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Citations for New England Biolabs :
601 - 650 of 1846 citations for 6 Bromopyridine 2 boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 2-3 ×106 cells were treated with MNase (NEB M0247) ranging from 10-30 Kunitz U in 800 μl of custom buffer (Tris-HCl pH 7.5 10 mM ...
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bioRxiv - Genetics 2022Quote: ... and 2 μL of lambda protein phosphatase (NEB, Catalog #P0753S) were added to the washed FRQ-coupled V5 resin ...
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bioRxiv - Microbiology 2022Quote: ... (2 µg enzyme in 100 µL H2O) (New England Biolabs) at 37 °C overnight ...
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bioRxiv - Synthetic Biology 2022Quote: ... and 2 units of Phusion High-Fidelity DNA Polymerase (NEB) in HF Phusion buffer ...
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bioRxiv - Plant Biology 2022Quote: ... 1mM DTT) and treated with 2 units of CIP (NEB) at 37°C for 15mins ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.62 µL of 2 mg/mL BSA (NEB #B9001S) with the following thermal cycle condition ...
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bioRxiv - Bioengineering 2023Quote: ... Q5® High-Fidelity 2×Master Mix (New England Biolabs) was used in all the PCR reactions ...
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bioRxiv - Microbiology 2022Quote: ... 2 mM Vanadyl Ribonucleoside Complex (Cat: S1402S; New England Biolabs), and 10 U/mL SUPERase•In RNase Inhibitor (Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μL of peptide N-glycosidase F (New England Biolabs) were added ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 U/µl T4 RNA Ligase 2 truncated KQ (NEB), 2 µM HDVLig (pre-adenylated using a 5’ adenylation kit (NEB)) ...
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bioRxiv - Microbiology 2023Quote: ... and 2 μl of T4 RNA ligase (New England Biolabs) and mixed ...
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bioRxiv - Immunology 2023Quote: ... 2 mM ribonucleoside vanadyl complex (New England Biolabs, Ipswich, MA), 0.02% RNase free bovine serum albumin (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... 2 μL of Monarch RNase A (New England Biolabs (NEB)) ...
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bioRxiv - Genomics 2023Quote: ... using NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541S). The 2nd round PCR mixture was prepared and purified similarly to the 1st ...
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bioRxiv - Biochemistry 2022Quote: ... were expressed in Rosetta 2 (DE3) pLysS competent cells (NEB) grown in modified Terrific Broth at 27°C for 7 hours ...
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bioRxiv - Genomics 2023Quote: ... 2 μL of Monarch RNase A (New England Biolabs (NEB)) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 2 U/mL DNase I (New England Biolabs, M0303L). Cells were passed through a 40 µm mash and seeded at high density in BME.
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bioRxiv - Cancer Biology 2023Quote: ... and 1 μl Bst 2 WarmStart DNA polymerase (NEB M0538S) was added and sample incubated 30 min at 65°C before precipitation with 12.5 μl 10 M NH4OAc ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 U of DNase I-XT (New England Biolabs, USA) was added into 10 μL of reaction mixture and incubation was extended for 30 min at 37°C to remove the DNA templates ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... we performed 2 reactions per sample (20 µl 5× NEB Q5 buffer ...
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bioRxiv - Genomics 2023Quote: ... The APOBEC reaction mix (2 μL APOBEC reaction buffer (NEB), 0.4 μL APOBEC (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... and was loaded onto 2 mL of chitin resin (NEB) pre-equilibrated with lysis buffer ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: ... and samples were then individually diluted in NEBuffer 2 (NEB) and fragmented by incubating at 92 °C for 1.5 min ...
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bioRxiv - Genomics 2023Quote: ... The APOBEC reaction mix (2 μL APOBEC reaction buffer (NEB), 0.2 μL APOBEC (NEB) ...
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bioRxiv - Genomics 2023Quote: ... (2) +28 μl Dam at 8 U/μl (NEB M0222L); (3 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 units of Phusion High-Fidelity DNA Polymerase (NEB) in 1X Phusion HF buffer to a total volume of 100 µL ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of DNA polymerase I (10 U/μL, NEB) and 0.5 μL RNase H (2 U/μL ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 μl 10× Template Switching RT Enzyme Mix (NEB) were added to the mixture and the reaction was incubated at 42 °C for 90 min followed by 85 °C for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 mM ribonucleoside-vanadyl complex (New England Biolabs, # S1402S)) ...
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bioRxiv - Genomics 2024Quote: The reaction is supplemented with 2 μl Exonuclease V (NEB) and 5 μl of ATP 10 mM ...
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bioRxiv - Biophysics 2019Quote: ... HIV-1 MAG2A-ΔHBR-EGFP was generated from HIV-1 MAG2A-EGFP by deleting amino acids 18-32 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA). pEGFP-N1 and pEYFP-N1 were obtained from Clonetech (Mountainview ...
