Labshake search
Citations for New England Biolabs :
6201 - 6250 of 7781 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2023Quote: ... and 1 U of Taq DNA polymerase (OneTaq, New England Biolabs). The PCR conditions included an initial denaturation step at 94°C for 3 min ...
-
bioRxiv - Cell Biology 2023Quote: ... 1mM DTT) before adding 15 µl 1 mg/ml Streptavidin (NEB) before immediate wash with DLB-C-T buffer (DLB supplemented with 1 mg/ml α-casein ...
-
bioRxiv - Molecular Biology 2023Quote: ... or 8 µl RNase III (1:1000 diluted, New England Biolabs) or RNase If (1:100 diluted ...
-
bioRxiv - Cancer Biology 2024Quote: ... made up of 1 uL DNA Ladder (New England Biolabs, N3231L), 1 µL Purple Dye (New England Biolabs ...
-
bioRxiv - Microbiology 2024Quote: ... and 1 μL of terminal transferase (20 units, NEB Catalog #M0315S) was added to the reaction with incubation at 37 °C for 1 h to block any pre-existing strand-break sites ...
-
bioRxiv - Molecular Biology 2024Quote: ... followed by the addition of 1 μl M13KO7 helper phage (NEB).
-
bioRxiv - Microbiology 2024Quote: ... (b) Heat labile Shrimp Alkaline Phosphatase (rSAP) (1 µl) (NEB #M0371S) was used to remove terminal phosphates that could interfere with terminal extension by incubating in rCutSmart buffer (3.5 µl ...
-
bioRxiv - Systems Biology 2024Quote: ... IVT products were treated with 1 U of DNase I (NEB) at 37°C for 15 min and brought to a final volume of 100 µL with nuclease-free water ...
-
bioRxiv - Genomics 2024Quote: ... 16ul nuclease-free water and 1 μl T4 DNA ligase (NEB). The entire volume of the reaction was pipetted on a parafilm sheet placed on a glass plate and the coverslips were inverted over it ...
-
bioRxiv - Genomics 2024Quote: ... Unmethylated λ-bacteriophage DNA (1:200 mass ratio; New England Biolabs) was included in all samples to calculate unmethylated cytosine conversion efficiency ...
-
bioRxiv - Microbiology 2024Quote: ... 1 µl (500 Units) of PNGase F (New England BioLabs, Inc.) was used to deglycosylate 10 µg of the protein in the presence of 5 mM DTT and mild detergents by incubating at room temperature for 1 hour according to manufacturer’s recommendations.
-
bioRxiv - Synthetic Biology 2024Quote: ... 1 µl of Golden Gate Enzyme Mix (BsaI-HFv2, NEB M2616AA), and adding dH2O to reach a total volume of 20 µl ...
-
bioRxiv - Microbiology 2024Quote: ... respectively) using T4 RNA Ligase 1 (New England Biolabs, USA; #M0204) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... and 1 μL of terminal transferase (20 units, NEB Catalog # M0315S) was added to the reaction with incubation at 37 °C for 1 h to block any pre-existing strand-break sites ...
-
bioRxiv - Genetics 2024Quote: ... 1 µL (FC 1.67 µM) Cas9 protein (New England Biolabs, Cat.M0646) was freshly prepared on the morning of the injection procedure ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 1 µl T4 DNA ligase (400 units/µL; New England Biolabs), and 1.5 µl 10x T4 DNA ligase buffer ...
-
bioRxiv - Neuroscience 2024Quote: ... brains were incubated in SNAP-Surface 647 (1:1000; NEB; S9136S) or CLIP-Surface 647 (1:1000 ...
-
bioRxiv - Genetics 2024Quote: ... and 1 µL CutSmartTM r3.1 Buffer (New England BioLabs, MA, USA) was incubated for 2 hours at 37 °C ...
-
bioRxiv - Microbiology 2024Quote: ... DNAs were incubated with 1 µl EcoRI in Buffer 2.1 (NEB). Reaction mixtures were treated with Proteinase K for 30 minutes at 55°C and products were separated by electrophoresis on 1xTBE 15% polyacrylamide gels ...
-
bioRxiv - Synthetic Biology 2024Quote: ... with or without 1 µL/reaction of PNGase F (NEB, P0704S) and incubated for 1 h at 37°C ...
-
bioRxiv - Bioengineering 2024Quote: ... NEBNext Multiplex Oligos for Illumina (Index Primer Set 1) (NEB, 7335S) were used for the final PCR amplification ...
-
bioRxiv - Molecular Biology 2024Quote: ... Coverslips were then washed twice in 1× rCutSmart buffer (NEB; #B6004S) and twice in 1×T4 ligase buffer (NEB ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 1 U of α2-3,6,8,9 neuraminidase (NEB; Cat. No. P0722L), 50 mM phosphate buffer ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 mM ATP with 20 U of T4 PNK (NEB, M0201). Following an ethanol precipitation ...
