Labshake search
Citations for New England Biolabs :
6601 - 6650 of 9366 citations for Rat Alanine Glyoxylate Aminotransferase AGXT ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: A construct expressing SURF4 and the Katushka2S fluorescent marker (PGK-SURF4-p2A-Katushka2S) was assembled with the NEBuilder HiFi DNA assembly cloning kit (NEB) using vector sequence derived from LV1-5 (Addgene #68411 ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... was used as an input for library preparation and libraries were prepared using NEB ultra II DNA library prep kit (NEB). Sequencing reads (76 bp PE ...
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bioRxiv - Evolutionary Biology 2021Quote: ... mRNA libraries were constructed using the kits NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina (New England BioLabs) following the manufacturer protocol ...
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bioRxiv - Systems Biology 2021Quote: ... Fragment end repair and adaptor ligation was performed using an NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 16 μl of SSS reaction products were combined with 24 μl HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) reagent mix containing 4 μl T7 Buffer ...
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bioRxiv - Genomics 2021Quote: Poly(A)+ sequencing libraries were generated from total RNA using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer’s instruction ...
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bioRxiv - Biophysics 2020Quote: ... A Pol I construct containing an additional L361A mutation was generated from the WT construct by site-directed mutagenesis using a Q5 site-directed mutagenesis kit (NEB) according to manufacturer’s protocol ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... V796A mutations were inserted into the GFP-α-catenin plasmid using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs).
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bioRxiv - Pathology 2021Quote: Reverse-transcription polymerase chain reaction (RT-PCR) was performed using the Luna Universal Probe One-Step RT-qPCR kit (NEB). Two gene targets were used for SARS-CoV-2 RNA detection ...
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bioRxiv - Neuroscience 2022Quote: ... Sheared DNA fragments was Subjected to Illumina paired-end DNA library using NEBNext ultra II DNA Library Prep Kit (E7645L, NEB). Preparation and PCR-amplified for three cycles and libraries size were selected with 0.55-1X magnetic beads (Ampure XP ...
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bioRxiv - Biophysics 2021Quote: ... In vitro transcription on the DNA substrates was performed by using HiScribe T7 High Yield RNA synthesis kit (E2040S, New England BioLabs) for 120 min at 37 °C in the presence of 1 mM GTP ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA synthesis was carried out using AMV First Strand cDNA Synthesis Kit following manufacturer’s instructions (New England BioLabs. Ipswich, MA). PCR amplification was performed using Expand High Fidelity PCR System (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... and microRNAs were isolated by size selection on 6% TBE PAGE gels 1mM (Novex) with clean up performed using Monarch PCR and DNA clean up kit (NEB), according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: Detection of 5mC content at particular CCGG sites was performed using the Epimark 5hmC and 5mC analysis kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: Mutagenesis of the binding sites for the predicted Caudal and DEAF-1 transcription factors was performed sequentially with the Q5 Site-Directed Mutagenesis kit (New England Biolabs) taking as a template the pGreenRabbit vector containing the FBti0019386 sequence following manufacturer’s instructions (25) ...
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bioRxiv - Biophysics 2020Quote: ... The construct encoding for the EC2 domain of CD9 (CD9EC2, residues K114 – N191) was generated with a PCR reaction using the Q5-PCR kit (NEB). The CD9EC2 construct was cloned in a pUPE expression vector with a N-terminal cystatin signal peptide and a C-terminal 6x-His tag ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA template was in vitro transcribed using the HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs). The sgRNA was subsequently isolated using the phenol-chloroform extraction/NH4OAc precipitation method (Nakayama et al. ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed and total RNA was isolated with the Monarch RNA isolation kit (New England Biolabs, Ipswich, MA, USA), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... A Ty tag DNA sequence was inserted at the 3’-end of the gat1 coding sequence using a Q5 site directed mutagenesis kit (NEB) and Fn and Rn primers ...
