation kit (NEB, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Transcription of pgRNA constructs were carried out using a Hiscribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs), after linearization of the DNA plasmid using HindIII ...
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bioRxiv - Microbiology 2021Quote: ... The Y-adaptor was ligated to the ends of the fragments using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR products were gel purified and transcribed in vitro using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). The transcribed sgRNAs were purified using the RNeasyTM mini kit (Qiagen).
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bioRxiv - Biochemistry 2021Quote: ... 1 μg of total RNA was depleted of rRNA using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) prior to cDNA synthesis primed with random hexanucleotide oligonucleotides (Random Primer 6, NEB). Sequencing library construction was carried out using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Cell Biology 2020Quote: InsertTAG-pcDNA FRT/TO was created by mutagenesis of YFP-pcDNA5 FRT/TO39 to replace YFP with NheI and XhoI restriction sites using the primers ccctcgagGATATCACAAGTTTGTACAAAAAAGC (forward) and tggctagcAAACGCTAGAGTCCGGAG (reverse) and the Q5 Site-Directed Mutagenesis Kit (E0554S, New England Biolabs). BioID2-pcDNA5 FRT/TO was created by restriction digestion of BioID2 from myc-BioID2-MCS (74223 ...
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bioRxiv - Molecular Biology 2020Quote: ... Region B or Region C were used as templates for in vitro transcription by HiScribe T7 quick high yield RNA synthesis kit (NEB). The obtained RNA products were converted back to DNA oligo probes via reverse transcription (RT ...
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bioRxiv - Neuroscience 2020Quote: ... Intron-containing dCas9-VPR was constructed by the insertion of a gBlock containing the SV40 intron into the original dCas9-VPR plasmid via Gibson assembly (Gibson Assembly kit, New England BioLabs). The SVI-FLEX construct was built via Gibson assembly of the original dCas9-VPR backbone and two gBlocks encod-ing the SV40 intron sequence ...
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bioRxiv - Molecular Biology 2021Quote: Double-stranded cDNA was prepared for Illumina sequencing with the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs) and iTru primers ...
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bioRxiv - Genomics 2021Quote: ... The library was prepared using the ONT 1D kit (SQK-LSK108) and the NEBNext End repair / dA-tailing Module NEB Blunt/TA Ligase Master Mix (New England Biolabs) according to the kit’s manual ...
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bioRxiv - Plant Biology 2020Quote: ... Sequencing libraries were prepared from 300 ng of ribo-depleted RNA (NEBNext Ultra II Directional RNA Library preparation kit) and indexed with NEB Next Illumina indexing primers (NEB). An Agilent Bioanalyzer with high sensitivity DNA chip was used to confirm the quality of the libraries before they were sent for sequencing ...
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bioRxiv - Cancer Biology 2020Quote: ... Purified fragmented mRNA was then used to generate cDNA libraries for sequencing using NEBNext Ultra Directional RNA library Kit (E7420S, NEB) following the protocol provided by the manufacturer with these modifications ...
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bioRxiv - Immunology 2020Quote: ... In vitro transcription of gRNAs from assembled oligonucleotides was conducted using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB). Injected clutches of embryos were validated to contain CRISPR mediated mutagenesis by a T7 endonuclease assay ...
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bioRxiv - Biochemistry 2020Quote: ... at 95°C for 5 min and incubated with 80000 units of O-glycosidase and 100 units of neuraminidases for 4 hours at 37°C following the NEB kit protocol (E0540S; NEB). Samples were resolved on 15% Tris-tricine SDS-PAGE gel and blotted using anti-Cterm OCN goat antibody.
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bioRxiv - Molecular Biology 2020Quote: ... Guide RNAs were cloned into a T7 promoter vector followed by in vitro transcription (HiScribe T7 High Yield RNA Synthesis Kit, New England BioLabs) and spin column purification (RNeasy ...
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bioRxiv - Genomics 2021Quote: The 5’- and 3’-adapter sequences are based on the adapters from the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (New England Biolabs) with the addition of 5 random nucleotides (5N) ...