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bioRxiv - Microbiology 2021Quote: ... nucleic acid (5 μL) was used as template for reverse transcription using LunaScript® RT SuperMix Kit (New England Biolabs, Hitchin, UK) in 20 μL reaction volume ...
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bioRxiv - Microbiology 2021Quote: ... The first EcoRI restriction site at 829 bp within the AgeI and KasI restriction sites in RGD4C-AAVP-TNF was deleted in two steps to mutate a thymidine to cytosine nucleotide at position 833 without altering the translated amino acid using the Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich, MA) by following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: The viral nucleic acids detected by PCR of the LTR-gag region were digested with the restriction endonuclease ScaI (New England BioLabs, Beverly, MA), which cuts within the amplicon of HIV-2287 but not SIVmne ...
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bioRxiv - Biochemistry 2023Quote: Amino acid sequencing and glycan localization were carried out by digesting the antibody samples with trypsin or chymotrypsin (New England Biolabs, Ipswich, MA) followed by LC-MS/MS analysis of proteolytic fragments with an Orbitrap Fusion (Thermo ...
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bioRxiv - Evolutionary Biology 2021Quote: 5’ phosphorylation was performed by mixing 6 uL of rRNA depleted RNA with 1 uL of 10X PNK buffer (NEB B0201S), 1 uL of PNK enzyme (NEB M0236S) ...
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bioRxiv - Systems Biology 2021Quote: ... PE2 indexing barcodes were then added by amplifying 2 microliters of the previous reaction with forward primer P1_outer and reverse primers PE2_outer_SIC69 and PE2_outer_SIC70 (Supplementary file 6) for 5 cycles at an annealing temperature of 66C followed by 5 cycles with no annealing step (NEB Q5) and then purified with the Monarch PCR kit.
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bioRxiv - Systems Biology 2021Quote: ... P1 indexing barcodes were added using forward primers P1_inner_A through P1_inner_D and reverse primer P1_inner_nested_rev (Supplementary file 6) for 5 cycles at an annealing temperature of 55C followed by 5 cycles with no annealing step (NEB Q5). PE2 indexing barcodes were then added by amplifying 2 microliters of the previous reaction with forward primer P1_outer and reverse primers PE2_outer_SIC69 and PE2_outer_SIC70 (Supplementary file 6 ...
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bioRxiv - Systems Biology 2021Quote: ... The final enrichment PCR used primers MO588 and MO589 (Supplementary file 6) for 20 cycles at an annealing temperature of 66C (NEB Phusion), followed by purification with the Monarch PCR kit ...
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bioRxiv - Cancer Biology 2020Quote: ... and gene expression was analyzed by RT-qPCR on QuantStudio 6 (Applied Biosciences) using the Luna Universal qPCR kit (New England Biolabs, M3003). Relative expression was normalized to Actb and Gapdh housekeeping genes and was determined using the ΔΔCt method ...
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bioRxiv - Genomics 2020Quote: ... and then used as the template for an additional 6 cycles of PCR amplification with NEBNext i5 and i7 primers (NEB #E7600S).
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bioRxiv - Genomics 2021Quote: NGS libraries were generated by amplifying the CUT&Tag DNA fragments with i5 and i7 barcoded HPLC-grade primers (Buenrostro et al., 2015) (Supplementary Table 6) with NEBNext® HiFi 2x PCR Master Mix (New England BioLabs) on a thermocycler with the following program ...
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bioRxiv - Immunology 2019Quote: ... activated and transduced T cells were incubated with GXR-B27 cells pulsed with the indicated peptide concentrations for 6 hours in presence of Brefeldin A (NEB; 420601). The cells were stained with anti-LNGFR-PE ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription reactions were performed with 6 μl of purified RNA and random primers using the NEB ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560S), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 510 ng (6 µl at 85 ng/µl) of DNA was digested using endonucleases EcoRI and MseI (New England BioLabs, Inc.) after which barcoded (EcoRI cut site ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were made with 6 ng of CUT&RUN DNA fragments using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S). The manufacturer’s protocol was adjusted to account for shorter DNA fragments as described previously (33) ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were made with 6 ng of CUT&RUN DNA fragments using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S). The manufacturer’s protocol was adjusted to account for shorter DNA fragments as described previously 45.
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bioRxiv - Cancer Biology 2023Quote: Total RNA was collected from parental iOvCa147 cells and DYRK1A−/−cells from 24-hour adherent or 6-hour spheroid conditions using the Monarch Total RNA Miniprep Kit (NEB #T2010S) as described above ...
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bioRxiv - Cell Biology 2023Quote: ... For second strand cDNA synthesis 6 μL of second strand reaction mix was added [1× NEBNext Second Strand Synthesis buffer (NEB #E6111S), NEBNext Second Strand Synthesis Enzyme Mix ...
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bioRxiv - Cancer Biology 2023Quote: ... sgRNA barcodes were amplified by PCR using the gDNA from at least 6 mio cells as template and Phusion (NEB M0530) as polymerase ...