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bioRxiv - Genetics 2021Quote: ... yoaALexAp1 5’-GCGCCCTCAT CCTGACATAA TGTCCCTTCA AATCAAGGGA CGGTAGTGTG ACGGAC-3’ and yoaALexAp2 5’-GTCCGTCACA CTACCGTCCC TTGATTTGAA GGGACATTAT GTCAGGATGA GGGCGC-3’ and amplification with the Phusion High-Fidelity DNA Polymerase PCR kit (New England BioLabs). Constructs were sequence verified.
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bioRxiv - Microbiology 2021Quote: ... The DNA fragments were repaired and ligated to adapters with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). Following restriction of the Tn with BamHI (New England Biolabs) ...
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bioRxiv - Genomics 2021Quote: ... DNA targets were ordered from Integrated DNA Technologies and in vitro transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs). Transcriptions were performed according to the manufacturer’s recommendations with a reaction volume of 20 μL that was incubated overnight at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... cDNA libraries with unique barcodes were generated from 100 ng enriched mRNA using NEB Next UltraII Directional RNA Library Prep kit for Illumina (New England Biolabs), then assessed for quality and quantity by Qubit ...
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bioRxiv - Genomics 2021Quote: PCR amplification of the samples was done following the suggestions from NEBNext Ultra II library prep kit for Illumina (ER7645, New England Biolabs), using the NEBNext® Multiplex Oligos for Illumina®(E7337A ...
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bioRxiv - Microbiology 2020Quote: ... An aliquot of each PCR reactions was checked by gel electrophoresis for single amplicons before purifying the remaining reaction using Monarch DNA Gel Extraction Kit (NEB), per manufacturer instructions ...
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bioRxiv - Genomics 2020Quote: ... 25 uL of LNB buffer from LSK-109 kit (Oxford Nanopore Technologies) and 10 uL of ligase (New England Biolabs) and and 5 uL of adapter from LSK-109 kit was then added the reaction mixture and mixed by gentle tapping ...
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bioRxiv - Cancer Biology 2020Quote: ... 30 ng total RNA (RQI≥8) was used for library preparation following the NEBNext Ultra RNA Library Prep Kit for Illumina protocol (New England BioLabs) with the Poly(A ...
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bioRxiv - Genomics 2020Quote: ... Directional RNA-seq libraries were prepared from 1000 ng total RNA per sample using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs), with initial polyA+ isolation ...
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bioRxiv - Genomics 2019Quote: Sequencing libraries were prepared as previously described (Belsky et al. 2015) with the following modifications: NEBNext multiplex oligos for Illumina kit (New England Biolabs) were used in adapter ligation and PCR steps ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we used it for metagenomic library preparation using NEBNext Ultra II DNA Library Prep Kit for Illumina (BioLabs, New England), with the target insert length of 350 bp ...
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bioRxiv - Genomics 2019Quote: ... The library was prepared using the NEBNext Ultra II kit and indexes from the Dual Index Primers Set 2 (all New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: RNA was extracted from 0.25×106 cells at day 12 of cell culture using the Monarch total RNA miniprep kit (New England Biolabs, T2010S) and quantified using the Qubit RNA HS Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The remaining steps of library preparation were done using the NEBNExt Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Sequencing was performed on an Illumina HiSeq 4000 with 100 bp paired-end reads.
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bioRxiv - Immunology 2020Quote: ... Resulting mRNA was prepared for RNA-seq using a NEB Ultra II RNA Sequencing Library Prep Kit (New England Biolabs) as per the manufacturer’s guidelines.
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bioRxiv - Microbiology 2021Quote: ... reverse: 5’-GATGGCGTGGAACCATGTC-3’) were obtained from the wild type plasmids pCMV-hnCoV-S via Q5 SiteDirected Mutagenesis Kit (NEB). pCMV-hnCoV-S-H501Y-Δ69/70 was obtained from pCMV-7.1-hnCoV-S-H501Y (forward ...