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bioRxiv - Genomics 2021Quote: ... All fragments used to generate the backbone vector and subsequent backsplicing cassettes were cloned using the NEB Hifi assembly kit (NEB) following the manufacturers protocols.
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bioRxiv - Genomics 2021Quote: ... and NEBNext Multiplex reagents and sequencing adapters were used to prepare sequencing libraries following the Illumina NEBNext DNA library prep kit instructions (New England Biolabs). Two rounds of capture using a pool of biotinylated oligos (IDT ...
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bioRxiv - Genomics 2021Quote: ... Site-directed mutagenesis for amino acid substitution was performed using the Q5® Site-directed mutagenesis kit (New England Biolabs) according to the manufacture’s instruction ...
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bioRxiv - Genomics 2021Quote: ... or by ribodepletion with NEBNext Human/Mouse/Rat rRNA Depletion kit followed by library construction using the NEBNext Ultra II Directional RNA-Seq protocol (New England BioLabs). Illumina 2×151 paired end reads were generated either on the HiSeq 4000 or NovaSeq 6000 sequencing platforms (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... 500ng input RNA was treated using the NEBNext Human/Mouse/Rat rRNA Depletion kit and libraries were prepared following the NEBNext Ultra II Directional RNA-Seq protocol (New England BioLabs). Paired end 2×151 bp reads were produced using the HiSeq 4000 platform (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted from whole bodies with a SDS protocol and libraries were constructed using NEBNext DNA Library Prep Kits (New England Biolabs). Whole-genome sequencing was performed on Illumina Novaseq 6000 (paired-end mode ...
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bioRxiv - Genetics 2021Quote: ... and purchased from IDT (Integrated DNA Technologies) to be used in the EnGen sgRNA Synthesis Kit (New England Biolabs, E3322S). Lyophilized oligos were resuspended in molecular biology grade water to a concentration of 100 uM ...
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bioRxiv - Genetics 2021Quote: Plasmid pNR127.9 was constructed by changing the first rfk-1 stop codon in pNR9.1 from TAG to TGG with primer set 1727/1728 and the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Similarly ...
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bioRxiv - Genetics 2020Quote: ... The 5’ and 3’ sequences were amplified from WT adult gDNA extracted using the NucleoSpin Tissue Kit (Machery-Nagel) by PCR using Phusion High Fidelity PCR Master Mix (NEB) and primers 5’-ttttgcggccgcTCTGTTTGAATATGTTTCCGAGAA −3’ and 5’-ttttctcgagTTTCCGCGACAAAAACACAGA-3’ which add restriction enzyme recognition sites NotI and XhoI ...
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bioRxiv - Evolutionary Biology 2021Quote: Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA; Fig. 2). Size selection was not employed for samples with highly degraded DNA ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 μg of DNase treated RNA was then taken for cDNA synthesis using the Protoscript I first strand cDNA synthesis kit (New England Biolabs). Selected genes were amplified by quantitative real time PCR (RT-qPCR ...
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bioRxiv - Cell Biology 2021Quote: Plate-based scRNA-seq was performed with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (catalog no. E6420L; New England Biolabs). Total cell fractions from dissociated gut sections of donors BRC2033-2034 ...
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bioRxiv - Cell Biology 2021Quote: RNA used for polyA selected library generation for mRNA sequencing was isolated with the Monarch Total RNA isolation miniprep kit (New England Biolabs). Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508 ...
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bioRxiv - Genomics 2021Quote: ... and the PCR products were inserted into the SmaI site of the pPK6 vector (78) using a Gibson assembly cloning kit (New England Biolabs). This allows the expression of cloned genes under the control of the GAL1 promoter ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were inserted into the XbaI site of the pBP7 vector (78) using a Gibson assembly cloning kit (New England Biolabs). The cloned genes are placed downstream of the GAL1 promoter in the resulting plasmid constructs ...
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bioRxiv - Plant Biology 2021Quote: ... and paired-end sequencing libraries were constructed by the NEBNext Ultra DNA Library Prep Kit II for Illumina (E7370S, NEB). DNA libraries were validated again on the Agilent TapeStation 4150 ...
