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Citations for New England Biolabs :
601 - 650 of 10000+ citations for Rat C Reactive Protein ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... and α1-3,4 fucosidase (New England Biolabs, 4 U/μg protein). All enzymatic reactions were performed as a 1-step reaction with 1x Glycobuffer 2 (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... Molecular weights were determined using protein standards (New England Biolabs P7719).
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bioRxiv - Molecular Biology 2021Quote: ... The protein was then digested with LysC endoproteinase (0.25 µg; NEB) for 4 hours at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... MBP fusion proteins were purified on amylose beads (New England Biolabs) in column buffer (20mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was first bound to amylose resin (New England Biolabs). Afterwards ...
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bioRxiv - Cancer Biology 2019Quote: ... After cooling 5 µl Protein Deglycosylation Mix II (New England Biolabs) was mixed in gently ...
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bioRxiv - Pathology 2021Quote: ... Soluble protein extracts were purified using amylose resin (New England Biolabs) and/or Ni-NTA agarose beads (Life Technologies).
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bioRxiv - Microbiology 2020Quote: ... Magnetic beads conjugated with IgG Protein A/G (New England Biolabs) were pre-blocked with 0.5 mg/mL of sonicated salmon sperm DNA (Thermo Scientific ...
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bioRxiv - Biophysics 2021Quote: ... 100μg/ml single stranded DNA binding protein (gp32) from T4 (NEB), 277μg/ml T4 DNA clamp ...
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bioRxiv - Biochemistry 2021Quote: ... the purified proteins were incubated with excess streptavidin (New England Biolabs) for 10 minutes before analysis via gel-shift using SDS-PAGE ...
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bioRxiv - Developmental Biology 2021Quote: ... Cas9 protein (2.12 ng) (New England BioLabs Inc. Ipswich, MA, USA,) and gRNA (133 pg ...
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bioRxiv - Developmental Biology 2021Quote: ... Purified sgRNA and Cas9-NLS protein (New England Biolabs® Inc.) were diluted to 300ng/ul.
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bioRxiv - Molecular Biology 2021Quote: ... Samples were then added to protein A magnetic beads (NEB, S1425S) and rotated at 4°C for 4 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were purified using amylose resin column (New England Biolabs). Fractions were eluted and checked by silver staining ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were induced in SHuffle Express strains (New England Biolabs) upon addition of 1mM IPTG in cultures containing kanamycin 50 μg/mL and incubated with agitation at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: Protein extract samples (5µl) were subjected to EndoHf (New England Biolabs) treatment according to manufactures instructions ...
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bioRxiv - Genomics 2022Quote: ... EnGen Spy Cas9 NLS protein (20 μM) was purchased from NEB. The injection mixture consisted of 600 ng/μl Cas9 protein ...
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bioRxiv - Genetics 2022Quote: ... 0.8pg of 3xNLS-SpCas9 protein and 1mU of I-SceI (NEB) was injected per embryo at early 1-cell stage ...
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bioRxiv - Plant Biology 2022Quote: ... and MBP-HIS-GFP proteins were purified with amylose resin (NEB) and SUMO-HIS-BIN2 protein was purified with Ni-NTA Agarose (Qiagen).
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bioRxiv - Molecular Biology 2022Quote: ... 2) 7 µL of Protein Deglycosylation Mix II (NEB, cat. P6044S) per slide was added for deglycosylation and incubated overnight at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... for GST fusion proteins or amylose resin (New England Biolabs #E8021S) for MBP fusion proteins for 1 hour at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... then proteins were eluted in 30μL 3x Sample Loading Buffer (NEB) at room temperature for 10 minutes.
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bioRxiv - Cell Biology 2023Quote: ... A mixture containing 600 ng/uL of Cas9 protein (NEB: M0386) and sgRNA complex at a 1:1 ratio was generated with a total volume of 3 uL ...
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bioRxiv - Neuroscience 2023Quote: ... Select samples were treated on-bead with lambda protein phosphatase (NEB) in the manufacturer’s PMP buffer to remove phosphorylation ...
