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Citations for New England Biolabs :
6351 - 6400 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Experiments with PURExpress In Vitro Protein Synthesis Kit (NEB, E6800) were performed as per the manufacturer’s instructions using 10 ng/µL of DHFR-Strep reporter plasmid with the addition of 0.8 U/µL RNase Inhibitor Murine (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... total RNA was isolated using Monarch RNA prep kit (NEB). Reverse transcription was performed on 0.5µg DNase-treated total RNA using Lunascript RT (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... the NEBNext Poly(A) mRNA Magnetic Isolation Kit (NEB, E7490) was employed to capture mRNA ...
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bioRxiv - Neuroscience 2024Quote: ... with NEB Next Library Quant Kit for Illumina (NEB, E7630L) and KAPA Library Quantification Kit (Roche ...
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bioRxiv - Genetics 2024Quote: ... Libraries were prepared using Ultra II RNA library kit (NEB) and samples were sequenced on the NovaSeq 6000 platform (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... 5 and 7dpi using a total RNA extraction kit (NEB) and following the kit instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The NebNext® UltraTM II DNA library prep kit (NEB) for Illumina was used for sequencing library preparation following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared according to manufacturer’s instructions using the following kits: NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® and NEBNext® Multiplex Oligos for Illumina® (New England BioLabs) and pooled in equimolar quantities ...
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bioRxiv - Molecular Biology 2022Quote: ... Ribosomal RNA was depleted of using the NEBNext® rRNA Depletion Kit and cDNA libraries were prepared using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). RNA was barcoded using the NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... PCR protocols entailed an initial phase of 95° C for 3 min using Hot Start Taq polymerase (New England BioLabs Inc., Ipswich, MA, USA), followed by 35 cycles at 50°C and 52°C annealing temperatures for 16S and ITS amplicons respectively ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 µl of the dissolved cells were added to a 50 µl PCR reaction (Q5 DNA polymerase, NEB; see Table S2 for primer sequences) and amplified with 36 rounds of thermal cycling ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We used primers specific to the RNA oligos and performed RACE analysis by nested RT-PCR using Q5 High-Fidelity DNA polymerase (New England BioLabs Japan Inc., Tokyo, Japan). The primers specific to dsx and dsx-like were made from sequences of the relevant genomic regions and are listed in supplementary table 9 ...
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bioRxiv - Plant Biology 2021Quote: ... the PCR reaction profile and program were setup as above with the following modifications: Q5 high-fidelity DNA polymerase (NEB, Frankfurt am Main, Germany) and 5x Q5 reaction buffer were used for PCR amplification ...
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bioRxiv - Genetics 2022Quote: ... and lysates were used as gDNA templates for PCR amplification with OneTaq® Quick-Load® 2X Master Mix with Standard Buffer (all materials from New England BioLabs, Ipswich, MA). Genotyping was performed with primers listed in Supplemental Table 6 with the following thermocycling conditions ...
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bioRxiv - Developmental Biology 2020Quote: ... Aliquots from these lysates were used for a PCR (with a Taq DNA polymerase with standard Taq buffer NEB or EconoTaq DNA polymerase Lucigen) with respective gene primers and an 18mer M13F-FAM fluorescent tagged primer (5’-TGTAAAACGACGGCCAGT-3’ ...
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bioRxiv - Microbiology 2021Quote: ... The PCR amplification was carried out with Phusion High-Fidelity PCR Master Mix with HF Buffer in 50uL reactions with 20ng of genomic DNA (New England Biolabs Inc., Ipswich, MA, USA). Reactions were run in an Agilent SureCycler 8800 (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We used primers specific to the RNA oligos and performed RACE analysis by nested RT-PCR using Q5 High-Fidelity DNA polymerase (New England BioLabs Japan Inc., Tokyo, Japan). The primers specific to dsx and dsx-like were made from sequences of the relevant genomic regions and are listed in Table 6 ...
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bioRxiv - Bioengineering 2020Quote: ... 2–3 μl of genomic DNA was used as a template in a 50-μL PCR reaction with Q5® High-Fidelity 2X Master Mix (NEB, Ipswich, MA, USA). The following primers (Table S1 ...
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bioRxiv - Molecular Biology 2021Quote: ... sequence of iRFP670 amplified from piRFP670-N1 vector by polymerase chain reaction (PCR) using Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA) and primers encoding NcoI and XmaI digestion sites (Supplementary Table S1) ...
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bioRxiv - Bioengineering 2022Quote: ... 1ul of genomic DNA was used as a template in a 20ul PCR reaction using Q5 HotStart DNA polymerase (NEB, Cat. No./ID: M0493L). Each individual was genotyped for the presence of the y-chromosome (42) ...
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bioRxiv - Bioengineering 2022Quote: ... 1 µl of genomic DNA was used as a template in a 20 µl PCR reaction using Q5 HotStart DNA polymerase (NEB, Cat. No./ID: M0493L) and primers 1154A.S5 and 1154A.S7 amplifying genomic Fle sequences ...