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bioRxiv - Immunology 2021Quote: For RNA-seq library preparation 2ug of total RNA was used to isolate mRNA using magnetic beads with mRNA isolation kit (PolyA mRNA isolation module, NEB). Later mRNA library preparation kit ...
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bioRxiv - Physiology 2021Quote: ... RNA (0.5-1 μg) was reverse-trancribed to cDNA using LunaScript RT SuperMix Kit (New England Biolabs, Ipswich, MA, USA). TaqMan gene expression assays with FAM-labeled probes were purchased from ThermoFisher Scientific (Carlsbad ...
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bioRxiv - Biochemistry 2021Quote: ... Input and RPF RNA samples were used to prepare libraries for sequencing using the NEBNext Small RNA Library Prep Kit (NEB) per manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... RAD fragments were enriched with a streptavidin bead pull-down and prepared as a sequencing library using a NEBNext Ultra DNA Library Prep Kit (New England Biolabs). Final libraries were sequenced using paired-end 150 bp sequencing on an Illumina HiSeq 4000 at Duke University's Center for Genomic sand Computational Biology sequencing facility.
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bioRxiv - Cell Biology 2021Quote: Genotyping PCR was performed on genomic DNA (isolated using a PureLink™ Genomic DNA Mini Kit according to manufacturer’s protocol) with Phusion (NEB) or taq (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA libraries were generated by the Skoltech Genomics Core Facility using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instructions and sequenced on Miseq (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... The Y-adaptor was ligated to the ends of the fragments using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The pbp2x 5’-end and 3’-end amplicons were spliced with the linearized pBBL740 amplicon using NEBuilder HiFi kit (New England Biolabs). The resultant spliced plasmid was transformed into parental strain MGAS27213-L601P and single cross-over transformants were selected by plating on THY agar with chloramphenicol 10ug/ml ...
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bioRxiv - Physiology 2021Quote: ... ChIP-seq libraries were prepared from 1 to 5 ng eluted DNA using NEBNext Ultra II DNA library Prep Kit (New England BioLabs), with 12 cycles of library amplification ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1B) driving intestinal Pun-PGP-9 expression were assembled using the NEB HIFI DNA Assembly Kit (New England Biolabs Inc.) according to the manufacturer’s instructions into the supplied pUC19 vector linearized by SmaI (ThermoFisher ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... The LPHN-1 and -3 genes were amplified by PCR and digested by T7 endonuclease using the EnGen Mutation Detection Kit (New England Biolabs) according to directions in combination with the custom primers that flank the appropriate CRISPR-targeting regions (Fig ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) and a CFX96 Real-Time System ...
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bioRxiv - Biochemistry 2021Quote: ... pET-28a(+)::aprt2-Ntag and pET-28a(+)::aprt2-Ctag were linearized by PCR (S2 Appendix) and cloned into the pPICZ vectors (pPICZ-Ntag and pPICZ-Ctag) using a HiFi DNA assembly kit (NEB). Assembled products were subsequently transformed into NEB 5-alpha competent Escherichia coli cells using heat-shock and incubated overnight at 37°C on LB agar plates containing kanamycin 50 μg mL-1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Synthesis of cDNA from the RNA subsamples was carried out with First Strand cDNA Synthesis Kit (ProtoScript® II; NEB) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... and used here as a template to obtain mutants C204S and W195D using the Q5 mutagenesis kit (New England Biolabs) and to subclone construct Arpin_CA (residues 194-226) ...
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bioRxiv - Biochemistry 2021Quote: The Saccharomyces cerevisiae Mtr4ΔF was constructed by removing residues 667-813 and inserting a three-glycine linker using the Q5 Site-Directed Mutagenesis Kit (NEB). All Mtr4 proteins were recombinantly expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL (Stratagene ...
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bioRxiv - Biochemistry 2021Quote: Site directed mutagenesis for Ser247Thr mutation on hMAT2A plasmid and Thr227Ser mutation on eMAT plasmid was carried out using Q5 Site-Directed Mutagenesis Kit (NEB) by following kit protocol and expressed ...