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bioRxiv - Microbiology 2023Quote: ... MBP-fused proteins were purified from supernatants using amylose resin (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and sizes were estimated using Blue Protein Standard (New England Biolabs). N-terminally 6×His-tagged fusions of human and Drosophila SARM1 TIR have an expected molecular weight of 19.2 kDa.
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bioRxiv - Biochemistry 2023Quote: ... MBP fusion proteins were purified on amylose beads (New England Biolabs) in column buffer (20 mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.5) and 4000 U lambda protein phosphatase (New England Biolabs) added per 1 mg digested lysate ...
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bioRxiv - Genomics 2023Quote: ... and 200 ng/µl Cas 9 protein (New England BioLabs, M0386T). Injected individuals were screened (see below genotyping procedure ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were detected using anti-MBP antibody (anti-MBP NEB e8032s) and HRP conjugated secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 μl of Lambda protein phosphatase (P0753L, New England BioLabs) were incubated with the proteins at 30°C for 30 minutes.
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bioRxiv - Molecular Biology 2023Quote: ... Protein-containing fractions were pooled and incubated with Chitin Resin (NEB) for 30 min at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... SNAP-tagged proteins were labelled with Cell-SiR647 (NEB, Frankfurt, Germany). Labeling was done at RT for 1hr ...
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bioRxiv - Neuroscience 2023Quote: ... 5 µL of Colour Prestained Protein Standard (New England Biolabs #P7719S) was included on each gel ...
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bioRxiv - Neuroscience 2024Quote: ... sgRNAs were mixed with Cas9 protein (M0646M NEB, or B25641 invitrogen) and coinjected into one-cell stage embryos using a microinjector (Eppendorf) ...
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bioRxiv - Biochemistry 2024Quote: ... Dephosphorylation reactions were performed with Lambda Protein Phosphatase (Lambda PP) (NEB). 1,000 units of Lambda PP were utilized per µmol of Ycf1 protein in a 500 µL reaction ...
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bioRxiv - Biochemistry 2024Quote: Purified MBP-fusion proteins were treated with TEV-protease (NEB, P8112S) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was expressed using the strain T7express lysY (NEB, C3010I). Expression was induced with 0.4 mM IPTG at OD600 ∼0.6 at 16°C ...
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bioRxiv - Cell Biology 2024Quote: ... were combined with protein kinase buffer (PK buffer, New England Biolabs: 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... each protein construct was transformed in either BL21 DE3 pLysS (NEB) harbouring a truncated OGT version with an intact catalytic core (OGTSW ...
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bioRxiv - Microbiology 2019Quote: PCR mixtures were digested for 1 h at 37 °C with DpnI (New England Biolabs) to remove the template plasmids ...
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bioRxiv - Neuroscience 2021Quote: ... Aβ40 fibrils were digested using the Endoproteinase Lys C enzyme (New England BioLabs, Ipswich, MA) at a 1/50 (w/w ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR product was digested with DpnI for 3h at 37°C (New England Biolabs) and then 5 µl was transformed into Stbl3 competent cells (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... the sample was ligated overnight at 16°C using T4 DNA ligase (New England BioLabs).
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bioRxiv - Microbiology 2021Quote: ... The C-tailing reaction was performed by using terminal transferase (TdT) buffer (New England Biolabs), CoCl2 ...
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bioRxiv - Bioengineering 2021Quote: ... for 16 cycles annealing at 67 °C was mixed with 1 μL of ExoI (NEB) digested mixture as the template ...
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bioRxiv - Cancer Biology 2021Quote: ... were incubated at 37 °C for 2 hours in 1x T4 DNA ligase buffer (NEB). Following this incubation 1200 units T4 DNA ligase (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... Restriction digest was performed overnight at 37°C (950 rpm) using 800 units MboI (NEB) per 5 million cells ...
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bioRxiv - Evolutionary Biology 2020Quote: ... samples were digested for 15 min at 37 °C with 5 μL of ExoI (NEB) to remove first round primers ...
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bioRxiv - Biochemistry 2021Quote: ... for 1 hr at 37 °C and Proteinase K (4 U; P8107S, NEB, Ipswich, MA) for another 2 hr at 55 °C ...