-
bioRxiv - Biochemistry 2024Quote: ... and 1 µL of Thermostable RNase H (New England Biolabs, M0523S) before incubation at 55 °C for 15 minutes ...
-
bioRxiv - Genomics 2024Quote: ... a respective combination of 1 µL FPG (NEB, 8 U/µL), 1 µL ENDOIV (NEB ...
-
bioRxiv - Genomics 2024Quote: ... 1 µL T4 polynucleotide kinase (T4 PNK) (10 U/µL, NEB) was added to the gDNA in 1x NEBuffer 2 (NEB ...
-
bioRxiv - Genomics 2024Quote: ... using the 1 kb plus DNA ladder (New England Biolabs, N3200S). High molecular weight samples were retained for subsequent steps ...
-
bioRxiv - Synthetic Biology 2024Quote: ... coli NGF-1 gDNA using Q5 High-Fidelity PCR polymerase (NEB) following the manufacturer’s guidelines ...
-
bioRxiv - Genomics 2024Quote: ... DNA was mixed with 1 µL FPG (NEB, 8 U/µL) and 1 µL T4 PNK (NEB ...
-
bioRxiv - Bioengineering 2024Quote: ... 1 U/µL Murine RNAse Inhibitor (New England Biolabs, Cat #M0314L), 0.002 U/µL yeast inorganic pyrophosphatase (NEB ...
-
bioRxiv - Cell Biology 2024Quote: ... 1 unit (100nM) Casein Kinase II (CK2) (New England Biolabs, P6010SVIAL) and/or recombinant human GSK3 beta (Abcam ...
-
bioRxiv - Bioengineering 2024Quote: ... and 1 µg of gDNA per sample was DpnI-digested (NEB) to remove residual donor plasmid ...
-
bioRxiv - Cell Biology 2024Quote: ... reverse-transcribed (1 μg, LunaScript RT Supermix 5X; New England Biolabs), and analyzed by quantitative PCR (qPCR ...
-
bioRxiv - Genetics 2024Quote: ... Reactions were incubated with 1 µL Themolabile Exonuclease I (NEB, M0568L) at 10 min at 37° ...
-
bioRxiv - Genetics 2021Quote: ... 1 ul of 20 μM Cas protein (SpyCas9 and SauCas9 from NEB; Nme2Cas9 was purified as previously described (Edraki et al ...
-
bioRxiv - Genetics 2021Quote: ... and the sample was digested with DNase I (NEB [1:20 dilution]) for 30 seconds at room temperature ...
-
bioRxiv - Molecular Biology 2020Quote: ... followed by adding 1 μL of proteinase K (800 units/ml) (NEB) and incubating at room temperature for 10 minutes before being run on a 1.7% agarose gel with a 100 bp ladder for 35 minutes.
-
bioRxiv - Molecular Biology 2021Quote: ... and together with the addition of apyrase (25 mU mL−1, NEB). No CID2886111 was supplied in the purification ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 1 μl ProtoScript II RT (200 U/μl, New England Biolabs) to a total volume of 20 μl ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 U Q5 hot start high fidelity DNA polymerase (New England Biolabs), 500 nM each primer (Table 2 primers #1-4 for 1116NS19.9 or primers #5-8 for 5b1 ...
-
bioRxiv - Molecular Biology 2020Quote: ... 70 μl of end-repair mix was added (1× Ligation buffer (NEB), 357 μM dNTPs ...
-
bioRxiv - Genomics 2020Quote: ... Chromatin was digested in situ using restriction enzyme CviKI-1 (NEB, R0710L) and barcoded using RELACS custom barcodes (4bp UMI + 8bp RELACS barcode ...
-
bioRxiv - Microbiology 2020Quote: ... 0.8 mg ml-1 bovine serum albumin (BSA, New England Biolabs GmbH), 0.2 mM of each dNTP (Bioline ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and anti-Phospho-histone H3 1:100 (New England Biolabs, Ipswich, USA) and secondary antibodies AlexaFluorTM 488 goat anti-chicken and AlexaFluorTM 594 goat anti-rabbit IgG (Life Technologies ...
-
bioRxiv - Cancer Biology 2021Quote: ... The membrane was incubated with HRP-conjugated secondary antibodies (1:5000, NEB) for 45 mins at RT ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 1 μl of T4 RNA ligase I (10U final, NEB M0437M) were combined in 20 μl total volume and incubated for 16 h at 16 °C ...
-
bioRxiv - Microbiology 2021Quote: ... 1 μl of 10-mM deoxynucleoside triphosphate mix (NEB, Ipswich, Massachusetts, USA), 1 μl of each 25-μM primer ...
-
bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-SNAP (1:1000, polyclonal, New England Biolabs, Ipswich, MA, P9310S), or mouse anti-tubulin (1:40,000 ...
-
bioRxiv - Biochemistry 2021Quote: MtDNA was treated with 1 μl uracil-DNA glycosylase (UDG) (NEB, M0280), 1.5 μl Endonuclease IV (NEB ...