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bioRxiv - Molecular Biology 2021Quote: ... a 4946 bp fragment generated by PCR with primers Upf2-425-fwd and Upf2-425-rev containing a the entire UPF2 gene was inserted in to pRS425 cut with NotI and SalI using the NEBuilder High-Fidelity DNA Assembly Cloning Kit (New England Biolabs).
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bioRxiv - Microbiology 2020Quote: ... electrophoresed and the 200-bp bands were excised from agarose gel slice using Monarch DNA Gel extraction Kit (NEB, #T1020S). Then ...
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bioRxiv - Microbiology 2020Quote: ... 1-2 μg of total RNA from each sample (or complete sample if the yield was lower) was used for mRNA isolation (Magnetic mRNA Isolation Kit, NEB). First strand cDNA synthesis was performed using the SuperScript III First-Strand Synthesis System and a 1:1 mix of Oligo(dT ...
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bioRxiv - Microbiology 2020Quote: ... separate reactions were performed for the quantification of SARS-CoV-2 N and E gene transcripts as well as cellular RNaseP for normalization using the Luna Universal Probe One-Step RT-qPCR Kit (NEB) on a Bio-Rad CFX384 Touch system ...
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bioRxiv - Microbiology 2020Quote: BsaI digested fragment were gel purified by using Gel Extraction Kit (OMEGA) and ligated with T4 ligase (New England Biolabs) at room temperature for 1h ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were assembled from fragments (linearized vector, PCR products, and/or synthetic DNA) using the NEBuilder Hifi DNA assembly kit (New England Biolabs (NEB) E2621) ...
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bioRxiv - Microbiology 2020Quote: ... fragments were amplified by PCR with primers oJMP197 and oJMP198 from a 78 nt oligonucleotide followed by digestion with BsaI-HF-v2 and purification with the Monarch DNA purification kit (NEB) following the manufacturer’s oligonucleotide purification protocol ...
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bioRxiv - Microbiology 2020Quote: ... were created by replacing the entire env-coding sequence of F-MLV WT with amphotropic env-coding sequence from p-SV-A MLV env using NEBuilder HiFi DNA Assembly Kit (New England Biolabs). The primers used for swapping the env-coding regions were ...
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bioRxiv - Microbiology 2020Quote: ... subtilis 168 CA with the pJM14 integrative vector carrying the yxiD-yxxD flanking sites inserted using Gibson assembly kit from NEB. For inter-species competition L ...
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bioRxiv - Microbiology 2020Quote: ... Both mCherry and mEGFP were cloned with no linker after the Matrix domain in Gag via Gibson assembly with the HiFi DNA Assembly cloning kit (New England Biolabs). Similar constructs have been previously published ...
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bioRxiv - Plant Biology 2021Quote: ... The miR823-resistant CMT3 construct (rCMT3) was generated by PCR site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs) using the cCMT3 construct as a template to introduce six silent mutations as shown in Fig ...
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bioRxiv - Microbiology 2019Quote: ... cassette between SalI and BglII in the plasmid pGDB34 was isolated by digestion and gel purification and inserted between the same restriction sites in the plasmid pyPM2GT-EXP2-mNeonGreen14 with a T4 Quick Ligation kit (NEB), replacing the yDHODH cassette ...
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bioRxiv - Immunology 2021Quote: ... In vitro transcribed RNA was generated using the HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, Cat E2060S) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Illumina-compatible libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (catalog no. E7645S; New England BioLabs) and sequenced using an Illumina NovaSeq 6000 system in a paired-end 2x50- base pair (bp ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The extracted RNA was prepared for short-read sequencing library construction using the NEB NextUltra RNA-seq kit (New England Biolabs). The constructed libraries were sequenced with 150bp paired-end reads on an Illumina HiSeq 2500 and 6000 sequencing platforms ...