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bioRxiv - Plant Biology 2023Quote: ... Site directed mutagenesis of MLO2CT was performed by Gibson assembly (Gibson et al. 2009) based on suitable PCR fragments generated with Phusion® high-fidelity DNA polymerase (NEB GmbH, Frankfurt, Germany). The CAM2 coding sequence was inserted as an NcoI/XhoI DNA fragment into modified pET28a vector that lacks the N-terminal His6 tag (previously designated pETλHIS ...
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bioRxiv - Bioengineering 2023Quote: ... All 5μl of the cell lysis were used for each Genomic PCR amplification using NEBNext® Ultra™ II Q5® Master Mix (NEB, M0544S). PCR program is 1 cycle ...
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bioRxiv - Synthetic Biology 2023Quote: ... All PCR steps where the product was used in a cloning reaction were performed with Q5 High-Fidelity polymerase (NEB, Ipswich, MA, Cat#M0492S). After confirming the PCR product size with gel electrophoresis the PCR product was incubated with DpnI (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative RT-PCR was conducted on a Roche Lightcycler 480 instrument using Luna Universal qPCR master mix (New England Biolabs in Ipswich, MA, USA). The geometric mean of recA and gyrB transcripts was used to normalise data ...
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bioRxiv - Plant Biology 2020Quote: ... mRNA was isolated with Oligo-dT Beads from NEB and RNAseq libraries were constructed with the Directional Kit from NEB. Sequencing was performed at IPS2 Paris ...
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bioRxiv - Synthetic Biology 2021Quote: ... The reaction was set up using a commercial kit (PURExpress NEB) according to user guidelines ...
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bioRxiv - Cancer Biology 2021Quote: ... The NEBNext ultra II RNA library prep kit (New England Biolabs) was used to generate poly(A)+ libraries ...
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bioRxiv - Microbiology 2020Quote: ... the overhanging ends were blunted using a blunting enzyme kit (NEB) and an A-tail was added with Taq DNA polymerase (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... using the NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). Allelic replacement of the hlyA open reading frame by this constructs ...
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bioRxiv - Developmental Biology 2021Quote: ... Mutagenic primers and the Q5 site-directed mutagenesis kit (NEB E0554S) were used to generate the BD-Chinmonon-stop and the CG11180/Chigno-sim* mutant ...
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bioRxiv - Cell Biology 2019Quote: ... The NEBNext Ultra II RNA Library Prep Kit (New England Biolabs) was used to generate TruSeq-barcoded RNA-Seq libraries ...
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bioRxiv - Evolutionary Biology 2019Quote: ... using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs) and the online tool NEBasechanger to design the mutagenesis primers ...
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bioRxiv - Microbiology 2020Quote: ... The ChiZ overexpression construct was engineered using the NEBuilder kit (NEB), according to the manufacturer’s instructions with primers PA311 + PA312 and LW043 + LW044 and the pBRplac-lacI derivative ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA fragment ends were repaired using Quick Blunting™ kit (NEB), purified with QIAquick column (Qiagen ...
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bioRxiv - Developmental Biology 2021Quote: ChIP-seq libraries were made following the NEBnext UltraII kit (NEB) and associated protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA libraries were prepared using the E7765L mRNA kit (NEB next). Paired-end read sequencing was performed using the HiSeq2500 instrument ...
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bioRxiv - Neuroscience 2022Quote: ... Assembly was done using NEB HiFI DNA assembly kit (NEB E5520) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Ribosomal RNA was depleted using the NEBNext rRNA depletion kit (NEB) following the product instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... MeRIP was performed using the EpiMark N6-methyladenosine Enrichment Kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and the NEBuilder HiFi DNA assembly cloning kit (New England Biolabs), respectively ...
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bioRxiv - Cell Biology 2022Quote: ... ARF1 point mutations were generated by Quick-change Mutagenesis kit (NEB) and KLD reactions ...
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bioRxiv - Cell Biology 2022Quote: ... and treated with the NEBNext rRNA depletion kit (New England Biolabs) to deplete the ribosomal RNA (rRNAs) ...
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bioRxiv - Microbiology 2020Quote: ... For 3’ adaptor ligation the NEBNext Small RNA Kit (E7560S, NEB) was used ...
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bioRxiv - Immunology 2021Quote: ... The adaptors from the NEBNext Multiplex Oligos Kits (NEB, Ipswich, MA) were ligated to the adenylated 3’ ends of the DNA fragments ...
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bioRxiv - Genomics 2020Quote: ... A quick Ligation Kit (New England Biolabs Inc., MA, CA#M200l) was used for ligating the insert and vector ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA synthesis was performed using the (AMV cDNA kit, NEB, E6550S) according to the manufacturer’s protocol using random primers and 0.1-3 µg of total RNA per sample according to the experiment ...
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bioRxiv - Microbiology 2019Quote: ... and Monarch Total RNA Miniprep Kit (New England Biolabs, Ipswich, MA) following the supplier protocols ...
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bioRxiv - Biochemistry 2020Quote: ... then RNAs were purified using a Monarch RNA Cleanup kit (NEB), or homemade equivalent buffers and regenerated columns,75 following the same rationale ...
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bioRxiv - Developmental Biology 2019Quote: ... Library preparation (New England Biolabs Nest Ultra RNA library prep Kit) and single read Next generation sequencing (Illumina HiSeq 2500 ...